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California Polytechnic State University, San Luis Obispo

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Articles 31 - 60 of 1132

Full-Text Articles in Life Sciences

Weights And Balances: Integrating Models For Prevention And Response To Southern California Offshore Oil Spills, Carmen Watts Clayton, Amoret Bunn Oct 2016

Weights And Balances: Integrating Models For Prevention And Response To Southern California Offshore Oil Spills, Carmen Watts Clayton, Amoret Bunn

STAR (STEM Teacher and Researcher) Presentations

Licensing offshore oil and gas reserves in the United States waters are overseen by the U.S. Department of the Interior’s Bureau of Safety and Enforcement (BSEE). The licensing application includes planning for any worst-case oil spill scenario between BSEE and the applicant based on lessons learned from historic offshore spills such as the Deepwater Horizon (2010), Exxon Valdez (1989), and the Union Oil Platform Blowout (1969). The process for planning to respond to oil spills involves coordination with multiple agencies, trustees, and stakeholders to ensure that oil spill responses consider multiple factors, including ecologically sensitive species, commercial transportation ...


Soil Moisture On Soledad Ridge In Santa Rosa Island, Channel Islands National Park, Carrie Fong, Kathryn Mceachern, Ken Niessen Oct 2016

Soil Moisture On Soledad Ridge In Santa Rosa Island, Channel Islands National Park, Carrie Fong, Kathryn Mceachern, Ken Niessen

STAR (STEM Teacher and Researcher) Presentations

Santa Rosa Island is one of the Channel Islands off the coast of southern California. Before the island was heavily grazed, Santa Rosa Island is thought to have had large stands of island oak trees, Quercus tomentella, that provided a critical source of water for the ecosystem by creating a “cloud forest”. Wind-borne fog collects on the leaves, branches, and twigs of the island oaks and other native shrubs. Once the water condenses it drips, falls, and soaks into the soil. Introducing cattle and especially sheep to the island has damaged the ecosystem and nearly decimated Santa Rosa Island of ...


Testing Predictions Used To Build An Agrivoltaics Installation On A Small-Scale Educational Model, Katie Kinney, Rebecca Minor, Greg Barron-Gafford Oct 2016

Testing Predictions Used To Build An Agrivoltaics Installation On A Small-Scale Educational Model, Katie Kinney, Rebecca Minor, Greg Barron-Gafford

STAR (STEM Teacher and Researcher) Presentations

Models are valuable tools for explaining and testing systems. Small-scale models can be especially useful for educational purposes. For models to be useful, they have to accurately depict the larger system that they are describing. A novel man-made system, known as an agrivoltaic structure, is being constructed at Biosphere 2 near Oracle, Arizona. The word agrivoltaic is a combination of agriculture and photovoltaics, or solar farming. My research involved creating a small-scale version of this system for educational purposes. The model of this system tested two predictions: that plants will grow better in the shade of a panel and that ...


To Fish Or Not To Fish? What Effect Do California’S Marine Protected Areas Have On Up-And-Coming Kellet’S Whelk Fishery?, Jennifer Greene Oct 2016

To Fish Or Not To Fish? What Effect Do California’S Marine Protected Areas Have On Up-And-Coming Kellet’S Whelk Fishery?, Jennifer Greene

STAR (STEM Teacher and Researcher) Presentations

Kellet’s whelk, Kelletia kelletii, were observed at sample sites throughout their range from Baja California, Mexico, to Monterey, CA to determine patterns of population density. Sample sites in each region were either located within California marine protected areas where take of the Kellet’s whelk in prohibited, or in non-protected areas where the whelks can be fished both commercially and recreationally. Kellet’s whelk population density was compared between all MPA and non-MPA sample sites. These mean densities were also found for sites in Santa Barbara and San Diego near active fishing ports and compared to data from the ...


Studying The Effects Of Serpentine Soil On Adapted And Non-Adapted Species Using Arduino Technology, Kiana Saniee, Edward Himelblau, Brian Paavo Oct 2016

Studying The Effects Of Serpentine Soil On Adapted And Non-Adapted Species Using Arduino Technology, Kiana Saniee, Edward Himelblau, Brian Paavo

STAR (STEM Teacher and Researcher) Presentations

Abstract: Serpentine soils are formed from ultramafic rocks and are represent an extreme environment for plants. Serpentine soils are unique in that they carry high concentrations of heavy metals, are nutrient deficient, particularly in calcium, and have poor water retention capabilities. Although these soils constitute harsh conditions for plant growth, there are a number of species that are adapted and even endemic to serpentine soil. Water retention by commercial potting mix was compared with serpentine soil. Also, serpentine adapted and non-adapted species were grown in both soil treatments and physiological data were collected. We used the Arduino electronic platform to ...


Impacts Of Fog Drip On Survivorship And Growth Of Native Herb And Shrub Seedlings On Santa Rosa Island, Julianne Bradbury, Ken Niessen, Kathryn Mceachern Sep 2016

Impacts Of Fog Drip On Survivorship And Growth Of Native Herb And Shrub Seedlings On Santa Rosa Island, Julianne Bradbury, Ken Niessen, Kathryn Mceachern

STAR (STEM Teacher and Researcher) Presentations

Overgrazing on Santa Rosa Island led to loss of topsoil in ridgeline groves of endemic island oaks (Quercus tomentella). Restoration specialists attempting to mitigate the impacts of wind and water erosion must determine efficient methods of reestablishing native vegetation. Planting pillows, burlap sacks filled with planting mix and attached to the bedrock substrate, may nurture seedlings long enough for their roots to penetrate the underlying sandstone. Since the island’s ridgeline habitat is often inaccessible during the rainy season, restoration efforts are largely confined to the dry summer months, during which condensed fog is an important source of moisture for ...


The Evaluation Of The Response Of American Eels To Rapid Decompression, Christina Macmillan, Alison Colotelo, John Stephenson, Briana Rhode, Trevor Macduff Sep 2016

The Evaluation Of The Response Of American Eels To Rapid Decompression, Christina Macmillan, Alison Colotelo, John Stephenson, Briana Rhode, Trevor Macduff

STAR (STEM Teacher and Researcher) Presentations

Hydropower dams are used throughout the world to generate electricity and dams can negatively affect fish, as they can hinder or block migration or be a source of injury and mortality during passage. When moving downstream, fish can pass through the turbines instead of other routes (e.g. spillway and juvenile bypass system)where there is a sudden decrease in pressure that can cause barotraumas, injuries from the changes in barometric pressure, (e.g., hemorrhaging, embolism, ruptured organs) due to internal gases expanding. To determine the response of American eels, Anguilla rostrate, to rapid decompression, testing was conducted in the ...


Abundance Of Crabs And Predation On Hemigrapsis Oregonensis In Tiscornia Marsh, San Francisco Bay, Alynn Hoffman, Julie Anne Gonzalez, Michelle Marraffini, Andrew L. Chang Sep 2016

Abundance Of Crabs And Predation On Hemigrapsis Oregonensis In Tiscornia Marsh, San Francisco Bay, Alynn Hoffman, Julie Anne Gonzalez, Michelle Marraffini, Andrew L. Chang

STAR (STEM Teacher and Researcher) Presentations

With a carapace width ranging up to 35 mm for adult males and 29 mm for adult females, Hemigrapsis oregonensis is a native shore crab typically found in the rocky intertidal zone along the Northern Pacific coast. Although this habitat provides protection against desiccation as well as changes in temperature, it may also expose H. oregonensis to predators who prefer the same habitat. The goal of this research was to investigate both the predation on H. oregonensis and the abundance of various crabs of Tiscornia Marsh in San Francisco Bay. We hypothesize that the largest predation will occur in the ...


Identification Of Microorganisms Isolated From Mars-Bound Spacecraft Using Maldi-Tof, Melanie K. Phillips Sep 2016

Identification Of Microorganisms Isolated From Mars-Bound Spacecraft Using Maldi-Tof, Melanie K. Phillips

STAR (STEM Teacher and Researcher) Presentations

The primary goal of the Biotechnology and Planetary Protection Group (BPPG) at the Jet Propulsion Laboratory (JPL) is to prevent microbial contamination between Earth and other planetary bodies. This helps preserve the ability to study other worlds as they exist in their natural states and protects our biosphere from extraterrestrial life. The process for identifying microbes located on space-bound hardware in JPL can be more efficient and precise by using the technique called matrix-assisted laser desorption and ionization time of flight (MALDI-TOF). Once this identification technique is fully implemented, the process time will be reduced from two weeks to one ...


Gc Verification Of The Spacecraft Atmosphere Monitor, Jessica S. Castro, Richard D. Kidd, Jeffrey D. Hein Sep 2016

Gc Verification Of The Spacecraft Atmosphere Monitor, Jessica S. Castro, Richard D. Kidd, Jeffrey D. Hein

STAR (STEM Teacher and Researcher) Presentations

International Space Station crew members face the unique challenge of maintaining air quality due to the volatile organic compounds (VOCs) that have the potential to accumulate at unsafe levels. The Spacecraft Atmosphere Monitor (SAM) is a miniature gas chromatograph/mass spectrometer (GCMS) designed to measure major constituents (such as N2, O2 and CO2) and trace VOCs within the cabin of the spacecraft. The gas chromatograph is responsible for separating the sample into its components in order to be characterized. The oven of the gas chromatograph must reach a temperature of 150°C in order to heat constituents ...


Optimizing Gdna Purification And Qpcr Techniques For Specific Detection And Enumeration Of Probiotic Bacteria, Katelynn C. Lee, Jennifer Vanderkelen, Christopher Kitts Sep 2016

Optimizing Gdna Purification And Qpcr Techniques For Specific Detection And Enumeration Of Probiotic Bacteria, Katelynn C. Lee, Jennifer Vanderkelen, Christopher Kitts

STAR (STEM Teacher and Researcher) Presentations

Probiotics are live microorganisms, like bacteria and yeast, which are thought to provide health benefits when ingested. Large manufacturers produce blends of probiotics for supplementing the diets of agriculturally important animals. Commercial probiotic products are labeled with the number of cells present at the time of manufacture. Our assignment was to develop an assay which can be used to quantify the number of specific lactic acid bacteria present in certain probiotic products, thus verifying the number reported on the product labels. This quantification process involves isolating genomic DNA (gDNA) from the given samples and then running the DNA through a ...


Optimization Of A Genomic Editing System Using Crispr/Cas9-Induced Site-Specific Gene Integration, Jillian L. Mccool Ms., Nick Hum, Gabriela G. Loots Aug 2016

Optimization Of A Genomic Editing System Using Crispr/Cas9-Induced Site-Specific Gene Integration, Jillian L. Mccool Ms., Nick Hum, Gabriela G. Loots

STAR (STEM Teacher and Researcher) Presentations

The CRISPR-Cas system is an adaptive immune system found in bacteria which helps protect against the invasion of other microorganisms. This system induces double stranded breaks at precise genomic loci (1) in which repairs are initiated and insertions of a target are completed in the process. This mechanism can be used in eukaryotic cells in combination with sgRNAs (1) as a tool for genome editing. By using this CRISPR-Cas system, in addition to the “safe harbor locus,” ROSAβ26, the incorporation of a target gene into a site that is not susceptible to gene silencing effects can be achieved through few ...


Investigating The Infection And Persistence Of Sindbis Virus In Host Neurons, Gavin Schroter, Sally Hall, Monica Borucki Aug 2016

Investigating The Infection And Persistence Of Sindbis Virus In Host Neurons, Gavin Schroter, Sally Hall, Monica Borucki

STAR (STEM Teacher and Researcher) Presentations

Sindbis virus, an Alphavirus in the Togaviridae family, is an enveloped, single-stranded positive-sense RNA virus. Found mostly in parts of Africa, Australia, Egypt, Philippines, and Northern Europe – it is known to cause Ockelbo or Pogosta disease [1]. This disease is characterized by the sudden onset of fever, headache, and arthralgia; followed by arthritis, rash, fatigue, and muscle pain. The symptoms are gone within 14 days, though cases have shown joint pain to last from 12 months to 2 and a half years [4]. Common to several other viruses, Sindbis is transmitted from birds (its reservoir) to humans via an arthropod ...


Protein Crystallization Tray Protocol Development, Brooke Connell Aug 2016

Protein Crystallization Tray Protocol Development, Brooke Connell

STAR (STEM Teacher and Researcher) Presentations

In the field of protein crystallography the transfer of the protein crystals from the crystallization plate/tray, where the crystals are initially grown, to the site of analysis is a difficult and lengthy process. A new crystallization tray has been designed and 3D printed to eliminate this intermediate step which is called crystal fishing. The crystallization tray allows crystals to be grown directly on grids that are attached to the pins that are used for analysis of the crystal. The efficacy of protein crystallization using the aforementioned tray was tested in order for a protocol to be developed for potential ...


Effects Of Soil Erosion Barriers On Percent Cover And Sediment Size, Michael Perez Aug 2016

Effects Of Soil Erosion Barriers On Percent Cover And Sediment Size, Michael Perez

STAR (STEM Teacher and Researcher) Presentations

Ranching began on Santa Rosa Island in the 1840’s, introducing nonnative megafauna that put selective grazing pressures on endemic species. Dense groves of island oak (Q. tomentella) are aid in sediment deposition and retention. A current restoration effort, involved installing soil erosion barriers, known as wattles, to prevent sediment from being lost upslope and recruit plant growth whose root systems could further stabilize the slope. This experiment was designed to compare percent cover of vegetation growth in areas with and without soil erosion barriers. This was done using the line intercept method (n=42) on three meter transects, measuring ...


Citizen Science Sensor Development - Smap | Soil Moisture Active Passive, Hagop Hovhannesian Aug 2016

Citizen Science Sensor Development - Smap | Soil Moisture Active Passive, Hagop Hovhannesian

STAR (STEM Teacher and Researcher) Presentations

“Detailed monitoring of soil moisture provides a view of how our whole Earth system works.”

The Soil Moisture Active Passive (SMAP) satellite mission was launched in January 2015; its main purpose is to acquire global measurements of soil moisture. SMAP partnered with the GLOBE program (Global Learning and Observations to Benefit the Environment), which is an international program where students collect environmental variables in a scientifically methodical way. SMAP readings and maps have various uses in various fields, which include monitoring drought, predicting floods, assisting in crop productivity, and linking water, energy and carbon cycles. The goal of this project ...


Comparative Analysis Of In Situ Fibronectin Using Tof-Sims, Spi-Ms, And Dropdesi-Ms In A Microfluidic Reactor, Shannon Fasing, Xiao-Ying Yu, Juan Yao, Jiachao Yu Aug 2016

Comparative Analysis Of In Situ Fibronectin Using Tof-Sims, Spi-Ms, And Dropdesi-Ms In A Microfluidic Reactor, Shannon Fasing, Xiao-Ying Yu, Juan Yao, Jiachao Yu

STAR (STEM Teacher and Researcher) Presentations

Fibronectin is an important biomolecule due to its role in cell differentiation, growth, kinesis, and adhesion. Such biological responses are mediated through membrane recognition and signaling; where fibronectin is found. Studying the outer molecular surface of fibronectin allows deeper insight into the microbiological reactions that occur during these processes. In situ mass spectrometry analysis in aqueous solution accurately represents fibronectin’s chemical components, made possible by a vacuum compatible microfluidic reactor, SALVI (System for Analysis at the Liquid Vacuum Interface). SALVI was paired with the analytical tools: time-of-flight secondary ion mass spectrometer (ToF-SIMS), single photon ionization mass spectrometer (SPI-MS) and ...


Variations In Atmospheric 13c/14c Ratios Within A Radiocarbon Preparatory Lab And Implications For Ams Measurements, Kimberly Arias, Alexandra Hedgpeth, Karis Mcfarlane Aug 2016

Variations In Atmospheric 13c/14c Ratios Within A Radiocarbon Preparatory Lab And Implications For Ams Measurements, Kimberly Arias, Alexandra Hedgpeth, Karis Mcfarlane

STAR (STEM Teacher and Researcher) Presentations

At CAMS, we focus on measuring carbon isotopes (12/13/14C) for a variety of applications. High precision radiocarbon AMS measurements are used for measuring atmospheric radiocarbon. The problem with performing high precision radiocarbon AMS measurements is that there’s almost always background contamination introduced during preparation for the AMS. For graphite preparation, dry ice is used in an isopropyl-dry ice mixture to condense H2O in the CO2 extraction and reduction lines. Dry ice is composed of 12/13C and releases carbon into the atmosphere as it sublimes. The dry ice for preparation is held in containers inside and outside ...


Data Development And Analysis Pathways For Marine Mammals And Turtles: Creating A User Interface, Sarina Fernandez, Warren Asfazadour, Eric Archer, Lisa Komoroske Aug 2016

Data Development And Analysis Pathways For Marine Mammals And Turtles: Creating A User Interface, Sarina Fernandez, Warren Asfazadour, Eric Archer, Lisa Komoroske

STAR (STEM Teacher and Researcher) Presentations

A major obstacle in genetic research is developing streamlined methods for analyzing large amounts of data. The statistical computer programming language R provides users with the ability to develop packages containing specific functions in order to create more accessible data analysis pipelines. However, writing code in R can still be intimidating to those with little to no coding experience. Fortunately, the R package shiny provides a framework for developing web applications based on R functions. Using shiny, we developed a user-friendly web application containing functions of the R package strataG. The strataG package contains several functions for summarizing genetic data ...


Automated Detection Of Deep-Sea Animals, Dallas J. Hollis, Duane Edgington, Danelle Cline Jul 2016

Automated Detection Of Deep-Sea Animals, Dallas J. Hollis, Duane Edgington, Danelle Cline

STAR (STEM Teacher and Researcher) Presentations

The Monterey Bay Aquarium Research Institute routinely deploys remotely operated underwater vehicles equipped with high definition cameras for use in scientific studies. Utilizing a video collection of over 22,000 hours and the Video Annotation and Reference System, we have set out to automate the detection and classification of deep-sea animals. This paper serves to explore the pitfalls of automation and suggest possible solutions to automated detection in diverse ecosystems with varying field conditions. Detection was tested using a saliency-based neuromorphic selective attention algorithm. The animals that were not detected were then used to tune saliency parameters. Once objects are ...


Limestone Flora Of The Simonton Corner Quarry Preserve, Rockport, Maine, Usa, Margaret Stern, Ian D. Medeiros, Luka Negoita, N. Rajakaruna May 2016

Limestone Flora Of The Simonton Corner Quarry Preserve, Rockport, Maine, Usa, Margaret Stern, Ian D. Medeiros, Luka Negoita, N. Rajakaruna

Biological Sciences

Limestone is a distinctive substrate that has significant effects on soils and plants. The present study characterizes the diversity of vascular plants, bryophytes, and lichens at the Simonton Corner Quarry Preserve, an abandoned limestone quarry in Rockport, Maine, USA, which was in operation in the late 1800s. We document vascular plant diversity and associated edaphic features (i.e., soil pH and elemental chemistry) using 30 535 meter plots spread throughout the site. For vascular plants, 114 species in 96 genera and 50 families were observed; few of these species are known to prefer calcareous environments, and 38% are nonnative. Conversely ...


A Comparative Study Of The Flora And Soils Of Great Duck And Little Duck Islands, Maine, Usa, Luka Negoita, Matthew Dickinson, Glen H. Mittlehauser, N. Rajakaruna Apr 2016

A Comparative Study Of The Flora And Soils Of Great Duck And Little Duck Islands, Maine, Usa, Luka Negoita, Matthew Dickinson, Glen H. Mittlehauser, N. Rajakaruna

Biological Sciences

Strong environmental gradients and varied land-use practices have generated a mosaic of habitats harboring distinct plant communities on islands on the coast of Maine. Botanical studies of Maine’s islands, however, are generally limited in number and scope. Baseline studies of Maine’s islands are necessary for assessing vegetation dynamics and changes in habitat conditions in relation to environmental impacts imposed by climate change, rising sea levels, invasive species, pests and pathogens, introduced herbivores, and human disturbance. We conducted a survey of the vascular plants and soils of forest, field, and ocean-side communities of Great Duck and Little Duck Islands ...


Short Communication: Effect Of Storage Temperature On The Solubility Of Milk Protein Concentrate 80 (Mpc80) Treated With Nacl Or Kcl, V. Sikand, P.S. Tong, J. Walker, T. Wang, L.E. Rodriguez-Saona Mar 2016

Short Communication: Effect Of Storage Temperature On The Solubility Of Milk Protein Concentrate 80 (Mpc80) Treated With Nacl Or Kcl, V. Sikand, P.S. Tong, J. Walker, T. Wang, L.E. Rodriguez-Saona

Dairy Science

A previous study in our laboratory showed that addition of 150 mM NaCl or KCl into diafiltration water improved the solubility of freshly made milk protein concentrate 80 (MPC80). In the present study, the objectives were (1) to evaluate the solubility of NaCl- or KCl-treated MPC80 samples kept at varying temperatures and then stored for extensive periods at room temperature (21°C ± 1°C); and (2) to determine if MPC80 samples stored at different temperatures and protein conformation can be grouped or categorized together. Freshly manufactured MPC80 samples were untreated (control), processed with NaCl, or processed with KCl. One ...


Culturally Speaking: Ways To Tame Our Sweet Tooth, Phillip Tong Feb 2016

Culturally Speaking: Ways To Tame Our Sweet Tooth, Phillip Tong

Dairy Science

No abstract provided.


Growth Rate Of Copepods In The San Francisco Bay, Brooke Bemowski, Wim Kimmerer, Toni Ignoffo Jan 2016

Growth Rate Of Copepods In The San Francisco Bay, Brooke Bemowski, Wim Kimmerer, Toni Ignoffo

STAR (STEM Teacher and Researcher) Presentations

The San Francisco Estuary is a complex ecosystem. A key part of the foodweb are zooplankton crustaceans, specifically copepods. Many fish, especially the endangered delta smelt, rely on copepods as a food source. Measuring the growth of copepods assists in predictions if enough food is available for delta smelt. Traditionally growth rate is measured through lengthy and involved processes. The objective of this research project is to develop an imaging process to analyze biomass of copepods more efficiently. For this research, field samples of copepods were collected and grown over three days. Subsamples were taken and preserved at three predetermined ...


Resource Partitioning Along Multiple Niche Axes Drives Functional Diversity In Parrotfishes On Caribbean Coral Reefs, Thomas C. Adam, Megan Kelley, Benjamin I. Ruttenberg, Deron E. Burkepile Dec 2015

Resource Partitioning Along Multiple Niche Axes Drives Functional Diversity In Parrotfishes On Caribbean Coral Reefs, Thomas C. Adam, Megan Kelley, Benjamin I. Ruttenberg, Deron E. Burkepile

Biological Sciences

The recent loss of key consumers to exploitation and habitat degradation has significantly altered community dynamics and ecosystem function across many ecosystems worldwide. Predicting the impacts of consumer losses requires knowing the level of functional diversity that exists within a consumer assemblage. In this study, we document functional diversity among nine species of parrotfishes on Caribbean coral reefs. Parrotfishes are key herbivores that facilitate the maintenance and recovery of coral-dominated reefs by controlling algae and provisioning space for the recruitment of corals. We observed large functional differences among two genera of parrotfishes that were driven by differences in diet. Fishes ...


Culturally Speaking: Of Rotary Phones And Dairy Foods, Phillip Tong Dec 2015

Culturally Speaking: Of Rotary Phones And Dairy Foods, Phillip Tong

Dairy Science

No abstract provided.


Partial Calcium Depletion During Membrane Filtration Affects Gelation Of Reconstituted Milk Protein Concentrates, H. Eshpari, R. Jiménez-Flores, P. S. Tong, M. Corredig Dec 2015

Partial Calcium Depletion During Membrane Filtration Affects Gelation Of Reconstituted Milk Protein Concentrates, H. Eshpari, R. Jiménez-Flores, P. S. Tong, M. Corredig

Dairy Science

Milk protein concentrate powders (MPC) with improved rehydration properties are often manufactured using processing steps, such as acidification and high-pressure processing, and with addition of other ingredients, such as sodium chloride, during their production. These steps are known to increase the amount of serum caseins or modify the mineral equilibrium, hence improving solubility of the retentates. The processing functionality of the micelles may be affected. The aim of this study was to investigate the effects of partial acidification by adding glucono-δ-lactone (GDL) to skim milk during membrane filtration on the structural changes of the casein micelles by observing their chymosin-induced ...


Complete Mitochondrial Genome Of The Speckled Dace Rhinichthys Osculus, A Widely Distributed Cyprinid Minnow Of Western North America, Samantha J. Bock, Morgan M. Malley, Sean Lema Oct 2015

Complete Mitochondrial Genome Of The Speckled Dace Rhinichthys Osculus, A Widely Distributed Cyprinid Minnow Of Western North America, Samantha J. Bock, Morgan M. Malley, Sean Lema

Biological Sciences

The speckled dace Rhinichthys osculus (order Cypriniformes), also known as the carpita pinta, is a small cyprinid minnow native to western North America. Here, we report the sequencing of the full mitochondrial genome (mitogenome) of R. osculus from a male fish collected from the Amargosa River Canyon in eastern California, USA. The assembled mitogenome is 16 658 base pair (bp) nucleotides, and encodes 13 protein-coding genes, and includes both a 12S and a 16S rRNA, 22 tRNAs, and a 985 bp D-loop control region. Mitogenome synteny reflects that of other Ostariophysian fishes with the majority of genes and RNAs encoded ...


Two-Ply Channels For Faster Wicking In Paper-Based Microfluidic Devices, Conor K. Camplisson, Kevin M. Schilling, William L. Pedrotti, Howard A. Stone, Andres W. Martinez Oct 2015

Two-Ply Channels For Faster Wicking In Paper-Based Microfluidic Devices, Conor K. Camplisson, Kevin M. Schilling, William L. Pedrotti, Howard A. Stone, Andres W. Martinez

Chemistry and Biochemistry

This article describes the development of porous two-ply channels for paper-based microfluidic devices that wick fluids significantly faster than conventional, porous, single-ply channels. The two-ply channels were made by stacking two single-ply channels on top of each other and were fabricated entirely out of paper, wax and toner using two commercially available printers, a convection oven and a thermal laminator. The wicking in paper-based channels was studied and modeled using a modified Lucas–Washburn equation to account for the effect of evaporation, and a paper-based titration device incorporating two-ply channels was demonstrated.