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Theses/Dissertations

2013

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Full-Text Articles in Life Sciences

Tissue-Dependent T Cell Apoptosis And Transcriptional Regulation Of Memory Cd8+T Cell Differentiation During Viral Infections: A Dissertation, Varun N. Kapoor Dec 2013

Tissue-Dependent T Cell Apoptosis And Transcriptional Regulation Of Memory Cd8+T Cell Differentiation During Viral Infections: A Dissertation, Varun N. Kapoor

GSBS Dissertations and Theses

Activation and proliferation of antigen-specific T cells is the hallmark of an anti-viral immune response. Effector T cells generated during an immune response are heterogeneous in regards to their ability to populate the memory pool once the immune response has resolved. Initial T cell activation takes place in the lymphoid organs, after which T cells migrate into the non-lymphoid tissues. The presence of memory T cells at non-lymphoid tissue sites has been shown to be critical for protection against secondary virus challenge. Our lab has previously demonstrated that during and after the resolution of the immune response to Lymphocytic choriomeningitis ...


Understanding Regulation Of The Cytoskeleton During Cell Cycle Transitions Through Examination Of Crosstalk Between Homologous Fission Yeast Pathways, Septation Initiation Network And Morphogenesis Orb6 Network: A Dissertation, Sneha Gupta Dec 2013

Understanding Regulation Of The Cytoskeleton During Cell Cycle Transitions Through Examination Of Crosstalk Between Homologous Fission Yeast Pathways, Septation Initiation Network And Morphogenesis Orb6 Network: A Dissertation, Sneha Gupta

GSBS Dissertations and Theses

The fission yeast Schizosaccharomyces pombe has become a powerful model system for studying cytokinesis, a process of cytoplasmic division by which one cell divides into two identical daughter cells. Like mammalian cells, S. pombe divides through the use of an actomyosin contractile ring, which is composed of a set of highly conserved cytoskeletal proteins. Cytokinesis in S. pombe is primarily regulated by the SIN pathway, which is activated in late mitosis and is required for actomyosin contractile ring and septum assembly, and also plays a role in spindle checkpoint inactivation, and telophase nuclear positioning. The various functions of the SIN ...


Elucidating The Factors That Modulate The Distribution Of Avian Haemosporida Parasites Across A Community Of Hosts, Matthew Christopher Ikaika Medeiros Dec 2013

Elucidating The Factors That Modulate The Distribution Of Avian Haemosporida Parasites Across A Community Of Hosts, Matthew Christopher Ikaika Medeiros

Dissertations

Parasites are heterogeneously distributed across host species, host populations, and host individuals within populations. A primary aim of infectious disease ecology seeks to uncover the factors that drive this heterogeneity. At a fundamental level, host infection is determined by exposure and susceptibility to a pathogen. My dissertation explores how evolutionary and ecological forces associated with these fundamental determinates of infection shape variation in parasite host breadth and host infection status. Here, I focus on a community of vector-borne avian Haemosporida parasites among suburban birds of Chicago, IL. These parasites exhibit strong variation in their distribution among available hosts, and provide ...


Exploring The Local And Regional Effects Of Plant Diversity On Plant Herbivore Interactions, Diego Salazar Dec 2013

Exploring The Local And Regional Effects Of Plant Diversity On Plant Herbivore Interactions, Diego Salazar

Dissertations

The study of biological diversity and its effects on ecosystem functioning and species interactions has always been a fundamental part of biology. The accelerating loss of species in conjunction with an increasing change in the natural environment has underlined the importance of the role that biodiversity has on the evolutionary and ecological dynamics of natural systems. In this dissertation I explore the effect of local and regional patterns of plant diversity in plant-herbivore interactions. Furthermore, this work goes beyond the classical concepts of taxonomical diversity and investigates the role of phylogenetic and chemical diversity on plant-herbivore interactions. In chapter one ...


The Response Of Naive Channel Catfish To Chemical Cues Associated With Predation, Marinda Coulter Dec 2013

The Response Of Naive Channel Catfish To Chemical Cues Associated With Predation, Marinda Coulter

Theses and Dissertations

The chemosensory abilities of fishes, are important in order to understand how prey can perceive and avoid predators. Predator-naïve Channel catfish were exposed to four extracts over eight sessions (naïve bass water (NBW), bass that were fed catfish water (BFCW), catfish skin extract (CS), and naïve bass water paired with catfish skin extract (CO)) to determine whether they have an innate predator response to potential chemical cues indicating possible predation risk. Movement was quantified as grid squares crossed, directional changes, and tail beats. Response increased during the first minute following stimulus injection and decreased during the second minute. Channel catfish ...


Regulation Of Eukaryotic Mcm2-7 Activity, Lance F. Dasilva Dec 2013

Regulation Of Eukaryotic Mcm2-7 Activity, Lance F. Dasilva

Electronic Thesis and Dissertation Repository

The transfer of genetic material from one cell generation to the next requires precise genome duplication. Aberrant DNA replication can lead to genomic instability and contribute to diseases arising from an unregulated cell cycle, such as cancer. Replicative DNA polymerases require a single-stranded (ssDNA) template from which to produce newly synthesized DNA. In eukaryotes, ssDNA is generated by the heterohexameric minichromosome maintenance 2 through 7 (Mcm2-7) replicative helicase that unwinds duplex DNA. Strict temporal separation of helicase loading and activation at multiple replication origins ensures once per cell cycle replication. The processes involved in activating Mcm2-7 to unwind DNA during ...


Who's Your Daddy? A Study Of Extra-Pair Copulation And Mating Behaviors Of Protonotaria Citrea, Morton Catherine Heidrich Dec 2013

Who's Your Daddy? A Study Of Extra-Pair Copulation And Mating Behaviors Of Protonotaria Citrea, Morton Catherine Heidrich

Theses and Dissertations

Mating behavior has a profound impact on reproductive success and the resulting genetic structure of offspring. Extra-pair copulation is a widely observed behavior within avian species. This study explored the genetic effects of mating behaviors of Prothonotary warblers, Protonotaria citrea (Parulidae), using co-dominant microsatellite markers. Prothonotary warblers are migratory songbirds that build nests in cavities, commonly found in wetland habitats. A set of artificial nest boxes were initiated by Dr Robert Reilly in 2002 in Dutch Gap, Chesterfield County Virginia, USA, a tidal tributary off the James River. From this population, 28 nest boxes were surveyed yielding 47 adults and ...


The Acute Effects Of Whole-Body Vibration Training On Passive And Dynamic Flexibility In Gymnasts, Caisa Nicole Brooks Dec 2013

The Acute Effects Of Whole-Body Vibration Training On Passive And Dynamic Flexibility In Gymnasts, Caisa Nicole Brooks

Theses and Dissertations

Gymnasts must attain extreme ranges of flexibility to execute performance requirements, thus effective stretching proves vital to advancement in the sport. This study examined the acute effects of whole-body vibration (WBV) on passive and dynamic flexibility in young, female gymnasts. Participants (n = 27, Junior Olympic levels 5-10) served as their own control. Measurements of passive and dynamic flexibility were obtained using the TOPS forward split testing method to examine passive flexibility and dynamic flexibility was measured via split jumps that were analyzed with video and Dartfish software. According to randomized order, all participants completed a stretching protocol either with the ...


Obm Thesis Project: Composing An Undergraduate Thesis Manual, William Strong Dec 2013

Obm Thesis Project: Composing An Undergraduate Thesis Manual, William Strong

Honors Theses

The Behavioral Research Supervisory System (BRSS) is a class designed to assist undergraduate and master students in completing a thesis project in a timely manner. Prior to beginning this project, undergraduate students were not utilizing the thesis manual for the class effectively and master students did not have an effective tool to grade the quality of their undergraduate thesis students’ work. Therefore, the ultimate goal of this thesis project is to create a better manual for undergraduate students to reference while working on their thesis projects and to provide a systematic grading system for their graduate student supervisors. A pre-manual ...


Pharmacokinetics Of Dexamethasone Delivered Via Iontophoresis, Justin Holbrook Rigby Dec 2013

Pharmacokinetics Of Dexamethasone Delivered Via Iontophoresis, Justin Holbrook Rigby

Theses and Dissertations

Study Design: Controlled laboratory study. Objectives: To determine the time course of dexamethasone sodium phosphate (Dex-P) iontophoresis delivery to underlying tissues using microdialysis. Background: The efficacy of iontophoresis at delivering Dex-P through the skin is unknown in humans because of the lack of minimally invasive measurement techniques. Methods: Sixty-four healthy male participants (age = 24.4 ± 3.3 yrs, height = 71.8 ± 2.5 in, weight = 181.8 ± 26.1 lbs) were randomly assigned into one of six groups: 1) 1 mA current, 1 mm probes depth ; 2) 1 mA current, 4 mm probes depth; 3) 2 mA current, 1 mm ...


Prenatal Programming Of Hepatic Glucose And Cholesterol Regulation In Male Rat Offspring By Chronic Intermittent Hypoxia, Waseem Iqbal Dec 2013

Prenatal Programming Of Hepatic Glucose And Cholesterol Regulation In Male Rat Offspring By Chronic Intermittent Hypoxia, Waseem Iqbal

Electronic Thesis and Dissertation Repository

Obstructive sleep apnea (OSA) is a chronic disorder involving repetitive interruptions in breathing during sleep. Sufferers of OSA are exposed to chronic intermittent hypoxia (CIH), characterized by cyclical reductions in oxygen availability. A number of studies have established a link between OSA and various cardiovascular and metabolic comorbidities in adulthood, including hypertension, obesity, and type II diabetes. While the consequences of OSA in adults have been well described, the cross-generational impact of this condition and potential effects on fetal development are not known. Epidemiological and animal studies have demonstrated that physiological insults during pregnancy lead to diminished growth of offspring ...


Attributing Meaning To Online Social Network Analysis For Tailored Socio-Behavioral Support Systems, Sahiti Myneni Dec 2013

Attributing Meaning To Online Social Network Analysis For Tailored Socio-Behavioral Support Systems, Sahiti Myneni

UT SBMI Dissertations (Open Access)

Ubiquitous online social networks provide us with a unique opportunity to deliver scalable interventions for the support of lifestyle modifications in order to change behaviors that predispose toward cancer and other diseases. At the same time these networks act as rich data sources to inform our understanding of end-user needs. Traditionally, social network analysis is based on communication frequency among members. In this work, I introduce communication content as a complementary frame for studying these networks.

QuitNet, an online social network developed to provide smoking cessation support is considered for analysis. Qualitative coding, automated content analysis, and network analysis were ...


Tundra Swan (Cygnus Columbianus Columbianus) Habitat Selection During The Nonbreeding Period, Katelyn H. A. Weaver Dec 2013

Tundra Swan (Cygnus Columbianus Columbianus) Habitat Selection During The Nonbreeding Period, Katelyn H. A. Weaver

Electronic Thesis and Dissertation Repository

Changes in availability and foraging profitability of agricultural and aquatic habitats have the potential to greatly influence population dynamics of waterfowl. Therefore, the purpose of my research was to understand habitat selection by Tundra Swans during the nonbreeding period and to explore the scale-dependency of these relationships. Habitat selection was influenced by seasonal changes in nutritional requirements and food availability; Tundra Swans selected open water and agriculture in winter, wetlands were weakly selected during migration when open water was strongly selected (especially during autumn), and there was a 2-fold increase in use of agriculture from autumn to spring. It appears ...


Contribution Of Trpm2 To Memory Loss In An Alzheimer's Mouse Model, Megan M. Chen Dec 2013

Contribution Of Trpm2 To Memory Loss In An Alzheimer's Mouse Model, Megan M. Chen

Electronic Thesis and Dissertation Repository

Alzheimer’s disease (AD) is a neurodegenerative disease characterized by the progressive deterioration of memory and other intellectual abilities. Accumulation of amyloid-β (Aβ) peptide, the major contributor to the senile plaques central to AD, is thought to mediate neurotoxicity by inducing oxidative stress and calcium dysregulation. Transient Receptor Potential Melastatin type 2 (TRPM2) is a calcium permeable, non-selective cation channel activated under oxidative stress and ultimately induces cell death. The APPSWE/PSEN1ΔE9 double transgenic mouse model carries the human APPswe (Swedish mutations K594N/M595L) and PS1 mutations with a deletion in exon 9 (PS1-dE9), and is one of the most ...


Metabolic Remodeling And Mitochondrial Dysfunction In Maladaptive Right Ventricular Hypertrophy Secondary To Pulmonary Arterial Hypertension, Jose Gomez-Arroyo Dec 2013

Metabolic Remodeling And Mitochondrial Dysfunction In Maladaptive Right Ventricular Hypertrophy Secondary To Pulmonary Arterial Hypertension, Jose Gomez-Arroyo

Theses and Dissertations

Right ventricular dysfunction is the most frequent cause of death in patients with pulmonary arterial hypertension. Although abnormal energy substrate use has been implicated in the development of chronic left heart failure, data describing such metabolic remodeling in failing right ventricular tissue remain incomplete. In the present dissertation we sought to characterize metabolic gene expression changes and mitochondrial dysfunction in functional and dysfunctional RV hypertrophy. Two different rat models of RV hypertrophy were studied. The model of right ventricular failure (SU5416/hypoxia) exhibited a significantly decreased gene expression of peroxisome proliferator-activated receptor- coactivator-1α, peroxisome proliferator- activated receptor-α and estrogen-related receptor-α ...


Song As An Honest Indicator Of Developmental Stress In Song Sparrows, Kimberly L. Schmidt Dec 2013

Song As An Honest Indicator Of Developmental Stress In Song Sparrows, Kimberly L. Schmidt

Electronic Thesis and Dissertation Repository

The Developmental Stress Hypothesis proposes that the honesty of birdsong is maintained by costs incurred during development, such that song in adulthood reflects exposure to early-life stressors. The purpose of this thesis was to provide a rigorous test of the Developmental Stress Hypothesis in song sparrows (Melospiza melodia). My three objectives were to determine the long-term effects of early-life stress on: 1) physiological traits (Chapters 2, 3, and 4); 2) male song production (Chapter 5); and 3) the response of females to song (Chapter 6). Nestlings were hand-reared in captivity under one of three treatment conditions: control, food restriction, or ...


Formation And Analysis Of Zinc Oxide Nanoparticles And Zinc Oxide Hexagonal Prisms And Optical Analysis Of Cadmium Selenide Nanoparticles, Jared M. Hancock Dec 2013

Formation And Analysis Of Zinc Oxide Nanoparticles And Zinc Oxide Hexagonal Prisms And Optical Analysis Of Cadmium Selenide Nanoparticles, Jared M. Hancock

Theses and Dissertations

In this dissertation, methods to synthesize ZnO are reported. First, zinc oxide nanoparticles were synthesized with small amounts of transition metal ions to create materials called dilute magnetic semiconductors (DMS). We employed a low temperature sol-gel method that produces ZnO nanoparticles of reproducible size and incorporates cobalt, nickel, and manganese ions into the nanoparticles. Conditions were controlled such that a range of amounts of Co, Ni, and Mn were incorporated. The incorporation was tracked by color changes in the white ZnO powder to blue for Co, green for Ni and yellow for Mn. XRD measurements showed the nanoparticles were on ...


Regulation Of Satiety Quiescence: Cyclic Gmp, Tgf Beta, And The Asi Neuron, Thomas Gallagher Dec 2013

Regulation Of Satiety Quiescence: Cyclic Gmp, Tgf Beta, And The Asi Neuron, Thomas Gallagher

Theses and Dissertations

The worm Caenorhabditis elegans is a well-studied model organism in numerous aspects of its biology. This small free living nematode has less than 1,000 cells, but shows clear conservation in both signaling and behavior to mammals in aspects of appetite control. This is of importance to humans, where failure of appetite control is a major factor in the unprecedented obesity epidemic that we see today. In general, worm behavior reflects its internal nutritional state and the availability and quality of food. Specifically, worms show a behavioral state that mimics aspects of the mammalian behavioral satiety sequence, which has been ...


Effects Of Biodiesel Concentration On Microbial Deterioration Of Polyethylene In A Simulated Fuel Storage Tank, Juan Manuel Restrepo-Florez Dec 2013

Effects Of Biodiesel Concentration On Microbial Deterioration Of Polyethylene In A Simulated Fuel Storage Tank, Juan Manuel Restrepo-Florez

Electronic Thesis and Dissertation Repository

In this study a simulated fuel storage tank was used to investigate the effect of biodiesel concentration on biodegradation of polyethylene. This research is relevant in the field of fuel storage. The simulated storage system consisted of a number of identical conical flasks. Each flask was comprised of two layers, an upper one consisting of a fuel blend of diesel with biodiesel in concentrations ranging from 0 to 100% of biodiesel and the bottom layer containing an aqueous mineral media inoculated with a community obtained from a real fuel storage facility. Polyethylene slabs cut to a specific size were immersed ...


Bee And Wasp Species Diversity At A Restored Wetland In Seneca Falls, New York, Sabrina Green Dec 2013

Bee And Wasp Species Diversity At A Restored Wetland In Seneca Falls, New York, Sabrina Green

Honors Theses

When monitoring the health and any changes in an ecosystem, having a baseline of species present in the area is important to be able to monitor changes over time. I was interested in the bee and wasp species richness at the Seneca Meadows Wetland Preserve in Seneca Falls, New York as it is a five year old restored wetland with a diverse range of habitat types. I hypothesized that the esker habitat, at the crux between wetland and upland habitat would have the greatest species richness. Using cup traps and sweep netting, I obtained and identified specimens from five major ...


Instructional Preferences In Aquatics For Children With Visual Impairments And Their Instructors, Fabiana Cieslak Dec 2013

Instructional Preferences In Aquatics For Children With Visual Impairments And Their Instructors, Fabiana Cieslak

Kinesiology, Sport Studies, and Physical Education Master’s Theses

The aim of this study was to determine which instructional strategies athletes with visual impairments and their coaches would prefer during swimming classes. Thirteen athletes with visual impairments and fourteen coaches participated in interviews to reveal their preferences. A thematic analysis was utilized to ensure the analysis was undertaken in a theoretically and methodologically sound manner. Three key themes emerged, each a compilation of a set of subthemes. The first theme, physical guidance, included a quicker learning process and passive and active learning. The second theme, tactile modeling, was comprised of barriers and better instruction. The final theme that emerged ...


Snail Induction Of Nuclear Erk 1/2 Promotes Epithelial-Mesenchymal Transition And Chemotherapy Resistance In Breast Cancer Cells, Bethany Nicole Smith Dec 2013

Snail Induction Of Nuclear Erk 1/2 Promotes Epithelial-Mesenchymal Transition And Chemotherapy Resistance In Breast Cancer Cells, Bethany Nicole Smith

ETD Collection for AUC Robert W. Woodruff Library

Snail is high in several cancers, correlates with poor clinical prognosis, and associated with increased tumor progression via epithelial-to-mesenchymal transition (EMT). EMT is a latent embryonic program that alters epithelial cells to appear more mesenchymal, regulates embryonic development and wound healing. Snail binds to enhancer box sequences on its target genes like E-cadherin, maspin, and estrogen receptor-alpha (ER-a) to increase EMT. MAP kinase (MAPK/ERK1/2) protein signaling regulates the effects of EMT during tumor progression by regulating cell proliferation, growth, migration, adhesion, invasion, and survival. Recent reports suggest that ERK1 and ERK2 isoforms may function differently although they share ...


Whey Proteins Cross-Linked By Transglutaminase Or Glycated With Maltodextrin: Physicochemical Bases Of The Improved Heat Stability, Wan Wang Dec 2013

Whey Proteins Cross-Linked By Transglutaminase Or Glycated With Maltodextrin: Physicochemical Bases Of The Improved Heat Stability, Wan Wang

Doctoral Dissertations

Whey protein, as a byproduct in cheese manufacturing, is an ideal source for producing ready-to-drink protein beverages for different market demands, attributed to its high content of essential amino acids and versatile functionalities, bland flavor and good digestibility. Whey protein is a mixture of proteins, which can be categorized into whey protein concentrate (WPC) with a protein content of 50~80% and whey protein isolate (WPI) with a protein content higher than 90%, depending on different separation techniques. Thermal processing is required to ensure microbiological safety and quality of dairy products, leading to denaturation of whey proteins, especially at pH ...


Influence Of Genetic Variation In The Biotic Environment On Phenotypic Variation In A Plant-Feeding Insect, Darren Rebar Dec 2013

Influence Of Genetic Variation In The Biotic Environment On Phenotypic Variation In A Plant-Feeding Insect, Darren Rebar

Theses and Dissertations

While many species spend much of their lives in close association with other organisms, only recently have biologists started to explore the implications of the biotic nature of environments for their role as causes of variation in phenotypes. This means that the genotypes of individuals that constitute the biotic environment may influence the phenotypes of individuals that live in that environment. These are called indirect genetic effects (IGEs) when they occur between conspecifics, and interspecific indirect genetic effects (IIGEs) when they occur between heterospecifics. However, the impact of genetic variation in biotic environments remains largely unknown. I used a member ...


The Role Of The Amygdala, Retrosplenial Cortex, And Medial Prefrontal Cortex In Trace Fear Extinction And Reconsolidation, Janine Lynn Kwapis Dec 2013

The Role Of The Amygdala, Retrosplenial Cortex, And Medial Prefrontal Cortex In Trace Fear Extinction And Reconsolidation, Janine Lynn Kwapis

Theses and Dissertations

A wealth of research has outlined the neural circuits responsible for the consolidation, reconsolidation, and extinction of standard "delay" fear conditioning, in which awareness is not required for learning. Far less is understood about the neural circuit supporting more complex, explicit associations. "Trace" fear conditioning is considered to be a rodent model of explicit fear because it relies on the cortex and hippocampus and requires explicit contingency awareness in humans for successful acquisition. In the current set of studies, we aimed to better characterize the neural circuit supporting the consolidation, reconsolidation, and extinction of trace fear in order to better ...


Phylogeographic Inference Of Insular Mule Deer (Odocoileus Hemionus) Divergence In North America's Desert Southwest, Ona Alminas Dec 2013

Phylogeographic Inference Of Insular Mule Deer (Odocoileus Hemionus) Divergence In North America's Desert Southwest, Ona Alminas

Theses and Dissertations

Though mule deer (Odocoileus hemionus) persist in robust populations throughout most of their North American distribution, nearly 60% of their historic range in México has declined due to habitat loss and unregulated hunting. Two of the six subspecies inhabiting México's deserts and Baja California peninsula are of conservation concern, occurring on land bridge islands in the Pacific Ocean (O. h. cerrosensis on Cedros Island: threatened) and in the Sea of Cortés (O. h. sheldoni on Tiburón Island: endangered). Focusing on the desert southwest (n=449 deer), we obtained 1,611 bp of mtDNA sequence (control region: 583 bp; cytochrome ...


Transcending Microbial Source Tracking Techniques Across Geographic Borders: An Examination Of Human And Animal Microbiomes And The Integration Of Molecular Approaches In Pathogen Surveillance In Brazil And The United States, Amber Mae Koskey Dec 2013

Transcending Microbial Source Tracking Techniques Across Geographic Borders: An Examination Of Human And Animal Microbiomes And The Integration Of Molecular Approaches In Pathogen Surveillance In Brazil And The United States, Amber Mae Koskey

Theses and Dissertations

Waterborne illnesses, attributed to the ingestion or contact with contaminated water, present a significant global health concern. Surface water sources can be impacted by wide array of pollution inputs, but fecal pollution generates the most significant and acute threat to human health. Therefore, the detection of fecal bacteria in surface water sources remains an important public health objective. Current surface water monitoring employs the use of fecal indicator bacteria (FIB) including E. coli and enterococci as proxies for pathogenic organisms carried in fecal pollution. These traditional indicators, detected by culture-based microbiological methods, do not discriminate fecal sources from another. New ...


Neural Responses During Trace Conditioning With Face And Non-Face Stimuli Recorded With Magnetoencephalography, Nicholas Lee Balderston Dec 2013

Neural Responses During Trace Conditioning With Face And Non-Face Stimuli Recorded With Magnetoencephalography, Nicholas Lee Balderston

Theses and Dissertations

During fear conditioning a subject is presented with an initially innocuous stimulus like an image (conditioned stimulus; CS) that predicts an aversive outcome like a mild electric shock (unconditioned stimulus; UCS). Subjects rapidly learn that the CS predicts the UCS, and show autonomic fear responses (CRs) during the presentation of the CS. When the CS and the UCS coterminate, as is the case for delay conditioning, individuals can acquire CRs even if they are unable to predict the occurrence of the UCS. However when there is a temporal gap between the CS and the UCS, CR expression is typically dependent ...


Evaluation Of Vdr-Coactivator Inhibitors Using Biochemical And Cell-Based Assays, Athena Marie Baranowski Dec 2013

Evaluation Of Vdr-Coactivator Inhibitors Using Biochemical And Cell-Based Assays, Athena Marie Baranowski

Theses and Dissertations

ABSTRACT

EVALUATION OF VDR–COACTIVATOR INHIBITORS USING BIOCHEMICAL AND CELL–BASED ASSAYS

by

Athena Baranowski

The University of Wisconsin–Milwaukee, 2013

Under the Supervision of Dr. Alexander Arnold

The vitamin D receptor (VDR) is a ligand–dependent transcription factor, which belongs to the nuclear receptor superfamily. VDR–mediated gene regulation is governed by coregulators (coactivators and corepressors). VDR coregulator binding inhibitors (CBIs), which were discovered using high throughput screening (HTS), were evaluated using cell–based assays and biochemical assays to determine their ability to inhibit the interaction between VDR and steroid receptor coactivator–2 (SRC–2). Determining their ability to ...


Structure And Function Of Proteins Investigated By Crystallographic And Spectroscopic Time-Resolved Methods, Namrta Purwar Dec 2013

Structure And Function Of Proteins Investigated By Crystallographic And Spectroscopic Time-Resolved Methods, Namrta Purwar

Theses and Dissertations

Biomolecules play an essential role in performing the necessary functions for life. The goal of this thesis is to contribute to an understanding of how biological systems work on the molecular level. We used two biological systems, beef liver catalase (BLC) and photoactive yellow protein (PYP). BLC is a metalloprotein that protects living cells from the harmful effects of reactive oxygen species by converting H2O2 into water and oxygen. By binding nitric oxide (NO) to the catalase, a complex was generated that mimics the Cat-H2O2 adduct, a crucial intermediate in the reaction promoted by the catalase. The Cat-NO complex is ...