Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 14 of 14

Full-Text Articles in Life Sciences

Progress And Future Prospects Of Developing Transgenics In Mango, Rajesh Pati Oct 2013

Progress And Future Prospects Of Developing Transgenics In Mango, Rajesh Pati

Rajesh Pati

Mango (Mangifera indica L.) is the most important fruit crop of the world. India ranks first among world’s mango producing countries accounting for 57.18 % of the total world mango production of 19.22 million tones. With the development of rDNA technology, plant breeders have access to a large number of genes that can be integrated into the plant genome.


Histological And Biochemical Changes In Aegle Marmelos, Rajesh Pati Oct 2013

Histological And Biochemical Changes In Aegle Marmelos, Rajesh Pati

Rajesh Pati

Aegle marmelos Corr., belongs to family Rutaceae, is more prized for its pharmacological virtues than its edible quality. Because of pharmacological importance, it’s become potential candidate for developing transgenics to enhance its medicinal properties. Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (In vitro stage, acclimation stage, and field established plants).


Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati Oct 2013

Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati

Rajesh Pati

A standard uniform protocol of regeneration is the prime and foremost prerequisite for not only improve the productivity, the production from the existing area but also development of transgenics in mango for various traits. Polyembryonic cultivars are good rootstock because of moderate canopy, tolerant to draught, salt, environmental stress (wind, high temperature, air pollution), fungal disease (anthracnose, powdery mildew, gummosis) and hoppers.


Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati Oct 2013

Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati

Rajesh Pati

Aegle marmelos Corr. is better known for its medicinal values rather than its edible quality. Every part of the plant viz. leaves, fruits, roots, and the bark are used for treating various diseases related to heart, stomach, bacterial, fungal, viral, worms, fertility, fever and cosmetics. Marmelosin is one of the major compounds (coumarin), used to treat various diseases. Till date, there are no varieties developed for commercial orchards of Bael and hence, regarded as one among the underutilized fruits of India. Therefore, there is an urgent need for breeding new and cultivable varieties in Aegle marmelos. But the problem for ...


Micropropagation In Fruit Crops, Rajesh Pati Oct 2013

Micropropagation In Fruit Crops, Rajesh Pati

Rajesh Pati

Micropropagation is a proven means of producing millions of identical plants under a controlled and aseptic condition, independent of seasonal constraints. It not only provides economy of time and space but also gives greater output and allows further augmentation of elite disease free propagules. India is homeland of many important fruit crops such as Indian gooseberry (Emblica officinalis Gaertn), bael (Aegle marmelos Corr.), Guava (, Psidium guajava), jamun or black plum (Syzygium cuminii L. Skeels.), Mango (Mangifera indica) and Papaya (Carica papaya).


In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati Oct 2013

In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati

Rajesh Pati

Bael (Aegle marmelos Corr.) is an important medicinal fruit tree. The fruit pulp contains marmelosin, which is a laxative, diuretic, is being used in many patented drugs in India. Micropropagation technology can be gainfully employed in mass multiplication of improved bael varieties. We have developed micropropagation protocol of bael through shoot bud culture. It was imperative to test genetic fidelity of micropropagated plants using molecular markers (RAPD, DAMD and ISSR).


Histological And Biochemical Changes In Aegle Marmelos Corr. Before And After Acclimatization, Rajesh Pati Apr 2013

Histological And Biochemical Changes In Aegle Marmelos Corr. Before And After Acclimatization, Rajesh Pati

Rajesh Pati

Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (In vitro stage, acclimation stage). The biochemical result showed that micropropagated plantlets produced significantly low total chlorophyll (0.042 mg/g fresh weight), reducing sugar (3.227%), NR activity (1.353 NO2/h/g fresh weight) and but higher protein (0.048 μg/g) during in vitro phase. The in vitro raised plants showed ...


Micropropagation Of Mangifera Indica L. Cv. Kurakkan Through Somatic Embryogenesis, Rajesh Pati Oct 2010

Micropropagation Of Mangifera Indica L. Cv. Kurakkan Through Somatic Embryogenesis, Rajesh Pati

Rajesh Pati

Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.52μM 2,4- D, 0.05% malt extract and 13.78μM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26μM 2,4-D). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15oC. Somatic ...


Efficacy Of Different Detergents For Leaf Extract Based Sex Diagnostics Of Papaya (Carica Papaya L.)., Rajesh Pati Apr 2010

Efficacy Of Different Detergents For Leaf Extract Based Sex Diagnostics Of Papaya (Carica Papaya L.)., Rajesh Pati

Rajesh Pati

No abstract provided.


Isolation, Purification And Biochemical Characterization Of Lectin From Oyster Mushroom, Pleurotus Sajor-Caju., Rajesh Pati Oct 2009

Isolation, Purification And Biochemical Characterization Of Lectin From Oyster Mushroom, Pleurotus Sajor-Caju., Rajesh Pati

Rajesh Pati

No abstract provided.


In Vitro Regeneration And Genetic Fidelity Testing Of Aegle Marmelos (Corr.) Plants, Rajesh Pati Oct 2008

In Vitro Regeneration And Genetic Fidelity Testing Of Aegle Marmelos (Corr.) Plants, Rajesh Pati

Rajesh Pati

No abstract provided.


In Vitro Plant Regeneration From Mature Explant Of Aegle Marmelos Corr. Cv. Cish-B2, Rajesh Pati Oct 2008

In Vitro Plant Regeneration From Mature Explant Of Aegle Marmelos Corr. Cv. Cish-B2, Rajesh Pati

Rajesh Pati

No abstract provided.


Micropropagation Of Guava (Psidium Guajava L.), Rajesh Pati Oct 2007

Micropropagation Of Guava (Psidium Guajava L.), Rajesh Pati

Rajesh Pati

A foolproof method of micropropagation through in vitro shoot bud culture has been developed in guava cv. ‘Allahabad Safeda’. The stock plant of guava was maintained at shade net house (75% shade) and pruned severely to encourage new growth. Around 3 cm long shoots were excised and defoliated. The explants were pretreated in solution containing 0.1% Carbendazime and 100 mg/L PVP for 1 hour and then washed with Tween-20 wetting agent. The pretreated explants were further treated with HgCl2 0.1% for 5 minutes aseptically followed by six washing in autoclaved distilled water. The sterilized explants were cultured ...


). Clonal Micropropagation Of Indian Gooseberry (Emblica Officinalis Gaertn)., Rajesh Pati Oct 2006

). Clonal Micropropagation Of Indian Gooseberry (Emblica Officinalis Gaertn)., Rajesh Pati

Rajesh Pati

No abstract provided.