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Full-Text Articles in Life Sciences

Forage News [2019-10], University Of Kentucky Department Of Plant And Soil Sciences Oct 2019

Why We Can’T Solve The Opioid Problem, Wayne F. Coombs, Ph.D. Sep 2019

Why We Can’T Solve The Opioid Problem, Wayne F. Coombs, Ph.D.

Journal of Appalachian Health

Appalachia’s opioid epidemic is a complex, systemic problem being addressed by limited intervention processes conceptualized through narrow disciplinary models that are not working. We need a new comprehensive, collaborative approach if we ever hope to find solutions to this problem.


Forage News [2019-09], University Of Kentucky Department Of Plant And Soil Sciences Sep 2019

Extracellular Vesicle-Mediated Macrophage Activation: An Insight Into The Mechanism Of Thioredoxin-Mediated Immune Activation, Chontida Yarana, Hannah Thompson, Luksana Chaiswing, D. Allan Butterfield, Heidi L. Weiss, Subbarao Bondada, Sara S. Alhakeem, Suriyan Sukati, Daret K. St. Clair Sep 2019

Extracellular Vesicle-Mediated Macrophage Activation: An Insight Into The Mechanism Of Thioredoxin-Mediated Immune Activation, Chontida Yarana, Hannah Thompson, Luksana Chaiswing, D. Allan Butterfield, Heidi L. Weiss, Subbarao Bondada, Sara S. Alhakeem, Suriyan Sukati, Daret K. St. Clair

Toxicology and Cancer Biology Faculty Publications

Extracellular vesicles (EVs) generated from redox active anticancer drugs are released into the extracellular environment. These EVs contain oxidized molecules and trigger inflammatory responses by macrophages. Using a mouse model of doxorubicin (DOX)-induced tissue injury, we previously found that the major sources of circulating EVs are from heart and liver, organs that are differentially affected by DOX. Here, we investigated the effects of EVs from cardiomyocytes and those from hepatocytes on macrophage activation. EVs from H9c2 rat cardiomyocytes (H9c2 EVs) and EVs from FL83b mouse hepatocytes (FL83 b EVs) have different levels of protein-bound 4-hydroxynonenal and thus different immunostimulatory ...


Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano Aug 2019

Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano

Plant and Soil Sciences Faculty Patents

Compositions and methods for reducing the level of nornicotine and N'-nitrosonomicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use ...


Forage News [2019-08], University Of Kentucky Department Of Plant And Soil Sciences Aug 2019

Hippodamia Convergens Overwintering Feeding, Nathan Mercer Jul 2019

Hippodamia Convergens Overwintering Feeding, Nathan Mercer

Lady Beetle Research Data

Impact of food supplements on overwintering Hippodamia convergens energy reserves, survival and spring reproduction.


Forage News [2019-07], University Of Kentucky Department Of Plant And Soil Sciences Jul 2019

Phospholipases D: Making Sense Of Redundancy And Duplication, Andrew J. Morris Jun 2019

Phospholipases D: Making Sense Of Redundancy And Duplication, Andrew J. Morris

Internal Medicine Faculty Publications

Why have two genes when one would suffice? Evolutionary pressure means that biology, unlike government, is generally intolerant of wasted effort. Therefore, when multiple genes exist presumably they are there to provide some benefit to the organism even if that benefit is not immediately obvious to us scientists. A recent report from Raghu and colleagues (Biosci. Rep. (2018) 38, pii: BSR20181690) [1] sheds some light on one possible reason for the existence of two Phospholipases D genes in chordates when only one is present in invertebrates.


Microrna Regulation Of Epigenetic Modifiers In Breast Cancer, Brock Humphries, Zhishan Wang, Chengfeng Yang Jun 2019

Microrna Regulation Of Epigenetic Modifiers In Breast Cancer, Brock Humphries, Zhishan Wang, Chengfeng Yang

Toxicology and Cancer Biology Faculty Publications

Epigenetics refers to the heritable changes in gene expression without a change in the DNA sequence itself. Two of these major changes include aberrant DNA methylation as well as changes to histone modification patterns. Alterations to the epigenome can drive expression of oncogenes and suppression of tumor suppressors, resulting in tumorigenesis and cancer progression. In addition to modifications of the epigenome, microRNA (miRNA) dysregulation is also a hallmark for cancer initiation and metastasis. Advances in our understanding of cancer biology demonstrate that alterations in the epigenome are not only a major cause of miRNA dysregulation in cancer, but that miRNAs ...


Msphere Of Influence: The View From The Microbiologists Of The Future, Aaron Mitchell, Ira Blader, Patricia Bradford, Sarah E. F. D'Orazio, W. Paul Duprex, Craig D. Ellermeier, Ana Fernandez-Sesma, Michael J. Imperiale, Katherine Mcmahon, Marcela F. Pasetti, Susannah Tringe Jun 2019

Msphere Of Influence: The View From The Microbiologists Of The Future, Aaron Mitchell, Ira Blader, Patricia Bradford, Sarah E. F. D'Orazio, W. Paul Duprex, Craig D. Ellermeier, Ana Fernandez-Sesma, Michael J. Imperiale, Katherine Mcmahon, Marcela F. Pasetti, Susannah Tringe

Microbiology, Immunology, and Molecular Genetics Faculty Publications

No abstract provided.


Development Of Cs-Tpp-Dsrna Nanoparticles To Enhance Rnai Efficiency In The Yellow Fever Mosquito, Aedes Aegypti, Ramesh Kumar Dhandapani, Dhandapani Gurusamy, Jeffrey L. Howell, Subba Reddy Palli Jun 2019

Development Of Cs-Tpp-Dsrna Nanoparticles To Enhance Rnai Efficiency In The Yellow Fever Mosquito, Aedes Aegypti, Ramesh Kumar Dhandapani, Dhandapani Gurusamy, Jeffrey L. Howell, Subba Reddy Palli

Entomology Faculty Publications

Mosquito-borne diseases are a major threat to human health and are responsible for millions of deaths globally each year. Vector control is one of the most important approaches used in reducing the incidence of these diseases. However, increasing mosquito resistance to chemical insecticides presents challenges to this approach. Therefore, new strategies are necessary to develop the next generation vector control methods. Because of the target specificity of dsRNA, RNAi-based control measures are an attractive alternative to current insecticides used to control disease vectors. In this study, Chitosan (CS) was cross-linked to sodium tripolyphosphate (TPP) to produce nano-sized polyelectrolyte complexes with ...


Genome Of The Tropical Plant Marchantia Inflexa: Implications For Sex Chromosome Evolution And Dehydration Tolerance, Rose A. Marks, Jeramiah J. Smith, Quentin Cronk, Christopher J. Grassa, D. Nicholas Mcletchie Jun 2019

Genome Of The Tropical Plant Marchantia Inflexa: Implications For Sex Chromosome Evolution And Dehydration Tolerance, Rose A. Marks, Jeramiah J. Smith, Quentin Cronk, Christopher J. Grassa, D. Nicholas Mcletchie

Biology Faculty Publications

We present a draft genome assembly for the tropical liverwort, Marchantia inflexa, which adds to a growing body of genomic resources for bryophytes and provides an important perspective on the evolution and diversification of land plants. We specifically address questions related to sex chromosome evolution, sexual dimorphisms, and the genomic underpinnings of dehydration tolerance. This assembly leveraged the recently published genome of related liverwort, M. polymorpha, to improve scaffolding and annotation, aid in the identification of sex-linked sequences, and quantify patterns of sequence differentiation within Marchantia. We find that genes on sex chromosomes are under greater diversifying selection than autosomal ...


Climate Change Impacts On Winter Wheat Yield In Northern China, Xiu Geng, Fang Wang, Wei Ren, Zhixin Hao Jun 2019

Climate Change Impacts On Winter Wheat Yield In Northern China, Xiu Geng, Fang Wang, Wei Ren, Zhixin Hao

Plant and Soil Sciences Faculty Publications

Exploring the impacts of climate change on agriculture is one of important topics with respect to climate change. We quantitatively examined the impacts of climate change on winter wheat yield in Northern China using the Cobb–Douglas production function. Utilizing time-series data of agricultural production and meteorological observations from 1981 to 2016, the impacts of climatic factors on wheat production were assessed. It was found that the contribution of climatic factors to winter wheat yield per unit area (WYPA) was 0.762–1.921% in absolute terms. Growing season average temperature (GSAT) had a negative impact on WYPA for the ...


Ubiquitin-Mediated Proteasome Degradation Regulates Optic Fissure Fusion, Warlen Pereira Piedade, Sydney Veith, Jakub Konrad Famulski Jun 2019

Ubiquitin-Mediated Proteasome Degradation Regulates Optic Fissure Fusion, Warlen Pereira Piedade, Sydney Veith, Jakub Konrad Famulski

Biology Faculty Publications

Optic fissure fusion is a critical event during retinal development. Failure of fusion leads to coloboma, a potentially blinding congenital disorder. Pax2a is an essential regulator of optic fissure fusion and the target of numerous morphogenetic pathways. In our current study, we examined the negative regulator of pax2a expression, Nz2, and the mechanism modulating Nlz2 activity during optic fissure fusion. Upregulation of Nlz2 in zebrafish embryos resulted in downregulation of pax2a expression and fissure fusion failure. Conversely, upregulation of pax2a expression also led to fissure fusion failure suggesting Pax2 levels require modulation to ensure proper fusion. Interestingly, we discovered Nlz2 ...


Xx Sex Chromosome Complement Promotes Atherosclerosis In Mice, Yasir Alsiraj, Xuqi Chen, Sean E. Thatcher, Ryan E. Temel, Lei Cai, Eric M. Blalock, Wendy Katz, Heba M. Ali, Michael C. Petriello, Pan Deng, Andrew J. Morris, Xuping Wang, Aldons J. Lusis, Arthur P. Arnold, Karen Reue, Katherine L. Thompson, Patrick Tso, Lisa A. Cassis Jun 2019

Xx Sex Chromosome Complement Promotes Atherosclerosis In Mice, Yasir Alsiraj, Xuqi Chen, Sean E. Thatcher, Ryan E. Temel, Lei Cai, Eric M. Blalock, Wendy Katz, Heba M. Ali, Michael C. Petriello, Pan Deng, Andrew J. Morris, Xuping Wang, Aldons J. Lusis, Arthur P. Arnold, Karen Reue, Katherine L. Thompson, Patrick Tso, Lisa A. Cassis

Pharmacology and Nutritional Sciences Faculty Publications

Men and women differ in circulating lipids and coronary artery disease (CAD). While sex hormones such as estrogens decrease CAD risk, hormone replacement therapy increases risk. Biological sex is determined by sex hormones and chromosomes, but effects of sex chromosomes on circulating lipids and atherosclerosis are unknown. Here, we use mouse models to separate effects of sex chromosomes and hormones on atherosclerosis, circulating lipids and intestinal fat metabolism. We assess atherosclerosis in multiple models and experimental paradigms that distinguish effects of sex chromosomes, and male or female gonads. Pro-atherogenic lipids and atherosclerosis are greater in XX than XY mice, indicating ...


An Integrative Cross-Omics Analysis Of Dna Methylation Sites Of Glucose And Insulin Homeostasis, Jun Liu, Elena Carnero-Montoro, Jenny Van Dongen, Samantha Lent, Ivana Nedeljkovic, Symen Ligthart, Pei-Chien Tsai, Tiphaine C. Martin, Pooja R. Mandaviya, Rick Jansen, Marjolein J. Peters, Liesbeth Duijts, Vincent W. V. Jaddoe, Henning Tiemeier, Janine F. Felix, Gonneke Willemsen, Eco J. C. De Geus, Audrey Y. Chu, Daniel Levy, Shih-Jen Hwang, Jan Bressler, Rahul Gondalia, Elias L. Salfati, Christian Herder, Bertha A. Hidalgo, Toshiko Tanaka, Ann Zenobia Moore, Rozenn N. Lemaitre, Min A. Jhun, Jennifer A. Smith, Donna K. Arnett Jun 2019

An Integrative Cross-Omics Analysis Of Dna Methylation Sites Of Glucose And Insulin Homeostasis, Jun Liu, Elena Carnero-Montoro, Jenny Van Dongen, Samantha Lent, Ivana Nedeljkovic, Symen Ligthart, Pei-Chien Tsai, Tiphaine C. Martin, Pooja R. Mandaviya, Rick Jansen, Marjolein J. Peters, Liesbeth Duijts, Vincent W. V. Jaddoe, Henning Tiemeier, Janine F. Felix, Gonneke Willemsen, Eco J. C. De Geus, Audrey Y. Chu, Daniel Levy, Shih-Jen Hwang, Jan Bressler, Rahul Gondalia, Elias L. Salfati, Christian Herder, Bertha A. Hidalgo, Toshiko Tanaka, Ann Zenobia Moore, Rozenn N. Lemaitre, Min A. Jhun, Jennifer A. Smith, Donna K. Arnett

Epidemiology Faculty Publications

Despite existing reports on differential DNA methylation in type 2 diabetes (T2D) and obesity, our understanding of its functional relevance remains limited. Here we show the effect of differential methylation in the early phases of T2D pathology by a blood-based epigenome-wide association study of 4808 non-diabetic Europeans in the discovery phase and 11,750 individuals in the replication. We identify CpGs in LETM1, RBM20, IRS2, MAN2A2 and the 1q25.3 region associated with fasting insulin, and in FCRL6, SLAMF1, APOBEC3H and the 15q26.1 region with fasting glucose. In silico cross-omics analyses highlight the role of differential methylation in the ...


Caloric Restriction Alters Postprandial Responses Of Essential Brain Metabolites In Young Adult Mice, Lucille M. Yanckello, Lyndsay E. A. Young, Jared D. Hoffman, Robert P. Mohney, Mignon A. Keaton, Erin L. Abner, Ai-Ling Lin Jun 2019

Caloric Restriction Alters Postprandial Responses Of Essential Brain Metabolites In Young Adult Mice, Lucille M. Yanckello, Lyndsay E. A. Young, Jared D. Hoffman, Robert P. Mohney, Mignon A. Keaton, Erin L. Abner, Ai-Ling Lin

Sanders-Brown Center on Aging Faculty Publications

Caloric restriction (CR) has been shown to extend longevity and protect brain function in aging. However, the effects of CR in young adult mice remain largely unexplored. In addition to the fundamental, long-term changes, recent studies demonstrate that CR has a significant impact on transient, postprandial metabolic flexibility and turnover compared to control groups. The goal of this study was to identify the brain metabolic changes at a transient (2 h) and steady (6 h) postprandial state in young mice (5–6 months of age) fed with CR or ad libitum (AL; free eating). Using metabolomics profiling, we show that ...


Downregulation Of Orco And 5-Htt Alters Nestmate Discrimination In The Subterranean Termite Ondontotermes Formosanus (Shiraki), Pengdong Sun, Shuxin Yu, Austin Merchant, Chaoliang Lei, Xuguo Zhou, Qiuying Huang Jun 2019

Downregulation Of Orco And 5-Htt Alters Nestmate Discrimination In The Subterranean Termite Ondontotermes Formosanus (Shiraki), Pengdong Sun, Shuxin Yu, Austin Merchant, Chaoliang Lei, Xuguo Zhou, Qiuying Huang

Entomology Faculty Publications

Nestmate discrimination allows social insects to recognize nestmates from non-nestmates using colony-specific chemosensory cues, which typically evoke aggressive behavior toward non-nestmates. Functional analysis of genes associated with nestmate discrimination has been primarily focused on inter-colonial discrimination in Hymenopterans, and parallel studies in termites, however, are grossly lacking. To fill this gap, we investigated the role of two genes, Orco and 5-HTT, associated with chemosensation and neurotransmission respectively, in nestmate discrimination in a highly eusocial subterranean termite, Odontotermes formosanus (Shiraki). We hypothesized that knocking down of these genes will compromise the nestmate recognition and lead to the antagonistic behavior. To test ...


Crystal Structure Of Zymonic Acid And A Redetermination Of Its Precursor, Pyruvic Acid, Dominik Heger, Alexis J. Eugene, Sean R. Parkin, Marcelo I. Guzman Jun 2019

Crystal Structure Of Zymonic Acid And A Redetermination Of Its Precursor, Pyruvic Acid, Dominik Heger, Alexis J. Eugene, Sean R. Parkin, Marcelo I. Guzman

Chemistry Faculty Publications

The structure of zymonic acid (systematic name: 4-hy­droxy-2-methyl-5-oxo-2,5-di­hydro­furan-2-carb­oxy­lic acid), C6H6O5, which had previously eluded crystallographic determination, is presented here for the first time. It forms by intra­molecular condensation of parapyruvic acid, which is the product of aldol condensation of pyruvic acid. A redetermination of the crystal structure of pyruvic acid (systematic name: 2-oxo­propanoic acid), C3H4O3, at low temperature (90 K) and with increased precision, is also presented [for the previous structure, see: Harata et al. (1977). Acta Cryst. B33, 210–212 ...


A Multi-Institutional Analysis Of Instructional Beliefs And Practices In Gateway Courses To The Sciences, Joseph J. Ferrare Jun 2019

A Multi-Institutional Analysis Of Instructional Beliefs And Practices In Gateway Courses To The Sciences, Joseph J. Ferrare

Educational Policy Studies and Evaluation Faculty Publications

This paper builds on previous studies of instructional practice in science, technology, engineering, and mathematics courses by reporting findings from a study of the relationship between instructors’ beliefs about teaching and learning and their observed classroom practices. Data collection took place across six institutions of higher education and included in-depth interviews with 71 instructors and more than 140 hours of classroom observations using the Teaching Dimensions Observation Protocol. Thematic coding of interviews identified 31 distinct beliefs that instructors held about the ways students best learn introductory concepts and skills in these courses. Cluster analysis of the observation data suggested that ...


Forage News [2019-06], University Of Kentucky Department Of Plant And Soil Sciences Jun 2019

Hdl Subclass Proteomic Analysis And Functional Implication Of Protein Dynamic Change During Hdl Maturation, Yuling Zhang, Scott M. Gordon, Hang Xi, Seungbum Choi, Merlin Abner Paz, Runlu Sun, William Yang, Jason Saredy, Mohsin Khan, Alan Thomas Remaley, Jing-Feng Wang, Xiaofeng Yang, Hong Wang Jun 2019

Hdl Subclass Proteomic Analysis And Functional Implication Of Protein Dynamic Change During Hdl Maturation, Yuling Zhang, Scott M. Gordon, Hang Xi, Seungbum Choi, Merlin Abner Paz, Runlu Sun, William Yang, Jason Saredy, Mohsin Khan, Alan Thomas Remaley, Jing-Feng Wang, Xiaofeng Yang, Hong Wang

Saha Cardiovascular Research Center Faculty Publications

Recent clinical trials reported that increasing high-density lipoprotein-cholesterol (HDL-C) levels does not improve cardiovascular outcomes. We hypothesize that HDL proteome dynamics determine HDL cardioprotective functions. In this study, we characterized proteome profiles in HDL subclasses and established their functional connection. Mouse plasma was fractionized by fast protein liquid chromatography, examined for protein, cholesterial, phospholipid and trigliceride content. Small, medium and large (S/M/L)-HDL subclasseses were collected for proteomic analysis by mass spectrometry. Fifty-one HDL proteins (39 in S-HDL, 27 in M-HDL and 29 in L-HDL) were identified and grouped into 4 functional categories (lipid metabolism, immune response, coagulation ...


Opportunities For Self-Evaluation Increase Student Calibration In An Introductory Biology Course, Jennifer L. Osterhage, Ellen Usher, Trisha A. Douin, William M. Bailey Jun 2019

Opportunities For Self-Evaluation Increase Student Calibration In An Introductory Biology Course, Jennifer L. Osterhage, Ellen Usher, Trisha A. Douin, William M. Bailey

Biology Faculty Publications

Accurate self-evaluation is critical for learning. Calibration describes the relationship between learners’ perception of their performance and their actual performance on a task. Here, we describe two studies aimed at assessing and improving student calibration in a first-semester introductory biology course at a 4-year public institution. Study 1 investigated students’ (n = 310) calibration (the difference between estimated and actual exam performance) across one semester. Students were significantly miscalibrated for the first exam: their predicted scores were, on average, significantly higher than their actual scores. The lowest-performing students had the most inaccurate estimates. Calibration improved with each exam. By the final ...


Protective Effects Of Novel Derivatives Of Vitamin D3 And Lumisterol Against Uvb-Induced Damage In Human Keratinocytes Involve Activation Of Nrf2 And P53 Defense Mechanisms, Anyamanee Chaiprasongsuk, Zorica Janjetovic, Tae-Kang Kim, Stuart G. Jarrett, John A. D'Orazio, Michael F. Holick, Edith K. Y. Tang, Robert C. Tuckey, Uraiwan Panich, Wei Li, Andrzej T. Slominski Jun 2019

Protective Effects Of Novel Derivatives Of Vitamin D3 And Lumisterol Against Uvb-Induced Damage In Human Keratinocytes Involve Activation Of Nrf2 And P53 Defense Mechanisms, Anyamanee Chaiprasongsuk, Zorica Janjetovic, Tae-Kang Kim, Stuart G. Jarrett, John A. D'Orazio, Michael F. Holick, Edith K. Y. Tang, Robert C. Tuckey, Uraiwan Panich, Wei Li, Andrzej T. Slominski

Toxicology and Cancer Biology Faculty Publications

We tested whether novel CYP11A1-derived vitamin D3- and lumisterol-hydroxyderivatives, including 1,25(OH)2D3, 20(OH)D3, 1,20(OH)2D3, 20,23(OH)2D3, 1,20,23(OH)3D3, lumisterol, 20(OH)L3, 22(OH)L3, 20,22(OH)2L3, and 24(OH)L3, can protect against UVB-induced damage in human epidermal keratinocytes. Cells were treated with above compounds for 24 h, then subjected to UVB irradiation at UVB doses of 25, 50, 75, or 200 mJ/cm2, and then examined for oxidant ...


Validation Of A Commercial Automated Body Condition Scoring System On A Commercial Dairy Farm, Israel L. Mullins, Carissa M. Truman, Magnus R. Campler, Jeffrey M. Bewley, Joao H. C. Costa May 2019

Validation Of A Commercial Automated Body Condition Scoring System On A Commercial Dairy Farm, Israel L. Mullins, Carissa M. Truman, Magnus R. Campler, Jeffrey M. Bewley, Joao H. C. Costa

Animal and Food Sciences Faculty Publications

Body condition scoring (BCS) is the management practice of assessing body reserves of individual animals by visual or tactile estimation of subcutaneous fat and muscle. Both high and low BCS can negatively impact milk production, disease, and reproduction. Visual or tactile estimation of subcutaneous fat reserves in dairy cattle relies on their body shape or thickness of fat layers and muscle on key areas of the body. Although manual BCS has proven beneficial, consistent qualitative scoring can be difficult to implement. The desirable BCS range for dairy cows varies within lactation and should be monitored at multiple time points throughout ...


Stimulatory Effect Of 5-Hydroxytryptamine (5-Ht) On Rat Capsaicin-Sensitive Lung Vagal Sensory Neurons Via Activation Of 5-Ht3 Receptors, Chun-Chun Hsu, Ting Ruan, Lu-Yuan Lee, You Shuei Lin May 2019

Stimulatory Effect Of 5-Hydroxytryptamine (5-Ht) On Rat Capsaicin-Sensitive Lung Vagal Sensory Neurons Via Activation Of 5-Ht3 Receptors, Chun-Chun Hsu, Ting Ruan, Lu-Yuan Lee, You Shuei Lin

Physiology Faculty Publications

5-hydroxytryptamine (5-HT) is an inflammatory mediator known to be released in lung. Capsaicin-sensitive lung vagal (CSLV) afferents function as a primary sensor for detecting chemical stimuli and produce consequent reflexes during lung inflammation. To characterize the effect of 5-HT on CSLV afferents, responses of cardiorespiratory reflexes and single-unit C-fiber afferents to right-atrial injections of 5-HT were investigated in anesthetized Sprague-Dawley rats. Bolus injection of 5-HT (8 μg/kg) caused an immediate augmented breath and apnea, accompanied by hypotension and bradycardia. These initial responses were then followed by a brief pressor response and a more sustained depressor response. After a perineural ...


Rediscovery Of The Endangered Carchi Andean Toad, Rhaebo Colomai (Hoogmoed, 1985), In Ecuador, With Comments On Its Conservation Status And Extinction Risk, Carolina Reyes-Puig, Gabriela B. Bittencourt-Silva, María Torres-Sánchez, Mark Wilkinson, Jeffrey W. Streicher, Simon T. Maddock, Ramachandran Kotharambath, Hendrik Müller, Francesca Nicole Angiolani Larrera, Diego Amieda-Reinoso, Santiago R. Ron, Diego Francisco Cisneros-Heredia May 2019

Rediscovery Of The Endangered Carchi Andean Toad, Rhaebo Colomai (Hoogmoed, 1985), In Ecuador, With Comments On Its Conservation Status And Extinction Risk, Carolina Reyes-Puig, Gabriela B. Bittencourt-Silva, María Torres-Sánchez, Mark Wilkinson, Jeffrey W. Streicher, Simon T. Maddock, Ramachandran Kotharambath, Hendrik Müller, Francesca Nicole Angiolani Larrera, Diego Amieda-Reinoso, Santiago R. Ron, Diego Francisco Cisneros-Heredia

Neuroscience Faculty Publications

Since 1984 there have been no records of Rhaebo colomai (Hoogmoed, 1985) within the territory of Ecuador. This species was known from 2 localities in the province of Carchi, northwestern Ecuador, and the department of Nariño, southwestern Colombia, which were reported in 1979 and 2015, respectively. We report the recent sightings of R. colomai at 3 new localities in Ecuador and discuss and evaluate this species’ extinction risk and conservation status.


Root Hair Single Cell Type Specific Profiles Of Gene Expression And Alternative Polyadenylation Under Cadmium Stress, Jingyi Cao, Congting Ye, Guijie Hao, Carole Dabney-Smith, Arthur G. Hunt, Qingshun Q. Li May 2019

Root Hair Single Cell Type Specific Profiles Of Gene Expression And Alternative Polyadenylation Under Cadmium Stress, Jingyi Cao, Congting Ye, Guijie Hao, Carole Dabney-Smith, Arthur G. Hunt, Qingshun Q. Li

Plant and Soil Sciences Faculty Publications

Transcriptional networks are tightly controlled in plant development and stress responses. Alternative polyadenylation (APA) has been found to regulate gene expression under abiotic stress by increasing the heterogeneity at mRNA 3′-ends. Heavy metals like cadmium pollute water and soil due to mining and industry applications. Understanding how plants cope with heavy metal stress remains an interesting question. The Arabidopsis root hair was chosen as a single cell model to investigate the functional role of APA in cadmium stress response. Primary root growth inhibition and defective root hair morphotypes were observed. Poly(A) tag (PAT) libraries from single cell types ...


Facilitation Of Neuropathic Pain By The Npy Y1 Receptor-Expressing Subpopulation Of Excitatory Interneurons In The Dorsal Horn, Tyler S. Nelson, Weisi Fu, Renee R. Donahue, Gregory F. Corder, Tomas Hökfelt, Ronald G. Wiley, Bradley K. Taylor May 2019

Facilitation Of Neuropathic Pain By The Npy Y1 Receptor-Expressing Subpopulation Of Excitatory Interneurons In The Dorsal Horn, Tyler S. Nelson, Weisi Fu, Renee R. Donahue, Gregory F. Corder, Tomas Hökfelt, Ronald G. Wiley, Bradley K. Taylor

Physiology Faculty Publications

Endogenous neuropeptide Y (NPY) exerts long-lasting spinal inhibitory control of neuropathic pain, but its mechanism of action is complicated by the expression of its receptors at multiple sites in the dorsal horn: NPY Y1 receptors (Y1Rs) on post-synaptic neurons and both Y1Rs and Y2Rs at the central terminals of primary afferents. We found that Y1R-expressing spinal neurons contain multiple markers of excitatory but not inhibitory interneurons in the rat superficial dorsal horn. To test the relevance of this spinal population to the development and/or maintenance of acute and neuropathic pain, we selectively ablated Y1R-expressing interneurons with intrathecal administration of ...