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Full-Text Articles in Life Sciences

Microbial Dynamics And Core Microbiome Of Red-Backed Salamanders (Plethodon Cinereus), Andrew Howard Loudon May 2013

Microbial Dynamics And Core Microbiome Of Red-Backed Salamanders (Plethodon Cinereus), Andrew Howard Loudon

Masters Theses, 2010-2019

Beneficial cutaneous bacteria on amphibians can protect against the lethal fungal disease chytridiomycosis, which has decimated many amphibian species. The stability of these bacterial communities likely influences health outcomes, and is investigated here for the first time. We describe the diversity of bacteria on red-backed salamanders (Plethodon cinereus) in the wild, and the stability of these communities over time in captivity using culture-independent Illumina sequencing. In the field, there was no correlation between the diversity of salamanders’ microbial communities and the diversity of their substrates’ microbial communities. Salamanders were brought into the laboratory to test for the effect of an ...


Probiotic Bioaugmentation Of An Anti-Bd Bacteria, Janthinobacterium Lividum, On The Amphibian, Notophthalmus Viridescens: Transmission Efficacy And Persistence Of The Probiotic On The Host And Non-Target Effects Of Probiotic Addition On Ecosystem Components, Molly Bletz May 2013

Probiotic Bioaugmentation Of An Anti-Bd Bacteria, Janthinobacterium Lividum, On The Amphibian, Notophthalmus Viridescens: Transmission Efficacy And Persistence Of The Probiotic On The Host And Non-Target Effects Of Probiotic Addition On Ecosystem Components, Molly Bletz

Masters Theses, 2010-2019

Amphibians worldwide are threatened by the fungal disease chytridiomycosis, caused by the skin pathogen Batrachochytrium dendrobatidis. Mutualistic skin bacteria are a critical element in amphibians’ defenses against chytridiomycosis. Probiotic bioaugmentation of beneficial, anti-Bd bacteria on amphibians is a potential conservation strategies. Outdoor experimental ponds were used to investigate transmission efficacy and persistence of the anti-Bd bacteria, Janthinobacterium lividum, on the amphibian, Notophthalmus viridescens. More specifically, this research investigated whether a short-term individual bath, environmental bioaugmentation, or both are necessary to afford transmission and persistence of J. lividum on N. viridescens. Additionally, this research investigated the effectiveness of these different probiotic ...


The Effects Of Chytridiomycosis On Myocardial Oxygenation In Columbian Spotted Frogs (Rana Luteiventris), Kaitlyn Holyfield Apr 2013

The Effects Of Chytridiomycosis On Myocardial Oxygenation In Columbian Spotted Frogs (Rana Luteiventris), Kaitlyn Holyfield

Life and Environmental Sciences Undergraduate Theses

Chytridiomycosis is an infection in amphibians caused by the fungus Batrachochytrium dendrobatidis that has been linked to world-wide amphibian decline. However, there is currently no clear explanation of how this infection is killing amphibians. I hypothesize that the fungus is preventing effective cutaneous gas exchange, causing hypoxia in the heart which could lead to myocardial ischemia. The objectives of this study are to find an organism that is susceptible to Chytridiomycosis which can be used as a model for detecting myocardial ischemia in frogs and to establish a protocol for ischemia detection. Rana luteiventris (Columbian Spotted Frogs) were collected and ...


Understanding Amphibian Decline: The Role Of Pesticides And The Pathogenic Chytrid Fungus On Amphibians And Aquatic Communities, Taegan A. Mcmahon Jan 2013

Understanding Amphibian Decline: The Role Of Pesticides And The Pathogenic Chytrid Fungus On Amphibians And Aquatic Communities, Taegan A. Mcmahon

Graduate Theses and Dissertations

Amphibians are the most threatened taxon on the planet. Declines have been associated with over-exploitation, habitat loss, pollution, and pathogenic diseases, but of these factors, pollution and disease have been relatively under-studied. Here, I investigated: 1) the impacts of commonly used pesticides on aquatic communities, 2) the effect of these pesticides on amphibian susceptibility to the pathogenic chytrid fungus, Batrachochytrium dendrobatidis (Bd), and 3) whether there are non-amphibian hosts of Bd and 4) how to best quantify the survival of Bd through ontogeny of the host.

In my first research chapter, I quantified the effects of environmentally relevant concentrations of ...