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Retroviral Mutagenesis In A Newly Developed Myc Transgenic Mouse Model Of Human B Cell And Plasma Cell Neoplasia, Samuel Robert Lifton Dec 2013

Retroviral Mutagenesis In A Newly Developed Myc Transgenic Mouse Model Of Human B Cell And Plasma Cell Neoplasia, Samuel Robert Lifton

Theses and Dissertations

Three potential driver genes were identified by use of retroviral mutagenesis in the newly developed iMyc model of B cell malignancy. To do so, iMyc mice, which bear an inserted copy of c-Myc in the IgH locus, were treated with MOL4070LTR to favor the development of B cell malignancies. After tumor development, B cell origin tumors were identified by use of immunohistochemistry and selected for downstream analysis. Three genes were chosen as potential driver genes and validated in mouse or human disease as involved in disease or directly in malignancy. These genes are CD82, IRAK2 and DNMT3a.


Ear Manipulations Help Model Neuroplasticity Limitations, Karen Louise Elliott Thompson Dec 2013

Ear Manipulations Help Model Neuroplasticity Limitations, Karen Louise Elliott Thompson

Theses and Dissertations

Sensory organs, such as the inner ear, send information about the outside world to the central nervous system (CNS) through afferent neurons and in turn, the CNS sends information back to certain sensory organs through efferent neurons to modulate the incoming signal. Though how these afferent and efferent neurons navigated with their processes to the hindbrain or hair cells, respectively, is not clear. By transplanting ears to other locations, or adding ears, we can effectively create a novel ear to ask how the CNS adapts to a new sensory system, complete with efferent innervation of hair cells and afferent innervation ...


The Roles Of N-Myc And L-Myc During Inner Ear Neurosensory Development, Benjamin Joseph Kopecky Dec 2013

The Roles Of N-Myc And L-Myc During Inner Ear Neurosensory Development, Benjamin Joseph Kopecky

Theses and Dissertations

Introduction: Hearing loss affects over 500 million people worldwide and results from irreversible damage to inner ear hair cells. The only available treatment is cochlear implants, which may be unable to provide sensory input if neuronal connections are lost, as they are in mouse models. Thus, regeneration of hair cells offers the only permanent cure; however, such therapeutic intervention requires a detailed molecular understanding inner ear development and hair cell maintenance. During mouse development, there is a balance between proliferation and differentiation that not only determines the size of the ear, but also is needed to form a functional sensory ...


Elucidating Mechanisms By Which Substance P In The Rvm Contributes To The Maintenance Of Pain Following Inflammatory Injury, Uche Patrick Maduka Dec 2013

Elucidating Mechanisms By Which Substance P In The Rvm Contributes To The Maintenance Of Pain Following Inflammatory Injury, Uche Patrick Maduka

Theses and Dissertations

Chronic pain is a major healthcare concern that directly affects over one hundred million people in the United States alone. While current treatment options like opioids and NSAIDs are effective, they are with significant drawbacks that prevent long term use. It is important to identify and understand new druggable targets for the treatment of pain. Recent findings have demonstrated substance P functions in the RVM to maintain hypersensitivity to noxious heat stimuli in models of persistent peripheral inflammatory injury in a manner dependent on presynaptic NMDA receptors. What remains unclear is how substance P assumes this pronociceptive role following peripheral ...


Characterization Of Cis-Acting Partners Within The Cytomegalovirus Major Immediate-Early Enhancer That Strengthen Mie Gene Expression And Viral Fitness, Courtney Searcey Galle Dec 2013

Characterization Of Cis-Acting Partners Within The Cytomegalovirus Major Immediate-Early Enhancer That Strengthen Mie Gene Expression And Viral Fitness, Courtney Searcey Galle

Theses and Dissertations

Human cytomegalovirus infects approximately 50% of adults in the United States and in most cases is asymptomatic. However, in the case of immune compromised persons such as AIDS patients, transplant patients, and newborn babies, life threatening CMV disease can occur. The HCMV major immediate-early enhancer functions as a master regulatory switch, whose activation is essential for the expression of the major IE transactivating proteins, IE1 p72 and IE2 p86. While critical to the viral lifecycle, regulation of MIE enhancer activation is very complex and not yet fully understood. I characterized the role of cis-acting partners within the MIE enhancer that ...


A Novel Role For Prolyl-Hydroxylase 3 Gene Silencing In Epithelial-To-Mesenchymal-Like Transition, Trenton Lane Place Dec 2013

A Novel Role For Prolyl-Hydroxylase 3 Gene Silencing In Epithelial-To-Mesenchymal-Like Transition, Trenton Lane Place

Theses and Dissertations

The ability of cells to sense oxygen is a highly evolved process that facilitates adaptations to the local oxygen environment and is critical to energy homeostasis. In vertebrates, this process is largely controlled by three intracellular prolyl-4-hydroxylases (PHD 1-3). These related enzymes share the ability to hydroxylate the hypoxia-inducible transcription factor (HIF), and therefore control the transcription of genes involved in metabolism and vascular recruitment. However, it is becoming increasingly apparent that proline-4-hydroxylation controls much more than HIF signaling, with PHD3 emerging as the most unique and functionally diverse of the PHD isoforms. In fact, PHD3-mediated hydroxylation has recently been ...


Cell Biological Defects In Juvenile Neuronal Ceroid Lipofuscinosis, Mark Schultz Dec 2013

Cell Biological Defects In Juvenile Neuronal Ceroid Lipofuscinosis, Mark Schultz

Theses and Dissertations

Mutations in the CLN3 gene cause Juvenile Neuronal Ceroid Lipofuscinosis (JNCL), a form of Batten disease that is grouped within the broad class of lysosomal storage diseases. JNCL displays a primary central nervous system phenotype characterized by rapid onset blindness, wide spread brain atrophy and reversal of learned abilities with death occurring 10-20 years after symptom onset. The mechanisms underlying these phenotypes are not known. CLN3 encodes CLN3, a protein with no known molecular function. CLN3 is expressed at very low levels natively in most cells, and is highly hydrophobic.

Similar to other lysosomal storage diseases, it is difficult to ...


The Molecular Evolution Of Reproduction In Animals: Insights From Sexual And Asexual Rotifers, Sara Jeanette Hanson Dec 2013

The Molecular Evolution Of Reproduction In Animals: Insights From Sexual And Asexual Rotifers, Sara Jeanette Hanson

Theses and Dissertations

Sex and meiosis are ubiquitous in eukaryotes as the primary mode of reproduction. This suggests that despite the theoretical energetic advantages of asexual reproduction, organisms capable of sexual reproduction are at a much greater long-term evolutionary advantage. Rotifers, a group of microinvertebrates, offer unique opportunities to examine the evolution of sex due to their extensive proliferation, successful adaptation to a wide variety of ecological niches, and the diversity of reproductive modes represented in the group. The cyclically parthenogenetic monogonont rotifers have overcome constraints on the loss of sexual reproduction in order to frequently transition between sexual and asexual generations, making ...


Population Pharmacokinetics And Exposure Toxicity Analysis Of Vatalanib In Patients With Myelodysplastic Syndromes, Xiaofeng Wang Dec 2013

Population Pharmacokinetics And Exposure Toxicity Analysis Of Vatalanib In Patients With Myelodysplastic Syndromes, Xiaofeng Wang

Theses and Dissertations

Vatalanib is a novel, potent, orally bioavailable angiogenesis inhibitor which can block all known vascular endothelial growth factor (VEGF) receptor tyrosine kinases. It has been shown to have potential in treating hematological malignancies, including myelodysplastic syndromes (MDS). Previous studies have revealed substantial pharmacokinetic variability that is further increased by auto-induction of its metabolism. And safety and tolerability are of great importance for such anti-angiogenic treatment. Therefore, the objectives of thesis was to characterize the pharmacokinetics and auto-induction process of vatalanib using population pharmacokinetic approaches, and to evaluate the relationship between systemic exposure to vatalanib and its toxicity in order to ...


Effects Of Three Deafness-Causing Gamma-Actin Mutations On Actin Structure And Function, Karina Annette Kruth Dec 2013

Effects Of Three Deafness-Causing Gamma-Actin Mutations On Actin Structure And Function, Karina Annette Kruth

Theses and Dissertations

Hearing requires proper function of the auditory hair cell, which is critically dependent upon its actin-based cytoskeletal structure. Eleven point mutations in gamma (γ) nonmuscle actin have been identified as causing progressive autosomal dominant nonsyndromic hearing loss (DFNA20/26); however, exactly why these mutations lead to deafness is unclear. Organization, stability, and repair of the hair cell cytoskeleton are highly regulated by actin binding proteins (ABPs), and two of the mutations, K118M and K118N, are located near an area of the actin monomer believed to be important in actin-ABP interactions. A third mutation, D51N, is located in a region of ...


Applying Human Computation Methods To Information Science, Christopher Glenn Harris Dec 2013

Applying Human Computation Methods To Information Science, Christopher Glenn Harris

Theses and Dissertations

Human Computation methods such as crowdsourcing and games with a purpose (GWAP) have each recently drawn considerable attention for their ability to synergize the strengths of people and technology to accomplish tasks that are challenging for either to do well alone. Despite this increased attention, much of this transformation has been focused on a few selected areas of information science.

This thesis contributes to the field of human computation as it applies to areas of information science, particularly information retrieval (IR). We begin by discussing the merits and limitations of applying crowdsourcing and game-based approaches to information science. We then ...


Sepp1 And Foxm1 Regulate Oxidative Stress-Mediated Radiation Response, Jaimee Claire Eckers Dec 2013

Sepp1 And Foxm1 Regulate Oxidative Stress-Mediated Radiation Response, Jaimee Claire Eckers

Theses and Dissertations

Radiation is a common mode of cancer therapy that is well known to generate reactive oxygen species leading to cell damage and death. However, there are many limitations to radiation therapy including normal tissue toxicity and the presence of quiescent cancer cells that are radio-resistant. There are many factors that regulate normal and cancer cell radiation response including the cellular redox environment which includes a complex network of antioxidants. In this study, two specific objectives will be explored: (A) SEPP1 regulation of normal cell toxicity; and (B) FoxM1 regulation of quiescence-associated radiation resistance in human oral squamous carcinoma cells. Results ...


Defining The Interaction Of Esxa And Lcrf With Type Iii Secretion System Gene Promoters, Jessica Marie King Dec 2013

Defining The Interaction Of Esxa And Lcrf With Type Iii Secretion System Gene Promoters, Jessica Marie King

Theses and Dissertations

Transcription of the Pseudomonas aeruginosa type III secretion system is controlled by ExsA, a member of the AraC/XylS family of regulators. ExsA is comprised of an amino terminal domain that is involved in self-association and regulatory functions, and a carboxy-terminal domain that contains two helix-turn helix (HTH) DNA-binding motifs which contact promoter DNA. Previous work from our lab determined the function of the two independent ExsA domains and found that each ExsA-dependent promoter contains two adjacent binding sites for monomeric ExsA. The promoter-proximal site (binding site 1) consists of highly conserved GnC and TGnnA sequences that are individually recognized ...


Pharmacological Dissection Of The Actions Of The Mu Opioid Receptor In The Rostroventral Medial Medulla, Marlene Cano Dec 2013

Pharmacological Dissection Of The Actions Of The Mu Opioid Receptor In The Rostroventral Medial Medulla, Marlene Cano

Theses and Dissertations

Chronic pain is a significant healthcare problem. It is disabling and diminishes quality of life. Opioids, such as morphine, remain a primary pharmacologic management for chronic pain. Opioids act at mu opioid receptors (MOPr) in the rostroventral medial medulla (RVM) to produce their analgesic effect. The RVM is a critical relay in pain inhibitory and facilitatory pathways of pain modulation. Furthermore, chronic inflammatory pain, produced by CFA hindpaw injection, leads to adaptive changes in the RVM that change the balance of these pathways and increase the potency of opioids.

MOPr are known to produce their effects via Gi/o proteins ...


Novel Roles For Y-Protocadherins In The Choroid Plexus, Mark Albert Lobas Dec 2013

Novel Roles For Y-Protocadherins In The Choroid Plexus, Mark Albert Lobas

Theses and Dissertations

Γ-protocadherins (Γ-Pcdhs) are important for neuronal development and regular nervous system patterning. Much of this work is based on the assumption that this family of 22 cadherin-like adhesion molecules acts in the manner of Roger Sperry's hypothesized "molecular code", with homophilic adhesion allowing neurons to find their proper neuronal partners during development. Therefore, most research has focused on the expression and roles of these adhesion molecules in neurons and glia. Although these molecules have been almost exclusively studied in neurons, there is evidence that Γ-Pcdhs are also expressed and play important roles in other cells. The work done for ...


Microrna Regulation Of Central Nervous System Development And Their Species-Specific Role In Evolution, Hayley Sarah Mcloughlin Dec 2013

Microrna Regulation Of Central Nervous System Development And Their Species-Specific Role In Evolution, Hayley Sarah Mcloughlin

Theses and Dissertations

Genetic dissection of loci important in the control of neurogenesis has improved our understanding of both the evolutionarily conserved and divergent processes in neurodevelopment. These loci include not only protein coding genes [1, 2], but also noncoding RNAs [3-5]. One important family of non-coding RNAs is miRNAs, which control gene expression fundamental in developmental regulation and mature cell maintenance [3, 5-9].

Here, we will first focus our efforts by surveying miRNA regulation in the developing brain. We hypothesize a strong regulatory role of miRNAs during proliferation, cell death, migration and differentiation in the developing mammalian forebrain that has yet to ...


The Molecular Mechanisms Of Pitx2 In Tooth Development And Enamel Defects In Axenfeld-Rieger Syndrome, Xiao Li Dec 2013

The Molecular Mechanisms Of Pitx2 In Tooth Development And Enamel Defects In Axenfeld-Rieger Syndrome, Xiao Li

Theses and Dissertations

Patients with Axenfeld-Rieger Syndrome (ARS) present various dental abnormalities. ARS is genetically associated with mutations in the PITX2 gene, which encodes one of the earliest transcription factors to initiate tooth development. Thus, Pitx2 has long been considered as an upstream regulator of the transcriptional hierarchy in tooth development. However, it is unclear how its mutant forms cause ARS dental anomalies. In this report, we outline the transcriptional mechanism that is defective in ARS. We demonstrate that during normal tooth development Pitx2 activates Amelogenin (Amel) expression, whose product is required for enamel formation, and that this regulation is perturbed by missense ...


A Forward Genetics Approach To Identify Molecular Drivers Of Liver Cancer Using Sleeping Beauty Mouse Models, Jesse Daniel Riordan Dec 2013

A Forward Genetics Approach To Identify Molecular Drivers Of Liver Cancer Using Sleeping Beauty Mouse Models, Jesse Daniel Riordan

Theses and Dissertations

Each year liver cancer kills more than half a million people, making it the third

leading cause of cancer-related death worldwide. Annual incidence continues to rise steadily, both domestically and globally, increasing the burden of this disease. Advancements in the ability to obtain detailed molecular profiles of tumors have led to the successful development of targeted therapies for a number of different cancers. Unfortunately, however, the molecular pathogenesis of liver cancer is poorly understood relative to many other types of malignancies. Thus, the identification of factors contributing to the development and progression of liver tumors is a major goal of ...


Genetic Determinants Of Metabolic Syndrome In Lyon Hypertensive Rats, Man Chun John Ma Dec 2013

Genetic Determinants Of Metabolic Syndrome In Lyon Hypertensive Rats, Man Chun John Ma

Theses and Dissertations

Metabolic Syndrome (MetS) is a collective term for a cluster of disorders, including dysglycemia, central obesity, dyslipidemia, hypertension, and eventual end organ damage. The combination of these disorders increases the risk of many kinds of end organ damages, including coronary heart disease, kidney failure, and cirrhosis. MetS is highly prevalent in the United States, affecting one third of the U.S. population in a 2009 estimate.

The Lyon strains are three rat strains selectively inbred from the same colony of outbred rats for different blood pressure levels. The Lyon Hypertensive (LH) strain, in addition to its essential hypertension phenotype, also ...


A Prickly Situation: Prickle1 Function Depends On The Signaling Context, Tian Yang Dec 2013

A Prickly Situation: Prickle1 Function Depends On The Signaling Context, Tian Yang

Theses and Dissertations

The gene PRICKLE1 is important for human brain function, as mutations in PRCKLE1 are associated with progressive myoclonus epilepsy (PME). Mutations in prickle orthologs could cause seizures in flies, zebrafish and mice, suggesting a conserved role of Prickle protein in seizure from fruit flies to humans. The underlying molecular mechanism how PRICKLE1 mutation causes PME is still unknown.

Prickle1 is part of the planar cell polarity (PCP) pathway, which regulates cell polarity within plane of cell sheets. In Drosophila, prickle is recruited to one side of the cell by another PCP protein, Van Gogh. This asymmetric protein localization of Van ...


Generation And Characteriztion Of Regulatory Dendritic Cells For The Amelioration Of Acute Graft Versus Host Disease, Sabrina Marie Scroggins Dec 2013

Generation And Characteriztion Of Regulatory Dendritic Cells For The Amelioration Of Acute Graft Versus Host Disease, Sabrina Marie Scroggins

Theses and Dissertations

Despite Human Leukocyte Antigen (HLA) matching and use of immunosuppressive drugs, graft-versus-host disease (GVHD) following hematopoietic stem cell transplant (HSCT) is prevalent and often fatal. Additionally, older HSCT recipients experience increased morbidity and mortality. Prophylactic treatment with age-matched syngeneic (recipient strain-derived) cultured regulatory DC (DCreg) has been shown to decrease GVHD-associated mortality in young bone marrow transplanted (BMT) mice. The purpose of this study was to investigate: 1) the potential to generate DCreg from older mice and their subsequent ability to ameliorate GVHD in older BMT mice, 2) the mechanism(s) by which DCreg mitigate GVHD in vivo, 3) the ...


The Related Kinases Fak And Pyk2 Serve Distinct Functions In Tcr-Mediated T Cell Activation, Nicole Chapman Dec 2013

The Related Kinases Fak And Pyk2 Serve Distinct Functions In Tcr-Mediated T Cell Activation, Nicole Chapman

Theses and Dissertations

T cells are central regulators of adaptive immunity in infectious and pathophysiological diseases. The activation of T cells is regulated by the T cell antigen receptor (TCR) and co-stimulatory receptors like toll-like receptor 2 (TLR2). These receptors activate distinct and overlapping intracellular signaling pathways that ultimately shape T cell responses. Therefore, studies that elucidate the molecular mechanisms of signal transduction downstream of receptors like the TCR and TLR2 will highlight key pathways required for T cell activation. These pathways could then be clinically targeted to alter dysfunctional T cell responses that promote many human diseases.

Focal adhesion kinase (FAK) and ...


Regulation Of Cgrp Gene Expression And Effects On Light Aversive Behavior, Ann Christine Raddant Dec 2013

Regulation Of Cgrp Gene Expression And Effects On Light Aversive Behavior, Ann Christine Raddant

Theses and Dissertations

Migraine is a debilitating neurological disorder, which affects over 10% of the general population. In addition to headache, migraine includes a host of associated symptoms, such as nausea and hypersensitivity to light, noise, and touch. While great strides have been made in migraine treatment in recent decades, the basic biological and pathophysiological mechanisms underlying migraine are still not well understood. Pain signals travel via a polysynaptic pathway from the periphery to the cortex, where conscious perception of pain occurs. This multi-neuron pathway produces a message that can be modified at any step of its transit.

One peptide that may modify ...


Manganoporphyrins As Adjuvants To Enhance Pharmacological Ascorbate In Pancreatic Cancer Therapy, Malvika Rawal Dec 2013

Manganoporphyrins As Adjuvants To Enhance Pharmacological Ascorbate In Pancreatic Cancer Therapy, Malvika Rawal

Theses and Dissertations

With new insights on mechanism, there is renewed interest in the use of pharmacological ascorbate (AscH-) in cancer therapy. The generation of H2O2 with AscH- acting as an electron donor to O2 is central to AscH- -induced cytotoxicity. We hypothesized that catalytic manganoporphyrins (MnPs) would increase the rate of oxidation of AscH- thereby increasing the flux of H2O2, resulting in increased cytotoxicity. We tested three different MnPs: MnTBAP, MnT2EPyP, and MnT4MPyP, which represent a range of physicochemical and thermodynamic properties. Of the MnPs tested, MnT4MPyP had the greatest effect on increasing the rate of oxidation of AscH-, as seen by ...


Endothelial Agonists Stimulate Vwf Release In Vitro And Trigger Ttp In Vivo, Gilbert Van Schaeffer Dec 2013

Endothelial Agonists Stimulate Vwf Release In Vitro And Trigger Ttp In Vivo, Gilbert Van Schaeffer

Theses and Dissertations

Von Willebrand factor (VWF) is a plasma glycoprotein that can bind collagen at a wound site as well as circulating platelets. VWF forms high molecular weight multimers (>20,000 kDa). VWF can also form VWF strings that appear to be attached to the endothelial surface and are capable of binding platelets. These strings are only observed in vitro and in vivo in the absence of the VWF-cleaving protease ADAMTS13. Deficiency in ADAMTS13 results in thrombotic thrombocytopenic purpura (TTP), a clotting disorder characterized by thrombocytopenia, microangiopathic hemolytic anemia, renal dysfunction, neurological dysfunction and fever. Patients suffering from TTP demonstrate VWF-and platelet-rich ...


Dystroglycan In Cerebellar Development And Disease, Huy Tuan Nguyen Dec 2013

Dystroglycan In Cerebellar Development And Disease, Huy Tuan Nguyen

Theses and Dissertations

Dystroglycanopathies are muscular dystrophies caused by mutations in genes involved the in O-linked glycosylation of alpha-dystroglycan. Severe forms exhibit brain and ocular developmental abnormalities in addition to muscular dystrophy. While cerebellar dysplasia is a common feature of dystroglycanopathy, its pathogenesis has not been thoroughly investigated. Here we evaluate the role of dystroglycan during cerebellar development. Brain-selective deletion of dystroglycan does not affect overall cerebellar growth, but causes malformations associated with glia limitans disruptions and granule cell heterotopia that recapitulate phenotypes found in dystroglycanopathy patients. Cerebellar pathology in these mice is not evident until birth even though dystroglycan is lost during ...


The Evolution And Expression Of Drosophila Meiosis Genes, Danielle Jeanine Beekman Dec 2013

The Evolution And Expression Of Drosophila Meiosis Genes, Danielle Jeanine Beekman

Theses and Dissertations

Drosophila melanogaster is unique amongst model organisms in that males utilize achiasmatic meiosis, where formation of the synaptonemal complex (SC) and recombination are absent. Most organisms require the SC and chiasmata for the successful completion of meiosis and production of viable gametes, making D. melanogaster an ideal system for the study of meiotic variation. The goal of my research was to examine in detail the origin and evolution of male achiasmatic meiosis in Diptera. This was done in three parts: 1) assessing the presence and absence of meiosis genes across dipteran species, 2) analyzing the rate of evolution of Drosophila ...


The Two-Component System, Arlrs, Regulates Agglutination And Pathogenesis In Staphylococcus Aureus, Jennifer Nicole Walker Jul 2013

The Two-Component System, Arlrs, Regulates Agglutination And Pathogenesis In Staphylococcus Aureus, Jennifer Nicole Walker

Theses and Dissertations

Staphylococcus aureus is defined by its ability to agglutinate during exposure to human blood plasma. Although agglutination has long correlated with disease severity, the function of agglutination during infection remains unclear. Increasing evidence suggests the mechanisms of agglutination are highly complex and poorly understood. The goal of this dissertation was to characterize the mechanisms required for S. aureus agglutination in vitro and determine how these factors contribute to pathogenesis.

Chapter II focuses on the development of two in vitro agglutination assays, which allow the process to be measured quantitatively. Through these assays, we confirmed the major factors contributing to agglutination ...


Regulation And Function Of Staphylococcus Aureus Secreted Proteases On Biofilm Integrity, Joe Michael Mootz Jul 2013

Regulation And Function Of Staphylococcus Aureus Secreted Proteases On Biofilm Integrity, Joe Michael Mootz

Theses and Dissertations

Staphylococcus aureus is a known cause of chronic biofilm infections. Recent studies have demonstrated the importance of proteinaceous material in the biofilm matrix. S. aureus secretes at least ten proteases and there is growing evidence that these enzymes have self-cleavage roles that alter biofilm integrity. The goal of this dissertation is to characterize the function of secreted proteases with respect to S. aureus biofilms. In addition, we aimed to determine whether a known regulator of S. aureus proteases, the Regulator of toxins (Rot), is also a regulator of biofilms.

Studies presented in Chapter II utilize a mutation in Sigma factor ...


Production Of And Response To The Cannibalism Peptide Sdp In Bacillus Subtilis, Tiara G. Perez Morales Jul 2013

Production Of And Response To The Cannibalism Peptide Sdp In Bacillus Subtilis, Tiara G. Perez Morales

Theses and Dissertations

The Gram positive soil dwelling bacteria Bacillus subtilis produces spores when encountered with a low nutrient environment. However, B. subtilis can delay spore production by a mechanism known as cannibalism. Cannibalism is a process by which B. subtilis delays commitment to sporulation by killing a subpopulation of its cells. This process involves production of two toxins, SDP and SKF. SDP is a 42 amino acid peptide with a disulfide bond derived from the internal cleavage of its precursor protein pro-SdpC. pro-SdpC is part of the sdpABC operon. Production of extracellular SDP induces expression of the sdpRI operon. Encoded in this ...