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Full-Text Articles in Life Sciences

Polyanhydride Particle Platform For Design Of Novel Influenza Vaccines, Lucas Mark Huntimer Jan 2013

Polyanhydride Particle Platform For Design Of Novel Influenza Vaccines, Lucas Mark Huntimer

Graduate Theses and Dissertations

Vaccines remain the most effective medical intervention to disease in which effective vaccines are available. Designing vaccines that elicit protective immunity while minimizing adverse events is difficult. Exacerbating the challenges of vaccine design is the increased emphasis on using pure preparation of antigens that alleviate safety concerns but also show decreased potency. Therefore the need for safe adjuvants to boost immunity of subunit immunizations is great. This work demonstrates the capability of a novel bio-erodible polyanhydride particle platform to enhance humoral and cellular immunity. Encapsulation of 25 µg of Ovalbumin (Ova) antigen in microparticles elicits humoral immune responses equivalent to ...


Immobilization And Characterization Of Physisorbed Antibody Films Using Pneumatic Spray As Deposition Technique, Jhon J. Figueroa Jan 2013

Immobilization And Characterization Of Physisorbed Antibody Films Using Pneumatic Spray As Deposition Technique, Jhon J. Figueroa

Graduate Theses and Dissertations

The immobilization of antibodies on silica surfaces has been a wide and common practice via cross-linking with the formation of covalent bonds between surface and antibody. The formation of antibody thin films on solid surfaces using pneumatic spray (PS) as the deposition technique and the analysis of the surface morphology of these films were investigated during this study. The pneumatic spray method was compared with the covalent bonding method Avidin-Biotin Bridge (ABB). The intensities and capture efficiency tests showed similar results for both techniques with a lower signal-to-noise ratio (SNR) for the PS deposited films. Specificity tests suggested that the ...