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Full-Text Articles in Life Sciences

Determination Of The Expression Patterns Of Bovine Non-Classical Major Histocompatibility Complex (Mhc) Class I Proteins, Parveen Parasar Dec 2013

Determination Of The Expression Patterns Of Bovine Non-Classical Major Histocompatibility Complex (Mhc) Class I Proteins, Parveen Parasar

All Graduate Theses and Dissertations

My dissertation hypothesis is that bovine trophoblast cells express cell-surface and secreted non-classical major histocompatibility complex class I (MHC-Ib) proteins which inhibit NK cells and other leukocytes by binding to inhibitory receptors (e.g., LILRB1, LILRB2, KIR2DL4, and/or CD94/NKG2A).

Extremely polymorphic and ubiquitously expressed classical MHC class I (MHC-Ia) proteins, which present foreign antigenic peptides to CD8+ T lymphocytes, are involved in acceptance or rejection of tissue grafts. Non-classical MHC class I (MHC-Ib) glycoproteins, such as Human Leukocyte Antigen-G (HLA-G) and murine Qa-2, are important modulators of the maternal immune system during pregnancy. MHC-Ib proteins are: (a) oligomorphic ...


Regulation And Expression Of Nanog, Oct4, And Sox2 In The Bovine Blastocyst Following Somatic Cell Nuclear Transfer, Justin Scott Hall May 2013

Regulation And Expression Of Nanog, Oct4, And Sox2 In The Bovine Blastocyst Following Somatic Cell Nuclear Transfer, Justin Scott Hall

All Graduate Theses and Dissertations

A live birth from a somatic cell nuclear transfer (SCNT) embryo represents a small percentage of donor cells that survived the reprogramming gauntlet. The inability to reprogram histone modifications in the donor cell line could add to the reprogramming deficiencies associated with SCNT. The effects of two histone modifications associated with transcriptional activation (H3K4m3 and H4K16ac) and two histone modifications associated with repressing transcription (H3K9m2 and H3K27me3) were evaluated in the context of their association to three genes known to contribute to maintaining totipotency: Nanog, Oct4, and Sox2. A µChIP assay was utilized using antibodies specific for each histone modification ...


The Micronutrient Profile Of The Typical American Diet Enhances Colorectal Carcinogenesis, Stephany Del Carmen Perez Monsanto May 2013

The Micronutrient Profile Of The Typical American Diet Enhances Colorectal Carcinogenesis, Stephany Del Carmen Perez Monsanto

All Graduate Theses and Dissertations

The typical Western dietary pattern is characterized by the consumption of energy-dense, nutrient-poor foods and has been linked to increased risk of colorectal cancer (CRC). Our research group previously developed the total Western diet (TWD) that emulates typical human dietary intakes of macro- (carbohydrates, proteins, and fats) and micronutrients (vitamins and minerals) on an energy density basis for rodents. In the present study, we sought to determine the impact of TWD on biomarkers of metabolic syndrome and obesity in comparison to a commercial 45% fat diet used for models of diet-induced obesity (DIO diet) and the standard basal AIN93G diet ...


Analysis Of Single Nucleotide Polymorphism Panels For Bovine Dna Identification, Kimberly A. Blanchard May 2013

Analysis Of Single Nucleotide Polymorphism Panels For Bovine Dna Identification, Kimberly A. Blanchard

All Graduate Theses and Dissertations

Single nucleotide polymorphisms (SNPs) are single base-pair variations that exist between individuals. There are approximately a million or more SNPs located throughout the genome of each individual animal. Therefore, by taking advantage of these unique polymorphisms, SNPs can be used to resolve questions of unknown parentage in the livestock industry. Currently a panel of 88 SNPs, obtained from a panel of 121 SNPs originally created by USDA-MARC, is commercially available from Fluidigm®. The objective of this study was to determine whether the number of SNPs from the 88-SNP marker panel could be reduced to form a smaller, more cost-efficient parentage-testing ...