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Theses/Dissertations

2013

Chemistry

Electronic Thesis Collection

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The Cloning And Expression Of Histone Genes From The Archaeal Organisms Pyrococcus Furiosus And Methanobrevibacter Smithii In The Polycistronic Vector Pst44., Doha Abdullah Alqurashi Jan 2013

The Cloning And Expression Of Histone Genes From The Archaeal Organisms Pyrococcus Furiosus And Methanobrevibacter Smithii In The Polycistronic Vector Pst44., Doha Abdullah Alqurashi

Electronic Thesis Collection

The Euryarcheota branch of the Archaea contains histone proteins that are highly homologous to eukaryotic histones. Virtually all species within this group have two histone proteins (designated H1A and H2B). These proteins contain a canonical histone fold motif that stabilizes homo- and perhaps hetero-dimeric interactions. Molecular modeling calculations previously carried out in our laboratory revealed that both homo and hetero-dimers of histones from two euryarchaeal organisms are equally plausible. We have also shown through molecular modeling that inter-species hetero-dimers are structurally identical to existing solution structures archaeal histones. An interesting question that needs to be addressed is whether homo- and ...