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Full-Text Articles in Life Sciences
Formation, Functionalization, Characterization, And Applications Of A Mixed-Mode, Carbon/Diamond-Based, Core-Shell Phase For High Performance Liquid Chromatography, Landon A. Wiest
Theses and Dissertations
My work has focused on a variety of different types of diamond-based, core-shell particles. These particles are formed with inert cores and poly(allylamine)/nanodiamond shells. Their intended purpose is to form an LC stationary phase that is stable from pH 1 – 14 and at elevated temperatures. At the beginning of my studies, the particles that had been made in the Linford laboratory were pH stable, but irregular and had poor mechanical stability. Since that time, I have worked to improve the particles by using more spherical zirconia and carbon cores, and I have improved their mechanical stability via chemical ...
Investigations Of Bioactivity, Disposition, And Metabolism Of Lipids Through Liquid Chromatography-Mass Spectrometry, Nathaniel W. Snyder
Publicly Accessible Penn Dissertations
Inflammatory diseases and multiple human cancers are associated with increased cyclooxygenase-2 (COX-2) expression together with decreased expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH). This has been hypothesized to lead to a milieu of bioactive lipids modulating angiogenesis and cellular proliferation. Further insight into these processes were gained by traditional cell and molecular biology approaches, which were combined with cutting edge liquid chromatography mass spectrometry (LC-MS) methods to elucidate the function, disposition, and metabolism of a major COX-2/15-PGDH derived metabolite, 11-oxo-eicosatetraenoic acid (11-oxo-ETE). 11-oxo-ETE was found to possess significant anti-proliferative activity (IC50 2 microM) in human umbilical endothelial cells (HUVECS), which was ...