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Theses/Dissertations

2013

Cell and Developmental Biology

Honors Projects and Presentations: Undergraduate

Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

Methanothermus Fervidus Histone Binding Affinity And Packaging Of Archaeal Dna, Natalie Ziegler, Hannah S. Tims Jan 2013

Methanothermus Fervidus Histone Binding Affinity And Packaging Of Archaeal Dna, Natalie Ziegler, Hannah S. Tims

Honors Projects and Presentations: Undergraduate

Each domain of life requires specific strategies for compacting the large amount of genomic material within the spatial confines of the cell. Packaging of the DNA is essential for protection, genetic stability, and gene accessibility. Archaea are categorized as prokaryotes and thrive under diverse environmental conditions; however they use a mechanism for DNA packaging similar to eukaryotes. Eukaryotes and archaea compact their DNA in nucleosome structures by wrapping their DNA around complexes of histone proteins. Although similarities exist between histone structure in eukaryotes and archaea, it is unknown how the proteins and DNA are stabilized under diverse conditions under which ...


Construction Of A Novel System To Analyze Recognition Of Mhc Class Ii-Restricted Epitopes By Cd4+ T Cell Hybridoma Clones In Vitro, Samuel Bray, Lawrence Mylin Jan 2013

Construction Of A Novel System To Analyze Recognition Of Mhc Class Ii-Restricted Epitopes By Cd4+ T Cell Hybridoma Clones In Vitro, Samuel Bray, Lawrence Mylin

Honors Projects and Presentations: Undergraduate

The Simian Virus 40 large tumor antigen (SV40 Tag) is an excellent model system for studies in cancer immunology because it is strongly immunogenic, but capable of causing tumors when expressed as a transgene in vivo when protected by immune tolerance. The CD8+ T cell response to Tag in the C57BL/6 murine system is directed against four well-characterized epitopes. However, the CD4+ T cell response in this system remains uncharacterized. Recent work using Tag-specific CD4+ hybridoma clones and immune splenocytes to screen a 175 member, overlapping 15-mer peptide library corresponding to the 708 amino acid Tag protein sequence have ...


Expression Of The Human Gastrin Receptors Cckbr And Cckcr In Panco2 Murine Pancreatic Cancer Cells, Shannon V. Sell, Glenn R. Jones, Gail L. Matters, John F. Harms Jan 2013

Expression Of The Human Gastrin Receptors Cckbr And Cckcr In Panco2 Murine Pancreatic Cancer Cells, Shannon V. Sell, Glenn R. Jones, Gail L. Matters, John F. Harms

Honors Projects and Presentations: Undergraduate

Pancreatic cancer is the fourth leading cause of cancer mortality. The gastrointestinal hormone, gastrin, stimulates human pancreatic cancer growth via its receptor, CCKBR, and a splice-variant of CCKBR (termed CCKCR) detected only in cancer cells. This cancer-associated variant retains the fourth intron and exhibits increased cell signaling. Recently, a single nucleotide polymorphism (SNP; C>A) in the fourth intron was identified. Presence of SNP(A) was correlated with CCKCR protein expression in pancreatic cancer specimens and decreased patient survival. We hypothesize expression of human CCKCR in murine pancreatic cancer cells will increase tumor growth and metastasis relative to expression of ...