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Theses/Dissertations

2013

Chemistry

Amide ligation

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Full-Text Articles in Life Sciences

N-Terminal Protein Modification By Aminoacyl Transferase: Enzymology And Applications., Anne Wagner Jan 2013

N-Terminal Protein Modification By Aminoacyl Transferase: Enzymology And Applications., Anne Wagner

Publicly Accessible Penn Dissertations

Protein modification is an important tool for understanding how a protein moves, folds, and operates in its cellular environment. We aim for a minimalist approach to protein modification in order to learn more about the protein while not perturbing its native state. Our method for ligation of small molecules to proteins has high N-terminal specificity and creates natural amide bond linkages under mild conditions. We utilized E. coli aminoacyl L/F transferase (AaT) in our ligation reaction to recognize a small synthetic substrate analog, which mimics aminoacylated tRNA, and can be chemically synthesized in only three steps. We have studied ...