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Full-Text Articles in Life Sciences

An Orange Fluorescent Protein Tagging System For Real-Time Pollen Tracking, J. Rice, R. Mundell, R. Millwood, O. Chambers, L. Abercrombie, H. Davies, C. Stewart Dec 2102

An Orange Fluorescent Protein Tagging System For Real-Time Pollen Tracking, J. Rice, R. Mundell, R. Millwood, O. Chambers, L. Abercrombie, H. Davies, C. Stewart

C. Neal Stewart Jr.

Background Monitoring gene flow could be important for future transgenic crops, such as those producing plant-made-pharmaceuticals (PMPs) in open field production. A Nicotiana hybrid (Nicotiana. tabacum × Nicotiana glauca) shows limited male fertility and could be used as a bioconfined PMP platform. Effective assessment of gene flow from these plants is augmented with methods that utilize fluorescent proteins for transgenic pollen identification. Results We report the generation of a pollen tagging system utilizing an orange fluorescent protein to monitor pollen flow and as a visual assessment of transgene zygosity of the parent plant. This system was created to generate a tagged ...


Titus Thesis Figures.Docx, Titus Hou May 2020

Titus Thesis Figures.Docx, Titus Hou

Titus Hou

No abstract provided.


Titus Thesis.Docx, Titus Hou May 2020

Titus Thesis.Docx, Titus Hou

Titus Hou

No abstract provided.


Máy Lọc Nước Điện Giải Kangen Leveluk K8 Nhật Bản Chính Hãng, Nhung Seven Dec 2019

Máy Lọc Nước Điện Giải Kangen Leveluk K8 Nhật Bản Chính Hãng, Nhung Seven

Nhung Seven

Giới thiệu sản phẩm máy lọc nước điện giải kangen leveluk k8 Nhật Bản chính hãng đến từ thương hiệu kangen water (Enagic). Máy điện giải leveluk k8 (Kangen 8) là loại máy chống oxy hóa mạnh mẽ nhất của Enagic – có 8 tấm titan platin nhúng để cải thiện ion hóa nước và tăng khả năng sản xuất chất chống oxy hoá.

Máy kangen k8 là sản phẩm được nhiều người quan tâm nhất trong thời gian hiện tại với khả năng điện phân cao nhất mang lại nguồn nước sạch dinh dưỡng đảm bảo sức khỏe người dùng ...


Jual Obat Aborsi Malang 082242266717 Toko Obat Aborsi Di Malang, Acep Shop Nov 2019

Jual Obat Aborsi Malang 082242266717 Toko Obat Aborsi Di Malang, Acep Shop

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Testimoni Manfaat Minyak Bulus Untuk Wajah Memutihkan Dengan Harga Yang Murah, Ripan Maki Oct 2019

Testimoni Manfaat Minyak Bulus Untuk Wajah Memutihkan Dengan Harga Yang Murah, Ripan Maki

ripan maki

Testimoni manfaat minyak bulus untuk wajah memutihkan dengan harga yang murah.

Minyak bulus adalah ramuan atau obat tradisional ini terbuat dari hewan bulus khusus di kalimantan yang sudah di olah sedemikian rupa hingga menghasilkan berbentuk minyak. Kandungan yang ada berupa vitamin E yang sangat banyak khasiat manfaatnya bisa untuk perawatan kulit.

Pembuatan minyak bulus sendiri ada bermacam-macam cara namun yang terbaik adalah di oleh dengan cara di keringkan atau di jemur dibawah cahaya matahari. Lemak lemak akan meleleh karena panas hingga cair.
Karena sangat terkenal nya minyak bulus sering di cari untuk merawat kesehatan kulit. Harganya yang terjangkau dan aman ...


Ưu Điểm Của Lắp Điện Mặt Trời Hòa Lưới Áp Mái Tại Võ Gia Solar, Võ Gia Solar Jul 2019

Ưu Điểm Của Lắp Điện Mặt Trời Hòa Lưới Áp Mái Tại Võ Gia Solar, Võ Gia Solar

Võ Gia Solar


Where To Buy Provillus Review: Cost, Pills, Ingredient & Side Effect!, Provillus Villus Jul 2019

Where To Buy Provillus Review: Cost, Pills, Ingredient & Side Effect!, Provillus Villus

Provillus villus

The beneficial thing is the way that, lifting weights pro Vincent has assembled unmistakable methodology, approach and simple guidance exercises directly into a framework called Nononsense Musclebuilding that has been demonstrated to help a huge number of lucky people construct muscle rapidly.
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Keto Fast Diet Reviews Pills, Cost & Ingredient, Keto Fast Diet Diet Jul 2019

Keto Fast Diet Reviews Pills, Cost & Ingredient, Keto Fast Diet Diet

Keto Fast Diet Diet

As an adolescent, Isabel saw both her folks and grandparents battling with sort 2 diabetes, and ended up resolved to ponder what is important to counteract sickness. So breathe easy because of the way that you generally have a decision, and you can settle on the decision to relinquish win or bust reasoning. The puppy may likewise have inhalant hypersensitivities from form, residue, or dust. Yet, for best outcomes, devotees are required to entirely pursue the eating routine.
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"Buy" Naturalean Ketones: Review By Shark Tank Diet Pills Ingredients & Side Effects!, Naturalean Ketones Jul 2019

"Buy" Naturalean Ketones: Review By Shark Tank Diet Pills Ingredients & Side Effects!, Naturalean Ketones

Naturalean Ketones

Utilization of is clear as it has cases which can be overwhelmed by standard water by and large it is referenced that confirmation of two cases are sufficient for multi day, one in the underlying fragment of the day preceding the morning supper and other is in the night going before the supper. For point by point rules, you can look for after the techniques referenced outwardly indication of .
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Real-Time Stealth Intervention For Motor Learning Using Player Flow-State, Ramin Tadayon, Ashish Amresh, Troy Mcdaniel, Sethuraman Panchanathan Jun 2019

Real-Time Stealth Intervention For Motor Learning Using Player Flow-State, Ramin Tadayon, Ashish Amresh, Troy Mcdaniel, Sethuraman Panchanathan

Ashish Amresh

We present a novel approach to real-time adaptation in serious games for at-home motor learning. Our approach assesses and responds to the “flow-state” of players by tracking and classifying facial emotions in real-time using the Kinect camera. Three different approaches for stealth assessment and adaptation using performance and flow-state data are defined, along with a case-study evaluation of these approaches based on their effectiveness at maintaining positive affective interaction in a subject.


The Farm Management Extension Audience Of 2030, Alejandro Plastina, Kelvin Leibold, Matthew Stockton Jun 2019

The Farm Management Extension Audience Of 2030, Alejandro Plastina, Kelvin Leibold, Matthew Stockton

Alejandro Plastina

During the halftime of his son’s football game, Farmer Brown asks his smartphone for a real-time cost-effectiveness analysis of three alternative treatments to suppress weeds in two acres over the Missouri River, where remote imagery technology identified weed pressure trending up and nearing 90% of the acre- and corn-stage-specific weed threshold. The device calculates real-time partial budget analyses using acre-specific weather forecasts, soil conditions, labor and machinery costs, corn growth stage and projected yields, weed species, and cutting-edge technology to respond with information about the cost-effectiveness of reducing weed pressure to 75%, 80%, and 85% of the threshold as ...


Cara Menghilangkan Pembengkakan Ambeien, Mengatasi Wasirdewa Jun 2019

Cara Menghilangkan Pembengkakan Ambeien, Mengatasi Wasirdewa

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Alamat Toko - Jual Obat Herbal Bentrap Di Jakarta Selatan 082223546868, Jual Obat Bius Jun 2019

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Alamat Toko - Jual Obat Herbal Bentrap Di Depok 082223546868, Jual Obat Bius Jun 2019

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Alamat Toko - Jual Obat Herbal Bentrap Di Makassar 082223546868, Jual Obat Bius Jun 2019

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Do Cover Crops Pay? Net Returns To Corn/Soybean Farmers In Iowa, Alejandro Plastina Jun 2019

Do Cover Crops Pay? Net Returns To Corn/Soybean Farmers In Iowa, Alejandro Plastina

Alejandro Plastina

COVER CROPS, which are planted on approximately 700,000 of Iowa’s 30 million acres of farmland, have been found to have varying net returns based on several factors— cover c rop species, planting technique, termination method, tillage practices, following cash crop, and the farmer’s years of experience with cover crops.


Maize System Impacts Of Cover Crop Management Decisions: A Simulation Analysis Of Rye Biomass Response To Planting Populations In Iowa, U.S.A., Guillermo S. Marcillo, Sarah Carlson, Meghan Filbert, Tom C. Kaspar, Alejandro Plastina, Fernando E. Miguez Jun 2019

Maize System Impacts Of Cover Crop Management Decisions: A Simulation Analysis Of Rye Biomass Response To Planting Populations In Iowa, U.S.A., Guillermo S. Marcillo, Sarah Carlson, Meghan Filbert, Tom C. Kaspar, Alejandro Plastina, Fernando E. Miguez

Alejandro Plastina

Cover crops provide environmental services that can effectively reduce the negative impacts from otherwise highly productive row-crop systems in the US Midwest. In this context, winter rye [(Secale cereale sp.)] is the most commonly used cover crop among producers because it overwinters and produces considerable biomass in the spring. While the soil and water benefits of a maize-rye system are well documented, the extent to which these benefits change under different rye planting densities has not been fully explored. In particular, shoot-biomass of a fall-seeded rye cover crop is expected to respond to increasing plant populations (PP), influence maize system ...


Is Arc-Co Acting As A Safety Net Program? Evidence From Iowa, Alejandro Plastina, Chad Hart Jun 2019

Is Arc-Co Acting As A Safety Net Program? Evidence From Iowa, Alejandro Plastina, Chad Hart

Alejandro Plastina

THE AGRICULTURAL Act of 2014, referred to as the 2014 Farm Bill, is the legislative backbone of federal farm income support programs and agricultural disaster assistance programs. These programs, combined with federal crop insurance, are what is typically referred to as the farm safety net. As the debate has begun for the next version of the Farm Bill, policy discussions have centered on improving the effectiveness of the safety net. However, in previous Farm Bills, there had been a concerted effort to utilize decoupled agricultural support to ensure that US farm programs would meet World Trade Organization (WTO) standards. The ...


Event Turnament Turnover Terbesar | Pokergocap, Joni Gocap Jun 2019

Event Turnament Turnover Terbesar | Pokergocap, Joni Gocap

Joni Gocap

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A Critical Analysis Of Powell’S Results On The Interdivision Time Distribution, Vincent Quedeville, Jérôme Morchain, Philippe Villedieu, Rodney O. Fox Jun 2019

A Critical Analysis Of Powell’S Results On The Interdivision Time Distribution, Vincent Quedeville, Jérôme Morchain, Philippe Villedieu, Rodney O. Fox

Rodney O. Fox

The cell-age and interdivision-time probability density functions (PDFs) have been extensively investigated since the 1940s due to their fundamental role in cell growth. The pioneering work of Powell established the first relationship between the interdivision-time and cell-age PDFs. In the literature, two definitions for the interdivision-time PDF have been proposed. One stands for the age-at-rupture PDF and is experimentally observable, whereas the other is the probability density that a cell divides at a certain age and is unobservable. From Powell’s results pertaining to the unobservable interdivision-time PDF, Painter and Marr derived an inequality that is true but is incorrectly ...


Quantification Of Site Layout And Filter Characteristics On Primary Filter Airflow Reduction On Commercial Swine Sites In Iowa, Benjamin Smith, Steven Hoff, Jay Harmon, Daniel Andersen, Jeffrey Zimmerman, John Stinn Jun 2019

Quantification Of Site Layout And Filter Characteristics On Primary Filter Airflow Reduction On Commercial Swine Sites In Iowa, Benjamin Smith, Steven Hoff, Jay Harmon, Daniel Andersen, Jeffrey Zimmerman, John Stinn

Jay D. Harmon

Fresh air intake filtration is used on commercial swine breeding-gestation-farrowing farms to reduce the frequency of airborne infectious agents. For swine producers, porcine reproductive and respiratory syndrome virus (PRRSV), influenza A virus and Mycoplasma hyopneumoniae are considered the most economically challenging airborne pathogens. Reduced frequency of disease outbreaks has been attributed to retrofitting existing systems with filtration. Economic analysis of operating costs includes energy use, maintenance and replacement of filters. Filter replacement, the largest operational cost, is dependent on filter lifespan. However, limited data is available on filter lifespan and the rate of airflow reduction during the high dust-loading periods ...


A Sophomore Level Introduction To Engineering Design Course, Norman Muzzy, Michelle L. Soupir, Steven J. Hoff Jun 2019

A Sophomore Level Introduction To Engineering Design Course, Norman Muzzy, Michelle L. Soupir, Steven J. Hoff

Steven J. Hoff

Iowa State University has a sophomore level course that is known as ABE 218. The objectives of this course include learning a structured approach to engineering design, preparing the students for internships, learning the basics of project management, and connecting the theoretical engineering paper designs to physical reality. This informal paper discusses how the course is structured, identifies the lab projects that are part of the course, and discusses some of the results and challenges associated with ABE 218.


Swine Finishing Room Air Infiltration: Part 2. Infiltration As Affected By Room Characteristics, Harishchandra T. Jadhav, Steven J. Hoff, Jay D. Harmon, Daniel S. Andersen Jun 2019

Swine Finishing Room Air Infiltration: Part 2. Infiltration As Affected By Room Characteristics, Harishchandra T. Jadhav, Steven J. Hoff, Jay D. Harmon, Daniel S. Andersen

Daniel S. Andersen

Data collected on 17 swine finishing rooms from the Midwest region of the United States was used to study the relationship between infiltration rate and selected room characteristics. Effect of individual room characteristics on room infiltration rate were tested by simple linear regression (SLR) while multiple linear regression (MLR) was used to develop models for improved prediction. SLR results revealed that the total (It) and other (Io; non-curtain/fan locations) swine finishing room infiltration rates were inversely related to room width and directly related to room length and ceiling height. As expected, rooms with higher curtain end pocket overlap, curtain ...


Quantification Of Site Layout And Filter Characteristics On Primary Filter Airflow Reduction On Commercial Swine Sites In Iowa, Benjamin Smith, Steven Hoff, Jay Harmon, Daniel Andersen, Jeffrey Zimmerman, John Stinn Jun 2019

Quantification Of Site Layout And Filter Characteristics On Primary Filter Airflow Reduction On Commercial Swine Sites In Iowa, Benjamin Smith, Steven Hoff, Jay Harmon, Daniel Andersen, Jeffrey Zimmerman, John Stinn

Daniel S. Andersen

Fresh air intake filtration is used on commercial swine breeding-gestation-farrowing farms to reduce the frequency of airborne infectious agents. For swine producers, porcine reproductive and respiratory syndrome virus (PRRSV), influenza A virus and Mycoplasma hyopneumoniae are considered the most economically challenging airborne pathogens. Reduced frequency of disease outbreaks has been attributed to retrofitting existing systems with filtration. Economic analysis of operating costs includes energy use, maintenance and replacement of filters. Filter replacement, the largest operational cost, is dependent on filter lifespan. However, limited data is available on filter lifespan and the rate of airflow reduction during the high dust-loading periods ...


Drainage Water Quality Impacts Of Agricultural Management Practices: Effect Of Manure Application Timing And Cover Crops, Brian Dougherty, Carl Pederson, Michelle Soupir, Dan Andersen Jun 2019

Drainage Water Quality Impacts Of Agricultural Management Practices: Effect Of Manure Application Timing And Cover Crops, Brian Dougherty, Carl Pederson, Michelle Soupir, Dan Andersen

Daniel S. Andersen

The primary objective of this study is to evaluate the impact of various cropping and nutrient management systems on drainage water quality and crop yields. Treatment comparisons evaluate the impact of liquid swine manure application timing, nitrification inhibitor with late fall swine manure application, cereal rye cover crop, and gypsum application. These comparisons will be conducted for multiple years and used to develop appropriate manure and nutrient management practices in order to minimize water contamination potential and enhance the use of swine manure as a nutrient resource.


A Fragment Of Apolipoprotein E4 Leads To The Downregulation Of A Cxorf56 Homologue, A Novel Er-Associated Protein, And Activation Of Bv2 Microglial Cells, Tanner B. Pollock, Jacob M. Mack, Noail F. Isho, Raquel J. Brown, Alexandra E. Oxford, Brad E. Morrison, Eric J. Hayden, Troy T. Rohn Jun 2019

A Fragment Of Apolipoprotein E4 Leads To The Downregulation Of A Cxorf56 Homologue, A Novel Er-Associated Protein, And Activation Of Bv2 Microglial Cells, Tanner B. Pollock, Jacob M. Mack, Noail F. Isho, Raquel J. Brown, Alexandra E. Oxford, Brad E. Morrison, Eric J. Hayden, Troy T. Rohn

Troy T. Rohn

Despite the fact that harboring the apolipoprotein E4 (APOE4) allele represents the single greatest risk factor for late-onset Alzheimer’s disease (AD), the exact mechanism by which apoE4 contributes to disease progression remains unknown. Recently, we demonstrated that a 151 amino-terminal fragment of apoE4 (nApoE41-151) localizes within the nucleus of microglia in the human AD brain, suggesting a potential role in gene expression. In the present study, we investigated this possibility utilizing BV2 microglia cells treated exogenously with nApoE41-151. The results indicated that nApoE41-151 leads to morphological activation of microglia cells through, at least in part, the ...


A Fragment Of Apolipoprotein E4 Leads To The Downregulation Of A Cxorf56 Homologue, A Novel Er-Associated Protein, And Activation Of Bv2 Microglial Cells, Tanner B. Pollock, Jacob M. Mack, Noail F. Isho, Raquel J. Brown, Alexandra E. Oxford, Brad E. Morrison, Eric J. Hayden, Troy T. Rohn Jun 2019

A Fragment Of Apolipoprotein E4 Leads To The Downregulation Of A Cxorf56 Homologue, A Novel Er-Associated Protein, And Activation Of Bv2 Microglial Cells, Tanner B. Pollock, Jacob M. Mack, Noail F. Isho, Raquel J. Brown, Alexandra E. Oxford, Brad E. Morrison, Eric J. Hayden, Troy T. Rohn

Eric J. Hayden

Despite the fact that harboring the apolipoprotein E4 (APOE4) allele represents the single greatest risk factor for late-onset Alzheimer’s disease (AD), the exact mechanism by which apoE4 contributes to disease progression remains unknown. Recently, we demonstrated that a 151 amino-terminal fragment of apoE4 (nApoE41-151) localizes within the nucleus of microglia in the human AD brain, suggesting a potential role in gene expression. In the present study, we investigated this possibility utilizing BV2 microglia cells treated exogenously with nApoE41-151. The results indicated that nApoE41-151 leads to morphological activation of microglia cells through, at least in part, the ...


A Fragment Of Apolipoprotein E4 Leads To The Downregulation Of A Cxorf56 Homologue, A Novel Er-Associated Protein, And Activation Of Bv2 Microglial Cells, Tanner B. Pollock, Jacob M. Mack, Noail F. Isho, Raquel J. Brown, Alexandra E. Oxford, Brad E. Morrison, Eric J. Hayden, Troy T. Rohn Jun 2019

A Fragment Of Apolipoprotein E4 Leads To The Downregulation Of A Cxorf56 Homologue, A Novel Er-Associated Protein, And Activation Of Bv2 Microglial Cells, Tanner B. Pollock, Jacob M. Mack, Noail F. Isho, Raquel J. Brown, Alexandra E. Oxford, Brad E. Morrison, Eric J. Hayden, Troy T. Rohn

Brad E. Morrison

Despite the fact that harboring the apolipoprotein E4 (APOE4) allele represents the single greatest risk factor for late-onset Alzheimer’s disease (AD), the exact mechanism by which apoE4 contributes to disease progression remains unknown. Recently, we demonstrated that a 151 amino-terminal fragment of apoE4 (nApoE41-151) localizes within the nucleus of microglia in the human AD brain, suggesting a potential role in gene expression. In the present study, we investigated this possibility utilizing BV2 microglia cells treated exogenously with nApoE41-151. The results indicated that nApoE41-151 leads to morphological activation of microglia cells through, at least in part, the ...


Structure-Based Design Of Hepatitis C Virus Vaccines That Elicit Neutralizing Antibody Responses To A Conserved Epitope, Brian G. Pierce, Elisabeth N. Boucher, Kurt H. Piepenbrink, Ejemel Monir, Chelsea A. Rapp, William D. Thomas Jr., Eric J. Sundberg, Zhiping Weng, Yan Wang Jun 2019

Structure-Based Design Of Hepatitis C Virus Vaccines That Elicit Neutralizing Antibody Responses To A Conserved Epitope, Brian G. Pierce, Elisabeth N. Boucher, Kurt H. Piepenbrink, Ejemel Monir, Chelsea A. Rapp, William D. Thomas Jr., Eric J. Sundberg, Zhiping Weng, Yan Wang

Kurt Piepenbrink

Despite recent advances in therapeutic options, hepatitis C virus (HCV) remains a severe global disease burden, and a vaccine can substantially reduce its incidence. Due to its extremely high sequence variability, HCV can readily escape the immune response; thus, an effective vaccine must target conserved, functionally important epitopes. Using the structure of a broadly neutralizing antibody in complex with a conserved linear epitope from the HCV E2 envelope glycoprotein (residues 412 to 423; epitope I), we performed structure-based design of immunogens to induce antibody responses to this epitope. This resulted in epitope-based immunogens based on a cyclic defensin protein, as ...