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2001

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Articles 1 - 22 of 22

Full-Text Articles in Life Sciences

Use Of Molecular Probes To Assess Geographic Distribution Of Pfiesteria Species, Torstein Tengs Oct 2001

Use Of Molecular Probes To Assess Geographic Distribution Of Pfiesteria Species, Torstein Tengs

Dr. Torstein Tengs

We have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp. in cultures and environmental samples. More than 2,100 water and sediment samples from estuarine sites of the U.S. Atlantic and gulf coasts were assayed for the presence of Pfiesteria piscicida Steidinger & Burkholder and Pfiesteria shumwayae Glasgow & Burkholder by PCR probing of extracted DNA. Positive results were found in about 3% of samples derived from routine monitoring of coastal waters and about 8% of sediments. The geographic range of both species was the same, ranging from New York to Texas. Pfiesteria spp. are ...


Classification And Identification Of Pfiesteria And Pfiesteria-Like Species, Torstein Tengs Oct 2001

Classification And Identification Of Pfiesteria And Pfiesteria-Like Species, Torstein Tengs

Dr. Torstein Tengs

Dinoflagellates can be classified both botanically and zoologically; however, they are typically put in the botanical division Pyrrhophyta. As a group they appear most related to the protistan ciliates and apicomplexans at the ultrastructure level. Within the Pyrrhophyta are both unarmored and armored forms of the dominant, motile flagellated stage. Unarmored dinoflagellates do not have thecal or wall plates arranged in specific series, whereas armored species have plates that vary in thickness but are specific in number and arrangement. In armored dinoflagellates, the plate pattern and tabulation is a diagnostic character at the family, subfamily, and even genus levels. In ...


Influential Parameters Of Job Stress - A Regression Analysis Of Women Scientists In Indian Agricultural Research System, Aneeja Guttikonda Aug 2001

Influential Parameters Of Job Stress - A Regression Analysis Of Women Scientists In Indian Agricultural Research System, Aneeja Guttikonda

aneeja guttikonda

No abstract provided.


Β-Lactoglobulin Molten Globule Induced By High Pressure, Jian Yang, A. Keith Dunker, Joseph R. Powers, Stephanie Clark, Barry G. Swanson Jun 2001

Β-Lactoglobulin Molten Globule Induced By High Pressure, Jian Yang, A. Keith Dunker, Joseph R. Powers, Stephanie Clark, Barry G. Swanson

Stephanie Clark

Beta-lactoglobulin (beta-LG) was treated with high hydrostatic pressure (HHP) at 600 MPa and 50 degrees C for selected times as long as 64 min. The intrinsic tryptophan fluorescence of beta-LG indicated that HHP treatment conditions induced a conformational change. HHP treatment conditions also promote a 3-fold increase in the extrinsic fluorescence of 1-anilinonaphthalene-8-sulfonate and a 2.6-fold decrease for cis-paraneric acid, suggesting an increase in accessible aromatic hydrophobicity and a decrease in aliphatic hydrophobicity. Far-ultraviolet circular dichroism (CD) spectra reveal that the secondary structure of beta-LG converts from native beta-sheets to non-native alpha-helices following HHP treatment, whereas near-ultraviolet CD spectra ...


Visualizing Rhizosphere Chemistry Of Legumes With Mid-Infrared Synchrotron Radiation, Ted K. Raab, Michael C. Martin May 2001

Visualizing Rhizosphere Chemistry Of Legumes With Mid-Infrared Synchrotron Radiation, Ted K. Raab, Michael C. Martin

Ted K. Raab

A bright synchrotron light source operated by the Lawrence Berkeley National Laboratory served as an external source for infrared (IR) microscopy of plant root microcosms. Mid-IR light from synchrotrons is 2-3 orders of magnitude brighter than conventional sources, providing contrast based on the chemical information in the reflected signal at a spatial resolution near the diffraction-limit of 3-10 microns. In an experiment using plant root microcosms fitted with zinc selenide IR-transmissive windows (50 mm x 20 mm x 1 mm), we describe chemical differences and similarities within the root zone of mung bean (Vigna radiata L.), grown with or without ...


Method For Determining Oxidation Of Vegetable Oils By Near-Infrared Spectroscopy, Gulgun (Yildiz) Tiryaki May 2001

Method For Determining Oxidation Of Vegetable Oils By Near-Infrared Spectroscopy, Gulgun (Yildiz) Tiryaki

Dr. Gülgün YILDIZ TIRYAKI

No abstract provided.


Genetic Polymorphism In Gymnodinium Galatheanum Chloroplast Dna Sequences And Development Of A Molecular Detection Assay, Torstein Tengs Feb 2001

Genetic Polymorphism In Gymnodinium Galatheanum Chloroplast Dna Sequences And Development Of A Molecular Detection Assay, Torstein Tengs

Dr. Torstein Tengs

Nuclear and chloroplast-encoded small subunit ribosomal DNA sequences were obtained from several strains of the toxic dinoflagellate Gymnodinium galatheanum . Phylogenetic analyses and comparison of sequences indicate that the chloroplast sequences show a higher degree of sequence divergence than the nuclear homologue. The chloroplast sequences were chosen as targets for the development of a 5'–3' exonuclease assay for detection of the organism. The assay has a very high degree of specificity and has been used to screen environmental water samples from a fish farm where the presence of this dinoflagellate species has previously been associated with fish kills. Various hypotheses ...


N-Methyl-D-Aspartate Channel And Consciousness: From Signal Coincidence Detection To Quantum Computing, Armando F. Rocha, Alfredo Pereira Jr Jan 2001

N-Methyl-D-Aspartate Channel And Consciousness: From Signal Coincidence Detection To Quantum Computing, Armando F. Rocha, Alfredo Pereira Jr

Armando F Rocha

Research on Blindsight, Neglect/Extinction and Phantom limb syndromes, as well as electrical measurements of mammalian brain activity, have suggested the dependence of vivid perception on both incoming sensory information at primary sensory cortex and reentrant information from associative cortex. Coherence between incoming and reentrant signals seems to be a necessary condition for (conscious) perception. General reticular activating system and local electrical synchronization are some of the tools used by the brain to establish coarse coherence at the sensory cortex, upon which biochemical processes are coordinated. Besides electrical synchrony and chemical modulation at the synapse, a central mechanism supporting such ...


Ecocomposite Materials, David A. Bainbridge Jan 2001

Ecocomposite Materials, David A. Bainbridge

David A Bainbridge

Ecocomposite materials combine fibers, resins and natural materials for added strength and durability. These materials can be fully and easily recycled by nature.


Nitrogen Pollution, David A. Bainbridge Jan 2001

Nitrogen Pollution, David A. Bainbridge

David A Bainbridge

Nitrogen pollution is an severe problem in many ecosystems. It is rarely recognized or appreciated on land because the effects can be slow -- but they can be catastrophic. Native species are driven out by high response weeds and invasive species that may totally disrupt ecosystem structure and function.


Real–Time Pcr Monitoring Of Estuarine Water Samples For Pfiesteria Piscicida, A Dinoflagellate Associated With Fish Kills And Human Illness, Torstein Tengs Jan 2001

Real–Time Pcr Monitoring Of Estuarine Water Samples For Pfiesteria Piscicida, A Dinoflagellate Associated With Fish Kills And Human Illness, Torstein Tengs

Dr. Torstein Tengs

No abstract.


Teaching The Human Dimensions Of Care In The Clinical Settings, William T. Branch Jr. Md, Macp Jan 2001

Teaching The Human Dimensions Of Care In The Clinical Settings, William T. Branch Jr. Md, Macp

William T.Branch Jr.MD

Despite repeated calls to emphasize the humanistic dimensions of care during medical education, these are few known techniques for effective teaching of humanism. We describe the barriers that inhibit humanistic teaching and suggest pragmatic teaching methods to overcome such barriers and teach humanistic care in clinical settings. We began by asking participants at a conference on patient-physician communications sponsored by the American Academy on Physician and Patient in June 1998, "What can we do in the patient's presence to improve and teach the human dimensions of care? Please provide one or more examples of approaches you found to be ...


The Use Of Red Mangrove As Nesting Substrate For Snail Kites In Florida, Victoria Dreitz, James Duberstein Jan 2001

The Use Of Red Mangrove As Nesting Substrate For Snail Kites In Florida, Victoria Dreitz, James Duberstein

Victoria Dreitz

No abstract provided.


Population Viability Of The Gulf Of Mexico Sturgeon In The Suwannee River, Florida, William Pine, Michael Allen, Victoria Dreitz Jan 2001

Population Viability Of The Gulf Of Mexico Sturgeon In The Suwannee River, Florida, William Pine, Michael Allen, Victoria Dreitz

Victoria Dreitz

No abstract provided.


Vasorelaxant Effects Of Ethyl Cinnamate Isolated From Kaempferia Galanga On Smooth Muscles Of The Rat Aorta, Mohd Rais Mustafa, Rozana Othman, Halijah Ibrahim, Mustafa Ali Mohd, Khalijah Awang, Anwar-Ul Hassan Gilani Jan 2001

Vasorelaxant Effects Of Ethyl Cinnamate Isolated From Kaempferia Galanga On Smooth Muscles Of The Rat Aorta, Mohd Rais Mustafa, Rozana Othman, Halijah Ibrahim, Mustafa Ali Mohd, Khalijah Awang, Anwar-Ul Hassan Gilani

Mohd Rais Mustafa

From the rhizomes of Kaempferia galanga, ethyl cinnamate (EC) was isolated and its vasorelaxant effect was examined on the rat aorta. EC inhibited the tonic contractions induced by high K+ and phenylephrine (PE) in a concentration-dependent manner, with respective IC50 values of 0.30 ± 0.05 mM and 0.38 ± 0.04 mM. The relaxant effect against PE-induced contractions was greater in the presence of endothelium. Pre-treatment of the aorta with methylene blue and indomethacin significantly reduced the relaxant effect. These results suggest that the inhibitory effects of EC may involve inhibition of Ca2+ influx into vascular cells and release ...


The Cognitive And Motivational Effects Of Imagery Training: A Matter Of Perspective, Jennifer L. Cumming, Diane M. Ste-Marie Jan 2001

The Cognitive And Motivational Effects Of Imagery Training: A Matter Of Perspective, Jennifer L. Cumming, Diane M. Ste-Marie

Jennifer Cumming

No abstract provided.


El Laboratorio De Cádiz Del Instituto De Investigaciones Pesqueras : ”Arena” Investigadora Del Csic Entre 1957 Y 1966., Enrique Wulff Jan 2001

El Laboratorio De Cádiz Del Instituto De Investigaciones Pesqueras : ”Arena” Investigadora Del Csic Entre 1957 Y 1966., Enrique Wulff

Enrique Wulff

The aim of this communication is to detail some main images projected by CSIC from its laboratuory in Cádiz, as critical information points from the past. 1957-1966 is the studied period, its fondational time. The indexation of the scientific problems related with the activity of the laboratory makes use of the calculation of transition probabilities. Catch percentages and bathymetric distributions are emoployed to approach the structure of the oceanographic campaigns results. The quantitative enumeration of observed organs and mathematical procedures provide information on the observations in the tunny fisheries. Multivariant bibliometry (factorial and cluster analysis, multiple regression) , and coauthor and ...


The Ethics Of Sport: A Reader, Chad R. Carlson Jan 2001

The Ethics Of Sport: A Reader, Chad R. Carlson

Chad Carlson

No abstract provided.


Genetic Heterogeneity Of White-Tailed Deer: Management Lessons From A Long-Term Study, Michael H. Smith, James M. Novak, J. D. Peles, J. R. Purdue Jan 2001

Genetic Heterogeneity Of White-Tailed Deer: Management Lessons From A Long-Term Study, Michael H. Smith, James M. Novak, J. D. Peles, J. R. Purdue

James M. Novak

Genetic data from a long-term (16-year) study of white-tailed deer (Odocoileus virginianus) on the U.S. Department of Energy's Savannah River Site (SRS) were examined to evaluate spatial and temporal genetic heterogeneity in this species. Based on our analyses of the long-term data set, three major findings emerged, all of which have important implications for management of white-tailed deer: (1) There exists significant spatial genetic heterogeneity in white-tailed deer based on analyses of allozyme frequencies and mtDNA haplotypes. This heterogeneity exists on a much smaller spatial scale than would be expected for such a large and potentially mobile species ...


Phylogeographic Analysis Of The Threatened And Endangered Superconglutinate-Producing Mussels Of The Genus Lampsilis (Bivalvia: Unionidae), Kevin J. Roe, Charles Lydeard, Paul D. Hartfield Jan 2001

Phylogeographic Analysis Of The Threatened And Endangered Superconglutinate-Producing Mussels Of The Genus Lampsilis (Bivalvia: Unionidae), Kevin J. Roe, Charles Lydeard, Paul D. Hartfield

Kevin J. Roe

Several species of freshwater unionid mussels in the genus Lampsilis exhibit a remarkable reproductive strategy. Female mussels of these species enclose their larvae in a minnow-like lure, called a ‘superconglutinate’, to attract piscivorous fishes. When a fish attempts to ingest the superconglutinate the lure ruptures and the larvae are released to parasitize the fish. Of the four species of mussel which exhibit this strategy and are endemic to the Gulf Coast drainages of the southeastern United States, three are protected under the Endangered Species Act, and one is recognized as imperilled. Phylogenetic analysis of nucleotide sequences of the mitochondrial 16S ...


The Unusual Life History Of Alseis Blackiana: A Shade-Persistent Pioneer Tree?, Jason D. Nason, J. W. Dalling, K. Winter, S. P. Hubbell, D. A. Murawski, J. L. Hamrick Jan 2001

The Unusual Life History Of Alseis Blackiana: A Shade-Persistent Pioneer Tree?, Jason D. Nason, J. W. Dalling, K. Winter, S. P. Hubbell, D. A. Murawski, J. L. Hamrick

Jason D Nason

Classifications of tree species into regeneration guilds or functional groups are often based on a limited number of life history characteristics indicative of requirements for early establishment, with the assumption that these traits are correlated with growth requirements of larger individuals. We tested the validity of this assumption for Alseis blackiana, a common canopy tree in central Panama, by examining its seed germination, seedling growth, and sapling distribution, growth, and mortality. We found that the early life history of Alseis is characteristic of pioneer species: the seed germination rate was much higher in forest gaps than in the forest understory ...


Effects Of Elevated Co2 On The Response Of Phleum Pratense And Poa Pratensis To Aboveground Defoliation And Root-Feeding Nematodes, Brian J. Wilsey Jan 2001

Effects Of Elevated Co2 On The Response Of Phleum Pratense And Poa Pratensis To Aboveground Defoliation And Root-Feeding Nematodes, Brian J. Wilsey

Brian J. Wilsey

Because of the importance of herbivory in many herbaceous plant–dominated ecosystems, it will be important to determine whether interactions exist between herbivory and CO2 enrichment if we are to understand better how ecosystems will respond to global change. In an experiment with the C3 grasses Poa pratensis and Phleum pratense, plants were grown together under ambient (360 ppm) and elevated (650 ppm) CO2, with and without aboveground defoliation, and with or without additions of the root-feeding nematode Pratylenchus penetrans. The objective was to determine whether herbivory would interact with CO2 enrichment in determining biomass responses or whether these factors ...