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Full-Text Articles in Life Sciences

Native Deer Mice (Peromyscus Maniculatus) Use Of Invasive Grass Seed (Ehrharta Calycina) In Coastal California, Megan Wong Dec 2015

Native Deer Mice (Peromyscus Maniculatus) Use Of Invasive Grass Seed (Ehrharta Calycina) In Coastal California, Megan Wong

Biological Sciences

No abstract provided.


Developing A Novel Means Of Observing The Intracellular Activities Of Heterogeneous Nuclear Ribonucleoprotein K Within Xenopus Laevis, Janeah A. Alexis May 2015

Developing A Novel Means Of Observing The Intracellular Activities Of Heterogeneous Nuclear Ribonucleoprotein K Within Xenopus Laevis, Janeah A. Alexis

Biological Sciences

Research into the regeneration of optic nerves in Xenopus laevis has determined that heterogeneous nuclear ribonucleoprotein K (hnRNP K) plays a crucial role in regulating the trafficking and translation of mRNAs essential for the organization of the axonal cytoskeleton. To further explore this role, our lab has turned to tools that can definitively elucidate hnRNP K’s translocation in-and-out of the nucleus, as well as directly quantitate its degradation rate, in vivo. An appropriate tool for such experiments is the monomeric Eos fluorescent protein (mEosFP), which can be stably and irreversibly photo-converted. This fluorescent protein naturally emits green light (~516nm ...


Use Of A Ph-Sensitive Probe To Study The Role Of Pkc-Epsilon In Phagocytosis Of Pathogens, Rebekah Marie Pierce May 2015

Use Of A Ph-Sensitive Probe To Study The Role Of Pkc-Epsilon In Phagocytosis Of Pathogens, Rebekah Marie Pierce

Biological Sciences

The main force behind innate immunity, our primary defense against infection, is the clearance of pathogens, or phagocytosis. Protein Kinase C-epsilon (PKC-ε) is necessary for efficient phagocytosis of antibody (IgG)-coated particles. Previous studies used IgG-coated glass beads, which are larger and more rigid than bacteria and lack their complex surface protein expression. To test the hypothesis that PKC-ε is necessary for phagocytosis of pathogens, we compared the rate of uptake of common pathogens by macrophages from wild type and PKC- ε knock out mice. We tested two targets, a bacteria (E. coli) and a yeast (zymosan), both physiologically relevant ...


Structure-Activity Relationship Studies Of Small Molecules Directed Against The T-Box Specifier Loop, Spencer Weintraub May 2015

Structure-Activity Relationship Studies Of Small Molecules Directed Against The T-Box Specifier Loop, Spencer Weintraub

Biological Sciences

High rates of antibiotic use have resulted in the pervasiveness of multi-drug resistant organisms. With rising drug-resistance the development of antibiotics with new targets against these pathogens is imperative. The T-box regulatory mechanism is a process specific to Grampositive bacteria and controls many essential genes including aaRS genes, which are code for the aminoacyl-tRNA synthetases required to charge tRNA. The T-box Specifier Loop is a novel target for antibacterial drug discovery as we hypothesize that a small compound bound to the Specifier Loop will inhibit transcription of essential bacterial genes resulting in bacterial cell death or growth arrest. We used ...


Identifying Genes That Affect Lactococcus Lactis Intron Mobility, Mary Njie May 2015

Identifying Genes That Affect Lactococcus Lactis Intron Mobility, Mary Njie

Biological Sciences

A gene consists of coding segments, exons, which are maintained and contribute to the final mRNA product, while non-coding segments, introns, are spliced out through RNA splicing mechanisms to code a functional gene. The mobility of the spliced introns and factors that affect mobility are an important field of study evolutionarily whereas splicing defects have medical implications. For this study, the genome of the Lactococcus lactis bacterial strain, IL1403 and the group II intron of interest, L1.LtrB were utilized. This intron moves into new sites on DNA by a process termed retrotransposition (RTP). Our goal is to identify genes ...


Chronic Resveratrol Treatment Ameliorates Cognitive Deficits Associated With A High Fat Diet, Kelsey E. O'Leary May 2015

Chronic Resveratrol Treatment Ameliorates Cognitive Deficits Associated With A High Fat Diet, Kelsey E. O'Leary

Biological Sciences

Resveratrol is a polyphenol antioxidant found in red wine known to have anti-aging, anti-cancer, and anti-obesity effects in both humans and animals. Resveratrol's primary biological action is as an antioxidant, causing a reduction in reactive oxygen species (ROS). ROS are produced as byproducts of normal metabolic functions, but become abnormally elevated in individuals who consume a high fat diet (HFD) or who have metabolic conditions like Type II Diabetes Mellitus (T2DM); in these circumstances, abnormal elevation in ROS leads to cellular damage and cell death. Another common effect of a diet high in fat and calories is cognitive impairment ...


The Differential Splicing Of The Socs2 5’Utr, A Gene Involved In Successful Central Nervous System Axon Regeneration In Xenopus Laevis, Megan Gura May 2015

The Differential Splicing Of The Socs2 5’Utr, A Gene Involved In Successful Central Nervous System Axon Regeneration In Xenopus Laevis, Megan Gura

Biological Sciences

The amphibian Xenopus laevis has the ability to regenerate axons of its optic nerve even after metamorphosis. From previous studies done in our lab, we found that Suppressor of Cytokine Signaling 2 (SOCS2) could be involved in the complex pathway of genes regulating nervous system development and regeneration. 3’ and 5’ Rapid Amplification of cDNA Ends (RACE) revealed that the 5’ untranslated region (UTR) of SOCS2 contains two splice forms. One splice form contained a previously unidentified 68 base pair exon, which will be referred to as ‘Exon 2’, which we hypothesized is involved in post-transcriptional regulation of SOCS2. I ...


The Rck/P54 Prion-Related Domain And Its Influence On The Localization And Function Of Rck/P54 During Hcv Infection, Lindsey Oltz May 2015

The Rck/P54 Prion-Related Domain And Its Influence On The Localization And Function Of Rck/P54 During Hcv Infection, Lindsey Oltz

Biological Sciences

Currently over 180 million people are infected with Hepatitis C virus (HCV) worldwide. HCV infection is a major cause of hepatocellular carcinoma (HCC), liver cirrhosis, and chronic hepatitis. The available antiviral treatment of interferon and ribavirin has limited success, is costly and toxic. Furthermore, there is no vaccine. An increased understanding of how HCV exploits the many cellular pathways and host factors during infection will provide information necessary for the development of novel anti-HCV therapies, which target host proteins rather than the rapidly evolving viral proteins. During infection, HCV interacts with and manipulates many host mRNA pathways. The Pager lab ...


A Preliminary Report On Escherichia Coli Strain Diversity In Cows, Alison T. Stivers Mar 2015

A Preliminary Report On Escherichia Coli Strain Diversity In Cows, Alison T. Stivers

Biological Sciences

Pyroprinting is a strain typing method that relies on the simultaneous pyrosequencing of the multi-copy rRNA intergenic transcribed spacer regions of E. coli (Black, et al., 2014). These pyroprints can be used to identify the source of E. coli in the environment. Currently, Cal Poly’s Center for Applications in Biotechnology (CAB) is augmenting the existing E. coli pyroprint library. By pyroprinting the intestinal E. coli of cows, we can quantify the strain diversity present, evaluate persistence, and determine the minimum sample size required for a complete overview of the cow intestinal E. coli population. These pyroprints can then be ...