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Full-Text Articles in Life Sciences

Effects Of Ergot Alkaloids And Bovine Bodily Fluids On Cytochrome P450 Activity, Nicholas Scott Ezell May 2013

Effects Of Ergot Alkaloids And Bovine Bodily Fluids On Cytochrome P450 Activity, Nicholas Scott Ezell

Theses and Dissertations

This thesis evaluates the PromegaTM P450-Glo assay (PromegaTM V9800) as a tool for quantifying ergot alkaloid concentration. Current techniques used for detection of ergot alkaloids are slow and expensive, do not detect all ergot alkaloids, or are not effective on bovine bodily fluids. The first study was conducted to determine effects of commercial ergot alkaloids (n = 6; 0 - 400 μM) on the PromegaTM P450-Glo assay. Cytochrome P450 (CYP450) activity in assay had a differential response to each ergot alkaloid and concentration. As concentrations of ergotamine, dihydroergotamine, ergocornine, and ergocryptine increased CYP450 activity was inhibited (P < 0.05). Increases in ergonovine and pergolide concentration did not affect (P > 0.1 ...


Relationship Of Lactate Dehydrogenase & Prolactin Genotypes To Immune Function, Growth, And Composition Of Replacement Heifers Grazing Tall Fescue, Christina Marie Turner May 2013

Relationship Of Lactate Dehydrogenase & Prolactin Genotypes To Immune Function, Growth, And Composition Of Replacement Heifers Grazing Tall Fescue, Christina Marie Turner

Theses and Dissertations

Objectives of this research were to determine effects of LDH enzymatic activity and circulating PRL on immune response, growth, composition, and reproduction, and to determine the effects of single nucleotide polymorphisms of LDH and PRL on immune function, growth and carcass traits, cyclicty, and fecundity of replacement heifers grazing endophyte infected tall fescue. Eighty-nine Angus based heifers had physical and ultrasound measurements along with blood samples collected at weaning, yearling, and prebreeding. Estrus detection was conducted for 10 d post each collection period, except prebreeding. Pregnancy status was verified after AI and again after bull removal. Heifers were maintained on ...