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University of Massachusetts Medical School

Cellular and Molecular Physiology

Imbalzano Lab Publications

Articles 1 - 4 of 4

Full-Text Articles in Life Sciences

Promoter-Enhancer Looping At The Pparγ2 Locus During Adipogenic Differentiation Requires The Prmt5 Methyltransferase, Scott E. Leblanc, Qiong Wu, Pallavi Lamba, Said Sif, Anthony N. Imbalzano Jun 2016

Promoter-Enhancer Looping At The Pparγ2 Locus During Adipogenic Differentiation Requires The Prmt5 Methyltransferase, Scott E. Leblanc, Qiong Wu, Pallavi Lamba, Said Sif, Anthony N. Imbalzano

Imbalzano Lab Publications

PPARγ2 is a critical lineage-determining transcription factor that is essential for adipogenic differentiation. Here we report characterization of the three-dimensional structure of the PPARγ2 locus after the onset of adipogenic differentiation and the mechanisms by which it forms. We identified a differentiation-dependent loop between the PPARγ2 promoter and an enhancer sequence 10 kb upstream that forms at the onset of PPARγ2 expression. The arginine methyltransferase Prmt5 was required for loop formation, and overexpression of Prmt5 resulted in premature loop formation and earlier onset of PPARγ2 expression. Kinetic studies of regulatory factor interactions at the PPARγ2 promoter and enhancer revealed enhanced ...


The Ppargamma Locus Makes Long-Range Chromatin Interactions With Selected Tissue-Specific Gene Loci During Adipocyte Differentiation In A Protein Kinase A Dependent Manner, Scott E. Leblanc, Qiong Wu, A. Rasim Barutcu, Hengyi Xiao, Yasuyuki Ohkawa, Anthony N. Imbalzano Jan 2014

The Ppargamma Locus Makes Long-Range Chromatin Interactions With Selected Tissue-Specific Gene Loci During Adipocyte Differentiation In A Protein Kinase A Dependent Manner, Scott E. Leblanc, Qiong Wu, A. Rasim Barutcu, Hengyi Xiao, Yasuyuki Ohkawa, Anthony N. Imbalzano

Imbalzano Lab Publications

Differentiation signaling results in reprogramming of cellular gene expression that leads to morphological changes and functional specialization of a precursor cell. This global change in gene expression involves temporal regulation of differentiation-specific genes that are located throughout the genome, raising the idea that genome structure may also be re-organized during cell differentiation to facilitate regulated gene expression. Using in vitro adipocyte differentiation as a model, we explored whether gene organization within the nucleus is altered upon exposure of precursor cells to signaling molecules that induce adipogenesis. The peroxisome proliferator-activated receptor gamma (PPARgamma) nuclear hormone receptor is a master determinant of ...


Prmt7 Is Dispensable In Tissue Culture Models For Adipogenic Differentiation, Yu-Jie Hu, Said Sif, Anthony N. Imbalzano Dec 2013

Prmt7 Is Dispensable In Tissue Culture Models For Adipogenic Differentiation, Yu-Jie Hu, Said Sif, Anthony N. Imbalzano

Imbalzano Lab Publications

Protein arginine methylation is a common posttranslational modification that has been implicated in numerous biological processes including gene expression. The mammalian genome encodes nine protein arginine methyltransferases (Prmts) that catalyze monomethylation, asymmetric dimethylation, and symmetric dimethylation on arginine residues. Protein arginine methyltransferase 7 (Prmt7) is categorized as a type II and type III enzyme that produces symmetric dimethylated arginine and monomethylated arginine, respectively. However, the biological role of Prmt7 is not well characterized. We previously showed that Prmt5, a type II Prmt that associates with Brg1-based SWI/SNF chromatin remodeling complex, is required for adipocyte differentiation. Since Prmt7 also associates ...


Brg1, A Swi/Snf Chromatin Remodeling Enzyme Atpase, Is Required For Maintenance Of Nuclear Shape And Integrity, Anthony N. Imbalzano, Karen M. Imbalzano, Jeffrey A. Nickerson Sep 2013

Brg1, A Swi/Snf Chromatin Remodeling Enzyme Atpase, Is Required For Maintenance Of Nuclear Shape And Integrity, Anthony N. Imbalzano, Karen M. Imbalzano, Jeffrey A. Nickerson

Imbalzano Lab Publications

We recently reported that reducing the levels of BRG1, the catalytic subunit of mammalian SWI/SNF chromatin remodeling enzymes, induces alterations in nuclear shape in a breast epithelial cell line. Immunostaining the BRG1 knockdown cells with nuclear lamina antibodies revealed a significantly increased frequency of grooves, or invaginations, in the nuclei. Disruption of each of the major cytoplasmic filament systems (actin, tubulin and cytokeratins) had no impact on the BRG1-dependent changes in nuclear shape, indicating that the observed changes in nuclear morphology are unlikely to be a result of alterations in the integrity of the nuclear-cytoplamic contacts in the cell ...