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Eastern Illinois University

1992

Chemical hydration

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An Examination And Correction Of Plant Tissue Culture Basal Medium Formulations, Henry R. Owen, A. Raymond Miller Jan 1992

An Examination And Correction Of Plant Tissue Culture Basal Medium Formulations, Henry R. Owen, A. Raymond Miller

Faculty Research & Creative Activity

The inorganic formulations of fourteen common plant tissue culture basal media were examined from the primary literature. Inaccuracies and errors were found for molecular formulae, chemical hydrations, and molar equivalences for iron/EDTA complexation. A comparison with published basal medium formulations from six commercial suppliers uncovered additional inaccuracies, modifications, and errors, thereby emphasizing the need for investigators to examine and describe medium formulations precisely in future publications.