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Evolution And Functional Morphology Of The Cephalic Lobes In Batoids, Samantha Lynn Mulvany Dec 2013

Evolution And Functional Morphology Of The Cephalic Lobes In Batoids, Samantha Lynn Mulvany

Graduate Theses and Dissertations

Cephalic lobes are unique structures derived from the anterior pectoral fins, found in select myliobatid stingrays. Many benthic batoids utilize undulatory locomotion and use their pectoral fins for both locomotion and prey capture. Pelagic myliobatids that possess cephalic lobes utilize oscillatory locomotion, using their pectoral fins to locomote and their cephalic lobes for prey capture. Despite differences in habitat usage and locomotor modes, these batoids feed on very similar benthic organisms. The purpose of this study was to 1.) compare the morphology of the cephalic lobes and anterior pectoral fins in lobed and lobeless species, looking at skeletal elements, musculature ...


Skeletal Muscle Contraction Simulation: A Comparison In Modeling, Jonathan M. Ford Nov 2013

Skeletal Muscle Contraction Simulation: A Comparison In Modeling, Jonathan M. Ford

Graduate Theses and Dissertations

Computer generated three-dimensional (3-D) models are being used at increasing rates in the fields of entertainment, education, research, and engineering. One of the aspects of interest includes the behavior and function of the musculoskeletal system. One such tool used by engineers is the finite element method (FEM) to simulate the physics behind muscle mechanics. There are several ways to represent 3-D muscle geometry, namely a bulk, a central line of action and a spline model. The purpose of this study is to exmine how these three representations affect the overall outcome of muscle movement. This is examined in a series ...


Green Tea Polyphenols Prevent Parkinson's Disease: In Vitro And In Vivo Studies, Dan Chen Jan 2013

Green Tea Polyphenols Prevent Parkinson's Disease: In Vitro And In Vivo Studies, Dan Chen

Graduate Theses and Dissertations

ABSTRACT

Parkinson's disease (PD) is a progressive neurodegenerative disease, causing severe dopamine depletion in the striatum. Evidence shows that iron dyshomeostasis contributes to PD. Our long term goal is to improve the understanding of iron associated PD, thereby pave the way for nutritional prevention against PD in clinical trials. In this dissertation, the overarching hypotheses were that the neurotoxin 6-OHDA caused iron dyshomeostasis by regulating iron-related factors, increasing oxidative damage to neuron cells, while the iron chelator EGCG could counteract these adverse effects and prevent PD.

PD model cells are used to study the mechanism on disruption of iron ...


Effect Of Dietary Resistant Starch On Inhibition Of Preneoplasia In Azoxymethane-Induced Rodent Models, Bridget Nelson Jan 2013

Effect Of Dietary Resistant Starch On Inhibition Of Preneoplasia In Azoxymethane-Induced Rodent Models, Bridget Nelson

Graduate Theses and Dissertations

Diet choices can play a role in disease incidence, especially diseases associated with the digestive system, such as Irritable Bowel Syndrome, Crohn's disease, and colorectal cancer. Starch is a large component of the the human diet and the non-digestible portion, referred to as dietary fiber, has been implicated as a factor that decreases the risk for colorectal cancer. Modified or naturally occurring resistant starches share similar digestion resistant properties as dietary fiber and may produce similar protective effects. Two different animal models, F344 rats and A/J mice, were used to compare preneoplasia inhibition when fed one of three ...


Novel Mechanisms Involved With Lipid Metabolism In Adipose Tissue Of Dairy Cows, Dawn Koltes Jan 2013

Novel Mechanisms Involved With Lipid Metabolism In Adipose Tissue Of Dairy Cows, Dawn Koltes

Graduate Theses and Dissertations

Proper lipid metabolism is essential to the maintenance of milk production and fitness in the modern dairy cow. Little is known about the regulation of lipid metabolism in adipose tissue in dairy cattle, including the roles of adipokines and lipid droplet associated proteins. Therefore, the objectives of this study were to characterized changes in transcript abundances of adipokines during three different energy altering models, and lipid droplet associated proteins throughout the transition period and with leptin induced lipolysis. Transcript abundances of leptin decreased during feed restriction, while angiopoietin like protein 4 (ANGPTL4) increased during early lactation, feed restriction, and following ...


Performance, Carcass Traits And Fatty Acid Profiles Of Yearling Beef Cattle Supplemented With Self-Fed Byproducts On Pasture, Daniel Dean Kiesling Jan 2013

Performance, Carcass Traits And Fatty Acid Profiles Of Yearling Beef Cattle Supplemented With Self-Fed Byproducts On Pasture, Daniel Dean Kiesling

Graduate Theses and Dissertations

Due to rising costs of conventional feedstuffs, more research has been focused on feeding non-conventional feedstuffs, such as byproducts of ethanol production or further processing of grains (i.e. soyhulls). Because of the health benefits of conjugated linoleic acid (CLA) shown in studies on laboratory animals, consumer interest in foods enriched with CLA has increased. The objective of this study was to investigate the effects of finishing yearling market steers on pasture supplemented with combinations of self-fed byproducts on live animal performance, carcass traits and fatty acid profiles; specifically CLA. British and Continental crossbred beef steers (n = 162, BW = 404 ...


The Effect Of Supplemental Vitamin C On Performance, Antioxidant Capacity, Carcass Characteristics, And Meat Quality Of Steers Fed High Sulfur Finishing Diets, Danielle Jaye Pogge Jan 2013

The Effect Of Supplemental Vitamin C On Performance, Antioxidant Capacity, Carcass Characteristics, And Meat Quality Of Steers Fed High Sulfur Finishing Diets, Danielle Jaye Pogge

Graduate Theses and Dissertations

The incorporation of ethanol industry co-products, such as dried distillers grains plus solubles, to feedlot diets in the United States may be inadvertently exposing cattle to high amounts of dietary sulfur. High sulfur diets have repeatedly been reported to decrease growth and carcass performance, health, and copper status; however, little to no information is available concerning the implications that high sulfur diets have on the antioxidant capacity or meat quality of cattle. Thus, the subsequent research trials were designed to: 1) examine the impacts of high dietary S on diet digestibility and macro and micro mineral absorption and retention, 2 ...


Foraging By Mice In Spatially And Temporally Variable Agricultural Fields: Implications For Volunteer Corn, Invertebrate And Weed Pest Control, Tatyana Jennifer Flick Jan 2013

Foraging By Mice In Spatially And Temporally Variable Agricultural Fields: Implications For Volunteer Corn, Invertebrate And Weed Pest Control, Tatyana Jennifer Flick

Graduate Theses and Dissertations

The prairie deer mouse (Peromyscus maniculatus bairdii) is a common year-round resident of Midwestern agricultural fields, and provides several ecosystem services. Previous studies and preliminary findings have shown that the mouse consumes large quantities of weed seeds and waste grain during the fall and winter. However, it is unknown how the spatial and temporal variation in resource types and their distribution affects foraging by deer mice in these crop fields. The following two studies were designed to 1) experimentally test how resource distribution (patchiness) within a field affects foraging by deer mice and 2) examine the temporal variation in diet ...


Effects Of Diets Containing Digestion-Resistant Starch On Wnt Pathway Control Of Proliferation And Differentiation Of The Colorectal Mucosa, Nicole Cray Jan 2013

Effects Of Diets Containing Digestion-Resistant Starch On Wnt Pathway Control Of Proliferation And Differentiation Of The Colorectal Mucosa, Nicole Cray

Graduate Theses and Dissertations

Resistant starch (RS) is not digested in the small intestine and is available for fermentation by the colonic microbiota, producing metabolites including short chain fatty acids (SCFAs). We hypothesized that diets containing resistant starch, serving as a prebiotic, will have inhibitory effects, mediated through the products of microbial fermentation, on the canonical Wnt signaling pathway. To test this hypothesis, we examined the molecular and morphologic effects of diets containing resistant starches on the early stage of colorectal carcinogenesis in azoxymethane-induced rat and mouse models. The first study examined the effects of diets containing naturally occurring resistant starch from Latin American ...


Addressing Fatty Acids Toxicity And Production In Biocatalysts, Ping Liu Jan 2013

Addressing Fatty Acids Toxicity And Production In Biocatalysts, Ping Liu

Graduate Theses and Dissertations

Fatty acids recently gain more attentions due to its potentials as fuel and chemicals. However, they are toxic to biocatalysts like many other biofuel compounds. Understanding the inhibitory mechanism can help to improve the production of fatty acids.

In current study, the toxicity of short chain fatty acids: hexanoic, octanoic, and decanoic acid on Saccharomyces. cerevisiae and Escherichia.coli were inverstigated with a focus on octanoic acid. The toxicity of fatty acids (hexanoic, octanoic, and decanoic acid) in S. cerevisiae is dependent on doses and chain lengths, and also leads to damaged membrane intergirity, which was confirmed by increased membreane ...


Decoding Heterogeneous Big Data In An Integrative Way, Xia Zhang Jan 2013

Decoding Heterogeneous Big Data In An Integrative Way, Xia Zhang

Graduate Theses and Dissertations

Biotechnologies in post-genomic era, especially those that generate data in high-throughput, bring opportunities and challenges that are never faced before. And one of them is how to decode big heterogeneous data for clues that are useful for biological questions. With the exponential growth of a variety of data, comes with more and more applications of systematic approaches that investigate biological questions in an integrative way. Systematic approaches inherently require integration of heterogeneous information, which is urgently calling for a lot more efforts.

In this thesis, the effort is mainly devoted to the development of methods and tools that help to ...


Phylogeography Of Eastern Leatherwood (Dirca Palustris L.) Resolved By Chloroplast Sequencing And Microsatellite Genotyping, Bryan James Peterson Jan 2013

Phylogeography Of Eastern Leatherwood (Dirca Palustris L.) Resolved By Chloroplast Sequencing And Microsatellite Genotyping, Bryan James Peterson

Graduate Theses and Dissertations

The broad distribution of eastern leatherwood (Dirca palustris L.), from Florida to Canada, contrasted with its patchy restriction to rich, mesic forests and the paucity of vectors for dispersal of its seeds, represents a classic example of Reid's paradox of plant migration. It long has been presumed that temperate forest species of eastern North America persisted in southern glacial refugia near the Gulf Coast, after which many dispersed nearly 2000 km to contemporary northern range limits. The rates of migration necessary to account for present distributions exceed those suggested by contemporary observations of seed dispersal. I used ecological niche ...


Understanding Biodiversity: The Importance Of Sexual Dimorphism In The Micro- And Macroevolution Of Hummingbirds, Chelsea Marie Berns Jan 2013

Understanding Biodiversity: The Importance Of Sexual Dimorphism In The Micro- And Macroevolution Of Hummingbirds, Chelsea Marie Berns

Graduate Theses and Dissertations

ABSTRACT

For centuries, biologists have asked "why are there so many species, and why do we see such magnificent biological diversity?" This dissertation addresses this question by examining sexual size and shape dimorphism hummingbird bills at both a micro and macroevolutionary scale. Specifically, I quantify sexual size and shape dimorphism of bill morphology of 269 species of hummingbirds and begin with a species-level study to find that community structure might be a driving factor in the evolution of sexual size dimorphism in 2 sister species of hummingbirds, and the presence of sexual shape dimorphism in one of those species.

I ...


Measuring Femoral Neck Loads In Older Adults During Stair Ascent And Descent, Chen Deng Jan 2013

Measuring Femoral Neck Loads In Older Adults During Stair Ascent And Descent, Chen Deng

Graduate Theses and Dissertations

ABSTRACT

Fractures at the femoral neck play an important role in morbidity and mortality among older adults. Understanding the loading environment is a crucial factor for reducing the incidence of fracture at this site. In this study, the stresses on the femoral neck during stair ascent and descent were estimated. These femoral neck stresses were also compared to the peak loading from internal hip moments. Five male and five female adult subjects performed 5 successful trials of stair ascent and the same number trials of descent with a 3-step staircase. Motion and force data were collected. Inverse dynamics was used ...


Oral S-Adenosyl Methionine (Sam) Mediates Disruptions In Methyl Group Metabolism Due To Retinoic Acid Therapy And Alters Neurotransmitter Metabolism: Implications For Major Depressive Disorder, Anne Smazal Jan 2013

Oral S-Adenosyl Methionine (Sam) Mediates Disruptions In Methyl Group Metabolism Due To Retinoic Acid Therapy And Alters Neurotransmitter Metabolism: Implications For Major Depressive Disorder, Anne Smazal

Graduate Theses and Dissertations

Disruptions in methyl group metabolism have been associated with a number of disease states, including birth defects, cardiovascular disease, and neurological disorders. Therefore, characterization of the factors that regulate folate and methyl group supply is essential for the development of disease treatments and prevention. Glycine N-methyltransferase (GNMT), an enzyme essential to methyl group balance, is markedly increased by retinoic acid treatment. Furthermore, retinoid therapy has been shown to impact neurotransmitter metabolism, thereby contributing to the development of major depressive disorder. Recently, it has been shown that depressed patients have low levels of folate, other B-vitamins, and s-adenosylmethionine (SAM), all compounds ...


Use Of Stable Isotope And Trace Metal Signatures To Track The Emigration Of Female Blue Crabs, Callinectes Sapidus, From Tampa Bay, Sky Barrington Williams Jan 2013

Use Of Stable Isotope And Trace Metal Signatures To Track The Emigration Of Female Blue Crabs, Callinectes Sapidus, From Tampa Bay, Sky Barrington Williams

Graduate Theses and Dissertations

The blue crab, Callinectes sapidus, supports a successful fishery in the Atlantic Ocean and throughout the Gulf of Mexico, with a total landing of 8,158,788 lb. and a total value of $10,562,128 for the state of Florida during 2012 (FWC 2012 Annual Landings Summary). An accurate and comprehensive understanding of the blue crab's life history and seasonal migration behavior is essential in defining effective management strategies for the fishery. Tag recapture studies and ultrasonic tracking methods for studying blue crab migrations are costly in terms of time and resources. In this study an alternative approach ...


Biochemical Aspects Of The Thermal Sensitivity And Energy Balance Of Polar, Tropical And Subtropical Teleosts, Eloy Martinez Jan 2013

Biochemical Aspects Of The Thermal Sensitivity And Energy Balance Of Polar, Tropical And Subtropical Teleosts, Eloy Martinez

Graduate Theses and Dissertations

The maintenance of a functional energy balance in ectothermic fauna could be challenging in a thermally disparate environment. Biochemical adaptations at the enzyme and membrane levels allows for a set compensatory mechanism that allow the individual to maintain an energetic surplus, thus allocating energy for growth and reproduction. The present work describes how the energetic machinery in the cell, particularly the mitochondrion, could be affected by temperature changes. More specifically, this work aimed to determine how environmental temperature affects the mitochondria energetic performance of fishes from disparate thermal regimes.

Mitochondrial ATP production efficiency was evaluated in fishes from polar, tropical ...


Regulation Of The Tumor Suppresser P53 And Survivin By Ras And Ral Gtpases:Implications For Malignant Transformation, Awet G. Tecleab Jan 2013

Regulation Of The Tumor Suppresser P53 And Survivin By Ras And Ral Gtpases:Implications For Malignant Transformation, Awet G. Tecleab

Graduate Theses and Dissertations

Abstract

Although the critical role of the small GTPases Ras and Ral in oncogenesis has been well documented, much remains to be investigated about the molecular mechanism by which these GTPases regulate malignant transformation. The work under this thesis made two major contributions to this field. The first is the discovery that K-Ras, RalA and/or RalB are required for the maintenance of the high levels of the anti-apoptotic protein survivin in some human cancer cells, and the second is the demonstration that down regulation of K-Ras, RalA and/or RalB, but not Raf-1 or Akt1/2, stabilizes the tumor ...


Computational Approaches For Structure Based Drug Design And Protein Structure-Function Prediction, Sai Lakshmana Kumar Vankayala Jan 2013

Computational Approaches For Structure Based Drug Design And Protein Structure-Function Prediction, Sai Lakshmana Kumar Vankayala

Graduate Theses and Dissertations

This dissertation thesis consists of a series of chapters that are interwoven by solving interesting biological problems, employing various computational methodologies. These techniques provide meaningful physical insights to promote the scientific fields of interest. Focus of chapter 1 concerns, the importance of computational tools like docking studies in advancing structure based drug design processes. This chapter also addresses the prime concerns like scoring functions, sampling algorithms and flexible docking studies that hamper the docking successes. Information about the different kinds of flexible dockings in terms of accuracy, time limitations and success studies are presented. Later the importance of Induced fit ...


The Mevalonate Pathway: A Potential Therapeutic Target For Jak2-Driven Myeloproliferative Neoplasms, Lori Nicole Griner Jan 2013

The Mevalonate Pathway: A Potential Therapeutic Target For Jak2-Driven Myeloproliferative Neoplasms, Lori Nicole Griner

Graduate Theses and Dissertations

The Mevalonate Pathway: A Potential Therapeutic Target for JAK2-driven Myeloproliferative Neoplasms

Lori Nicole Griner

Abstract

Myeloproliferative neoplasms (MPNs) are diseases of hematopoietic stem cell origin and are characterized by uncontrolled growth of cells of the myeloid compartment. The Philadelphia chromosome negative classical MPNs, including polycythemia vera, essential thrombocythemia, and myelofibrosis, are diseases of dysregulated JAK2 signaling. In fact, the majority of MPN patients have activating mutations in JAK2 (e.g JAK2-V617F), a tyrosine kinase that contributes to the growth and survival of myeloid cells. While MPNs were first described over sixty years ago, a significant need remains to develop therapeutic ...


Development Of A Corn Stover Yield Monitor, Byron Manternach Jan 2013

Development Of A Corn Stover Yield Monitor, Byron Manternach

Graduate Theses and Dissertations

This research developed a sensor to measure the mass flow of corn stover through a combine and determine what harvest parameters affected the response of the sensor. A sensor was developed by installing a curved plate behind the rotor discharge beater of the combine to intersect the flow path of the stover leaving the rotor discharge beater. The plate was instrumented with load cells to measure the impact force of the corn stover on the impact plate. Instrumentation methods for the impact plate were evaluated to determine the most effective method to instrument the impact plate to measure the impact ...


Iron Bioavailability And Consumer Acceptability Of Extruded Common Bean (Phaseolus Vulgaris) Flour, Martin Mutambuka Jan 2013

Iron Bioavailability And Consumer Acceptability Of Extruded Common Bean (Phaseolus Vulgaris) Flour, Martin Mutambuka

Graduate Theses and Dissertations

Iron deficiency anemia continues to be a major nutritional challenge worldwide and is mainly caused by dependence on staple foods which are low in iron bioavailability. In many parts of the world, the common bean (Phaseolus vulgaris) is an important source of iron. Bean iron has very low bioavailability and household/industrial processing technologies have been shown to improve iron bioavailability. In this study, iron bioavailability of 16 Ugandan bean varieties was determined using an in vitro digestion/Caco-2 cell culture model and modeled with respect to key influencing factors; phytate, polyphenol, ferritin and iron content. The effect of extrusion ...


Genetic And Phenotypic Relationships Between Feed Intake During Lactation And Reproductive Performance, Chad Lewis Yoder Jan 2013

Genetic And Phenotypic Relationships Between Feed Intake During Lactation And Reproductive Performance, Chad Lewis Yoder

Graduate Theses and Dissertations

Daily feed intake during lactation was recorded on purebred Yorkshire (n = 1587), Landrace (n = 2197), and F1 Yorkshire x Landrace (n = 6932) litters from day 1 to 22 of lactation. Lactation feed intake (LFI) curves were predicted using a mixed model which included fixed effects of breed, season, parity group (PG), day of lactation, interactions of day with breed and PG, and a covariate for litter size after cross–fostering. Random effects included litter, contemporary group (herd–year–month), dam, and sire nested within breed. Least squares means for each day were used to define LFI curves by breed through ...


Molecular Regulation Of Il-2 Inducible T-Cell Kinase (Itk) And The Tec Kinases: A Combined Experimental And Computational Study, With Emphasis On The N-Terminal Pleckstrin Homology Domain, Scott Edward Boyken Jan 2013

Molecular Regulation Of Il-2 Inducible T-Cell Kinase (Itk) And The Tec Kinases: A Combined Experimental And Computational Study, With Emphasis On The N-Terminal Pleckstrin Homology Domain, Scott Edward Boyken

Graduate Theses and Dissertations

This dissertation investigates the complex regulation of IL-2 inducible T-cell kinase (Itk) and its related Tec family kinase members, using a combination of experimental and computational approaches. Tec kinases are expressed primarily in hematopoietic cells and regulate key signaling events in lymphocyte activation, differentiation, and development. Itk functions in immune response, fine-tuning signaling downstream of the T-cell Receptor (TCR), and is a putative drug target for allergies and autoimmune disease. Btk, a Tec kinase found in B-cells, carries out a similar role to that of Itk, only downstream of B-cell Receptor (BCR) signaling. The chapters of this dissertation focus on ...


Calibration Optimization And Efficiency In Near Infrared Spectroscopy, Nanning Cao Jan 2013

Calibration Optimization And Efficiency In Near Infrared Spectroscopy, Nanning Cao

Graduate Theses and Dissertations

Calibration optimization in near infrared spectroscopy (NIRS) is a complex process, requiring long-term database maintenance and model update by including new variations. A sample selection procedure was introduced to identify the number and choice of samples required in a NIRS calibration model. The example case is the determinations of moisture, protein and oil contents in whole soybeans. The original large database is composed of soybean NIR transmittance spectra (n>8,000) across crop years (2001-2011), varieties and locations. Uniform random, Kennard-Stone and D-optimal algorithms were compared for calibration sample selection. The optimal models based on calibration set selected by uniform ...


Development Of A Protein Antigen Based Pneumococcal Vaccine Utilizing A Polyanhydride Nanoparticle Delivery Platform, Amy D. Schoofs Jan 2013

Development Of A Protein Antigen Based Pneumococcal Vaccine Utilizing A Polyanhydride Nanoparticle Delivery Platform, Amy D. Schoofs

Graduate Theses and Dissertations

Streptococcus pneumoniae is the primary cause of bacterial pneumonia and contributes to millions of deaths worldwide annually. The current capsule polysaccharide based vaccines do provide some protection, but the 7 to 23 valent formulations cannot protect against the over 90 serotypes currently known. Pneumococcal surface protein A (PspA) is a promising candidate for a protein-based vaccine against S. pneumoniae, showing protection in immunized mice and providing the advantage of universal protection from all serotypes of the bacterium. This project shows the results of immunizing CBA/N mice intranasally and subcutaneously with PspA encapsulated into a novel polyanhydride nanoparticle delivery platform ...


Correction For Rapid-Growth Vegetation And Testing Of An Upscaling Method With A Cosmos Probe, Samantha Lou Irvin Jan 2013

Correction For Rapid-Growth Vegetation And Testing Of An Upscaling Method With A Cosmos Probe, Samantha Lou Irvin

Graduate Theses and Dissertations

COSMOS (COsmic-ray Soil Moisture Observing System) probes are a developing instrument in the field of remote sensing and are currently the only source of field scale soil moisture data. A COSMOS probe is essentially a hydrogen particle counter with a measurement depth ranging from 10-70 cm and a spatial resolution approaching 700 m in diameter. A majority of the hydrogen detected is in the form of water molecules. As vegetation contains both hydrogen in the vegetative dry matter and water, in addition to sitting above the ground, it may influence what is measured by the COSMOS probe as soil moisture ...


Experimentally Simulating Environmental Change In A Montane Meadow System Via Reduced Snowpack And Passive Warming: Soil And Plant Responses, Jill Sherwood Jan 2013

Experimentally Simulating Environmental Change In A Montane Meadow System Via Reduced Snowpack And Passive Warming: Soil And Plant Responses, Jill Sherwood

Graduate Theses and Dissertations

Global and regional climate patterns suggest that the environment in the western United States is trending towards warmer and drier conditions. The ecological effects of climate change could include shifts in distribution and phenological changes for many plants and animals. This study provides an initial examination of climatic and phenological (timing of key biological events) trends in an effort to understand how montane plants may be affected by climatic changes. To explore the consequences of increased temperatures and decreased snow cover, we conducted an experiment in a montane meadow where snow removal and passive heating were used to mimic the ...


Phenotypic Consequences Of Maternal Effects During Early Life Stages Of The Painted Turtle, Chrysemys Picta, Timothy Staub Mitchell Jan 2013

Phenotypic Consequences Of Maternal Effects During Early Life Stages Of The Painted Turtle, Chrysemys Picta, Timothy Staub Mitchell

Graduate Theses and Dissertations

Maternal effects are a ubiquitous source of phenotypic variation and relevant to understanding many ecological and evolutionary questions. For oviparous organisms, egg size and oviposition/ nest-site choice are two maternal effects that can be particularly important. A classic assumption in life-history theory is that offspring with more resources allocated to them have a fitness advantage, but this assumption is challenging to assess in the wild. Oviposition or nest-site choice can influence many aspects of the offspring environment including abiotic conditions, competition, predation risk, and food availability.

This dissertation focuses on the influence of egg size and nest-site choice on early ...


Design Of Novel Nano-Carriers For Multi-Enzyme Co-Localization, Feng Jia Jan 2013

Design Of Novel Nano-Carriers For Multi-Enzyme Co-Localization, Feng Jia

Graduate Theses and Dissertations

The widely existing MECs in Nature have inspired researchers to design synthetic analogs to promote the overall catalytic efficiency in vitro by co-localizing multiple enzymes to mimic the MECs' unique functionalities. A number of efforts have been devoted to designing synthetic MECs in the past couples of decades. This thesis work has focused on developing novel strategies based on enzyme immobilization to design nano-carriers for multi-enzyme co-localization to realize kinetics enhancement and strong control of spatial arrangement of the enzymes. Three distinct approaches have been designed using different attachment methods and platforms.

First, the multifunctional polystyrene nanoparticles were designed for ...