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Full-Text Articles in Life Sciences

Shroom3 Deficient Mice Show Congenital Heart Defects, Rami R. Halabi Aug 2013

Shroom3 Deficient Mice Show Congenital Heart Defects, Rami R. Halabi

Electronic Thesis and Dissertation Repository

Congenital heart defects (CHDs) are associated with a number of genetic and environmental risk factors affecting approximately 1% of newborns. Shroom3 is an actin binding and microtubule organizing protein essential for neural tube closure in mouse, Xenopus and chick. In Xenopus shroom3 expression is found within the forming heart and loss of activity results in malformed hearts. In addition, SHROOM3 has recently been associated with heterotaxy in a human patient. Mice homozygous for the Shroom3 gene trap die at birth due to exencephaly and here, I provide evidence that the majority of these mice have CHDs, including septal defects, semilunar ...


Identification Of Actin Cytoskeletal Protein Interactions With The Fission Yeast Scaffold, Mid1, Marian Testori Aug 2013

Identification Of Actin Cytoskeletal Protein Interactions With The Fission Yeast Scaffold, Mid1, Marian Testori

Masters Theses

Cancer is a disease of uncontrolled cell division. Cell division in eukaryotic cells occurs by the formation of a contractile ring, predominantly composed of filamentous actin (F-actin). During mitosis, actin filaments polymerize to form rings at the medial plane of the cell that constrict causing the cell membrane to pinch and divide. Proteins that regulate this process are essential because dysregulation can lead to uninhibited cell growth and division. In fission yeast, the protein Mid1 functions as a scaffold to recruit regulatory proteins required for actin filament formation and simultaneously anchors the contractile ring at the cell division site. The ...


Susceptibility Of Apoptotic Cells To Hydrolysis By Spla2: Molecular Basis And Mechanisms Defined, Elizabeth Gibbons Jul 2013

Susceptibility Of Apoptotic Cells To Hydrolysis By Spla2: Molecular Basis And Mechanisms Defined, Elizabeth Gibbons

Theses and Dissertations

Secretory phospholipase A2 hydrolyzes phospholipids at a lipid-water interface, resulting in pro-inflammatory products being released from cell membranes. Healthy cells are resistant to cleavage by this enzyme, but apoptotic cells become susceptible to its activity. Only bilayers with certain characteristics are able to be hydrolyzed. Most recently, studies in this lab have emphasized the idea that the biophysical state of the bilayer (in terms of lipid order, spacing, and fluidity) is relevant in determining the probability of one phospholipid escaping the membrane to be hydrolyzed. Prior to this study, it had been shown that apoptotic cells undergo biophysical alterations that ...


Subcellular Analysis Of The Disulfide Proteome In P66shc Expressing Nerve Cells, Tyler Cann Jan 2013

Subcellular Analysis Of The Disulfide Proteome In P66shc Expressing Nerve Cells, Tyler Cann

Electronic Thesis and Dissertation Repository

The longevity associated protein p66Shc has been suggested to regulate organismal lifespan through initiation of apoptotic pathways. Following stress-induced translocation into the mitochondria, p66Shc promotes increased reactive oxygen species (ROS) production and triggers poorly defined downstream signaling events that lead to decreased cell viability. Protein disulfide bonding has recently emerged as aROSdependent post-translational modification that regulates protein function and signaling processes. Using the mouse hippocampal HT-22 cell line, I sought to determine the changes in the disulfide proteome associated with p66Shc mediatedROSproduction. Through Redox 2D-SDSPAGEanalysis of mitochondrial and cytosolic extracts, redox sensitive proteins altered by p66Shc mediatedROSformation were identified. Of ...