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Full-Text Articles in Life Sciences

Characterization Of Bovine-Derived Porous Hydroxyapatite Scaffold And Its Potential To Support Osteogenic Differentiation Of Human Bone Marrow Derived Mesenchymal Stem Cells., Krishnamurithy G, Murali Mr, Hamdi M, Abbas Aa, Raghavendran Hb, Kamarul T Jan 2014

Characterization Of Bovine-Derived Porous Hydroxyapatite Scaffold And Its Potential To Support Osteogenic Differentiation Of Human Bone Marrow Derived Mesenchymal Stem Cells., Krishnamurithy G, Murali Mr, Hamdi M, Abbas Aa, Raghavendran Hb, Kamarul T

Murali Malliga Raman

Porous three-dimensional hydroxyapatite (HA) scaffolds were prepared using bovine cortical bone derived HA (BDHA). Analyses of the morphology, chemical composition, and phase purity of the scaffold were performed using scanning electron microscopy (SEM), micro-computer tomography (micro-CT), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD). SEM images revealed the rough and porous surface of the scaffold, while micro-CT showed the average porous volume of 76.7±0.6% and pore size of 0.04–0.25 mm. Single phase corresponding to standard HA was observed using XRD, and FTIR confirmed the presence of functional groups ...


Histology, Glycosaminoglycan Level And Cartilage Stiffness In Monoiodoacetate-Induced Osteoarthritis: Comparative Analysis With Anterior Cruciate Ligament Transection In Rat Model And Human Osteoarthritis, Naveen Sv, Ahmad Re, Hui Wj, Suhaeb Am, Murali Malliga Raman, Shanmugam R, Kamarul T Jan 2014

Histology, Glycosaminoglycan Level And Cartilage Stiffness In Monoiodoacetate-Induced Osteoarthritis: Comparative Analysis With Anterior Cruciate Ligament Transection In Rat Model And Human Osteoarthritis, Naveen Sv, Ahmad Re, Hui Wj, Suhaeb Am, Murali Malliga Raman, Shanmugam R, Kamarul T

Murali Malliga Raman

Monosodium -iodoacetate (MIA)-induced animal model of osteoarthritis (OA) is under-utilised despite having many inherent advantages. At present, there is lack of studies that directly compare the degenerative changes induced by MIA with the surgical osteoarthritis induction method and human osteoarthritis, which would further verify a greater use of this model. Therefore, we compared the histological, biochemical and biomechanical characteristics in rat model using MIA against the anterior cruciate ligament transection (ACLT) and human cartilage with clinically established osteoarthritis. The right knees of Sprague-Dawley rats were subjected to either MIA or ACLT (n=18 in each group). Six rats were ...


Fluoroscopy Assisted Minimally Invasive Transplantation Of Allogenic Mesenchymal Stromal Cells Embedded In Hystemtm Reduce The Progression Nucleus Pulposus Degeneration In The Damaged Intervertebral Disc - A Preliminary Study In Rabbits, Suhban Ra, Puvanan K, Murali Mr, Raghavendran Hb, Shani S, Abdullah Bj, Abbas Aa, Mohamad Ja, Kamarul T Jan 2014

Fluoroscopy Assisted Minimally Invasive Transplantation Of Allogenic Mesenchymal Stromal Cells Embedded In Hystemtm Reduce The Progression Nucleus Pulposus Degeneration In The Damaged Intervertebral Disc - A Preliminary Study In Rabbits, Suhban Ra, Puvanan K, Murali Mr, Raghavendran Hb, Shani S, Abdullah Bj, Abbas Aa, Mohamad Ja, Kamarul T

Murali Malliga Raman

This study was conducted to develop a technique for minimally invasive and accurate delivery of stem cells to augment nucleus pulposus (NP) in damaged intervertebral discs (IVD). IVD damage was created in noncontiguous discs at L4-L5 level; rabbits (N = 12) were randomly divided into three groups: group I treated with MSCs in HyStem hydrogel, group II treated with HyStem alone, and group III received no intervention. MSCs and hydrogel were administered to the damaged disc under guidance of fluoroscopy. Augmentation of NP was assessed through histological and MRI T2 mapping of the NP after eight weeks of transplantation. T2 weighted ...


Biomechanical Analysis Of The Wrist Arthroplasty In Rheumatoid Arthritis: A Finite Element Analysis, Bajuri Mn, Abdul Kadir Mr, Murali Mr, Kamarul T Jan 2013

Biomechanical Analysis Of The Wrist Arthroplasty In Rheumatoid Arthritis: A Finite Element Analysis, Bajuri Mn, Abdul Kadir Mr, Murali Mr, Kamarul T

Murali Malliga Raman

The total replacement of wrists affected by rheumatoid arthritis (RA) has had mixed outcomes in terms of failure rates. This study was therefore conducted to analyse the biomechanics of wrist arthroplasty using recently reported implants that have shown encouraging results with the aim of providing some insights for the future development of wrist implants. A model of a healthy wrist was developed using computed tomography images from a healthy volunteer. An RA model was simulated based on all ten general characteristics of the disease. The ReMotion ™ total wrist system was then modelled to simulate total wrist arthroplasty (TWA). Finite element ...


A Comparative Study On Morphochemical Properties And Osteogenic Cell Differentiation Within Bone Graft And Coral Graft Culture Systems., Puvaneswary S, Balaji Raghavendran Hr, Ibrahim Ns, Murali Mr, Merican Am, Kamarul T Jan 2013

A Comparative Study On Morphochemical Properties And Osteogenic Cell Differentiation Within Bone Graft And Coral Graft Culture Systems., Puvaneswary S, Balaji Raghavendran Hr, Ibrahim Ns, Murali Mr, Merican Am, Kamarul T

Murali Malliga Raman

The objective of this study was to compare the morphological and chemical composition of bone graft (BG) and coral graft (CG) as well as their osteogenic differentiation potential using rabbit mesenchymal stem cells (rMSCs) in vitro. SEM analysis of BG and CG revealed that the pores in these grafts were interconnected, and their micro-CT confirmed pore sizes in the range of 107-315 µm and 103-514 µm with a total porosity of 92% and 94%, respectively. EDS analysis indicated that the level of calcium in CG was relatively higher than that in BG. FTIR of BG and CG confirmed the presence ...


Characterization And Antibacterial Properties Of Stable Silver Substituted Hydroxyapatite Nanoparticles Synthesized Through Surfactant Assisted Microwave Process., Iqbal N, Abdul Kadir Mr, Nik Malekb Nan, Mahmooda Nhb, Murali Mr, Kamarul T Jan 2013

Characterization And Antibacterial Properties Of Stable Silver Substituted Hydroxyapatite Nanoparticles Synthesized Through Surfactant Assisted Microwave Process., Iqbal N, Abdul Kadir Mr, Nik Malekb Nan, Mahmooda Nhb, Murali Mr, Kamarul T

Murali Malliga Raman

The present study reports a relatively simple method for the synthesis of stable silver substituted hydroxyapatite nanoparticles with controlled morphology and particle size. In order to achieve this, CTAB is included as a surfactant in the microwave refluxing process (600 W for 7 min). The nanoparticles produced with different silver ion concentrations (0.05, 0.1 and 0.2 wt%) were characterized using X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscope (FESEM), energy dispersive X-ray (EDX) and Brunauer–Emmett–Teller (BET) analysis. XRD and FTIR analyses reveal that the Ag-HA nanoparticles were phase pure at ...


Pva-Chitosan Composite Hydrogel Versus Alginate Beads As A Potential Mesenchymal Stem Cell Carrier For The Treatment Of Focal Cartilage Defects: An In Vivo Study Using Rabbits, Dashtdar H, Murali Mr, Abbas Aa, Suhaeb Am, Selvaratnam L, Tay Lx, Kamarul T Jan 2013

Pva-Chitosan Composite Hydrogel Versus Alginate Beads As A Potential Mesenchymal Stem Cell Carrier For The Treatment Of Focal Cartilage Defects: An In Vivo Study Using Rabbits, Dashtdar H, Murali Mr, Abbas Aa, Suhaeb Am, Selvaratnam L, Tay Lx, Kamarul T

Murali Malliga Raman

PURPOSE: To investigate whether mesenchymal stem cells (MSCs) seeded in novel polyvinyl alcohol (PVA)-chitosan composite hydrogel can provide comparable or even further improve cartilage repair outcomes as compared to previously established alginate-transplanted models. METHODS: Medial femoral condyle defect was created in both knees of twenty-four mature New Zealand white rabbits, and the animals were divided into four groups containing six animals each. After 3 weeks, the right knees were transplanted with PVA-chitosan-MSC, PVA-chitosan scaffold alone, alginate-MSC construct or alginate alone. The left knee was kept as untreated control. Animals were killed at the end of 6 months after transplantation ...


The Use Of X-Shaped Cross-Link In Posterior Spinal Constructs Improves Stability In Thoracolumbar Burst Fracture: A Finite Element Analysis, Alizadeh M, Kadir Mr, Fadhli Mm, Fallahiarezoodar A, Azmi B, Murali Malliga Raman, Kamarul T Jan 2013

The Use Of X-Shaped Cross-Link In Posterior Spinal Constructs Improves Stability In Thoracolumbar Burst Fracture: A Finite Element Analysis, Alizadeh M, Kadir Mr, Fadhli Mm, Fallahiarezoodar A, Azmi B, Murali Malliga Raman, Kamarul T

Murali Malliga Raman

Posterior instrumentation is a common fixation method used to treat thoracolumbar burst fractures. However, the role of different cross-link configurations in improving fixation stability in these fractures has not been established. A 3D finite element model of T11-L3 was used to investigate the biomechanical behavior of short (2 level) and long (4 level) segmental spine pedicle screw fixation with various cross-links to treat a hypothetical L1 vertebra burst fracture. Three types of cross-link configurations with an applied moment of 7.5 Nm and 200 N axial force were evaluated. The long construct was stiffer than the short construct irrespective of ...


In Silico, In Vitro And In Vivo Assessment Of Safety And Anti-Inflammatory Activity Of Curcumin., Venkata M, Sripathy R, Anjana D, Somashekara N, Krishnaraju A, Krishanu S, Murali Mr, Verma Sr, Ramchand Cn Jan 2012

In Silico, In Vitro And In Vivo Assessment Of Safety And Anti-Inflammatory Activity Of Curcumin., Venkata M, Sripathy R, Anjana D, Somashekara N, Krishnaraju A, Krishanu S, Murali Mr, Verma Sr, Ramchand Cn

Murali Malliga Raman

Problem statement: Curcumin the active component of turmeric is known for its wide biological actions. Extensive studies on curcumin highlighted its anti-inflammatory, anti-oxidant, anti-microbial, anti-carcinogenic and anti-coagulant activity. The anti-inflammatory activities of curcumin have been demonstrated both in vitro and in vivo. Though curcumin and its anti-inflammatory properties are well documented, the exact mechanism of action and effective in vivo dosage required for potential anti-inflammatory activity of curcumin are yet to be determined. The current work reflects in identfying the the role curcumin in the inflmmatory cascade and arriving at an optimal effective dose (ED50). Approach: The objective of the ...


Differential Expression Of Gastric Muc5ac In Colonic Epithelial Cells: Tff-3 Wired Il1Β/Akt Crosstalk Induced Mucosal Immune Response Against Shigella Dysenteriae Infection, Raja Sb, Murali Mr, Devaraj H, Devaraj Sn Jan 2012

Differential Expression Of Gastric Muc5ac In Colonic Epithelial Cells: Tff-3 Wired Il1Β/Akt Crosstalk Induced Mucosal Immune Response Against Shigella Dysenteriae Infection, Raja Sb, Murali Mr, Devaraj H, Devaraj Sn

Murali Malliga Raman

An understanding of the signaling mechanism(s) that regulate the differential expression of gastric mucin MUC5AC in colonic epithelial cells would contribute significantly to investigations of its role in colonic mucosa infected with the bacterial pathogen Shigella dysenteriae. Here we show that S. dysenteriae-Sinduced expression of interleukin-1β upregulates MUC2 expression and the differential expression of MUC5AC. Differential expression of MUC5AC involves crosstalk between interleukin-1β and Akt, whereby the trefoil factor family peptide TFF3 activates Akt by phosphorylation of EGFR. TFF3 also downregulates E-cadherin expression, causing accumulation of β-catenin in the cytosol. Phosphorylation of GSK-3β (inactivated) by activated Akt inhibits ubiquitylation ...


Mechanical And Functional Assessment Of The Wrist Affected By Rheumatoid Arthritis: A Finite Element Analysis, Bajuri Mn, Kadir Mr, Murali Mr, Kamarul T Jan 2012

Mechanical And Functional Assessment Of The Wrist Affected By Rheumatoid Arthritis: A Finite Element Analysis, Bajuri Mn, Kadir Mr, Murali Mr, Kamarul T

Murali Malliga Raman

Understanding the pathomechanics involved in rheumatoid arthritis (RA) of the wrist provides valuable information, which will invariably allow various therapeutic possibilities to be explored. The computational modelling of this disease permits the appropriate simulation to be conducted seamlessly. A study that underpins the fundamental concept that produces the biomechanical changes in a rheumatoid wrist was thus conducted through the use of finite element method. The RA model was constructed from computed tomography datasets, taking into account three major characteristics: synovial proliferation, cartilage destruction and ligamentous laxity. As control, a healthy wrist joint model was developed in parallel and compared. Cartilage ...


Isolation And Partial Characterization Of A Novel Lectin From Aegle Marmelos Fruit And Its Effect On Adherence And Invasion Of Shigella To Ht29 Cells, Raja Sb, Murali Mr, Kumar Nk, Devaraj Sn Jan 2011

Isolation And Partial Characterization Of A Novel Lectin From Aegle Marmelos Fruit And Its Effect On Adherence And Invasion Of Shigella To Ht29 Cells, Raja Sb, Murali Mr, Kumar Nk, Devaraj Sn

Murali Malliga Raman

Lectins are a class of ubiquitous proteins/glycoproteins that are abundantly found in nature. Lectins have unique carbohydrate binding property and hence have been exploited as drugs against various infectious diseases. We have isolated one such novel lectin from the fruit pulp of Aegle marmelos. The isolated lectin was partially characterised and its effect against Shigella dysenteriae infection was evaluated. The isolated lectin was found to be a dimeric protein with N-acetylgalactosamine, mannose and sialic acid binding specificity. The effect of Aegle marmelos fruit lectin on the adherence of Shigella dysenteriae to human colonic epithelial cells (HT29 cells) was evaluated ...


Determining The Effectiveness Of The Different Preparation Protocols For Platelet-Rich Plasma (Prp) In Yielding Higher Concentrations Of Platelets, Kavitha G, Sangeetha Vn, Shani S, Murali Mr, Raja Ea, Rukmanikanthan S, David C Jan 2011

Determining The Effectiveness Of The Different Preparation Protocols For Platelet-Rich Plasma (Prp) In Yielding Higher Concentrations Of Platelets, Kavitha G, Sangeetha Vn, Shani S, Murali Mr, Raja Ea, Rukmanikanthan S, David C

Murali Malliga Raman

INTRODUCTION: Despite the various methods described in producing platelet-rich plasma (PRP), it is well established that this biological product in its many preparations have been proven to enhance wound healing. However, very little have been known about the efficacy of these methods hence there is a lack of evidence in the superiority of one method over another. Thus, a study was conducted to compare these different protocols to determine which produces the highest concentration of platelets. METHODS: Peripheral blood was obtained from 24 healthy volunteers. Four different protocols using similar 2 step centrifugation methods of preparing PRP were applied to ...


Imperatorin A Furocoumarin Inhibits Periplasmic Cu-Zn Sod Of Shigella Dysenteriae Their By Modulates Its Resistance Towards Phagocytosis During Host Pathogen Interaction, Raja Sb, Murali Mr, Roopa K, Devaraj Sn Jan 2011

Imperatorin A Furocoumarin Inhibits Periplasmic Cu-Zn Sod Of Shigella Dysenteriae Their By Modulates Its Resistance Towards Phagocytosis During Host Pathogen Interaction, Raja Sb, Murali Mr, Roopa K, Devaraj Sn

Murali Malliga Raman

Shigella dysenteriae continues to be a major health problem, which leads to death, due to diarrhoea and dysentery, predominantly in children below the age of 5. Bacterial invasion of the colonic epithelium leads to severe inflammation together with bacterial dissemination generates abscesses and ulcerations. Periplasmic copper, zinc super oxide dismutase of Shigella protects it from exogenous superoxide produced by host, during its invasion. Hence, in present study an attempt was made to study the effect of aqueous extract of Aegle marmelos on host and pathogen defence. Histology analysis of rat ileal loop showed the loss of virulence in aqueous extract ...


Neutralization Of Radical Toxicity By Temperature-Dependent Modulation Of Extracellular Sod Activity In Coral Bleaching Pathogen Vibrio Shiloi And Its Role As A Virulence Factor, Murali Mr, Raja Sb, Devaraj Sn Aug 2010

Neutralization Of Radical Toxicity By Temperature-Dependent Modulation Of Extracellular Sod Activity In Coral Bleaching Pathogen Vibrio Shiloi And Its Role As A Virulence Factor, Murali Mr, Raja Sb, Devaraj Sn

Murali Malliga Raman

Vibrio shiloi is the first and well-documented bacterium which causes coral bleaching, particularly, during summer, when seawater temperature is between 26 and 31 degrees C. Coral bleaching is the disruption of the symbiotic association between coral hosts and their photosynthetic microalgae zooxanthellae. This is either due to lowered resistance in corals to infection or increased virulence of the bacterium at the higher sea surface temperature. The concentration of the oxygen and resulting oxygen radicals produced by the zooxanthellae during photosynthesis are highly toxic to bacteria, which also assist corals in resisting the infection. Hence, in this study we examined the ...


Lactobacilli Inhibit Shigella Dysenteriae 1 Induced Pro-Inflammatory Response And Cytotoxicity In Host Cells Via Impediment Of Shigella-Host Interactions, Murali Mr, Moorthy G, Niranjali Devaraj S Jan 2010

Lactobacilli Inhibit Shigella Dysenteriae 1 Induced Pro-Inflammatory Response And Cytotoxicity In Host Cells Via Impediment Of Shigella-Host Interactions, Murali Mr, Moorthy G, Niranjali Devaraj S

Murali Malliga Raman

OBJECTIVE: Shigella dysenteriae Type 1 dysentery is a major cause of morbidity and mortality in children from less developed and developing countries. The present study explores the hypothesis that lactobacilli protect the host cell during S. dysenteriae Type 1 infection and its mechanism of action. METHODS: Caco-2 cells incubated for 1h with Lactobacillus rhamnosus or Lactobacillus acidophilus at the multiplicity of infection of 100, either alone or in combination followed by addition of Shigella at the same multiplicity of infection for 5h served as treatment groups. Cells incubated with Shigella without lactobacilli addition served as infected cells. At the end ...


Effect Of Aqueous Extract Of Aegle Marmelos Fruit On Adherence And Β-Lactam Resistance Of Enteropathogenic Escherichia Coli By Down Regulating Outer Membrane Protein C, Murali Malliga Raman Jan 2009

Effect Of Aqueous Extract Of Aegle Marmelos Fruit On Adherence And Β-Lactam Resistance Of Enteropathogenic Escherichia Coli By Down Regulating Outer Membrane Protein C, Murali Malliga Raman

Murali Malliga Raman

Problem statement: Enteropathogenic Escherichia Coli (EPEC) continue to be a major health problem, leading to death due to diarrhea, predominantly in children below the age of five. Due to evolution of multi drug resistance in EPEC and side effects caused to host by antibiotics necessitated a search for alternative medicines from medicinal plants. One such medicinal plant used since ancient times to cure diarrhea is Aegle marmelos. This study was done to investigate the effect of aqueous extract of Aegle marmelos fruit (AEAM) on outer membrane protein C (OmpC) of EPEC, which plays a key role in adherence and antibiotic ...


Differential Expression Of Ompc And Ompf In Multidrug-Resistant Shigella Dysenteriae And Shigella Flexneri By Aqueous Extract Of Aegle Marmelos, Altering Its Susceptibility Toward Beta-Lactam Antibiotics, Raja Sb, Murali Mr, Devaraj Sn Jan 2008

Differential Expression Of Ompc And Ompf In Multidrug-Resistant Shigella Dysenteriae And Shigella Flexneri By Aqueous Extract Of Aegle Marmelos, Altering Its Susceptibility Toward Beta-Lactam Antibiotics, Raja Sb, Murali Mr, Devaraj Sn

Murali Malliga Raman

Steadily increasing resistance among Shigella to beta-lactams, aminoglycosides, and tetracycline has compromised the utility of these commonly used antimicrobial agents. Also, undesirable side effects of certain antibiotics have triggered immense interest in search of alternative therapies using medicinal plants. One such medicinal plant used since ancient times to cure diarrhea is Aegle marmelos. The present study exemplifies the susceptibility of beta-lactam-resistant Shigella dysenteriae and Shigella flexneri toward beta-lactam antibiotics, when grown in the presence of aqueous extract of A. marmelos (AEAM), by altering porin channels. This was demonstrated by antibiotic sensitivity test using disc diffusion method and MIC test. Susceptibility ...


Protective Role Of Lactobacilli In Shigella Dysenteriae 1-Induced Diarrhea In Rats., Moorthy G, Murali Malliga Raman, Devaraj Sn Jan 2007

Protective Role Of Lactobacilli In Shigella Dysenteriae 1-Induced Diarrhea In Rats., Moorthy G, Murali Malliga Raman, Devaraj Sn

Murali Malliga Raman

OBJECTIVE: Studies on lactic acid bacteria exemplify their use against various enteropathogens in vitro. Nevertheless, in vivo effects of Lactobacillus during Shigella infection have not been evaluated. The present study evaluated the effect of Lactobacillus rhamnosus and Lactobacillus acidophilus on neutrophil infiltration and lipid peroxidation during Shigella dysenteriae 1-induced diarrhea in rats. METHODS: The rats were divided into eight groups (n = 6 in each group). Induced rats received single oral dose of S. dysenteriae (12 x 10(8) colony-forming units [cfu]/mL). Treated rats received L. rhamnosus (1 x 10(7) cfu/mL) or L. acidophilus (1 x 10(7 ...