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Full-Text Articles in Life Sciences

A Novel Rcc1-Like Protein Is A Crucial Regulator Of The Intraerythrocytic Cycle Of The Human Malaria Parasite, Plasmodium Falciparum., Marcus Davon Skaflen Dec 2013

A Novel Rcc1-Like Protein Is A Crucial Regulator Of The Intraerythrocytic Cycle Of The Human Malaria Parasite, Plasmodium Falciparum., Marcus Davon Skaflen

Masters Theses

Malaria is a deadly infection caused by a single celled protozoan of the Plasmodium genus. Plasmodium spp. are transmitted to humans by mosquitoes, and initially invade the liver, but the disease is caused by the blood stage of the infection. Approximately 500 million cases of malaria are documented annually and over 1 million of those result in death. Plasmodium falciparum is the most lethal of five species known to infect humans. To further compound this problem, drug-resistant parasite strains have been documented for every currently available antimalarial drug, making the need to identify new drug targets more urgent than ever ...


Small Scale Population Structure Of Pityopsis Ruthii And P. Graminifolia Var. Latifolia, Sarah Lynn Boggess Dec 2013

Small Scale Population Structure Of Pityopsis Ruthii And P. Graminifolia Var. Latifolia, Sarah Lynn Boggess

Masters Theses

Pityopsis graminifolia var. latifolia (Michx.) Small var. latifolia (Fern.) or narrowleaf silkgrass is an herbaceous perennial native to the southeastern United States and northern Central America. Pityopsis ruthii (Small) Small is an endangered plant endemic to the Hiwassee and Ocoee Rivers in Polk County, Tennessee. Little is known about the genetic diversity and population structure of both Pityopsis species as well as the phylogenic relationships between the species. In this study, 16 microsatellite loci were used to assess genetic diversity and population structure of 261 individuals of P. ruthii (n=167) and P. graminifolia var. latifolia (n=76). Pityopsis ruthii ...


Climate Change And Metapopulation Implications For Species Re/Introductions: A Spatial Analysis Of Suitable Habitat For The American Marten (Martes Americana) In Northern Michigan, Joshua Michael Green Dec 2013

Climate Change And Metapopulation Implications For Species Re/Introductions: A Spatial Analysis Of Suitable Habitat For The American Marten (Martes Americana) In Northern Michigan, Joshua Michael Green

Masters Theses

The American marten (Martes americana), which was extirpated from Michigan by 1939 due to logging and trapping, has cultural significance as a clan animal to Great Lakes Native American Tribes and ecological significance as a forest health indicator. Sleeping Bear Dunes National Lakeshore (SBD) is considering reintroduction, but several factors must first be considered in assessing the habitat suitability. The goals of this study were to 1) enhance an existing habitat suitability model by including additional relevant variables, 2) conduct a spatial analysis of the habitat within the study area using a metapopulation perspective and 3) incorporate climate change predictions ...


Native Plant Establishment Success Influenced By Centaurea Stoebe Control Method, Laurelin Marie Martin Dec 2013

Native Plant Establishment Success Influenced By Centaurea Stoebe Control Method, Laurelin Marie Martin

Masters Theses

Invasive species frequently hinder restoration efforts. While the effectiveness of differing control methods are often reported, the impacts these methods have on the establishment of a native plant community are often unknown. To determine methods that effectively reduce spotted knapweed (Centaurea stoebe) while enhancing native species establishment, we tested 12 treatment combinations consisting of an initial site preparation (mowing, mowing + clopyralid, or mowing + glyphosate), with combinations of annual adult knapweed hand pulling, and/or burning. We established 48 plots and applied site preparation treatments during summer 2008, seeded 23 native forbs and grasses during spring 2009, pulled adult knapweed annually ...


Population Demographics And Genetic Structure Of Black Bears In Coastal Louisiana, Jesse Charles Troxler Dec 2013

Population Demographics And Genetic Structure Of Black Bears In Coastal Louisiana, Jesse Charles Troxler

Masters Theses

The range and abundance of the Louisiana black bear (Ursus americanus luteolus) were greatly diminished during the 20th century. This subspecies was reduced to 3 small, isolated subpopulations in Louisiana as bottomland hardwood habitat was converted to agriculture. These bears were listed as threatened by the U.S. Fish and Wildlife Service in 1992 and a recovery plan was published in 1995. Recovery requires estimates of population parameters to evaluate current population status and future viability. I conducted a mark-recapture study from 2010 to 2012 to estimate demographic parameters of the coastal population of Louisiana black bears. Because inbreeding is ...


Trends In Borrelia Spp. Prevalence In Ixodes Spp. Ticks From The Southeastern Coastal United States, Lauren Paul Maestas Aug 2013

Trends In Borrelia Spp. Prevalence In Ixodes Spp. Ticks From The Southeastern Coastal United States, Lauren Paul Maestas

Masters Theses

The Lyme borreliosis (LB) cycle, involving Ixodes scapularis and Borrelia burgdorferi sensu stricto (Bbss), is well documented in the northeastern US, where LB is becoming increasingly prevalent. In coastal North Carolina, I. affinis has been shown to have a higher incidence of Bbss than I. scapularis. My objectives were, to assess changes in prevalence of Bbss in Ixodes spp. along a transect from Virginia to Florida, and to assess the value of dogs and mesomammals as sentinels for spread of Borrelia burgdorferi sensu lato.

Ixodes spp. were collected at sites from 37.4o N to 30.0o N ...


Studies On Solo Working Mechanism In The Meiosis Of Drosophila Melanogaster, Qian Ma Aug 2013

Studies On Solo Working Mechanism In The Meiosis Of Drosophila Melanogaster, Qian Ma

Masters Theses

In eukaryotes, sister chromatids are closely aligned due to cohesion, a process essential for chromosome pairing and segregation during both mitosis and meiosis. A conserved cohesin complex in a ring structure is composed of four subunits, including each of these four members or their homologs, SMC1, SMC3, SCC1/RAD21/REC8, and SCC3/SA. Up to now, no REC8 homolog has been identified in the meiosis of Drosophila. SOLO is a meiotic protein required for accurate chromosome segregation, centromere cohesion, and cohesin complex localization in Drosophila meiosis. In addition, SOLO is required for synapsis and recombination in Drosophila female meiosis.

In ...


Prevalence And Transmission Potential Of Borrelia Burgdorferi In Three Species Of Wildcaught Plestiodon Spp. Skinks Of The Southeastern United States, Teresa Dianne Moody Aug 2013

Prevalence And Transmission Potential Of Borrelia Burgdorferi In Three Species Of Wildcaught Plestiodon Spp. Skinks Of The Southeastern United States, Teresa Dianne Moody

Masters Theses

In the southeastern United States, blue-tailed skinks (Plestiodon spp.) are important hosts for Ixodes scapularis ticks, the principal vector of Lyme disease (LD) in this region. Skinks and other southeastern lizards are not thought to be reservoir competent for Borrelia burgdorferi sensu stricto (Bbss), the etiological agent of LD in the United States. . Lizard-feeding by southeastern I. scapularis may tend to suppress sylvatic cycles of B. burgdorferi, and thus may be an important reason why LD case rates in the Southeast are much lower than in the Northeast and upper Midwest. Nevertheless, some skinks in Florida and South Carolina have ...


Probiotic Bioaugmentation Of An Anti-Bd Bacteria, Janthinobacterium Lividum, On The Amphibian, Notophthalmus Viridescens: Transmission Efficacy And Persistence Of The Probiotic On The Host And Non-Target Effects Of Probiotic Addition On Ecosystem Components, Molly Bletz May 2013

Probiotic Bioaugmentation Of An Anti-Bd Bacteria, Janthinobacterium Lividum, On The Amphibian, Notophthalmus Viridescens: Transmission Efficacy And Persistence Of The Probiotic On The Host And Non-Target Effects Of Probiotic Addition On Ecosystem Components, Molly Bletz

Masters Theses

Amphibians worldwide are threatened by the fungal disease chytridiomycosis, caused by the skin pathogen Batrachochytrium dendrobatidis. Mutualistic skin bacteria are a critical element in amphibians’ defenses against chytridiomycosis. Probiotic bioaugmentation of beneficial, anti-Bd bacteria on amphibians is a potential conservation strategies. Outdoor experimental ponds were used to investigate transmission efficacy and persistence of the anti-Bd bacteria, Janthinobacterium lividum, on the amphibian, Notophthalmus viridescens. More specifically, this research investigated whether a short-term individual bath, environmental bioaugmentation, or both are necessary to afford transmission and persistence of J. lividum on N. viridescens. Additionally, this research investigated the effectiveness of these different probiotic ...


Occurrence, Survival, And Persistence Of The Fecal Indicator Bacterium, Enterococcus Mundtii, In Soils Of A Contaminated Watershed, Marc Carpenter May 2013

Occurrence, Survival, And Persistence Of The Fecal Indicator Bacterium, Enterococcus Mundtii, In Soils Of A Contaminated Watershed, Marc Carpenter

Masters Theses

The Clean Water Act of 1972 raised awareness of the extent of the pollution in U.S. waters and the need for monitoring and identifying sources of contamination. Surface water quality is routinely evaluated using fecal indicators, such as Enterococcus spp. Recent studies in a south Texas watershed showed that fecal bacteria were being transported to water via agricultural land runoff following rainfall, suggesting that soil may be a source of these bacteria. In this study, soils from fields under different crop covers were sampled to determine seasonal levels of enterococci and species of enterococci were identified using the BIOLOG ...


The Potential For Replication And Transmission Of Antibiotic Resistance Plasmids In An E. Coli Population In Agriculturally Impacted Stream Sediment, Erika Gehr May 2013

The Potential For Replication And Transmission Of Antibiotic Resistance Plasmids In An E. Coli Population In Agriculturally Impacted Stream Sediment, Erika Gehr

Masters Theses

The use of antibiotics in agriculture is thought to be a major cause of resistance in microorganisms found in the environment. Horizontal transfer of genetic information from transient to native and from native to transient bacterial populations may enhance the spread and recombination of resistance genes and might play a role in the formation of multi-resistant organisms in environmental reservoirs. Tetracycline resistance plasmids were compared using three isolation techniques -- traditional “endogenous” extraction from isolates, “exogenous” plasmid capture, and direct plasmid extraction from sediment --to determine the potential for plasmid born resistance in an E. coli population found in agriculturally-impacted stream ...


Microbial Dynamics And Core Microbiome Of Red-Backed Salamanders (Plethodon Cinereus), Andrew Howard Loudon May 2013

Microbial Dynamics And Core Microbiome Of Red-Backed Salamanders (Plethodon Cinereus), Andrew Howard Loudon

Masters Theses

Beneficial cutaneous bacteria on amphibians can protect against the lethal fungal disease chytridiomycosis, which has decimated many amphibian species. The stability of these bacterial communities likely influences health outcomes, and is investigated here for the first time. We describe the diversity of bacteria on red-backed salamanders (Plethodon cinereus) in the wild, and the stability of these communities over time in captivity using culture-independent Illumina sequencing. In the field, there was no correlation between the diversity of salamanders’ microbial communities and the diversity of their substrates’ microbial communities. Salamanders were brought into the laboratory to test for the effect of an ...


Microarray Analysis Of Mrna Translation State In Arabidopsis Thaliana, Bayu Sisay Tiruneh May 2013

Microarray Analysis Of Mrna Translation State In Arabidopsis Thaliana, Bayu Sisay Tiruneh

Masters Theses

The synthesis of proteins – translation – is one of the cellular processes that consume a great deal of energy. Genome-wide assays performed in Arabidopsis and other organisms have revealed that the translation status of mRNAs responds dramatically to different stresses and environmental and growth signals. Similar assays have revealed how translation of specific mRNAs is perturbed by genetic mutations in eukaryotic translation initiation factors (eIFs). I performed genome-wide analyses of translation state in A. thaliana seedlings with mutant genotypes for a specific eIF (subunit h of eIF3) and the large ribosomal protein, L24 (RPL24B). Using data from prior microarray analysis of ...


Resolving The Nutritional Requirements Of The Bile Acid-Dehydroxylating Gut Bacterium Clostridium Scindens, Oindrila Paul Feb 2013

Resolving The Nutritional Requirements Of The Bile Acid-Dehydroxylating Gut Bacterium Clostridium Scindens, Oindrila Paul

Masters Theses

No abstract provided.


Parasites Of Invasive Carp And Native Fish In The Wabash River, Justin Wilcox Jan 2013

Parasites Of Invasive Carp And Native Fish In The Wabash River, Justin Wilcox

Masters Theses

No abstract provided.


Dietary Ecology Of An Actively-Foraging Snake Species, Coluber Constrictor Foxii, Corissa Pauline Lennon Jan 2013

Dietary Ecology Of An Actively-Foraging Snake Species, Coluber Constrictor Foxii, Corissa Pauline Lennon

Masters Theses

No abstract provided.


Freeze Tolerance Conferred By Transgenic Expression Of Late Embryogenesis Abundant Protein In Insect Cells, Alyssa N. Walser Jan 2013

Freeze Tolerance Conferred By Transgenic Expression Of Late Embryogenesis Abundant Protein In Insect Cells, Alyssa N. Walser

Masters Theses

No abstract provided.


Molecular Mechanisms Involved In Anhydrobiosis Of Insect Cells, Diyagama Arachchi Ralalage Dilini Sewwandi Samarajeewa Jan 2013

Molecular Mechanisms Involved In Anhydrobiosis Of Insect Cells, Diyagama Arachchi Ralalage Dilini Sewwandi Samarajeewa

Masters Theses

No abstract provided.


Using Field Studies To Meet The Next Generation Science Standards, Amy Brown Jan 2013

Using Field Studies To Meet The Next Generation Science Standards, Amy Brown

Masters Theses

No abstract provided.


Morphometric Study Of Variation In The Genus Galeopsis (Lamiaceae), Michael T. Hughes Jan 2013

Morphometric Study Of Variation In The Genus Galeopsis (Lamiaceae), Michael T. Hughes

Masters Theses

No abstract provided.


Cellular And Molecular Mechanisms Underlying The Interaction Of Apoe And Estrogen In Olfactory Neuronal Growth-Potential Relevance To Alzheimer's Disease, Minh Luong Jan 2013

Cellular And Molecular Mechanisms Underlying The Interaction Of Apoe And Estrogen In Olfactory Neuronal Growth-Potential Relevance To Alzheimer's Disease, Minh Luong

Masters Theses

No abstract provided.


Demographics Of A Commercially Exploited Population Of Flathead Catfish (Pylodictis Olivaris) In The Wabash River, Cassi Jo Moody-Carpenter Jan 2013

Demographics Of A Commercially Exploited Population Of Flathead Catfish (Pylodictis Olivaris) In The Wabash River, Cassi Jo Moody-Carpenter

Masters Theses

No abstract provided.


Short-Term Responses Of Fish Assemblages To Habitat Restoration In A Small Midwestern Stream, John Leon West Jan 2013

Short-Term Responses Of Fish Assemblages To Habitat Restoration In A Small Midwestern Stream, John Leon West

Masters Theses

No abstract provided.


Survey Of Medicinal Herbs Of Central Illinois Prairie And Woodlands, Debra Ann Welch Jan 2013

Survey Of Medicinal Herbs Of Central Illinois Prairie And Woodlands, Debra Ann Welch

Masters Theses

No abstract provided.


Wetting And Drying Cycles And The Fungal Communities On Leaf Litter In Streams, Kele Qwinn Thrailkill Jan 2013

Wetting And Drying Cycles And The Fungal Communities On Leaf Litter In Streams, Kele Qwinn Thrailkill

Masters Theses

"Leaf litter is a major source of energy for streams in deciduous forests. Fungi play a critical role by converting the leaves into nutritional material for the rest of the food web. The breakdown of leaf litter and associated biota, including invertebrates and fungi, have been proposed as measures of stream health in systems affected by anthropogenic activity. Rates of leaf breakdown can be depressed in streams affected by acidity, metals, organic contaminants, and other stressors. Climate change may lead to alterations in stream hydrology such that streams experience more frequent floods as well as drying episodes. Fungal communities can ...


Production Of 1,3-Propanediol From Glycerol Under Haloalkaline Conditions By Halanaerobium Hydrogeniformans, Daniel William Roush Jan 2013

Production Of 1,3-Propanediol From Glycerol Under Haloalkaline Conditions By Halanaerobium Hydrogeniformans, Daniel William Roush

Masters Theses

"With increased demands around the world to make modern lifestyles more environmentally friendly, the chemical commodity market has rapidly shifted. Through new technologies in chemical production, certain high value products have oversaturated the market and have become high-volume, low value waste products. The expansion of biodiesel production offers a prime example; high volumes of glycerol byproduct from this process have shifted glycerol from a high priced commodity to a common waste product. A number of microorganisms are known to synthesize the polymeric precursor 1,3-propanediol from glycerol; however, crude glycerol from biodiesel production creates a harsh environment for most microbes ...