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Characterization Of G-Patch Motif Contribution To Prp43 Function In The Pre-Messenger Rna Splicing And Ribosomal Rna Biogenesis Pathways, Daipayan Banerjee Jan 2013

Characterization Of G-Patch Motif Contribution To Prp43 Function In The Pre-Messenger Rna Splicing And Ribosomal Rna Biogenesis Pathways, Daipayan Banerjee

Theses and Dissertations--Biology

The DExD/H-box protein Prp43 is essential for two biological processes: nucleoplasmic pre-mRNA splicing and nucleolar rRNA maturation. The biological basis for the temporal and spatial regulation of Prp43 remains elusive. The Spp382/Ntr1, Sqs1/Pfa1 and Pxr1/Gno1 G-patch proteins bind to and activate the Prp43 DExD/H box-helicase in pre-mRNA splicing (Spp382) and rRNA processing (Sqs1, Pxr1). These Prp43-interacting proteins each contain the G-patch domain, a conserved sequence of ~48 amino acids that includes 6 highly conserved glycine (G) residues. Five annotated G-patch proteins in baker’s yeast (i.e., Spp382, Pxr1, Spp2, Sqs1 and Ylr271) and with ...


The Regulation And Packaging Of Synaptic Vesicles Related To Recruitment Within Crayfish And Fruit Fly Neuromuscular Junctions: Variations In Low- And High-Output Terminals, Wenhui Wu Jan 2013

The Regulation And Packaging Of Synaptic Vesicles Related To Recruitment Within Crayfish And Fruit Fly Neuromuscular Junctions: Variations In Low- And High-Output Terminals, Wenhui Wu

Theses and Dissertations--Biology

Glutamate is the main excitatory neurotransmitter in the CNS and at the neuromuscular junctions (NMJs) of invertebrate. The characteristic similarities to CNS glutamatergic synapses in vertebrate and the anatomical simplicity of invertebrate NMJs favor the investigation of glutamatergic synaptic functions in this system. This dissertation mainly aimed to physiologically separate two functional vesicle groups, the reserve pool (RP) and readily releasable pool (RRP) within presynaptic nerve terminals of Procambarus Clarkii and Drosophila melanogaster. This was addressed in part by blocking the vesicular glutamate transporter (VGlut) with bafilomycin A1. Various frequencies of motor nerve stimulation, exposure time, and concentration of bafilomycin ...


The Contribution Of Two Related Bbp-Binding Gyf Proteins, Smy2 And Syh1, To Cellular Rna Abundance And Genome Stability, Min Chen Jan 2013

The Contribution Of Two Related Bbp-Binding Gyf Proteins, Smy2 And Syh1, To Cellular Rna Abundance And Genome Stability, Min Chen

Theses and Dissertations--Biology

Nuclear precursor of mature messenger RNA (pre-mRNA) splicing is one of the most highly regulated processes in eukaryotic organisms. In addition to its role in the removal of constitutive or alternative introns present in the pre-mRNA, splicing is also highly integrated into other layers of gene expression. This study investigates the potential role of the nuclear branchpoint binding protein (BBP) outside of the pre-mRNA splicing cycle. More specifically, we were interested in the biological relevance of its association with two cytoplasmic proteins Smy2 and Syh1. Smy2 and Syh1 belong to the GYF family of poly-proline binding proteins, and their roles ...


Physical Dormancy In Seeds, With Special Reference To Geraniaceae: Morpho-Anatomy, Development, Physiology, Biomechanics And Classification Of Water-Gap Complexes, Nalin Suranjith Gama Arachchige Jan 2013

Physical Dormancy In Seeds, With Special Reference To Geraniaceae: Morpho-Anatomy, Development, Physiology, Biomechanics And Classification Of Water-Gap Complexes, Nalin Suranjith Gama Arachchige

Theses and Dissertations--Biology

The primary aims of this dissertation were to (1) identify and characterize the water-gap complex in seeds of Geraniaceae, (2) investigate its role in physical dormancy (PY) break and (3) develop a new classification system for water-gap complexes in seeds of angiosperms. The winter annuals Geranium carolinianum and G. dissectum were selected as the main representative species for the study, and seeds of an additional 29 species from the Geraniaceae were used to compare the water-gap complex within the family. A new classification system for water-gap complexes in species with PY was developed by comparing the morpho-anatomical features of PY ...


Investigation Of Media Ingredients And Water Sources For Algae Co2 Capture At Different Scales To Demonstrate The Correlations Between Lab-Scale And Large-Scale Growth, Tabitha Graham Jan 2013

Investigation Of Media Ingredients And Water Sources For Algae Co2 Capture At Different Scales To Demonstrate The Correlations Between Lab-Scale And Large-Scale Growth, Tabitha Graham

Theses and Dissertations--Biosystems and Agricultural Engineering

As energy use increases globally the environmental burdens increase alike. Many accusations have been made that carbon dioxide is a culprit of climate change. The University of Kentucky and Duke Energy Power have partnered to test carbon capture technology in a large scale project. To this end, the objective of this thesis is to investigate potential water media sources and nutrient sources at different volume scales for algae cultivation to help create a more environmentally viable and economically feasible solution. This work will conduct a life cycle assessment of water media sources and the effects of the inputs and outputs ...


Towards Elucidation Of A Viral Dna Packaging Motor, Chad T. Schwartz Jan 2013

Towards Elucidation Of A Viral Dna Packaging Motor, Chad T. Schwartz

Theses and Dissertations--Pharmacy

Previously, gp16, the ATPase protein of phi29 DNA packaging motor, was an enigma due to its tendency to form multiple oligomeric states. Recently we employed new methodologies to decipher both its stoichiometry and also the mechanism in which the protein functions to hydrolyze ATP and provide the driving force for DNA packaging. The oligomeric states were determined by biochemical and biophysical approaches. Contrary to many reported intriguing models of viral DNA packaging, it was found that phi29 DNA packaging motor permits the translocation of DNA unidirectionally and driven cooperatively by three rings of defined shape. The mechanism for the generation ...


Axolotl Paedomorphosis: A Comparison Of Juvenile, Metamorphic, And Paedomorphic Ambystoma Mexicanum Brain Gene Transcription, Carlena Johnson Jan 2013

Axolotl Paedomorphosis: A Comparison Of Juvenile, Metamorphic, And Paedomorphic Ambystoma Mexicanum Brain Gene Transcription, Carlena Johnson

Theses and Dissertations--Biology

Unlike many amphibians, the paedomorphic axolotl (Ambystoma mexicanum) rarely undergoes external morphological changes indicative of metamorphosis. However, internally, some axolotl tissues undergo cryptic metamorphic changes. A previous study examined interspecific patterns of larval brain gene expression and found that these species exhibited unique temporal expression patterns that were hypothesized to be morph specific. This thesis tested this hypothesis by examining differences in brain gene expression between juvenile (JUV), paedomorphic (PAED), and metamorphic (MET) axolotls. I identified 828 genes that were expressed differently between JUV, PAED, and MET. Expression estimates from JUV were compared to estimates from PAED and MET brains ...


Factors Influencing The Establishment And Survival Of Native Hardwood Tree Seedlings Of The Kentucky Inner Bluegrass Blue Ash-Oak Savanna-Woodland, James D. Shaffer Jan 2013

Factors Influencing The Establishment And Survival Of Native Hardwood Tree Seedlings Of The Kentucky Inner Bluegrass Blue Ash-Oak Savanna-Woodland, James D. Shaffer

Theses and Dissertations--Biology

Historically, the Kentucky Inner Bluegrass blue ash-oak savanna-woodland was the primary ecosystem of the Inner Bluegrass Region (IBR) of Kentucky. After European settlement, the majority (>99%) of Bluegrass savanna was converted to agricultural and urban land uses. Currently remnant savanna tree species are failing to recruit. Therefore, a long-term restoration ecology project researching competition and disturbance on seedling establishment, survival, and growth has been established at Griffith Woods (the largest remaining savanna in Kentucky) in Harrison Co., KY. Fourteen native hardwood tree species (a total of 6,168 seedlings) have been experimentally planted. Light, soil, surrounding vegetation, and herbivory, factors ...


Equine Serum Antibody Responses To Streptococcus Equi And Streptococcus Zooepidemicus, Rafaela De Negri Jan 2013

Equine Serum Antibody Responses To Streptococcus Equi And Streptococcus Zooepidemicus, Rafaela De Negri

Theses and Dissertations--Veterinary Science

Streptococcus zooepidemicus (Sz) and Streptococcus equi (Se) share 98% DNA sequence homology, but display different pathogenic properties. Infection by one organism does not cross-protect against the other. To better understand pathogenic differences between these organisms and gain information about which proteins are expressed in horses infected experimentally with Se, intrauterine Sz or naturally with respiratory Sz we compared antibody specificities of convalescent sera using ELISA. These comparisons were based on sets of 8 and 14 immunoreactive recombinant proteins of Se strain CF32 and Sz strain NC78, respectively. Sera from donkeys that were previously naturally affected with strangles and later developed ...


Effects Of Streamside Management Zone Timber Harvest On Salamander Communities In Robinson Forest, Thomas Maigret Jan 2013

Effects Of Streamside Management Zone Timber Harvest On Salamander Communities In Robinson Forest, Thomas Maigret

Theses and Dissertations--Forestry and Natural Resources

Salamanders are critical components of forest ecosystems, in terms of total biomass, as well as for their value as indicators of ecosystem stress. Considering the worldwide decline in amphibian populations, the known effects of timber harvest on salamander populations, and the importance of the forest products industry in Kentucky and elsewhere, the impacts of silvicultural operations on salamander communities cannot be overlooked. The objective was to investigate the effects of three different silvicultural treatments, each involving different streamside management zone (SMZ) characteristics, on salamander communities in ephemeral streams. Data were collected by regular checks of pitfall traps, coverboards, and transect ...