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Full-Text Articles in Life Sciences

Regulating Rsma Expression In Pseudomonas Aeruginosa, Sean D. Stacey Aug 2013

Regulating Rsma Expression In Pseudomonas Aeruginosa, Sean D. Stacey

Electronic Theses and Dissertations

Pseudomonas aeruginosa, a Gram-negative bacillus, commonly infects immunocompromised individuals and uses a variety of virulence factors to persist in these hosts. The posttranscriptional regulator, RsmA, plays a role in the expression of many virulence factors in P. aeruginosa. RsmA up regulates virulence factors used in colonizing hosts. However, regulation of rsmA is not well elucidated. Transposon mutagenesis was performed on P. aeruginosa containing a transcriptional rsmA-lacZ fusion to answer this question. Mutants were screened via β-galactosidase assay and transposon insertions identified via arbitrary PCR. A probable MFS transporter, we named mtpX, was one significant transposon mutant identified. A ΔmtpX mutant ...


Exercise Participation During Weight Loss On A High Protein – Low Carbohydrate Diet Plan In Females Aged 15-25 Years, Margaret Mobley-Meulman Aug 2013

Exercise Participation During Weight Loss On A High Protein – Low Carbohydrate Diet Plan In Females Aged 15-25 Years, Margaret Mobley-Meulman

Electronic Theses and Dissertations

Weight gain due to poor diet and lack of exercise is responsible for over 300,000 deaths each year (U.S. Department of Health and Human Services, 2010). Obese adults have an increased risk for serious health conditions including high blood pressure and cholesterol, type 2 diabetes, coronary heart disease, stroke, gallbladder disease, osteoarthritis, sleep apnea, respiratory problems, and certain cancers (National Cancer Institute, 2012). Participation in exercise can help control weight, strengthen muscles and bones, and reduce the incidence of cardiac events, stroke, hypertension, type 2 diabetes, colon and breast cancers, osteoporotic fractures, gallbladder disease, obesity, depression, anxiety, and ...


Bioengineering The Expression Of Active Recombinant Human Cathepsin G, Enteropeptidase, Neutrophil Elastase, And C-Reactive Protein In Yeast, Eliot T. Smith Aug 2013

Bioengineering The Expression Of Active Recombinant Human Cathepsin G, Enteropeptidase, Neutrophil Elastase, And C-Reactive Protein In Yeast, Eliot T. Smith

Electronic Theses and Dissertations

The yeasts Pichia pastoris and Kluyveromyces lactis were used to express several recombinant human proteins for further biochemical characterization. Two substitution variants of recombinant human enteropeptidase light chain (rhEPL) were engineered to modify the extended substrate specificity of this serine protease. Both were secreted as active enzymes in excess of 1.7 mg/L in P. pastoris fermentation broth. The substitution variant rhEPL R96Q showed significantly reduced specificities for the preferred substrate sequences DDDDK and DDDDR; however, the rhEPL Y174R variant displayed improved specificities for these substrate sequences relative to all other reported variants of this enzyme. The neutrophil serine ...


Building A Foundation For Interprofessional-Education (Ipe) Between Dietetic Students And Dental Hygiene Students At East Tennessee State University (Etsu), Monique Richard May 2013

Building A Foundation For Interprofessional-Education (Ipe) Between Dietetic Students And Dental Hygiene Students At East Tennessee State University (Etsu), Monique Richard

Electronic Theses and Dissertations

Nutrition education is an integral part of dental education as well as a variety of other healthcare professions1, but interprofessional education (IPE) between the fields of dietetics and dental hygiene is limited. The purpose of this pilot study was to define areas of opportunity to establish a foundation for the implementation of complimentary curriculum between the dietetics and dental hygiene programs at ETSU. A 76-question survey was developed and administered to dietetic interns (n=26), dental hygiene students (n=49), dietetic faculty (n=23), and dental hygiene faculty (n=19) at ETSU and Baylor College of Dentistry at Texas ...


Genetic Imbalances In Endometriosis Detected By Oligonucleotide-Array Based Comparative Genomic Hybridization, Natalie Burke May 2013

Genetic Imbalances In Endometriosis Detected By Oligonucleotide-Array Based Comparative Genomic Hybridization, Natalie Burke

Electronic Theses and Dissertations

Endometriosis is one of the most common gynecological diseases as it is thought to affect up to 15% of the female population. Characterized by the growth and proliferation of endometrial tissue outside of the uterine cavity, it is a complex condition with varying degrees of severity and can affect multiple regions of the body with symptoms ranging from a total lack of symptoms to debilitating pain and infertility. The most accepted theory of how endometriosis initiates is that of retrograde menstruation; however, approximately 90% of women with unobstructed fallopian tubes are thought to have some menstrual debris in the peritoneal ...


A Mathematical Model For Antibiotic Resistance In A Hospital Setting With A Varying Population, Edward H. Snyder May 2013

A Mathematical Model For Antibiotic Resistance In A Hospital Setting With A Varying Population, Edward H. Snyder

Electronic Theses and Dissertations

Antibiotic-resistant bacteria(ARB) is causing increased health risk and cost to society. Mathematical models have been developed to study the transmission of resistant bacteria and the efficacy of preventive measures to slow its spread within a hospital setting. The majority of these models have assumed a constant total hospital population with the admission and discharge rates being equal throughout the duration. But a typical hospital population varies from day to day and season to season. In this thesis, we apply variable admission and discharge daily rates to existing deterministic and stochastic models which examine the transmission of single and dual ...


Concussion Knowledge And Attitudes In English Football (Soccer), Joshua Williams Apr 2013

Concussion Knowledge And Attitudes In English Football (Soccer), Joshua Williams

Electronic Theses and Dissertations

While concussions are prevalent in soccer, outside the US there has been limited investigation into soccer players' knowledge and attitudes regarding concussions. The purpose of the study was to access English soccer player' concussion knowledge and attitudes. This study used a cross sectional mixed methodology design. Participants included 26 respondents (Age: 23.4 ± 4.5 years, playing experience 16.4 ± 4.4 years) playing for a Championship team in England (Response rate: 89.6%). Fifty percent of respondents had a diagnosed concussion history. Participants were administered the valid and reliable ROCKAS-ST survey divided into 5 sections and includes 3 constructs ...


Ncaa Division I Head Coaches' Experiences With Eating Disorders And Disordered Eating In Female Athletes: A Qualitative Analysis, Caitlyn Pecinovsky Apr 2013

Ncaa Division I Head Coaches' Experiences With Eating Disorders And Disordered Eating In Female Athletes: A Qualitative Analysis, Caitlyn Pecinovsky

Electronic Theses and Dissertations

In today's sport, NCAA Division I female athletes are under a constant pressure to reach an elite level of perfection in athletic performance, frequently adhering to the assumption that there is an inverse relationship between body size and performance level (Petrie & Sherman, 2007). Additionally, coaches have unrealistic expectations about weight and body image, which adds pressure on athletes to conform to certain weight or image driven standards. Perceptions that coaches hold about eating disorders and disordered eating present a set of risk factors that may increase the likelihood of female athletes developing maladaptive eating patterns (Scoffier, Maiano, & d'Arripe-Longueville ...


Physiological Muscle Qualitative Changes In Response To Resistance Training In Older Adults, Tyler Scanlon Jan 2013

Physiological Muscle Qualitative Changes In Response To Resistance Training In Older Adults, Tyler Scanlon

Electronic Theses and Dissertations

Muscle function is determined by structure and morphology at the architectural level. In response to resistance training, older adults have demonstrated that the neuromuscular system has a substantial adaptability, which may compensate for muscle size and quality and lead to improved functional capacities and higher quality of life. PURPOSE: The purpose of this study was to examine the effect of six weeks of progressive resistance exercise on muscle morphology and architecture in healthy older adults. METHODS: Twenty- five healthy men and women were randomly assigned to either six weeks of progressive resistance training (RT) (n=13; age = 71.08 ± 6 ...


The Effects Of 6-Weeks Of Resistance Training On The Neuromuscular Fatigue Threshold In Older Adults, Nadia Emerson Jan 2013

The Effects Of 6-Weeks Of Resistance Training On The Neuromuscular Fatigue Threshold In Older Adults, Nadia Emerson

Electronic Theses and Dissertations

Age-related deficits in muscle mass, strength, and function place an increased burden of work on existing skeletal muscle and may lead to early onset of neuromuscular fatigue (NMF) during activities of daily living. Resistance exercise (RE) is the proven method for improving neuromuscular function in healthy older adults. PURPOSE: To investigate the effects of 6 weeks of RE on the NMF threshold as well as strength and functional performance in older adults. METHODS: Twenty-four older adults were randomly assigned to 6 weeks of RE (EXE; n = 12; age 72 ± 6.3 y; BMI 28.4 kg/m2 ) or control (CONT ...


Do Changes In Muscle Architecture Effect Post- Activation Potentiation, Danielle Reardon Jan 2013

Do Changes In Muscle Architecture Effect Post- Activation Potentiation, Danielle Reardon

Electronic Theses and Dissertations

Purpose: To examine the effect of three muscle potentiation protocols on changes in muscle architecture and the subsequent effect on jump power performance. Methods: Maximal (1RM) squat strength (Mean SD=178.3 ± 36.6kg), vertical jump power, and muscle architecture were obtained in 12 resistance trained men (25.2±3.6y; 90.67±12.7kg). Participants randomly completed three squatting protocols at 75% (3 x 10 reps), 90% (3 x 3 reps) or 100% (1 x 1) of their 1RM, or no workout (CON), with each protocol being separated by one week. During each testing session ultrasound and vertical jump ...


Concussion Knowledge In Amateur Motocross, Kristina Miller Jan 2013

Concussion Knowledge In Amateur Motocross, Kristina Miller

Electronic Theses and Dissertations

The Center for Disease Control and Prevention estimated that there are 1.6-3.8 million sports related traumatic brain injuries in the United States annually which occur in both mainstream and extreme sports. Many of these concussions have been associated with collisional sports, like football, but high risk extreme sports, like motocross, also have many opportunities for collisions to occur. Unlike traditional varsity sports motocross races, especially at the amateur level, do not require more than advanced life support at a race in case of emergency situation leaving the amateur rider to recognize and decide on concussion management. Purpose of ...


Choice Response Time Differences Between Recently Recovered Concussed And Healthy Student-Athletes, Tiffen Tapia-Lovler Jan 2013

Choice Response Time Differences Between Recently Recovered Concussed And Healthy Student-Athletes, Tiffen Tapia-Lovler

Electronic Theses and Dissertations

Some 1.6 to 3.8 million sports-related concussions occur annually in the United States. Utilization of test batteries and exercise protocols are recommended to ensure athletes recover completely. Many batteries involve response time (RT) tests, which show response time increases post concussion. A major limitation of RT tests is that all are done in static position. Additionally, many studies show a lingering effect on RT. The addition of RT tests to check for lingering symptoms could be beneficial. The purpose of this study was to see if significant differences could be found in RT tests involving dynamic movement between ...