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University of Massachusetts Medical School

Cellular and Molecular Physiology

1992

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Poly(A) Rna Codistribution With Microfilaments: Evaluation By In Situ Hybridization And Quantitative Digital Imaging Microscopy, Krishan L. Taneja, Lawrence M. Lifshitz, Fredric S. Fay, Robert H. Singer Dec 1992

Poly(A) Rna Codistribution With Microfilaments: Evaluation By In Situ Hybridization And Quantitative Digital Imaging Microscopy, Krishan L. Taneja, Lawrence M. Lifshitz, Fredric S. Fay, Robert H. Singer

Open Access Articles

The distribution of poly(A) RNA has been visualized in single cells using high-resolution fluorescent in situ hybridization. Digital imaging microscopy was used to quantitate the signal in various cellular compartments. Most of the poly(A) signal remained associated with the cellular filament systems after solubilization of membranes with Triton, dissociation of ribosomes with puromycin, and digestion of non-poly(A) RNA with ribonuclease A and T1. The actin filaments were shown to be the predominant cellular structural elements associating with the poly(A) because low doses of cytochalasin released about two-thirds of the poly(A). An approach to assess the ...