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Full-Text Articles in Life Sciences

The Case For Simplifying Ingredient Statements, Phillip S. Tong Feb 2011

The Case For Simplifying Ingredient Statements, Phillip S. Tong

Dairy Science

No abstract provided.


Food Processors Look To Dairy Ingredients, Phillip S. Tong Dec 2010

Food Processors Look To Dairy Ingredients, Phillip S. Tong

Dairy Science

No abstract provided.


A Novel Method For Evaluating The Release Of Fermentable Sugars From Cellulosic Biomass, Rafael Jiménez-Flores, Gina Fake, Jennifer Carroll, Elizabeth Hood, John Howard Oct 2010

A Novel Method For Evaluating The Release Of Fermentable Sugars From Cellulosic Biomass, Rafael Jiménez-Flores, Gina Fake, Jennifer Carroll, Elizabeth Hood, John Howard

Dairy Science

Efficient hydrolysis of cellulosic biomass into free sugars for the production of bioethanol would allow utilization of an abundant and renewable natural resource. However, complex microcrystalline cellulose in biomass resists digestion. A preferred method to deconstruct cellulose is using enzyme cocktails, but the presence of multiple enzymes with synergistic outcomes for this process complicates direct biochemical analysis as a reliable indicator of deconstruction. Methods to detect release of free sugars rather than direct measurement of enzymatic activity are available but are time-consuming, and do not reflect the production of metabolic inhibitors of microbial growth which may interfere with the subsequent ...


Delivering Health And Wellness, Phillip S. Tong Sep 2010

Delivering Health And Wellness, Phillip S. Tong

Dairy Science

No abstract provided.


The Impact Of Antioxidant Addition On Flavor Of Cheddar And Mozzarella Whey And Cheddar Whey Protein Concentrate, I. W. Liaw, H. Eshpari, P. S. Tong, M. A. Drake Aug 2010

The Impact Of Antioxidant Addition On Flavor Of Cheddar And Mozzarella Whey And Cheddar Whey Protein Concentrate, I. W. Liaw, H. Eshpari, P. S. Tong, M. A. Drake

Dairy Science

Lipid oxidation products are primary contributors to whey ingredient off-flavors. The objectives of this study were to evaluate the impact of antioxidant addition in prevention of flavor deterioration of fluid whey and spray-dried whey protein. Cheddar and Mozzarella cheeses were manufactured in triplicate. Fresh whey was collected, pasteurized, and defatted by centrifugal separation. Subsequently, 0.05% (w/w) ascorbic acid or 0.5% (w/w) whey protein hydrolysate (WPH) were added to the pasteurized whey. A control with no antioxidant addition was also evaluated. Wheys were stored at 3 °C and evaluated after 0, 2, 4, 6, and 8 d ...


Effects On Lactation Performance When Slick Hair Gene Is Simulated In Dairy Cattle In The Tropics, R. M. Mejía, J. A. Ortuño, G. J. Lascano, M. Vélez Jul 2010

Effects On Lactation Performance When Slick Hair Gene Is Simulated In Dairy Cattle In The Tropics, R. M. Mejía, J. A. Ortuño, G. J. Lascano, M. Vélez

Dairy Science

No abstract provided.


Digestibility Of Fresh Sugarcane-Based Diets With Slow-Release Non Protein Nitrogen Addition For Limit-Fed Dairy Heifers In The Tropics, G. J. Lascano, M. Velez, J. M. Tricarico, A. J. Heinrichs Jul 2010

Digestibility Of Fresh Sugarcane-Based Diets With Slow-Release Non Protein Nitrogen Addition For Limit-Fed Dairy Heifers In The Tropics, G. J. Lascano, M. Velez, J. M. Tricarico, A. J. Heinrichs

Dairy Science

No abstract provided.


Nutrient Utilization Of Different Levels Of Dietary Fiber In Dairy Heifers Limit-Fed High And Low Concentrate Diets, G. J. Lascano, A. J. Heinrichs Jul 2010

Nutrient Utilization Of Different Levels Of Dietary Fiber In Dairy Heifers Limit-Fed High And Low Concentrate Diets, G. J. Lascano, A. J. Heinrichs

Dairy Science

No abstract provided.


Dietary Starch Level And Dose Response Of Saccharomyces Cerevisiae For Limit Fed-Dairy Heifers, G. J. Lascano, J. M. Tricarico, A. J. Heinrichs Jul 2010

Dietary Starch Level And Dose Response Of Saccharomyces Cerevisiae For Limit Fed-Dairy Heifers, G. J. Lascano, J. M. Tricarico, A. J. Heinrichs

Dairy Science

No abstract provided.


Growth Hormone Alters Lipid Composition And Increases The Abundance Of Casein And Lactalbumin Mrna In The Mac-T Cell Line, Tasha L. Johnson, Brent A. S. Fujimoto, Rafael Jiménez-Flores, Daniel G. Peterson May 2010

Growth Hormone Alters Lipid Composition And Increases The Abundance Of Casein And Lactalbumin Mrna In The Mac-T Cell Line, Tasha L. Johnson, Brent A. S. Fujimoto, Rafael Jiménez-Flores, Daniel G. Peterson

Dairy Science

The MAC-T cell line has been used extensively to investigate bovine mammary epithelial cell function. A lactogenic phenotype is generally induced in this cell line by a combination of dexamethasone, insulin and prolactin and has typically been assessed by milk protein production. Few studies have focused on identifying other factors that may affect milk protein synthesis in the MAC-T cell line, and none have considered the lipid class distribution of MAC-T cells as a component of the lactogenic phenotype. Growth hormone (GH) has been shown to increase milk protein synthesis both in vivo and in mammary cell models, and has ...


Lipid Rafts In The Bovine Milk Fat Globule Membrane Revealed By The Lateral Segregation Of Phospholipids And Heterogeneous Distribution Of Glycoproteins, Christelle Lopez, Marie-Noëlle Madec, Rafael Jiménez-Flores May 2010

Lipid Rafts In The Bovine Milk Fat Globule Membrane Revealed By The Lateral Segregation Of Phospholipids And Heterogeneous Distribution Of Glycoproteins, Christelle Lopez, Marie-Noëlle Madec, Rafael Jiménez-Flores

Dairy Science

This study reveals the lateral organisation of the milk fat globule membrane (MFGM). Using confocal laser scanning microscopy (CLSM) and a lipid soluble molecule, an exogenous phospholipid and two lectins as fluorescent probes we located triacylglycerols in the core of fat globules and investigated the organisation of the polar lipids and glycoproteins of the MFGM, in situ in milk. Lipid rafts corresponding to the lateral segregation of sphingolipids in liquid-ordered phases surrounded by liquid-disordered domains composed by the glycerophospholipids were observed in the MFGM. These lipid rafts which correspond to rigid sphingolipid-rich domains have a circular shape at room temperature ...


Culturally Speaking: Beyond Yogurt, Phillip S. Tong May 2010

Culturally Speaking: Beyond Yogurt, Phillip S. Tong

Dairy Science

No abstract provided.


Culturally Speaking: The Year Of Sodium, Phillip S. Tong Feb 2010

Culturally Speaking: The Year Of Sodium, Phillip S. Tong

Dairy Science

No abstract provided.


Investigating The Protective Properties Of Milk Phospholipids Against Ultraviolet Light Exposure In A Skin Equivalent Model, Ashley Russell, Andrea Laubscher, Rafael Jiménez-Flores, Lily H. Laiho Jan 2010

Investigating The Protective Properties Of Milk Phospholipids Against Ultraviolet Light Exposure In A Skin Equivalent Model, Ashley Russell, Andrea Laubscher, Rafael Jiménez-Flores, Lily H. Laiho

Dairy Science

Current research on bioactive molecules in milk has documented health advantages of bovine milk and its components. Milk Phospholipids, selected for this study, represent molecules with great potential benefit in human health and nutrition. In this study we used confocal reflectance and multiphoton microscopy to monitor changes in skin morphology upon skin exposure to ultraviolet light and evaluate the potential of milk phospholipids in preventing photodamage to skin equivalent models. The results suggest that milk phospholipids act upon skin cells in a protective manner against the effect of ultraviolet (UV) radiation. Similar results were obtained from MTT tissue viability assay ...


Concentrate Levels And Saccharomyces Cerevisiae Affect Rumen Fluid-Associated Bacteria Numbers In Dairy Heifers, G. J. Lascano, G. I. Zanton, A. J. Heinrichs Dec 2009

Concentrate Levels And Saccharomyces Cerevisiae Affect Rumen Fluid-Associated Bacteria Numbers In Dairy Heifers, G. J. Lascano, G. I. Zanton, A. J. Heinrichs

Dairy Science

Total viable rumenbacteria counts through the use of colony-unit forming assays lack accuracy because they only include culturable bacteria capable of initiating cell division. Thus, bacterial counts can be underestimated. The use of fluorescent characteristics of cell membranes allows flow cytometry to enumerate and distinguish dead from live bacteria cells. The objective of this experiment was to investigate the viable and total ruminal bacteria counts when 3 levels of forage:concentrate in diets were fed at restricted levels with the addition of Saccharomycescerevisiae (YC). Three cannulated post-pubertal Holstein heifers (age 18 ± 1.0 months) were fed corn silage (CS)-based ...


Culturally Speaking: Ethnic Dairy Foods, Phillip S. Tong Dec 2009

Culturally Speaking: Ethnic Dairy Foods, Phillip S. Tong

Dairy Science

No abstract provided.


Rumen Fermentation Pattern Of Dairy Heifers Fed Restricted Amounts Of Low, Medium, And High Concentrate Diets Without And With Yeast Culture, G. J. Lascano, A. J. Heinrichs Sep 2009

Rumen Fermentation Pattern Of Dairy Heifers Fed Restricted Amounts Of Low, Medium, And High Concentrate Diets Without And With Yeast Culture, G. J. Lascano, A. J. Heinrichs

Dairy Science

Restricted feeding and highconcentratediets are potential strategies for growing dairyheifers. Ruminal manipulation with additives such as Saccharomyces cerevisiaeyeastculture (YC) has been shown to alter digestibility when added to this type of diet. An experiment was conducted to investigate the ruminal fermentation and in situ digestibility of diets with 3 different levels of forage to concentrate (F:C) fed at restricted intake without and with YC addition. Three cannulated post-pubertal Holstein heifers (age 18.0 ± 1.2 months; body weight 449.6 ± 19.7 kg) were feddiets consisting of corn silage as the sole forage source in a 3 period ...


Phil Tong On Nutritious Frozen Dairy, Phillip S. Tong Sep 2009

Phil Tong On Nutritious Frozen Dairy, Phillip S. Tong

Dairy Science

No abstract provided.


Culturally Speaking: Shelf Life, Phillip S. Tong Jun 2009

Culturally Speaking: Shelf Life, Phillip S. Tong

Dairy Science

No abstract provided.


Culturally Speaking: Raw Milk Quality, Phillip S, Tong Apr 2009

Culturally Speaking: Raw Milk Quality, Phillip S, Tong

Dairy Science

No abstract provided.


Ammonia Emissions And Olfactometry Analysis Of Limit Fed High And Low Concentrate Diets With Different Forage Quality In Dairy Heifers, G. J. Lascano, P. A. Topper, A. Adviento-Borbe, D. Topper, R. C. Brandt, E. F. Wheeler, A. J. Heinrichs Jul 2008

Ammonia Emissions And Olfactometry Analysis Of Limit Fed High And Low Concentrate Diets With Different Forage Quality In Dairy Heifers, G. J. Lascano, P. A. Topper, A. Adviento-Borbe, D. Topper, R. C. Brandt, E. F. Wheeler, A. J. Heinrichs

Dairy Science

No abstract provided.


The Use Of Flow Cytometry To Assess Rumen Bacteria In Dairy Heifers Limit Fed Different Forage To Concentrate Ratios With Saccharomyces Cerevisiae, G. J. Lascano, A. J. Heinrichs Jul 2008

The Use Of Flow Cytometry To Assess Rumen Bacteria In Dairy Heifers Limit Fed Different Forage To Concentrate Ratios With Saccharomyces Cerevisiae, G. J. Lascano, A. J. Heinrichs

Dairy Science

No abstract provided.


Odor Evaluation And Gas Emission From Manure Of Dairy Heifers Fed High/Low - Forage Quality And High/Low-Concentrate Diets, Patrick A. Topper, Robin Brandt, Arlene Adviento-Borbe, Gustavo J. Lascano, Arlyn J. Heinrichs, Eileen F. Wheeler Jun 2008

Odor Evaluation And Gas Emission From Manure Of Dairy Heifers Fed High/Low - Forage Quality And High/Low-Concentrate Diets, Patrick A. Topper, Robin Brandt, Arlene Adviento-Borbe, Gustavo J. Lascano, Arlyn J. Heinrichs, Eileen F. Wheeler

Dairy Science

A heifer feeding trial evaluated the impact of high/low forage quality and high/low concentrate level nutrient-balanced diets on simultaneous odor and gas emissions from the manure. Gas concentration was determined using an infrared photoacoustic analyzer over a 24-hour period using a steady-state flux chamber setup containing urine:feces as-excreted from eight individual heifers. Odorous air samples were collected from chamber headspace and evaluated by six human assessors for pleasantness, intensity and detection threshold using a forced-choice dynamic olfactometer. Ammonia emission ranged from 0.64 to 3.94 mg NH3 cm-2 d-1 across diets. Average ammonia emission from the ...


Staying Alive: Maximizing Live Bacteria In Fermented Products, Phillip S. Tong Feb 2008

Staying Alive: Maximizing Live Bacteria In Fermented Products, Phillip S. Tong

Dairy Science

No abstract provided.


The Milk Fat Globule Membrane As An Ingredient: Why, How, When?, Rafael Jiménez-Flores, Guillaume Brisson Jan 2008

The Milk Fat Globule Membrane As An Ingredient: Why, How, When?, Rafael Jiménez-Flores, Guillaume Brisson

Dairy Science

This paper presents a personal view on the potential applications of the milk fat globule membrane as an ingredient in the processed foods area. Several factors are of importance for this dissertation: the biological origin of the membrane, the voluminous literature on its individual components and their relationship with health and wellness, the biological role of milk in nutrition to mammals and the innovation on scientific tools being applied in many fields of chemistry and biology. We hope to give a glimpse of the reasons on why it is a good idea to use more efficiently the components of the ...


Foreword, 3rd International Spray Dried Milk Products Conference Proceedings, Phillip S. Tong Jan 2008

Foreword, 3rd International Spray Dried Milk Products Conference Proceedings, Phillip S. Tong

Dairy Science

No abstract provided.


Impact Of Protein Standardization Of Milk Powder With Lactose Or Pemeate On Whey Protein Nitrogen Index And Heat Classification, Vandna Sikand, Phillip S. Tong, John Walker Jan 2008

Impact Of Protein Standardization Of Milk Powder With Lactose Or Pemeate On Whey Protein Nitrogen Index And Heat Classification, Vandna Sikand, Phillip S. Tong, John Walker

Dairy Science

No abstract provided.


Effect Of Processing On The Composition And Microstructure Of Buttermilk And Its Milk Fat Globule Membranes, Pierre Morin, Rafael Jiménez-Flores, Yves Pouliot Oct 2007

Effect Of Processing On The Composition And Microstructure Of Buttermilk And Its Milk Fat Globule Membranes, Pierre Morin, Rafael Jiménez-Flores, Yves Pouliot

Dairy Science

The effect of cream pasteurization on the composition and microstructure of buttermilk after pasteurization, evaporation and spray-drying was studied. The composition of milk fat globule membrane (MFGM) isolated from buttermilk samples was also characterized. Pasteurization of cream resulted in higher lipid recovery in the buttermilk. Spray-drying of buttermilk had a significant effect on phospholipid content and composition. After spray-drying, the phospholipid content decreased by 38.2% and 40.6%, respectively in buttermilk from raw or pasteurized cream when compared with initial buttermilks. Pasteurization of cream resulted in the highest increase in whey protein recovery in MFGM isolates compared with all ...


Rumen Fermentation Patterns Of Dairy Heifers Fed Restricted Amounts Of High, Medium, And Low Concentrate Diets And The Addition Of Saccharomyces Cerevisiae, G. J. Lascano, A. J. Heinrichs Jul 2007

Rumen Fermentation Patterns Of Dairy Heifers Fed Restricted Amounts Of High, Medium, And Low Concentrate Diets And The Addition Of Saccharomyces Cerevisiae, G. J. Lascano, A. J. Heinrichs

Dairy Science

No abstract provided.


Digestibility Of Limit Fed High And Low Concentrate Diets With Corn Silage As The Sole Forage For Dairy Heifers With Saccharomyces Cerevisiae, G. J. Lascano, A. J. Heinrichs Jul 2007

Digestibility Of Limit Fed High And Low Concentrate Diets With Corn Silage As The Sole Forage For Dairy Heifers With Saccharomyces Cerevisiae, G. J. Lascano, A. J. Heinrichs

Dairy Science

No abstract provided.