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Select Listeria Monocytogenes Subtypes Commonly Found In Foods Carry Distinct Nonsense Mutations In Inla, Leading To Expression Of Truncated And Secreted Internalin A, And Are Associated With A Reduced Invasion Phenotype For Human Intestinal Epithelial Cells, K. K. Nightingale, K. Windham, K. E. Martin, M. Yeung, M. Wiedmann Dec 2005

Select Listeria Monocytogenes Subtypes Commonly Found In Foods Carry Distinct Nonsense Mutations In Inla, Leading To Expression Of Truncated And Secreted Internalin A, And Are Associated With A Reduced Invasion Phenotype For Human Intestinal Epithelial Cells, K. K. Nightingale, K. Windham, K. E. Martin, M. Yeung, M. Wiedmann

Biological Sciences

The surface protein internalin A (InlA) contributes to the invasion of human intestinal epithelial cells by Listeria monocytogenes. Screening of L. monocytogenes strains isolated from human clinical cases (n=46), foods (n=118), and healthy animals (n=58) in the United States revealed mutations in inlA leading to premature stop codons (PMSCs) in L. monocytogenes ribotypes DUP-1052A and DUP-16635A (PMSC mutation type 1), DUP-1025A and DUP-1031A (PMSC mutation type 2), and DUP-1046B and DUP-1062A (PMSC mutation type 3). While all DUP-1046B, DUP-1062A, DUP-16635A, and DUP-1031A isolates (n=76) contained inlA PMSCs, ribotypes DUP-1052A and DUP-1025A (n=72) contained isolates with ...


Cd4+ Cell Count, Viral Load, And Highly Active Antiretroviral Therapy Use Are Independent Predictors Of Body Composition Alterations In Hiv-Infected Adults: A Longitudinal Study, Ann Y. Mcdermott, Norma Terrin, Christine Wanke, Sally Skinner, Eric Tchetgen, Abby H. Shevitz Dec 2005

Cd4+ Cell Count, Viral Load, And Highly Active Antiretroviral Therapy Use Are Independent Predictors Of Body Composition Alterations In Hiv-Infected Adults: A Longitudinal Study, Ann Y. Mcdermott, Norma Terrin, Christine Wanke, Sally Skinner, Eric Tchetgen, Abby H. Shevitz

Kinesiology

To understand the concurrent effects of human immunodeficiency virus (HIV) infection, the immune system, and antiretroviral therapy on body composition alterations, we examined annualized composition changes in HIV-infected adults who were receiving stable antiretroviral therapy.


Process For The Preparation Of Citalopram Intermediate, Aslam A. Malik, Hasan Palandoken, Joy A. Stringer, Dershing Huang, Antonio Romero, Olivier Dapremont Nov 2005

Process For The Preparation Of Citalopram Intermediate, Aslam A. Malik, Hasan Palandoken, Joy A. Stringer, Dershing Huang, Antonio Romero, Olivier Dapremont

Chemistry and Biochemistry

The present invention provides, inter alia, a novel process for the preparation of Citalopram, a known antidepressant.


Mypyramid.Gov, Ann Y. Mcdermott Nov 2005

Mypyramid.Gov, Ann Y. Mcdermott

Kinesiology

The US Department of Agriculture (USDA) has un-unveiled a new symbol and an interactive "food guidance system" called "MyPyramid: Steps to a Healthier You," which replaces the 13-year-old Food Guide Pyramid. The new program aims to support the eating and physical activity habits necessary to promote health and prevent such common diseases as obesity, cardiovascular disease, diabetes, osteoporosis, and some cancers. Over the past few decades, population-specific scientific evidence of the effects of nutrition, activity, and body weight on health outcomes has become available. The scientific literature allowed the formulation of new and more detailed recommendations. The USDA's new ...


Performance Of Binary-Encounter-Bethe (Beb) Theory For Electron-Impact Ionization Cross Sections Of Molecules Containing Heavy Elements (Z > 10), Gregory E. Scott, Karl K. Irikura Nov 2005

Performance Of Binary-Encounter-Bethe (Beb) Theory For Electron-Impact Ionization Cross Sections Of Molecules Containing Heavy Elements (Z > 10), Gregory E. Scott, Karl K. Irikura

Chemistry and Biochemistry

The binary-encounter-Bethe (BEB) theory developed by Kim and coworkers has been successful for computing electron-impact ionization cross sections of many molecules. However, some recent publications have stated that BEB theory performs poorly for molecules that contain heavier elements such as chlorine and sulfur. We have found that the BEB calculations in those publications were performed incorrectly. When performed correctly, BEB predictions are as good for heavy-element molecules as for light-element molecules. We recommended recently that an alternative, less-confusing procedure be used for molecules that contain heavier elements. The alternative procedure, based upon effective core potentials (ECPs), does not require explicit ...


Carotenoid Availability Affects The Development Of A Colour-Based Mate Preference And The Sensory Bias To Which It Is Genetically Linked, Gregory F. Grether, Gita R. Kolluru, F. Helen Rodd, Jennifer De La Cerda, Kaori Shimazaki Oct 2005

Carotenoid Availability Affects The Development Of A Colour-Based Mate Preference And The Sensory Bias To Which It Is Genetically Linked, Gregory F. Grether, Gita R. Kolluru, F. Helen Rodd, Jennifer De La Cerda, Kaori Shimazaki

Biological Sciences

Regardless of their origins, mate preferences should, in theory, be shaped by their benefits in a mating context. Here we show that the female preference for carotenoid colouration in guppies (Poecilia reticulata) exhibits a phenotypically plastic response to carotenoid availability, confirming a key prediction of sexual selection theory. Earlier work indicated that this mate preference is genetically linked to, and may be derived from, a sensory bias that occurs in both sexes: attraction to orange objects. The original function of this sensory bias is unknown, but it may help guppies find orange-coloured fruits in the rainforest streams of Trinidad. We ...


Correlates Of Soy Food Consumption In Women At Increased Risk For Breast Cancer, Carolyn Y. Fang, Marilyn Tseng, Mary B. Daly Oct 2005

Correlates Of Soy Food Consumption In Women At Increased Risk For Breast Cancer, Carolyn Y. Fang, Marilyn Tseng, Mary B. Daly

Kinesiology

Objectives The objectives of the present study were to examine sociodemographic correlates of soy food consumption among women at increased risk of breast cancer, describe factors influencing soy food consumption or nonconsumption, and identify women’s sources of information about soy foods. Design A cross-sectional, self-report survey was used to assess frequency of and factors influencing soy food consumption. Soy food intake was reported for the past year. Subjects/setting Participants were 452 women with family histories of breast cancer who were enrolled in a cancer risk assessment program. Statistical analyses performed Comparisons between consumers and nonconsumers of soy foods ...


Obesity In Women, Suzanne Phelan, Rena R. Wing Oct 2005

Obesity In Women, Suzanne Phelan, Rena R. Wing

Kinesiology

No abstract provided.


A Facile Synthesis Of (Tert-Alkoxy)Amines, Hasan Palandoken, Chris M. Bocian, Michelle R. Mccombs, Michael H. Nantz Sep 2005

A Facile Synthesis Of (Tert-Alkoxy)Amines, Hasan Palandoken, Chris M. Bocian, Michelle R. Mccombs, Michael H. Nantz

Chemistry and Biochemistry

Tertiary alcohols react with stoichiometric BF3·Et2O and N-hydroxyphthalimide to yield N-alkoxyphthalimides. Subsequent hydrazinolyses afford the title compounds.


Two-Dimensional Gel Analysis Of The Heat-Shock Response In Marine Snails (Genus Tegula): Interspecific Variation In Protein Expression And Acclimation Ability, Lars Tomanek Aug 2005

Two-Dimensional Gel Analysis Of The Heat-Shock Response In Marine Snails (Genus Tegula): Interspecific Variation In Protein Expression And Acclimation Ability, Lars Tomanek

Biological Sciences

The degree to which temperature acclimation modifies the acute synthesis of the entire heat-shock protein (Hsp) complement is still unknown, but it may constitute an important mechanism for understanding the differences in acclimation ability among closely related ectothermic species that occupy widely varying thermal environments. In general, eurythermal (heat-tolerant) species modify physiological function in response to an increase in acclimation temperature to a greater extent than stenothermal (heat-sensitive) species. In the present work I used 35S-labelled amino acids and two-dimensional gel electrophoresis to test this assumption for how acclimation affects acute Hsp expression (referred to as phenotypic plasticity) in ...


The Clearance Of Protein-Bound Solutes By Hemofiltration And Hemodiafiltration, Timothy W. Meyer, Jason L. Walther, Maria Enrica Pagtalunan, Andres W. Martinez, Ali Torkamani, Patrick D. Fong, Natalie S. Recht, Channing R. Robertson, Thomas H. Hostetter Aug 2005

The Clearance Of Protein-Bound Solutes By Hemofiltration And Hemodiafiltration, Timothy W. Meyer, Jason L. Walther, Maria Enrica Pagtalunan, Andres W. Martinez, Ali Torkamani, Patrick D. Fong, Natalie S. Recht, Channing R. Robertson, Thomas H. Hostetter

Chemistry and Biochemistry

Background. Hemofiltration in the form of continuous venovenous hemofiltration (CVVH) is increasingly used to treat acute renal failure. Compared to hemodialysis, hemofiltration provides high clearances for large solutes but its effect on protein-bound solutes has been largely ignored.

Methods. Standard clinical systems were used to remove test solutes from a reservoir containing artificial plasma. Clearances of the protein-bound solutes phenol red (CPR) and indican (CIN) were compared to clearances of urea (CUREA) during hemofiltration and hemodiafiltration. A mathematical model was developed to predict clearances from values for plasma flow Qp, dialysate flow Qd, ultrafiltration rate ...


Genetic Analysis Of Two Bile Salt Hydrolase Activities In Lactobacillus Acidophilus Ncfm, Olivia Mcauliffe, Raul J. Cano, Todd R. Klaenhammer Aug 2005

Genetic Analysis Of Two Bile Salt Hydrolase Activities In Lactobacillus Acidophilus Ncfm, Olivia Mcauliffe, Raul J. Cano, Todd R. Klaenhammer

Biological Sciences

Two genes, bshA and bshB, encoding bile salt hydrolase enzymes (EC 3.5.1.24) were identified in the genome sequence of Lactobacillus acidophilus NCFM. Targeted inactivation of these genes via chromosomal insertion of an integration vector demonstrated different substrate specificities for these two enzymes.


Elevated Levels Of Trace Elements In Cores Of Otoliths And Their Potential For Use As Natural Tags, Benjamin I. Ruttenberg, Scott L. Hamilton, Michael J.H. Hickford, Georges L. Paradis, Michael S. Sheehy, Julie D. Standish, Ofer Ben-Tzvi, Robert R. Warner Aug 2005

Elevated Levels Of Trace Elements In Cores Of Otoliths And Their Potential For Use As Natural Tags, Benjamin I. Ruttenberg, Scott L. Hamilton, Michael J.H. Hickford, Georges L. Paradis, Michael S. Sheehy, Julie D. Standish, Ofer Ben-Tzvi, Robert R. Warner

Biological Sciences

Variation in the chemical composition of fish otoliths has been used in recent years to address a range of ecological questions, including levels of stock mixing, variation in habitat use, and rates of larval exchange. While some of these questions have been answered with varying success, the degree to which discrete populations are connected via larval exchange remains unknown. To identify larval sources using natural variation in otolith chemistry, we must distinguish and measure the chemical composition of the otolith core, the portion of the otolith formed at the spawning site. Using laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS ...


Patterns, Causes And Consequences Of Regional Variation In The Ecology And Life History Of A Reef Fish, Benjamin I. Ruttenberg, Alison J. Haupt, Angel I. Chiriboga, Robert R. Warner Jul 2005

Patterns, Causes And Consequences Of Regional Variation In The Ecology And Life History Of A Reef Fish, Benjamin I. Ruttenberg, Alison J. Haupt, Angel I. Chiriboga, Robert R. Warner

Biological Sciences

Many species vary in their ecology across their geographic ranges in response to gradients in environmental conditions. Such variation, which can influence life history traits and subsequent demography of populations, usually occurs over large spatial scales. However, describing and understanding the causes of such variation is difficult precisely because it occurs over such large spatial scales. In this study, we document spatial variation in the ecology of a common reef fish, Stegastes beebei, in the Gala´pagos Islands and test a number of potential causal mechanisms. The pattern resembles that seen in latitudinal variation: individuals are larger, occur in higher ...


Structure And Development Of Pitch Canker Infected Monterey Pine Stands At Ano Nuevo, California, Douglas D. Piirto, Sauli Valkonen Jul 2005

Structure And Development Of Pitch Canker Infected Monterey Pine Stands At Ano Nuevo, California, Douglas D. Piirto, Sauli Valkonen

Natural Resources Management and Environmental Sciences

The sustainability of indigenous Monterey pine (Pinus radiata D. Don)at Ano Nuevo stands in the central coast of California was examined. The foremost management objective in these stands is to establish and maintain stand structures that ensure a sustainable presence of the species in terms of uneven-aged management. The major threats are the proliferation of shade-tolerant tree species and the pitch canker ( Fusarium circinatum) disease. The study was based on data from 17 systematically placed sample plots, measured once, in one stand with a very high degree of variation in stand structure and species composition. The results indicated that ...


Adaptive Sampling Of Phytoplankton Responses To Episodic Physical Forcing In The Nearshore Coastal Ocean, Mark A. Moline Jul 2005

Adaptive Sampling Of Phytoplankton Responses To Episodic Physical Forcing In The Nearshore Coastal Ocean, Mark A. Moline

Biological Sciences

The original proposal focused on the linkages between in-water physical, biological and optical dynamics and to assess the degree to which remote sensing could be applied. These areas were approached during the 2001 sampling season and with follow on studies, as part of the extension of the grant through the PECASE award, there are three specific areas of work that have evolved from these original efforts. The first is a further examination of the coherence between convergence zones, general current patterns and spatial distribution of in-water biological signals (phytoplankton). The second is a remote sensing focus, attempting to define the ...


Proliferation Zones In The Salmon Telencephalon And Evidence For Environmental Influence On Proliferation Rate, Sean C. Lema, Mark J. Hodges, Michael P. Marchetti, Gabrielle A. Nevitt Jul 2005

Proliferation Zones In The Salmon Telencephalon And Evidence For Environmental Influence On Proliferation Rate, Sean C. Lema, Mark J. Hodges, Michael P. Marchetti, Gabrielle A. Nevitt

Biological Sciences

Cell proliferation occurs in the brain of fish throughout life. This mitotic activity contributes new neurons to some brain subdivisions, suggesting potential for plasticity in neural development. Recently we found that the telencephalon in salmonids (salmon, trout) is significantly reduced in fish reared in hatcheries compared to wild fish, and that these differences resulted in part from rearing conditions. Here, we describe localized areas of cell proliferation in the telencephalon of juvenile coho salmon (Oncorhynchus kisutch) and begin to explore whether mitotic activity in these areas is sensitive to environmental conditions. Using the 5-bromo-2'-deoxyuridine (BrdU) cell birthdating technique, we ...


Comparison Of Intakes Of Us Chinese Women Based On Food Frequency And 24-Hour Recall Data, Marilyn Tseng, Teresita Hernandez Jul 2005

Comparison Of Intakes Of Us Chinese Women Based On Food Frequency And 24-Hour Recall Data, Marilyn Tseng, Teresita Hernandez

Kinesiology

The objective of this cross-sectional study was to compare dietary reports from a food frequency questionnaire (FFQ) for US Chinese women with 24-hour recall estimates. The subjects were 56 women recruited through organizations in Philadelphia’s Chinese community. Spearman correlations were used to describe FFQ estimates of food servings per month and nutrient intake per day vs estimates from three 24-hour recalls over 1 month. On average, women reported at least weekly consumption of 28 of 96 FFQ food items. The three most frequently consumed were rice (38 times/month), tea (29 times/ month), and dark green, leafy vegetables (18 ...


The National Weight Control Registry: Is It Useful In Helping Deal With Our Obesity Epidemic?, James O. Hill, Holly Wyatt, Suzanne Phelan, Rena Wing Jul 2005

The National Weight Control Registry: Is It Useful In Helping Deal With Our Obesity Epidemic?, James O. Hill, Holly Wyatt, Suzanne Phelan, Rena Wing

Kinesiology

The National Weight Control Registry (NWCR) consists of over 4800 individuals who have been successful in long-term weight loss maintenance. The purpose of establishing the NWCR was to identify the common characteristics of those who succeed in long-term weight loss maintenance. We found very little similarity in how these individuals lost weight but some common behaviors in how they are keeping their weight off. To maintain their weight loss NWCR participants report eating a relatively low-fat diet, eating breakfast almost every day, weighing themselves regularly, and engaging in high levels (about 1 hour/day) of physical activity. Because this is ...


Effects Of Food Supplementation On The Physiological Ecology Of Female Western Diamond‐Backed Rattlesnakes (Crotalus Atrox), Emily N. Taylor, Michael A. Malawy, Dawn M. Browning, Shea V. Lemar, Dale F. Denardo Jun 2005

Effects Of Food Supplementation On The Physiological Ecology Of Female Western Diamond‐Backed Rattlesnakes (Crotalus Atrox), Emily N. Taylor, Michael A. Malawy, Dawn M. Browning, Shea V. Lemar, Dale F. Denardo

Biological Sciences

Food availability is an important factor in the life histories of organisms because it is often limiting and thus can affect growth, mass change, reproduction, and behaviors such as thermoregulation, locomotion, and mating. Experimental studies in natural settings allow researchers to examine the effects of food on these parameters while animals are free to behave naturally. The wide variation among organisms in energy demands and among environmental food resources suggest that responses to changes in food availability may vary among organisms. Since most supplemental feeding field experiments have been conducted on species with high energy demands, we conducted a supplemental ...


Sexual Size Dimorphism And Growth Plasticity In Snakes: An Experiment On The Western Diamond‐Backed Rattlesnake (Crotalus Atrox), Emily N, Taylor, Dale F. Denardo Jun 2005

Sexual Size Dimorphism And Growth Plasticity In Snakes: An Experiment On The Western Diamond‐Backed Rattlesnake (Crotalus Atrox), Emily N, Taylor, Dale F. Denardo

Biological Sciences

We conducted an experiment to examine the effects of sex and food intake on growth, mass gain, and attainment of sexual maturity in Western Diamond‐backed Rattlesnakes (Crotalus atrox). We also measured testosterone levels to determine whether testosterone might be involved in the male‐biased sexual size dimorphism observed in this species. We collected neonate rattlesnakes and raised them in the laboratory for 2 years on either a high‐intake diet (fed one mouse per week) or a low‐intake diet (fed one mouse every 3 weeks). High‐intake snakes grew and gained mass more rapidly than low‐intake snakes ...


Taxonomic Revision And Stratigraphic Provenance Of Histiophorus Rotundus Woodward 1901 (Teleostei, Perciformes), Kenneth A. Monsch, Harry L. Fierstine, Robert E. Weems Jun 2005

Taxonomic Revision And Stratigraphic Provenance Of Histiophorus Rotundus Woodward 1901 (Teleostei, Perciformes), Kenneth A. Monsch, Harry L. Fierstine, Robert E. Weems

Biological Sciences

Until recently, Histiophorus rotundus Woodward 1901, was known from a single, poorly preserved rostrum from the Tertiary phosphate beds near Charleston, South Carolina, an area from which many fossils have been described. The specimen is relatively featureless externally; its internal anatomy is unknown and the documentation of its geological provenance was poor. In an earlier revision the species was transferred to the fossil billfish genus Xiphiorhynchus Van Beneden, 1871. Here we confirm this designation, supported by new morphological studies of the holotype, recently found specimens of tXiphiorhynchus rotundus (Woodward, 1901), and the stratigraphic record of tXiphiorhynchus. The systematic paleontology we ...


Xiphiorhynchus Cf. X. Eocaenicus (Woodward, 1901), (Scombroidei: Xiphiidae: Xiphiorhynchinae) From The Middle Eocene Of Mississippi, The First Transatlantic Distribution Of A Species Of Xiphiorhynchus, Harry L. Fierstine, James E. Starnes Jun 2005

Xiphiorhynchus Cf. X. Eocaenicus (Woodward, 1901), (Scombroidei: Xiphiidae: Xiphiorhynchinae) From The Middle Eocene Of Mississippi, The First Transatlantic Distribution Of A Species Of Xiphiorhynchus, Harry L. Fierstine, James E. Starnes

Biological Sciences

An anterior (distal) rostrum (MMNS 2957) from the Moodys Branch Formation (late middle Eocene). Tesheva Creek, Yazoo County, Mississippi, U.S.A., is described and identified as Xiphiorhynchus cf. X. eocaenicus. The specimen is compared to various species of Xiphiorhynchus, especially the holotype of X. eocaenicl.ls (BMNH 25744), Braeklesham Group, early middle Eocene, England. This represents the first record of a species of Xiphiorhynchl.ls in deposits on both sides of the North Atlantic Ocean. Xiphiorhynchus eocaenicus is thought to have had similar environmental prtoferences and habits as the extant swordfish, Xiphias gladius. Since the swordfish prefers oceanic water ...


A New Aglyptorhynchus (Perciformes: Scombroidei) From The Lincoln Creek Formation (Late Oligocene, Washington, U.S.A.), Harry L. Fierstine Jun 2005

A New Aglyptorhynchus (Perciformes: Scombroidei) From The Lincoln Creek Formation (Late Oligocene, Washington, U.S.A.), Harry L. Fierstine

Biological Sciences

A partial rostrum with an attachcd lower jaw, a posterior neurocranium, and a proximal hyomandibular from the Lincoln Creek Formation (late Oligocene, Washington) arc described and identified as Aglyptorhynchlls coillmhianlls sp, nov. In addition. ten articulated anterior caudal vertebrae presumably from the Lincoln Creek Formation arc described and identified as Aglyptorhyllchlls sp. This is a second record of an Aglyptorhynchus from a deposit bordering the Pacific Ocean, The specimens are compared with other billfishes (Perciformes: Scombroidei), both extant and extinct. Unusual features include a tripartite celous occipital condyle composed equally of the basioccipital and cxoccipitals, a lower jaw that is ...


A Multi-Platform Bathyphotometer For Fine-Scale, Coastal Bioluminescence Research, Christen M. Herren, Steven H.D. Haddock, Cyril Johnson, Cristina M. Orrico, Mark A. Moline, James F. Case May 2005

A Multi-Platform Bathyphotometer For Fine-Scale, Coastal Bioluminescence Research, Christen M. Herren, Steven H.D. Haddock, Cyril Johnson, Cristina M. Orrico, Mark A. Moline, James F. Case

Biological Sciences

Although bioluminescence (BL) in the open ocean has been extensively studied, coastal BL remains poorly understood due, in large degree, to a lack of BL instrumentation appropriate to measure the fine-scale biological and physical complexity of the coastal regime. As a contribution toward understanding coastal BL, we developed the Multipurpose Bioluminescence Bathyphotometer (MBBP). This compact, self-contained bathyphotometer (BP) was designed to function in a variety of deployment modes, including conventional shipboard profilers, towed platforms, autonomous underwater vehicles (AUVs), and profiling moorings. In all configurations, the instrument preserves signal structure at centimeter to meter scale resolution, the scale at which higher-flow ...


Making Babies By The Flip Of A Coin?, Matthew A. Carlton, William D. Stansfield May 2005

Making Babies By The Flip Of A Coin?, Matthew A. Carlton, William D. Stansfield

Biological Sciences

Many probability and genetics textbooks pose standard questions about eye color, birth defects, sexes of children, and so on. Solutions to these questions, specifically about sexes, generally make two assumptions: first, that a randomly selected embryo is equally likely to be male or female; second, that the sexes of successive children from the same parents are independent. In other words, probabilists (and some geneticists) treat sexes of children like flips of a fair coin: two possible outcomes, each equally likely, with outcomes independent from trial to trial. But are these assumptions realistic? Demographic data suggest that neither a balance of ...


Amount Of Food Group Variety Consumed In The Diet And Long-Term Weight Loss Maintenance, Hollie A. Raynor, Robert W. Jeffery, Suzanne Phelan, James O. Hill, Rena R. Wing May 2005

Amount Of Food Group Variety Consumed In The Diet And Long-Term Weight Loss Maintenance, Hollie A. Raynor, Robert W. Jeffery, Suzanne Phelan, James O. Hill, Rena R. Wing

Kinesiology

Objective: Decreases in variety of foods consumed within high-fat-dense food groups and increases in variety of foods consumed within low-fat-dense food groups are associated with lower energy intake and greater weight loss during obesity treatment and may assist with weight loss maintenance. This study examined food group variety in 2237 weight loss maintainers in the National Weight Control Registry, who had lost 32.2 ± 18.0 kg (70.9 ± 39.5 lbs) and maintained a weight loss of at least 13.6 kg (30 lbs) for 6.1 ± 7.7 years.
Research Methods and Procedures: At entry into the registry ...


Biomolecule Detection Via Target Mediated Nanoparticle Aggregation And Dielectrophoretic Impedance Measurement, Philip J. Costanzo, Enzhu Liang, Timothy E. Patten, Scott D. Collins, Rosemary L. Smith Apr 2005

Biomolecule Detection Via Target Mediated Nanoparticle Aggregation And Dielectrophoretic Impedance Measurement, Philip J. Costanzo, Enzhu Liang, Timothy E. Patten, Scott D. Collins, Rosemary L. Smith

Chemistry and Biochemistry

A new biosensing system is described that is based on the aggregation of nanoparticles by a target biological molecule and dielectrophoretic impedance measurement of these aggregates. The aggregation process was verified within a microchannel via fluorescence microscopy, demonstrating that this process can be used in a real time sensor application. Positive dielectrophoresis is employed to capture the nanoparticle aggregates at the edge of thin film electrodes, where their presence is detected either by optical imaging via fluorescence microscopy or by measuring the change in electrical impedance between adjacent electrodes. The electrical detection mechanism demonstrates the potential for this method as ...


The Metalloprotease Of Listeria Monocytogenes Controls Cell Wall Translocation Of The Broad-Range Phospholipase C, P.S. Marie Yeung, Nicholas Zagorski, Helene Marquis Apr 2005

The Metalloprotease Of Listeria Monocytogenes Controls Cell Wall Translocation Of The Broad-Range Phospholipase C, P.S. Marie Yeung, Nicholas Zagorski, Helene Marquis

Biological Sciences

Listeria monocytogenes is a gram-positive bacterial pathogenthat multiplies in the cytosol of host cells and spreads directlyfrom cell to cell. During cell-to-cell spread, bacteria becometemporarily confined to secondary vacuoles. The broad-rangephospholipase C (PC-PLC) of L. monocytogenes contributes tobacterial escape from secondary vacuoles. PC-PLC requires cleavageof an N-terminal propeptide for activation, and Mpl, a metalloproteaseof Listeria, is involved in the proteolytic activation of PC-PLC.Previously, we showed that cell wall translocation of PC-PLCis inefficient, resulting in accumulation of PC-PLC at the membrane-cellwall interface. In infected cells, rapid cell wall translocationof PC-PLC is triggered by a decrease in pH and correlates withcleavage ...


Hiv And Nutrition: Pregnant And Lactating Women, Peggy Papathakis, Nigel Rollins Apr 2005

Hiv And Nutrition: Pregnant And Lactating Women, Peggy Papathakis, Nigel Rollins

Food Science and Nutrition

No abstract provided.