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Theses/Dissertations

2013

All Graduate Theses and Dissertations

Molecular Biology

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Full-Text Articles in Life Sciences

Evaluation Of The Tgf-ß Inhibitor Repsox On The Expression Of Pluripotency Pathways In Murine And Bovine Cells, Davin M. Larsen May 2013

Evaluation Of The Tgf-ß Inhibitor Repsox On The Expression Of Pluripotency Pathways In Murine And Bovine Cells, Davin M. Larsen

All Graduate Theses and Dissertations

Embryonic stem cells are pluripotent cells isolated from morula stage embryos or the inner cell mass of blastocyst stage embryos. They are capable of differentiating into tissues of all three primary germ layers. In recent years pluripotent cell lines have been created from somatic cell types using various methods, the primary method being viral transduction of exogenous Oct4, Sox2, Klf4, and c-Myc or Oct4, Sox2, Nanog, and Lin28 transgene constructs. The resulting cell lines are termed induced pluripotency stem cells, and are similar to embryonic stem cells in many ways. However, these cell lines are not acceptable for clinical applications ...


Characterization And Potential Utility Of Porcine Trophoblast-Derived Stem-Like Cells, Edison A. Suasnavas May 2013

Characterization And Potential Utility Of Porcine Trophoblast-Derived Stem-Like Cells, Edison A. Suasnavas

All Graduate Theses and Dissertations

In mammals, the trophoblast lineage of the embryo is specified before implantation. It is restricted to become the fetal portion of the placenta. We have isolated and cultured trophoblast-derived cells from day 10 and day 13 porcine embryos. These cells demonstrate morphological and biological characteristics that make them unique. We have demonstrated that these cells can grow in vitro in a defined, serum-replacement medium for over a year without showing any signs of senescence. Trophoblast-derived cells placed into serum-containing medium, however, rapidly senesce and fail to proliferate. Gene expression analysis by RT-PCR and Fluidigm analysis of cells in culture from ...