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Cloning And Expression Analysis Of Drosophila Extracellular Cu Zn Superoxide Dismutase, Michael J. Blackney, Rebecca Cox, David Shepherd, Joel D. Parker Dec 2014

Cloning And Expression Analysis Of Drosophila Extracellular Cu Zn Superoxide Dismutase, Michael J. Blackney, Rebecca Cox, David Shepherd, Joel D. Parker

Joel D Parker

In the present study, we cloned and sequenced the mRNAs of the Sod3 [extracellular Cu Zn SOD (superoxide dismutase)] gene in Drosophila and identified two mRNA products formed by alternative splicing. These products code for a long and short protein derived from the four transcripts found in global expression studies (Flybase numbers Dmel\CG9027, FBgn0033631). Both mRNA process variants contain an extracellular signalling sequence, a region of high homology to the Sod1 (cytoplasmic Cu Zn SOD) including a conserved AUG start, with the longer form also containing a hydrophobic tail. The two fully processed transcripts are homologous to Caenorhabditis elegans ...