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Theses/Dissertations

2013

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Full-Text Articles in Life Sciences

The Development Of An In Vivo Microdialysis Collection Method Of Cytokines From Brain Tissue, Anthony W. Herbaugh Dec 2013

The Development Of An In Vivo Microdialysis Collection Method Of Cytokines From Brain Tissue, Anthony W. Herbaugh

Theses and Dissertations

In this thesis, different methods to improve the microdialysis collection procedure for cytokines from brain tissue are presented. The first method was based on stopped flow and results indicating that no significant difference in relative recovery between stopped flow and continuous flow are shown. The second method is an antibody bead-based enhancement method. With the antibody bead-based method, a 3.5 fold increase in the collected concentrations of Chemokine (C-C motif) Ligand 2 (CCL2) were observed. However, there was no significant increase in the in vivo collection efficiency of Interleukin-6 (IL-6) using the antibody enhancement. Finally the development of an ...


Greater Sage-Grouse (Centrocercus Urophasianus) Habitat Selection In Northwestern Wyoming And Stable Isotope Analysis Of Fecal Material, Andrea Renee Green Dec 2013

Greater Sage-Grouse (Centrocercus Urophasianus) Habitat Selection In Northwestern Wyoming And Stable Isotope Analysis Of Fecal Material, Andrea Renee Green

Theses and Dissertations

Dramatic range-wide declines in Greater Sage-Grouse populations have prompted efforts to determine habitat characteristics that are selected by sage-grouse for foraging, nesting and brood-rearing areas in an effort to conserve this species. Managers at Heart Mountain and Y U Bench in northwestern Wyoming expressed the need to quantify various habitat characteristics and to determine key use areas at both study sites. Data were collected on a variety of habitat variables in spots selected by grouse for foraging, nesting, and brood-rearing activities. These variables were compared to the same variables measured at random points at both study sites. Significant differences existed ...


Age, Growth, Mortality, And The Effects Of Trawling On Mortality Of The Longspine Porgy, Stenotomus Caprinus, Cher Marie Newman Dec 2013

Age, Growth, Mortality, And The Effects Of Trawling On Mortality Of The Longspine Porgy, Stenotomus Caprinus, Cher Marie Newman

Master's Theses

Stenotomus caprinus (Longspine Porgy) is one of the most abundant bycatch species caught in trawl fisheries in the northern Gulf of Mexico (GOM). Biological data of length, weight, and abundance have been collected since 1972 on the Southeast Area Monitoring and Assessment Program (SEAMAP) surveys with a variety of GOM species, including Longspine Porgy, using trawl nets during groundfish surveys. In 2010-2011 five Longspine Porgy were obtained from Groundfish and Pelagic surveys at each station for further analysis. Age distribution was determined through von Bertalanffy length and weight-age models. The samples were separated into two regions of the GOM to ...


Assessing Respiration Rates And Nutrient Dynamics Of Aritifical Reef Biofilms And Bacterioplankton In The Mississippi Sound, Lynn Elizabeth Wilking Dec 2013

Assessing Respiration Rates And Nutrient Dynamics Of Aritifical Reef Biofilms And Bacterioplankton In The Mississippi Sound, Lynn Elizabeth Wilking

Master's Theses

Artificial reefs are primarily used to provide a suitable habitat for target fish populations, but the structures can also improve water quality and benefit non-target organisms. Laboratory incubation experiments were conducted in the presence of biofilm on rubble and in its absence to examine bacterial growth, community respiration, and nutrient dynamics at four artificial reef habitats in the Mississippi Sound. Biofilm samples were also collected from settlement plates deployed at each site and were analyzed for 813C and 81sN stable isotope content. Respiration rates were always higher in the presence of biofilm but bacterial abundance often declined over time, and ...


Animal Welfare Around The World: Examining The Role The Economy Has On Animal Welfare, Olivia J. Thornton Dec 2013

Animal Welfare Around The World: Examining The Role The Economy Has On Animal Welfare, Olivia J. Thornton

Chancellor’s Honors Program Projects

No abstract provided.


The Health Monitoring Of Naïve, Immunocompromised Sows In A Seasoned Facility, Sara Nash Dec 2013

The Health Monitoring Of Naïve, Immunocompromised Sows In A Seasoned Facility, Sara Nash

Chancellor’s Honors Program Projects

No abstract provided.


Connecting The Dots: Understanding Migration In The Context Of Other Periods Of The Annual Cycle, Kristina Lee Paxton Dec 2013

Connecting The Dots: Understanding Migration In The Context Of Other Periods Of The Annual Cycle, Kristina Lee Paxton

Dissertations

Migration is a fundamental characteristic of the life history of many organisms. Large-scale seasonal movements expose migratory species to an array of differing environmental conditions such that the ecology, behavior, and life history strategies of migratory species must balance selection pressures associated with each phase of the annual cycle. Therefore, scientists increasingly realize that to understand the ecology of a migratory species in any one phase of the annual cycle requires an understanding of how other phases may interact with and influence the period of interest. That said, following individuals throughout the year poses a serious challenge.

The focus of ...


Filter-Based Multiscale Entropy Analysis Of Complex Physiological Time Series, Liang Zhao Dec 2013

Filter-Based Multiscale Entropy Analysis Of Complex Physiological Time Series, Liang Zhao

Dissertations - ALL

The multiscale entropy (MSE) has been widely and successfully used in analyzing the complexity of physiologic time series. In this thesis, we re-interpret the averaging process in MSE as filtering a time series by a filter of a piecewise constant type. From this viewpoint, we introduce the {\it filter-based multiscale entropy} (FME) which filters a time series by filters to generate its multiple frequency components and then compute the {\it blockwise} entropy of the resulting components. By choosing filters adapted to the feature of a given time series, FME is able to better capture its multiscale information and to provide ...


Recombinant Expression, Purification, And Characterization Of An Hiv-1 Tat-Human Cyclin T1 Chimera, Collin Lesley Fischer Dec 2013

Recombinant Expression, Purification, And Characterization Of An Hiv-1 Tat-Human Cyclin T1 Chimera, Collin Lesley Fischer

Dissertations - ALL

Efficient transcription of the human immunodeficiency virus type 1 (HIV-1) requires the interaction of the viral protein Tat with the trans-activation response (TAR) stem-loop of the long-terminal repeat (LTR) portion of nascent viral RNA. The production of viable transcripts is enhanced dramatically by the interaction of HIV-1 Tat with the host protein human Cyclin T1. Interaction with hCycT1 remodels Tat protein contributing a single cysteine residue that is critical to the formation of the second of two zinc fingers (Zn2). Here we suggest that it is the presence of this critical cysteine residue and not the presence of arginine residues ...


Mutant Drought Phenotype Investigation : A Look Into Effects Of Protein Prenylation On Water Utilization In Conditions Of Varying Soil Saturation And Gene Knockout., Casey Rasmussen Dec 2013

Mutant Drought Phenotype Investigation : A Look Into Effects Of Protein Prenylation On Water Utilization In Conditions Of Varying Soil Saturation And Gene Knockout., Casey Rasmussen

College of Arts & Sciences Senior Honors Theses

Plants respond to environmental stress, such as drought, with increases in levels of Abscisic Acid (ABA). This stress response allows greater survival rates but results in the plants producing lower seed yield and biomass. Certain mutant strains of the Arabidopsis plant have been identified with mutations in coding regions involved in the formation of the α and β subunits of PFT and PGGT-I genes involved in protein prenylation. We explored the possibility of these mutant strains to better preserve seed yield and plant biomass under drought stressing conditions in comparison to the wild type (Columbia) strain, based on the mutants ...


Development Of Novel Subunit Vaccine Against H5n1 Influenza, Lu Zhang Dec 2013

Development Of Novel Subunit Vaccine Against H5n1 Influenza, Lu Zhang

Theses and Dissertations

Influenza is a common infectious disease resulting from a frequently mutated RNA virus. Vaccination is currently the most effective method to prevent people from seasonal or pandemic influenza. The production of traditional egg-based influenza vaccine is time-consuming and provides limited effect against new strains. Therefore, it is necessary to develop a rapid method to produce influenza vaccines. We proposed a novel influenza vaccine based on the E.coli expression system. Hemagglutinin (HA) is the major target surface protein of influenza virus for vaccine development. In this study, we sub-cloned the HAs encoding gene into an E. coli expression vector; the ...


The Role Of Histone H3 And H4 In Centromere Function And Genome Integrity, Payel Chaudhuri Dec 2013

The Role Of Histone H3 And H4 In Centromere Function And Genome Integrity, Payel Chaudhuri

Theses and Dissertations

Histone H2A plays an important role in chromosomal segregation among parent and daughter cells during mitosis. While it is established that this histone is important in maintaining chromosome number in cell, further work is carried out to explore the role of other histones like H3 and H4 for similar effects. A systematic study is initiated by screening a library based on mutation of different amino acid residues in these histones. This detailed screening identified specific regions within H3 and H4, which are critically important for centromeric function. These histones residing near the DNA entry/exit region of nucleosome effects the ...


In Vitro Analysis Of Short Chain Fatty Acids And Human Fecal Microbiota Stimulated By Pectin Sources, Byungjick Min Dec 2013

In Vitro Analysis Of Short Chain Fatty Acids And Human Fecal Microbiota Stimulated By Pectin Sources, Byungjick Min

Theses and Dissertations

As interest increases for colonic health, various studies have been conducted to investigate functional ingredients for host health and prevention of chronic disease in humans such as colon cancer and Type 2 diabetes. In particular dietary fiber has been reported to have beneficial effects on colonic health. More recently, dietary fiber has been categorized based on its characteristics and numerous studies have been conducted to determine correlations with chronic diseases. Pectin is a naturally occurring biopolymer that has been used in various applications for purposes of pharmaceutical and biotechnology. Pectin is a soluble dietary fiber and can be entirely fermented ...


Evaluation Of Shake Weight Protocol In Senior Populations, Isaac F. Cook Dec 2013

Evaluation Of Shake Weight Protocol In Senior Populations, Isaac F. Cook

Theses and Dissertations

The Shake Weight® (SW®) is designed to improve muscular fitness in a quick and inexpensive way. This study aimed to determine if the SW® was an effective tool at improving muscular fitness, body composition, and bone mineral density (BMD) in post-menopausal women. Participants were 17 healthy, post-menopausal women from aMidwestern University and divided into two training (SW® and HIT) interventions that lasted 10 weeks. HIT participants performed three sets of 8 repetitions at 80% of their estimated 1RM for the chest press, leg press, lat pulldown, and seated row. SW® participants performed the exercises prescribed by the SW® manufacturer. Changes ...


Preservice Candidates' Rating Of Effectiveness In Agricultural Education Through Structured Communication With Cooperating Teachers, Christopher Lee Hunt Dec 2013

Preservice Candidates' Rating Of Effectiveness In Agricultural Education Through Structured Communication With Cooperating Teachers, Christopher Lee Hunt

Theses and Dissertations

The relationship between cooperating teacher and student has been found as one of the key elements that affect the overall teaching efficacy of student teachers and their decision to enter the teaching field after graduation (Edgar, 2007; Edgar, Roberts, & Murphy, 2011,2008; Kasperbauer et al., 2007a; Roberts, Greiman, Murphy, Ricketts, Harlin, & Briers 2009; Roberts, Harlin, & Briers, 2007, Roberts, Harlin, & Ricketts, 2006; Roberts Mowen, Edgar, Harlin & Briers, 2007, Stripling, Ricketts, Roberts & Harlin, 2008; Wolf, 2011; Wolf et al., 2010). Therefore, determining impacts towards teaching efficacy during the student teaching experience could play a vital role in future teachers' success. The purpose of this study was to assess teaching efficacy and the relationship between student teacher and cooperating teacher through ...


Biochemical And Biophysical Studies Of Novel Features Of Ras-Related Protein Interactions, Kyla Marie Morinini Morris Dec 2013

Biochemical And Biophysical Studies Of Novel Features Of Ras-Related Protein Interactions, Kyla Marie Morinini Morris

Theses and Dissertations

The Ras superfamily of G-proteins are of great research interest for structure-function relationships among proteins as they act as molecular switches in the regulation of various biochemical reactions in the cell. They are regulated by protein-protein interactions targeted to the highly flexible switch regions. Mutations in G-proteins or their effectors may cause alterations in structure and/or function that can lead to overactivity.

The Ras-related protein Cell division cycle 42 (Cdc42) is important in regulating cell-signaling processes. The T35A mutation in Cdc42 leads to a decrease in flexibility of the Switch I region responsible for effector binding. The kinetics of ...


Bacterially-Mediated Formation Of Rock Coatings In Kärkevagge, Swedish Lapland: A Mineralogical And Micro-Environmental Analog For Mars, Cassandra L. Marnocha Dec 2013

Bacterially-Mediated Formation Of Rock Coatings In Kärkevagge, Swedish Lapland: A Mineralogical And Micro-Environmental Analog For Mars, Cassandra L. Marnocha

Theses and Dissertations

The search for past or present life on Mars is, for now, limited to surface environments. An often neglected surface environment that could have served as an abode for life and could presently preserve evidence of that life is that of rock coatings. Rock coatings are mineral accretions on rock surfaces. On Earth, they are widespread and occur with considerable chemical diversity. There is growing evidence for a biotic role in their formation on Earth, particularly with respect to rock varnish. As a result, rock varnish has become a target of astrobiological interest on Mars, where varnish-like coatings have been ...


Sugar And Rice Trade Between The United States And Cuba Trade Potential And Welfare Analysis, Fabian Bloechl Dec 2013

Sugar And Rice Trade Between The United States And Cuba Trade Potential And Welfare Analysis, Fabian Bloechl

Theses and Dissertations

After the resignation of President Fidel Castro and forty-five years of the U.S.-trade embargo, the political environment between Cuba and the United States is expected to change. With the Trade Sanction Reform and Export Enhancement Act in 2000, trade sanctions were eased for a while. Future trade between both countries would increase the welfare in both countries. This Thesis has the approach to look at the impacts on the commodities of rice and sugar, and the trade sanctions between the United States and Cuba.

Cuba is a net importer of its food supply; therefore, agriculture and food trade ...


Long-Term Residue And Water Management Effects On Soil Respiration And Soil Aggregate Stability In A Wheat-Soybean, Double-Crop System In Eastern Arkansas, Sharon Faye Smith Dec 2013

Long-Term Residue And Water Management Effects On Soil Respiration And Soil Aggregate Stability In A Wheat-Soybean, Double-Crop System In Eastern Arkansas, Sharon Faye Smith

Theses and Dissertations

Sustainability in agriculture is paramount to assuring continued production from our most naturally fertile soils. Storing carbon (C) in soil as organic matter through sustainable agricultural management practices can both remove atmospheric C and improve soil quality. The objective of this study was to evaluate the long-term effects of water management (irrigation and dryland), residue management [burn and no-burn, conventional (CT) and no-tillage (NT)] and residue/fertility level (high and low) on soil respiration and aggregate stability in a wheat- (Triticum aestivum L.) soybean [Glycine max (L.) Merr.], double-crop system in a silt-loam soil (Aquic Fraglossudalf) in the Mississippi River ...


Quantitative Trait Loci Associated With Waterlogging Tolerance In A Soft Red Winter Wheat Mapping Population, Diana Carolina Ballesteros Benavides Dec 2013

Quantitative Trait Loci Associated With Waterlogging Tolerance In A Soft Red Winter Wheat Mapping Population, Diana Carolina Ballesteros Benavides

Theses and Dissertations

Waterlogging is caused when water stays superficially in the soil for an extended period of time, creating an anaerobic environment which decreases plant growth and grain yield at maturity. Despite the impact of waterlogging on wheat production both globally and in the southeastern U.S., very little is known about the genetic control of waterlogging tolerance in wheat. The objective of this study was to determine the amount of genetic variation for vegetative stage waterlogging tolerance present within a wheat recombinant inbred line (RIL) population and to identify quantitative trait loci (QTL) associated with tolerance and productivity. Experiments were carried ...


Post-Exercise And Post-Recovery Blood Lactate In Peripheral Arterial Disease, Rebecca Kate Roderick Dec 2013

Post-Exercise And Post-Recovery Blood Lactate In Peripheral Arterial Disease, Rebecca Kate Roderick

Theses and Dissertations

The purpose of this study was to observe how the presence of peripheral arterial disease affects the level of post-exercise blood lactate and post-recovery blood lactate during and following the six-minute walk test (6MWT). The 6MWT was administered to 49 participants (33 classified as Non-PAD and 16 classified as PAD) over the age of 50 with no uncontrolled cardiovascular or metabolic diseases or a cardiovascular event in the previous 6 months. Results demonstrated that no significant statistical difference exists between the presence of PAD and resting blood lactate (F = 0.86, p = .36), post-exercise blood lactate (F= 0.48, p ...


Risk Return Of Farmer-Elevator Contracts For Soybeans And Corn In Arkansas, Marei Undine Houpert Dec 2013

Risk Return Of Farmer-Elevator Contracts For Soybeans And Corn In Arkansas, Marei Undine Houpert

Theses and Dissertations

In Arkansas the contribution of Agriculture to the states GDP is comparatively high. To help farmer's return risk the grain industry developed several marketing tools to support farmers. Literature in this research field finds different results for different locations, commodities, marketing tools and marketing years. As Agriculture in Arkansas is important for its economy this study focuses on soybeans and corn produced in the fertile north-eastern area of Arkansas that uses Memphis Tennessee as a spot market palace. The examined marketing tools are pre-harvest futures hedges and forward contracts as well as post-harvest storage strategies and minimum price contracts ...


Congruent And Incongruent Effects Of Ethnic Music On Ethnic Menu Item Selection, Ryan David Muniz Dec 2013

Congruent And Incongruent Effects Of Ethnic Music On Ethnic Menu Item Selection, Ryan David Muniz

Theses and Dissertations

This study investigated what impact ethnic music had on ethnic menu item selection. College students were randomly divided into three groups and listened to one randomly assigned sound treatment in sensory booths. Three hundred and five participants completed the questionnaire with measures of expected price value and willingness to pay value. One of the groups listened to an Italian folk music, the second group listened to a Thai folk music, and the third group listened to a restaurant background noise in order to examine if the music will impact each participant menu items selection and perceived price values. The result ...


Sources Of Variability In Agronomic Weed Seed Predation: Time, Space, Habitat, And Hyperpredation, Sonja K. Birthisel Dec 2013

Sources Of Variability In Agronomic Weed Seed Predation: Time, Space, Habitat, And Hyperpredation, Sonja K. Birthisel

Electronic Theses and Dissertations

Weed seed predation is an ecosystem service that benefits farmers by decreasing seedbank inputs, thereby reducing weed pressure in subsequent growing seasons. Seed predation can be considerable, but is highly variable. Sources of variability may include time, space, habitat, and trophic interactions such as hyperpredation. Two experiments were conducted to measure the impacts of these sources of variability on weed seed predation rates in Maine mixed vegetable agroecosystems.

Chapter One of this thesis describes a series of landscape-level field experiments conducted to quantify the effects of time, space, and habitat on seed predation rates. Seed assays, with and without vertebrate ...


The Effect Of Wild Blueberry Consumption On The Inflammatory Response, Oxidative Stress And Dna Damage Associated With Exercise, Taylor K. Bloedon Dec 2013

The Effect Of Wild Blueberry Consumption On The Inflammatory Response, Oxidative Stress And Dna Damage Associated With Exercise, Taylor K. Bloedon

Electronic Theses and Dissertations

At all levels of intensity and duration, exercise is known to cause an increase in the generation of reactive oxygen species (ROS). When derivatives of oxygen occur and exist independently with one or more unpaired electrons they are known as “free radicals” (Halliwell and Gutteridge 2007). Since atoms possess electrons that are usually associated in pairs, free radicals have the potential to act negatively in the body. Reactive oxygen species refer to oxygen-centered radicals as well as nonradical but reactive derivatives of oxygen (Halliwell and Gutteridge 2007). When ROS are created in excess, resulting in the disruption of the pro-oxidant ...


Proximity-Labeling Of Near Neighbors Of Lamin A And Lamin A-Δ50 (Progerin)., Mohammad Sabri Dec 2013

Proximity-Labeling Of Near Neighbors Of Lamin A And Lamin A-Δ50 (Progerin)., Mohammad Sabri

Undergraduate Honors Theses

In an attempt to isolate and identify proteins that differentially interact with or locate near lamin A and progerin, we used a previously described method named BioID (proximity-dependent biotin identification). This method is based on fusion of a promiscuous E. coli biotin-protein ligase (BL) to a targeting protein (in this study, lamin A-GFP and progerin-GFP). The biotin ligase biotinylates amino residues in proteins that are near-neighbors of the fusion protein. To create the fusion proteins, BL was sub-cloned from a pcDNA3.1 MCS-BirA(R118G)-HA plasmid donated by Kyle Roux from University of South Dakota. The BL fragment was ligated ...


The Color And Consistency Of Steaks And Ground Beef Produced From Mature Bulls, Jace Joseph Hollenbeck Dec 2013

The Color And Consistency Of Steaks And Ground Beef Produced From Mature Bulls, Jace Joseph Hollenbeck

Theses and Dissertations

Two experiments were conducted to compare the color and cooked attributes of steaks and ground beef produced from mature bulls. Beef from mature bulls was used to test the effects of lactic acid (LA) enhancement solution pH (2.5, 3.0, or 3.5; B25, B30, B35, respectively) on fresh (5 d of display) and cooked color and tenderness of strip loin steaks (Exp. 1) and the effect of high pH trim on precooked ground beef patties (Exp. 2). In experiment 1, mature bull strip steaks were enhanced to 111% with a LA, sodium bicarbonate, and tap water solution, in ...


Population Pharmacokinetics And Exposure Toxicity Analysis Of Vatalanib In Patients With Myelodysplastic Syndromes, Xiaofeng Wang Dec 2013

Population Pharmacokinetics And Exposure Toxicity Analysis Of Vatalanib In Patients With Myelodysplastic Syndromes, Xiaofeng Wang

Theses and Dissertations

Vatalanib is a novel, potent, orally bioavailable angiogenesis inhibitor which can block all known vascular endothelial growth factor (VEGF) receptor tyrosine kinases. It has been shown to have potential in treating hematological malignancies, including myelodysplastic syndromes (MDS). Previous studies have revealed substantial pharmacokinetic variability that is further increased by auto-induction of its metabolism. And safety and tolerability are of great importance for such anti-angiogenic treatment. Therefore, the objectives of thesis was to characterize the pharmacokinetics and auto-induction process of vatalanib using population pharmacokinetic approaches, and to evaluate the relationship between systemic exposure to vatalanib and its toxicity in order to ...


Census And Mapping Of Chorro Creek Bog Thistle In Reservoir Canyon, San Luis Obispo, Ca, Tyler Michael Lutz Dec 2013

Census And Mapping Of Chorro Creek Bog Thistle In Reservoir Canyon, San Luis Obispo, Ca, Tyler Michael Lutz

Biological Sciences

Chorro Creek bog thistle (Cirsium fontinale var. obispoense) is a federally endangered variety of Fountain thistle endemic to western San Luis Obispo County. The U.S. Fish and Wildlife Service knows of nineteen populations, many with multiple colonies. A population was discovered in the Reservoir Canyon Natural Reserve in 2001, but has not been monitored or described since the time of its discovery. In fall of 2013, a census of the population was performed, the four colonies were mapped, and a floristic survey was conducted. A field experiment was initiated to determine if reducing the riparian canopy coverage can increase ...


The Imperative Of Conserving California's Foothill Oak Woodlands, Lauren Phillips Dec 2013

The Imperative Of Conserving California's Foothill Oak Woodlands, Lauren Phillips

Social Sciences

No abstract provided.