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Fermentation

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Full-Text Articles in Life Sciences

Comparative Analysis Of Metabolic Pathways Of Bacteria Used In Fermented Food, Keanu Hoang, Kiran Bastola May 2020

Comparative Analysis Of Metabolic Pathways Of Bacteria Used In Fermented Food, Keanu Hoang, Kiran Bastola

Theses/Capstones/Creative Projects

This study presents a novel methodology for analyzing metabolic pathways. Utilizing KEGG REST API through a Biopython package and file parser, data about whether or not a bacteria has an enzyme or not was extracted. The results found that differences in metabolic pathway enrichment values follow along the lines of genera and pathway type. In particular, bacteria found in food spoilage and commercial nitrogen fixing products had high values of enrichment.


The Effect Of Different Types Of Inoculants On The Characteristics Of Alfalfa, Ryegrass And Red Clover-Ryegrass-Timothy Silage, Vilma Vrotniakiene, Jonas Jatkauskas Mar 2020

The Effect Of Different Types Of Inoculants On The Characteristics Of Alfalfa, Ryegrass And Red Clover-Ryegrass-Timothy Silage, Vilma Vrotniakiene, Jonas Jatkauskas

International Grassland Congress Proceedings

Silage inoculants based on lactic acid bacteria (LAB) have demonstrated improvements in energy and nutrient recovery from grass and legume silages by lowering the pH and shifting the fermentation towards lactic acid production, by reducing storage losses due to spoilage and by increasing the aerobic stability during feed-out. There are different mixtures of strains currently on the market.

The present study was conducted according to the Deutsche Landwirtschafts-Gesellschaft Guidelines for the testing of silage additives in order to assess which mixtures of lactic acid bacteria would have a greater potential to improve fermentation profile of alfalfa, perennial ryegrass and red ...


Effects Of Different Feed Additives On Methane Emissions From Beef Cattle, P. Giraldo, A. Marín, L. A. Giraldo Feb 2020

Effects Of Different Feed Additives On Methane Emissions From Beef Cattle, P. Giraldo, A. Marín, L. A. Giraldo

International Grassland Congress Proceedings

The rate of accumulation of methane in the atmosphere from enteric fermentation in cattle has an important impact on the greenhouse effect and contributing to global warming. Additionally, methane emission reduces the energy efficiency of substrate fermention in the rumen. Under-standing the effect of the diet on enteric methane emissions could help to identify strategies to reduce emissions of this greenhouse gas. Therefore, the main objective of the present investigation was to determine the effect of nutritional additives such as monensin, fumaric acid, tannins of Acacia decurrens, and glycerol on methane production and other measures of fermentation characteristics using the ...


Fermentation Parameters Of Kikuyu Grass (Pennisetum Clandestinum) By In Vitro Gas Production Technique (Ivgpt), A. Marín, P. Giraldo, L. A. Giraldo Feb 2020

Fermentation Parameters Of Kikuyu Grass (Pennisetum Clandestinum) By In Vitro Gas Production Technique (Ivgpt), A. Marín, P. Giraldo, L. A. Giraldo

International Grassland Congress Proceedings

Methane (CH4) is a byproduct of ruminal fermentation whose production is directly related to forage quality, which in turn is affected by a myriad of environmental factors. In general the quality of pastures in the tropics range from medium to poor due to a high content of lignocellulosic material of low digestibility and a low content of other components, such as soluble carbohydrates and protein (Correa et al, 2008). CH4 produced by enteric fermentation from cattle rumen represents a major source of greenhouse gases (GHG). By measuring these emissions the impact of dairy production systems on the climate ...


Hedges And Woody Strips Browsing By Cattle On Pasture In Wallonia, Belgium, Sophie Vandermeulen, Mathilde Pascal, Christian Marche, Arnaud Pilet, Yves Beckers, Jérôme Bindelle Feb 2020

Hedges And Woody Strips Browsing By Cattle On Pasture In Wallonia, Belgium, Sophie Vandermeulen, Mathilde Pascal, Christian Marche, Arnaud Pilet, Yves Beckers, Jérôme Bindelle

International Grassland Congress Proceedings

Shrub and tree forages are commonly used in animal production in many regions of the world. Nonetheless, in Western Europe, and especially in Wallonia in Belgium, hedges and woody strips have disappeared from the agricultural landscape over the past 60 years. Browse species are usually rich in plant secondary compounds such as tannins whose benefits on CH4 production and intestinal parasitism have been highlighted (Ramírez-Restrepo et al. 2010). Currently, agri-environmental measures taken by the Walloon government promote hedges and woody strips in pastures, raising the interest in browse species functionalities in ruminant production.

This study aimed at: (1) determining the ...


Utilization Of Mechanocatalytic Oligosaccharides By Ethanologenic Escherichia Coli As A Model Microbial Cell Factory, Tao Jin, Mats Käldström, Adriana Benavides, Marcelo D. Kaufman Rechulski, Laura R. Jarboe Feb 2020

Utilization Of Mechanocatalytic Oligosaccharides By Ethanologenic Escherichia Coli As A Model Microbial Cell Factory, Tao Jin, Mats Käldström, Adriana Benavides, Marcelo D. Kaufman Rechulski, Laura R. Jarboe

Chemical and Biological Engineering Publications

Mechanocatalysis is a promising method for depolymerization of lignocellulosic biomass. Microbial utilization of the resulting oligosaccharides is one potential route of adding value to the depolymerized biomass. However, it is unclear how readily these oligosaccharides are utilized by standard cell factories. Here, we investigate utilization of cellulose subjected to mechanocatalytic depolymerization, using ethanologenic Escherichia coli as a model fermentation organism. The mechanocatalytic oligosaccharides supported ethanol titers similar to those observed when glucose was provided at comparable concentrations. Tracking of the various oligomers, using maltose (alpha-1,4) and cellobiose (beta-1,4) oligomers as representative standards of the orientation, but not linkage ...


Changes To The Fermentative Profiles Of Pioneiro Grass (Pennisetum Purpureum Schumach) And Maize Silages In Different Mixtures After Aerobic Stability, Eduardo R. Scherer, Tiago M. Santos, Diana R. Vivian, José A. Freitas, Kátia F. Gobbi, Américo F. Garcez Neto Jan 2020

Changes To The Fermentative Profiles Of Pioneiro Grass (Pennisetum Purpureum Schumach) And Maize Silages In Different Mixtures After Aerobic Stability, Eduardo R. Scherer, Tiago M. Santos, Diana R. Vivian, José A. Freitas, Kátia F. Gobbi, Américo F. Garcez Neto

International Grassland Congress Proceedings

Silages arte widely used in animal production systems to overcome the seasonality of forage production around the world. The preservation of nutritional value of silages depends on the maintenance of an anaerobic environment during the fermentation and storage phases, as well from aerobic stability during the feed out stage (Nussio, 2005). Aerobic exposure usually promotes intense activity of fungi, yeast and aerobic bacteria. If the time of aerobic exposure is long enough, huge changes can happen in the chemical composition of the silages affecting negatively their nutritional value. These changes can be followed by decreasing lactic acid concentration and increasing ...


Silage Inoculants Improves Quality And Aerobic Stability In Grass, Clover-Grass And Lucerne Silage, Jonas Jatkauskas, Vilma Vrotniakiene, Christer Ohlsson, Bente Lund Jan 2020

Silage Inoculants Improves Quality And Aerobic Stability In Grass, Clover-Grass And Lucerne Silage, Jonas Jatkauskas, Vilma Vrotniakiene, Christer Ohlsson, Bente Lund

International Grassland Congress Proceedings

The type of silage additive will influence the characteristics of fermentation, dry matter (DM) losses, hygienic quality and aerobic stability of silage. The appropriate use of an additive also depends on the crop to which it is applied. Homofermentative lactic acid bacteria (LAB) strains are selected for rapid growth under under variable temperatures, herbages and dry matter concentrations. The homofermentative LAB are highly competitive and produce largely lactic acid and reduce pH and ammonia-N content compared to untreated silage (Muck 2012). Heterofermentative LAB such as Lactobacillus buchneri reduce the growth of yeast and mould and increase aerobic stability. These effects ...


The Exploration Of Nanotoxicological Copper And Interspecific Saccharomyces Hybrids, Matthew Joseph Winans Phd Jan 2020

The Exploration Of Nanotoxicological Copper And Interspecific Saccharomyces Hybrids, Matthew Joseph Winans Phd

Graduate Theses, Dissertations, and Problem Reports

Nanotechnology takes advantage of cellular biology’s natural nanoscale operations by interacting with biomolecules differently than soluble or bulk materials, often altering normal cellular processes such as metabolism or growth. To gain a better understanding of how copper nanoparticles hybridized on cellulose fibers called carboxymethyl cellulose (CMC) affected growth of Saccharomyces cerevisiae, the mechanisms of toxicity were explored. Multiple methodologies covering genetics, proteomics, metallomics, and metabolomics were used during this investigation. The work that lead to this dissertation discovered that these cellulosic copper nanoparticles had a unique toxicity compared to copper. Further investigation suggested a possible ionic or molecular mimicry ...


Validation Of A Fluorescence-Based Bacteria Enumeration And Viability Determination Method Using Image Cytometry, Matthew R. Hodgkin Dec 2019

Validation Of A Fluorescence-Based Bacteria Enumeration And Viability Determination Method Using Image Cytometry, Matthew R. Hodgkin

Electronic Theses and Dissertations

Craft beer production in the United States has been increasing significantly in recent years. As production and participation in the industry increases, so have consumer demands for more beer options. Breweries have met this call with experimentation of ingredients in their beer and also the styles they brew. One such beer, the sour beer, is an example of this experimentation and new product development. Sour beers are a style that differ from most other beers by how they are manufactured. Instead of being fermented with only yeast, both a lactic acid-producing bacteria and yeast are used. With increased competition amongst ...


Effect Of Processing On Microbiota Accessible Carbohydrates In Whole Grains, Caroline Smith Nov 2019

Effect Of Processing On Microbiota Accessible Carbohydrates In Whole Grains, Caroline Smith

Dissertations, Theses, & Student Research in Food Science and Technology

There is potential to increase microbiota accessible carbohydrates (MAC) in whole grains through food processing. Therefore, different processing conditions for boiling and extrusion of whole wheat flour were studied for their effect on MAC. Processing conditions had a minimal effect on fermentation response using 2 fecal samples of disparate composition. However, the processing method had an impact on the fermentation of non-digestible carbohydrates and subsequent short chain fatty acids production, but only for a microbiome with high diversity and a predominance of microbes associated with dietary fiber intake. Subsequently, five processing methods: boiling, extrusion, unleavened bread, yeast bread, and sourdough ...


A Comparative Analysis Of The Fermentation Capabilities Of Various Bifidobacterium Strains, Ella Oney Mar 2019

A Comparative Analysis Of The Fermentation Capabilities Of Various Bifidobacterium Strains, Ella Oney

Honors Theses, University of Nebraska-Lincoln

Bifidobacterium is a genus of anaerobic bacteria that are commonly found to inhabit the gastrointestinal tract of many members of the animal kingdom. These microorganisms are adapted to obtain their carbon from the breakdown of complex carbohydrates. Marmosets, a mammal whose gut microbiome is inhabited by high levels of Bifidobacteria, consume gum Arabic as a major part of their diet. The purpose of this experiment is to determine whether Bifidobacterium strains isolated from the guts of marmosets are able to degrade and ferment this complex carbohydrate or one of its main constituents, arabinose. This was accomplished by inoculating isolates of ...


The Evaluation Of Pathogen Survival In Dry Cured Charcuterie Style Sausages, Jennifer Michelle Mcneil Jan 2019

The Evaluation Of Pathogen Survival In Dry Cured Charcuterie Style Sausages, Jennifer Michelle Mcneil

Theses and Dissertations--Animal and Food Sciences

The objective of this study was to evaluate the survival of non-O157:H7 STEC, Salmonella spp., and S. aureus in dry fermented sausages. Chorizo and Landjager sausages were inoculated with individual bacterial cocktails and stuffed into natural casings. Temperature, relative humidity, pH, and water activity were monitored through fermentation, drying, and storage. Bacterial counts were determined by serial dilution and plated in triplicates on selective media. Plates were incubated at 37°C for 24 hours and colony forming units per gram (CFU/g) were observed.

Results of the first study validate that contaminated raw materials contribute to pathogen survival and ...


Twin-Screw Extrusion Processing Of Plant-Based Blends Using Graded Levels Of High Protein Fermented Soybean Meal (Fsbm), Parisa Fallahi, Kurt A. Rosentrater, K. Muthukumarappan Jan 2019

Twin-Screw Extrusion Processing Of Plant-Based Blends Using Graded Levels Of High Protein Fermented Soybean Meal (Fsbm), Parisa Fallahi, Kurt A. Rosentrater, K. Muthukumarappan

Agricultural and Biosystems Engineering Publications

Fast-paced growth in global aquaculture has elevated concerns about the high costs of fish farming production and potential water pollution. Thus, finding eco-friendly and more sustainable alternative protein sources for fish diets is of vital importance to the industry. A twin-screw extrusion processing study was performed using three ingredient blends formulated with graded levels of high protein fermented soybean meal (FSBM) (0, 80% and 100% db fishmeal replacement) along with calculated amounts of other ingredients to meet the rainbow trout diets’ requirements. Increasing the FSBM content from 0% to 100% resulted in a substantial increase in brightness, greenness, and yellowness ...


Ethanol, W. J. Criddle, Jacek A. Koziel, J. (Hans) Van Leeuwen, William S. Jenks Jan 2019

Ethanol, W. J. Criddle, Jacek A. Koziel, J. (Hans) Van Leeuwen, William S. Jenks

Agricultural and Biosystems Engineering Publications

Ethanol (CH3CH2OH) is one of the most important individual organic compounds readily available all over the world. It has been described as one of the most versatile of compounds, being widely used as a solvent, a germicide, a beverage, an antifreeze, a fuel, and fuel additive, additive to paints, coatings, automotive care products and adhesives, a fragrance, a lubricant, and as an intermediate for the synthesis and production of other organic compounds. Ethanol is mainly produced through yeast fermentation of sugars from seeds, sugar cane or beets and concentrated through subsequent distillation. Its main use is as a liquid fuel ...


Estimating Dry Matter Digestibility Of Forage In Equine Diets, Taylor N. Godwin Jan 2019

Estimating Dry Matter Digestibility Of Forage In Equine Diets, Taylor N. Godwin

MSU Graduate Theses

Previous research indicates equine fecal inoculates produce comparable results to cecal fluid when used for in vitro procedures to analyze dry matter digestibility (DMD). Equine hindgut microbial populations represented in fecal samples have been shown to be affected by diet. The objective of this study was to determine the effect of diet on in vitro DMD when fecal samples from horses on varying starch and fiber diets were used as inoculates. Six mature Quarter Horses (BW 522 ± 45kg) were used in a crossover repeated measures design to compare the effects of a grain vs. all forage diets on the in ...


Evaluation Of Fermentation At 40°C And 30°C For Cost Effective Lignocellulose To Lipid Conversion, Kyle M. Macewen Dec 2018

Evaluation Of Fermentation At 40°C And 30°C For Cost Effective Lignocellulose To Lipid Conversion, Kyle M. Macewen

Graduate Masters Theses

As the world population continues to grow, the demand for energy will continue to rise. Biofuels have become an attractive alternative to replace fossil fuels as a clean and renewable source of energy. The six- and five-carbon sugars contained in lignocellulosic plant biomass is the largest carbohydrate source in the world, and a key feedstock for sustainable biofuel production. The conversion of lignocellulose to lipids is done by using oleaginous yeast as a biocatalyst. Recently, Arxula adeninivorans has become a yeast of interest because of its unique properties. These include its unusual metabolic flexibility which allows it to utilize a ...


Impact Of Temperature On Beer Fermentation Kinetics, Hasime Manaj, Luljeta Pinguli, Ilirjan Malollari, Anisa Dhroso Oct 2018

Impact Of Temperature On Beer Fermentation Kinetics, Hasime Manaj, Luljeta Pinguli, Ilirjan Malollari, Anisa Dhroso

UBT International Conference

Microbial processes are highly dependent on temperature. This paper aims to study the influence of temperature on fermentation process on experimental and industrial scale. We were focused to determine the pre- exponential coefficients and the activation energy for experimental and industrial conditions and to quantify the amount of heat to be removed from fermenter to keep it under constant temperature during fermentation process. Substrate used for fermentation was beer industrial wort 120 Balling and yeast strain used was bottom fermentation yeast "Saccharomyces Carlsbegensis SP 35". Evaluation and comparison of kinetic parameters in different fermentation conditions were carried out. Temperatures used ...


Genau, Charles (Fa1212), Manuscripts & Folklife Archives Oct 2018

Genau, Charles (Fa1212), Manuscripts & Folklife Archives

FA Finding Aids

Finding aid only for Folklife Archives Project 1212. Student paper titled “Winemaking: The Old and New Way” in which Charles Genau details the process of wine production from harvest, to fermentation, bottling, and distribution. Genau compares and contrasts methods of traditional winemaking with contemporary approaches. He also gathered his information from two winemakers of different ages and ethnic backgrounds. The paper contains two illustrations of the winemaking process.


Isolation, Purification And Characterization Of A Novel Solvent Stable Lipase From Pseudomonas Reinekei, Barry Ryan, Priyanka Priyanka, Gemma K. Kinsella, Gary T. Henehan Sep 2018

Isolation, Purification And Characterization Of A Novel Solvent Stable Lipase From Pseudomonas Reinekei, Barry Ryan, Priyanka Priyanka, Gemma K. Kinsella, Gary T. Henehan

Articles

The Pseudomonas sp. have been long recognized for their exogenous lipolytic activities yet the genus still contains a lot of unexplored strains. Due to the versatile metabolic machinery and their potential for adaptation to fluctuating environmental conditions Pseudomonas sp. are of great interest for biotechnological applications. In this study, a new extracellularly produced lipolytic enzyme from Pseudomonas sp. (P. reinekei) was purified and characterized. The production of lipase from P. reinekei (H1) was enhanced 10-fold by optimizing the nitrogen source. The 50 kDa H1 lipase was purified using negative and positive mode anion exchange chromatography. The purified lipase was active ...


Development Of Kefir Products Using Aronia Or Elderberries And The Impacts Of Fermentation On The Health-Promoting Characteristics Of Aronia Polyphenols, Xue Du Aug 2018

Development Of Kefir Products Using Aronia Or Elderberries And The Impacts Of Fermentation On The Health-Promoting Characteristics Of Aronia Polyphenols, Xue Du

Electronic Theses and Dissertations

Diabetes is a global health problem. The consumption of dietary polyphenols may help to decrease the risk of type 2 diabetes and slow the progression of diabetic complications. Aronia (Aronia melanocarpa) and elderberry (Sambucus nigra L. ssp. canadensis) fruits are rich in polyphenols and exhibit health-promoting properties, but they are underutilized. Aronia and elderberries are rarely consumed raw due to the astringent mouth feel. New food products are needed to increase their consumption. Kefir, a fermented dairy beverage, was chosen to be the matrix for incorporating berries due to: 1) the protein matrix can help mask the astringency; 2) an ...


Development And Shelf Life Evaluation Of A Novel Fermented Seaweed Sauerkraut Utilizing Commercially Important Maine Seaweeds, Sarah M. Brochu Aug 2018

Development And Shelf Life Evaluation Of A Novel Fermented Seaweed Sauerkraut Utilizing Commercially Important Maine Seaweeds, Sarah M. Brochu

Electronic Theses and Dissertations

Fermented vegetables can provide consumers with important health benefits, particularly due to the presence of probiotics. These fermented products have gained popularity with American consumers over the past decade. Therefore, a lacto-fermented seaweed sauerkraut, containing seaweed and cabbage, was developed to address this trend and to create a value-added seaweed product with extended refrigerated shelf life. The objective of this study was to evaluate the effects of kelp species and seaweed incorporation level on the fermentative success, microbial safety, consumer acceptability, and refrigerated shelf life of seaweed sauerkraut for 60 days post inoculation.

Six treatments with varying levels (25%, 50 ...


Autohydrolysis Pretreatment Of Mixed Lignocellulosic Biomass, Claudio Lira Jun 2018

Autohydrolysis Pretreatment Of Mixed Lignocellulosic Biomass, Claudio Lira

Electronic Thesis and Dissertation Repository

Lignocellulosic biomass, such as agricultural and forestry residues, can be transformed into valuable fuels and chemicals. However, the high cost of feedstock handling and the complex nature of cellulosic biomass processing and conversion remain two major challenges in biorefineries. This thesis aims to tackle these challenges by assessing the transformation of multi-feedstock using autohydrolysis pretreatment.

Corn cobs and stover (residues from corn processing), poplar chips (forestry residue), bagasse and wheat straw (agricultural residues) are abundant and often under-utilized cellulosic residues. These feedstocks were used to investigate the effects of biomass characteristics on the composition of the autohydrolysis pretreatment products. Moreover ...


Kinetic Modeling Of Corn Fermentation With S. Cerevisiae Using A Variable Temperature Strategy, Augusto C. M. Souza, Mohammad Mousaviraad, Kenneth O. M. Mapoka, Kurt A. Rosentrater Apr 2018

Kinetic Modeling Of Corn Fermentation With S. Cerevisiae Using A Variable Temperature Strategy, Augusto C. M. Souza, Mohammad Mousaviraad, Kenneth O. M. Mapoka, Kurt A. Rosentrater

Agricultural and Biosystems Engineering Publications

While fermentation is usually done at a fixed temperature, in this study, the effect of having a controlled variable temperature was analyzed. A nonlinear system was used to model batch ethanol fermentation, using corn as substrate and the yeast Saccharomyces cerevisiae, at five different fixed and controlled variable temperatures. The lower temperatures presented higher ethanol yields but took a longer time to reach equilibrium. Higher temperatures had higher initial growth rates, but the decay of yeast cells was faster compared to the lower temperatures. However, in a controlled variable temperature model, the temperature decreased with time with the initial value ...


The Use Of Grape By-Products As A Nutrient Rich Cattle Feed, Zachary Christman Mar 2018

The Use Of Grape By-Products As A Nutrient Rich Cattle Feed, Zachary Christman

Theses, Dissertations, and Student Research in Agronomy and Horticulture

In this article you will learn about how to use the grape stems, skins and pulp that are generated by the wine industry. Ruminant animals such as cattle can digest this inexpensive yet nutrient rich material. The complete process from wine press to cattle feed is covered in this article. The historical background of using grape by-products and methods to preserve this food source is also presented.


Informing Efforts To Develop Nitroreductase For Amine Production, Anne-Frances Miller, Jonathan T. Park, Kyle L. Ferguson, Warintra Pitsawong, Andreas S. Bommarius Jan 2018

Informing Efforts To Develop Nitroreductase For Amine Production, Anne-Frances Miller, Jonathan T. Park, Kyle L. Ferguson, Warintra Pitsawong, Andreas S. Bommarius

Chemistry Faculty Publications

Nitroreductases (NRs) hold promise for converting nitroaromatics to aromatic amines. Nitroaromatic reduction rate increases with Hammett substituent constant for NRs from two different subgroups, confirming substrate identity as a key determinant of reactivity. Amine yields were low, but compounds yielding amines tend to have a large π system and electron withdrawing substituents. Therefore, we also assessed the prospects of varying the enzyme. Several different subgroups of NRs include members able to produce aromatic amines. Comparison of four NR subgroups shows that they provide contrasting substrate binding cavities with distinct constraints on substrate position relative to the flavin. The unique architecture ...


Ffa Generation During Dry-Grind Corn Ethanol Fermentation, Brett Brothers Jan 2018

Ffa Generation During Dry-Grind Corn Ethanol Fermentation, Brett Brothers

Graduate Theses and Dissertations

The fermentation of corn into ethanol is a major usage of the American-grown corn crop. During the process of corn to ethanol, corn lipid class composition changes. The formation of free fatty acid (FFA) from triacylglycerol is commonly observed. Increased free fatty acid fermentation byproducts result in troublesome processing to remove excess FFA. The objective of this work was to determine where in the process of corn to ethanol FFA is generated using both industrial and laboratory-scale approaches. Specific commercial steps and conditions in the process of corn to ethanol were tested to determine their role in the generation of ...


Food-Derived Bioactive Peptides In Human Health: Challenges And Opportunities, Subhadeep Chakrabarti, Snigdha Guha, Kaustav Majumder Jan 2018

Food-Derived Bioactive Peptides In Human Health: Challenges And Opportunities, Subhadeep Chakrabarti, Snigdha Guha, Kaustav Majumder

Faculty Publications in Food Science and Technology

Recent scientific evidence suggests that food proteins not only serve as nutrients, but can also modulate the body’s physiological functions. These physiological functions are primarily regulated by some peptides that are encrypted in the native protein sequences. These bioactive peptides can exert health beneficial properties and thus are considered as a lead compound for the development of nutraceuticals or functional foods. In the past fewdecades, awide range of food-derived bioactive peptide sequences have been identified, with multiple health beneficial activities. However, the commercial application of these bioactive peptides has been delayed because of the absence of appropriate and scalable ...


Transcriptional Profiling Of Saccharomyces Cerevisiae Reveals The Impact Of Variation Of A Single Transcription Factor On Differential Gene Expression In 4nqo, Fermentable, And Nonfermentable Carbon Sources, Xiaoqing Rong-Mullins, Michael C. Ayers, Mahmoud Summers, Jennifer E.G. Gallagher Jan 2018

Transcriptional Profiling Of Saccharomyces Cerevisiae Reveals The Impact Of Variation Of A Single Transcription Factor On Differential Gene Expression In 4nqo, Fermentable, And Nonfermentable Carbon Sources, Xiaoqing Rong-Mullins, Michael C. Ayers, Mahmoud Summers, Jennifer E.G. Gallagher

Faculty & Staff Scholarship

Cellular metabolism can change the potency of a chemical’s tumorigenicity. 4-nitroquinoline-1-oxide (4NQO) is a tumorigenic drug widely used on animal models for cancer research. Polymorphisms of the transcription factor Yrr1 confer different levels of resistance to 4NQO in Saccharomyces cerevisiae. To study how different Yrr1 alleles regulate gene expression leading to resistance, transcriptomes of three isogenic S. cerevisiae strains carrying different Yrr1 alleles were profiled via RNA sequencing (RNA-Seq) and chromatin immunoprecipitation coupled with sequencing (ChIP-Seq) in the presence and absence of 4NQO. In response to 4NQO, all alleles of Yrr1 drove the expression of SNQ2 (a multidrug transporter ...


Improving The Nutritional Characteristics Of Plant Feedstuff By-Products Using Fungal Metabolism, Jacob Zahler Jan 2018

Improving The Nutritional Characteristics Of Plant Feedstuff By-Products Using Fungal Metabolism, Jacob Zahler

Electronic Theses and Dissertations

Plant feedstuff by-products such as soy processing wastewater, guar korma meal, and sorghum hominy are very different, however, all possess a rich nutrient profile. Lessening their value is the presence of lignocellulose, plant anti-nutritional factors, and unfavorable protein profiles. Fungal conversion processes are an attractive approach to improving the value of these by-products by degrading detrimental fractions of each while simultaneously creating nutrient-rich cell mass. The aim of this research was to improve the value of each by-product for potential application in monogastric diets such as fish. Fungal organisms, both yeast and filamentous fungi, were examined for their ability to ...