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Theses/Dissertations

2013

Biology

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Full-Text Articles in Life Sciences

The Regulation Of Prospore Membrane Shape In Saccharomyces Cerevisiae, Emily M. Parodi Dec 2013

The Regulation Of Prospore Membrane Shape In Saccharomyces Cerevisiae, Emily M. Parodi

Graduate Doctoral Dissertations

The size and shape of a membrane is an important determinant in cell morphology. My work focuses on how membrane size and shape is determined, using the budding yeast S. cerevisiae as a model. During sporulation in S. cerevisiae, the diploid cell remodels its interior such that four spores are formed within an ascus. During this remodeling process, the prospore membrane is synthesized de novo and grows to surround each of the meiotic products. The prospore membrane is a double lipid bilayer and ultimately determines the sizes and shapes of the newly formed spores. My work focuses on the Pleckstrin ...


Transcriptional Co-Repressor Response Of Arabidopsis Thaliana To Different Abiotic Stress, Bhuwan Guragain Dec 2013

Transcriptional Co-Repressor Response Of Arabidopsis Thaliana To Different Abiotic Stress, Bhuwan Guragain

University of New Orleans Theses and Dissertations

Plants adapt to the complex environmental challenges by regulating their gene expression. Analyses of plant genomes have identified many genes that are either expressed or repressed during environmental stress. However we do not have much information on gene repression. Transcriptional repression in Arabidopsis thaliana is caused by co-repressors that lack the DNA binding domain and are recruited by transcription factors to regulate target gene expression. The Sridhar lab has identified co-repressors SLK1, SLK2, and LUH, which prevent the expression of stress response genes under non-stress conditions. Arabidopsis transgenic lines expressing the GUS under the control of co-repressor’s promoter were ...


The Effects Of Ecological Context And Individual Characteristics On Stereotyped Displays In Male Anolis Carolinensis, Catherine Policastro Dec 2013

The Effects Of Ecological Context And Individual Characteristics On Stereotyped Displays In Male Anolis Carolinensis, Catherine Policastro

University of New Orleans Theses and Dissertations

Displays are ubiquitous throughout the animal kingdom. While many have been thoroughly documented, the factors affecting the expression of such displays are still not fully understood. We tested the hypotheses that display production would be affected by ecological context (i.e. the identity of the receiver) and intrinsic qualities of the signaler (i.e. heavyweight and lightweight size class) in the green anole lizard, Anolis carolinensis. Our results supported these predictions and show that a) ecological context, specifically displaying to conspecifics, has the greatest impact on display production; b) size class influenced display rate with heavyweight males displaying more to ...


Effects Of Prescribed Burns On Grassland Breeding Birds At Mississippi Sandhill Crane National Wildlife Refuge, Elizabeth A. Ruffman Dec 2013

Effects Of Prescribed Burns On Grassland Breeding Birds At Mississippi Sandhill Crane National Wildlife Refuge, Elizabeth A. Ruffman

University of New Orleans Theses and Dissertations

There has been a critical decline in grassland bird populations due to habitat fragmentation and deterioration, and suppression of natural fires. Alteration of the disturbance cycle may lead to changes in vegetation structure and thus habitat suitability for breeding grassland birds. Management practices at the Mississippi Sandhill Crane National Wildlife Refuge, including the use of prescribed fire, are in need of evaluation. My study asked what frequency of prescribed burns is necessary to support breeding grassland birds and whether vegetation structure varies among burn units. In this study, bird abundance and species richness did not differ significantly among burn units ...


Statistical And Comparative Phylogeography Of Mexican Freshwater Taxa In Extreme Aquatic Environments, Lyndon M. Coghill Dec 2013

Statistical And Comparative Phylogeography Of Mexican Freshwater Taxa In Extreme Aquatic Environments, Lyndon M. Coghill

University of New Orleans Theses and Dissertations

Phylogeography aims to understand the processes that underlie the distribution of genetic variation within and among closely related species. Although the means by which this goal might be achieved differ considerably from those that spawned the field some thirty years ago, the foundation and conceptual breakthroughs made by Avise are nonetheless the same and are as relevant today as they were two decades ago. Namely, patterns of neutral genetic variation among individuals carry the signature of a species’ demographic past, and the spatial and temporal environmental heterogeneity across a species’ geographic range can influence patterns of evolutionary change. Aquatic systems ...


Assessing The Responses Of Adult, Juvenile, And Larval Fish Assemblages To The Closure Of The Mississippi River Gulf Outlet, An Artificial Tidal Pass In Southeastern Louisiana, Rebecca Weatherall Cope Dec 2013

Assessing The Responses Of Adult, Juvenile, And Larval Fish Assemblages To The Closure Of The Mississippi River Gulf Outlet, An Artificial Tidal Pass In Southeastern Louisiana, Rebecca Weatherall Cope

University of New Orleans Theses and Dissertations

The creation of the Mississippi River Gulf Outlet (MRGO) in 1963 by the U.S. Army Corps of Engineers (USACE) represents one of the most catastrophic, anthropogenic stressors ever to impact the Lake Pontchartrain estuary in southeastern Louisiana, USA. The artificial tidal pass provided a direct route from the Gulf of Mexico to New Orleans. It allowed for high saline waters to enter Lake Pontchartrain, resulting in detrimental changes to the biotic community of the Lake and surrounding wetland areas. In July 2009, the USACE closed the MRGO in hopes of restoring natural ecosystems. This study assesses changes in the ...


Molecular And Genetic Analysis Of A Conserved Transcription Factor With A Role In Promoting The Completion Of Cytokinesis In Schizosaccharomyces Pombe, Bidhan Chakraborty Dec 2013

Molecular And Genetic Analysis Of A Conserved Transcription Factor With A Role In Promoting The Completion Of Cytokinesis In Schizosaccharomyces Pombe, Bidhan Chakraborty

Electronic Thesis and Dissertation Repository

The fission yeast Schizosaccharomyces pombe activates regulatory networks that promote the faithful execution of cytokinesis in response to drugs that perturb the cytokinesis machinery. In order to identify novel components of these networks, a screen for mutants hyper-sensitive to the actin depolymerizing drug LatrunculinA (LatA) was previously performed. This screen identified a transcription factor, Pap1p, which is orthologous to the mammalian stress activated transcription factor, AP-1. Through molecular and genetic analysis, I showed that the deletion mutant of pap1 is sensitive to LatA and that it cannot maintain the integrity of the actomyosin ring upon LatA treatment leading to cytokinesis ...


Characterization Of The Alternative Oxidase From The Psychrophilic Green Alga Chlamydomonas Sp. Uwo241, Michael Sj Inman Dec 2013

Characterization Of The Alternative Oxidase From The Psychrophilic Green Alga Chlamydomonas Sp. Uwo241, Michael Sj Inman

Electronic Thesis and Dissertation Repository

The alternative oxidase (AOX) was studied in the psychrophilic green alga Chlamydomonas sp. UWO241. AOX is the sole component of the alternative pathway of mitochondrial electron transport and is present in all plant and algal species. In silico analysis of the deduced protein sequence of the cloned AOX cDNA showed that the UWO241 protein has lower amounts of proline and higher amounts of lysine and tryptophan compared to the AOX sequence of the mesophilic alga C. reinhardtii. These changes have been seen in other studies of cold-adapted enzymes. Interestingly, unlike C. reinhardtii, AOX transcript abundance in UWO241 did not increase ...


Phospholipase D- And Phosphatidic Acid-Mediated Signaling In Plant Response To Abscisic Acid And Reactive Oxygen Species, Liang Guo Dec 2013

Phospholipase D- And Phosphatidic Acid-Mediated Signaling In Plant Response To Abscisic Acid And Reactive Oxygen Species, Liang Guo

Dissertations

The Arabidopsis genome has 12 phospholipase D (PLD) genes that are classified into six types, PLDα, PLDβ, PLDγ, PLDδ, PLDε, and PLDζ, based on sequence similarities, domain structures, and biochemical properties. Phosphatidic acids (PA) produced by PLDs have been identified as important lipid signaling molecules in cell growth, development, and stress responses. This study was undertaken to determine the role of PLD and PA in plant response to abscisic acid (ABA) and reactive oxygen species (ROS). The lipid mediator PA was found to interact with sphingosine kinases (SPHKs) in Arabidopsis. Two unique SPHK cDNAs were cloned and expressed. Both SPHKs ...


Characteristics And Effects Of Muscular Dystrophy In Broiler Chickens, Kayla N. Smith Dec 2013

Characteristics And Effects Of Muscular Dystrophy In Broiler Chickens, Kayla N. Smith

Honors Theses

Muscular dystrophy is very common among broilers, which are chickens raised specifically for meat production. When dystrophic chickens are mated, or when normal and dystrophic chickens are mated, it is very likely that at least fifty percent of the clutch will end up with muscular dystrophy since it is inherited as a co-dominant disorder (Fujiwara et al., 2009). For my study, eggs were obtained from a local farm, and allowed to hatch. Among these hatchings were chicks who exhibited some traits of muscular dystrophy. It is possible that the parents of the seemingly dystrophic chicks may carry the mutated WWP1 ...


Prey Capture In Response To Removing Cilia From Venus Flytraps, Emily Marchini Dec 2013

Prey Capture In Response To Removing Cilia From Venus Flytraps, Emily Marchini

Honors Theses

Carnivorous plants are able to attract, trap, and digest insects. Different types of traps target different prey. There are sticky traps, pitfall traps, and active steel traps (Gibson, 1991). Although this may seem to be a rare modification it appears that there are six origins of carnivorous plants in different groups of angiosperms (Albert et al., 1992). These results indicate that not only were there multiple origins of carnivorous plants, there were also multiple origins of the same trapping mechanism; for example, the flypaper trap has five origins and the pitcher trap has three (Albert et al., 1992). Multiple origins ...


Alzheimer's Disease And Circadian Rhythms In A Drosophila Model, Lauren Alejo Dec 2013

Alzheimer's Disease And Circadian Rhythms In A Drosophila Model, Lauren Alejo

Honors Theses

Sleep disorders are commonly reported in Alzheimer's disease, a debilitating, age-related neurodegenerative disorder that affects neurons in the brain. Recently, several studies have suggested a role for sleep abnormalities and the internal "body clock" known as the circadian system, in the disease onset and progression. Since most of the data has been collected from mammals with complex neural circuitry, the molecular and cellular mechanisms that integrate the two neural networks are still limited. This study examined the relationship between circadian rhythmicity and Alzheimer's Disease presentation using a Drosophila model. Flies were crossed using the GAL4-UAS system to display ...


Effectiveness Of Gaming Systems On Balance In Older Individuals, Mary E. Anderson Dec 2013

Effectiveness Of Gaming Systems On Balance In Older Individuals, Mary E. Anderson

Honors Theses

Balance training using gaming systems, called exergaming, is a rising trend for reducing fall risk in older individuals. Previous studies have conducted research pertaining to gaming systems and traditional balance training, however there is a lack of comparison between gaming systems. This study was performed to determine the effectiveness of two gaming systems, the Wii Fit and Xbox Kinect, as compared to traditional balance training. This study was performed with subjects (N=5) over the age of 65, in good health, randomly placed in one of the three balance training groups: Wii Fit (n=2), Xbox Kinect (n=2), and ...


Cleft Lip And Palate Repair: Bridging The Gap Between Genetics And Oral Health: An Individual Case Study, Duncan Perry Dec 2013

Cleft Lip And Palate Repair: Bridging The Gap Between Genetics And Oral Health: An Individual Case Study, Duncan Perry

Honors Theses

Cleft Lip and Palate Syndrome (CL/P) is a condition that occurs in vitro, during fetal development. Because the majority of CL/P cases occur in underdeveloped nations, the families of CL/P patients are typically characterized by impoverished homes. These are the very people who cannot afford to pay for folic acid supplements, or reconstructive and reparative surgeries. Many families struggle under the extreme pressure of raising a child with CL/P. In these nations, the child often cannot receive the proper care they need, and usually are the product of unsuccessful surgeries. The stress of a CL/P ...


Seed Bank Study Of The Effect Of Land Use On Vegetation Diversity In Carolina Bays, Maeve Snyder Dec 2013

Seed Bank Study Of The Effect Of Land Use On Vegetation Diversity In Carolina Bays, Maeve Snyder

Honors Theses

The intent of my honors thesis is to conduct a comparative analysis of seed banks between Carolina bays of high and low human impact. My study will use Geographic Information Systems (GIS) to characterize types of land use within Carolina bays and in 250-m buffer zones. Representative bays from high-impact and low-impact classifications will be chosen as study sites, and seed bank samples will be collected and germinated. While it is beyond the scope of my study to identify a mechanism by which it occurs, I will investigate the presence of a relationship between land use and bay vegetation biodiversity.


Prevention Of Increasing Burnout Levels Among Physicians Of Different Specialties And Doctoral Degrees, Taylor Lewis Dec 2013

Prevention Of Increasing Burnout Levels Among Physicians Of Different Specialties And Doctoral Degrees, Taylor Lewis

Honors Theses

Burnout rates are steadily increasing among physicians all over the world (Sime, Quick, Saleh, & Martin, 2007). Burnout is defined as high levels of emotional exhaustion (EE), high levels of depersonalization (DP), and low levels of personal achievement (PA) (Ionita, Copotocan, & Copotoiu, S., 2011). These burnout rates are directly correlated with many factors, such as high levels of emotional exhaustion and stress (McManus, Winder, & Gordon, 2002), little experience in the medical field (Keswani, Taft, Coté, & Keefer, 2011), and long work hours which leads to sleep deprivation and fatigue (Jackson, 1999). These moderate to high levels of burnout are detrimental to the ...


Functional Response Of Venus Flytraps (Dionaea Muscipula), Amber D. Martin Dec 2013

Functional Response Of Venus Flytraps (Dionaea Muscipula), Amber D. Martin

Honors Theses

The feeding response of Venus flytraps (Dionaea muscipula) is widely studied, and many studies have considered the selectivity of prey capture in D. muscipula (Lichtner and Williams, 1977; Hutchens and Luken, 2009). The consumption rate of an organism relative to the abundance or availability of its food is known as that organism's functional response (Jeschke et al., 2004). In this study, a functional response curve will be constructed for D. muscipula based on its response to increasing availability of small prey in the laboratory. Knowledge of functional responses is important for various fields of biology including population biology, evolutionary ...


The Genetic Architecture And Evolution Of Brain Cortical Folding In A Pedigreed Primate Population, Elizabeth Grace Atkinson Dec 2013

The Genetic Architecture And Evolution Of Brain Cortical Folding In A Pedigreed Primate Population, Elizabeth Grace Atkinson

Arts & Sciences Electronic Theses and Dissertations

Elevated neurological faculty, related to the dramatic increase in brain volume, is a hallmark of the primates. Cognitive capacity, the processing power and speed of the brain, is directly related to the number of neurons in the cerebral cortex and the connectivity network underlying information processing in the brain. Increased cortical folding (gyrification) allows for more neurons to be contained within the volume of the braincase and the arrangement of folds and ridges across the cerebral cortex is an indication of the underlying neural network connecting regions. The goal of this dissertation is to develop a better understanding of the ...


A Novel Rcc1-Like Protein Is A Crucial Regulator Of The Intraerythrocytic Cycle Of The Human Malaria Parasite, Plasmodium Falciparum., Marcus Davon Skaflen Dec 2013

A Novel Rcc1-Like Protein Is A Crucial Regulator Of The Intraerythrocytic Cycle Of The Human Malaria Parasite, Plasmodium Falciparum., Marcus Davon Skaflen

Masters Theses

Malaria is a deadly infection caused by a single celled protozoan of the Plasmodium genus. Plasmodium spp. are transmitted to humans by mosquitoes, and initially invade the liver, but the disease is caused by the blood stage of the infection. Approximately 500 million cases of malaria are documented annually and over 1 million of those result in death. Plasmodium falciparum is the most lethal of five species known to infect humans. To further compound this problem, drug-resistant parasite strains have been documented for every currently available antimalarial drug, making the need to identify new drug targets more urgent than ever ...


Effects Of Excitation Pressure On Variegation And Global Gene Expression In Arabidopsis Thaliana, Rainer Bode Dec 2013

Effects Of Excitation Pressure On Variegation And Global Gene Expression In Arabidopsis Thaliana, Rainer Bode

Electronic Thesis and Dissertation Repository

I assessed the effects of photosystem II excitation pressure on chloroplast biogenesis and leaf sectoring in the Arabidopsis thaliana variegated mutants im, spotty, var1, var2, chs5 and atd2. The plants were grown under varying degrees of excitation pressure induced by growth at increasing irradiance at different temperatures and the extent of variegation was quantified throughout the plant’s development. I found that the degree of variegation was positively correlated with excitation pressure, regardless of whether high light or low temperature was used to induce increased excitation pressure in all the mutants tested. This was irrespective of whether the mutation causing ...


Mitochondrial Dysfunction And Reactive Oxygen Species In Neurodegeneration, Leah Farina Dec 2013

Mitochondrial Dysfunction And Reactive Oxygen Species In Neurodegeneration, Leah Farina

Honors Theses

Neurodegeneration leads to the loss of normal anatomy and physiology of the neuronal system in a human body. The hallmark of most neurodegenerative diseases is abnormal folding and accumulation of proteins within neuronal cell bodies that is not present in healthy people. These changes in normal protein metabolism often lead to neuronal cell death and failure of the affected regions of the central nervous system. Abnormal protein accumulation may be attributed to dysfunctional mitochondria and damage by reactive oxygen species. In addition to causing altered protein accumulation, dysfunctional mitochondria and an excess of reactive oxygen species also lead to errors ...


Fumarate Inhibits Tnf-Α Release, Megan Kelly Dec 2013

Fumarate Inhibits Tnf-Α Release, Megan Kelly

Seton Hall University Dissertations and Theses (ETDs)

Vertebrates utilize two forms of immunity to combat pathogens. Innate immunity is considered the first line of defense that utilizes immediate action and three barriers. Innate immunity responses typically occur within minutes of pathogen exposure resulting in cellular receptor activation and acute pro-inflammatory cytokine release. Inflammatory macrophages engage bacterial endotoxins, including Gram-negative lipopolysaccharide (LPS) and Gram-positive lipoteichoic acid (LTA). And respond by releasing tumor necrosis factor-α (TNF-α). While beneficial for neutralizing acute pathogen exposure, prolonged TNF-α release results in chronic inflammation and tissue damage. In the present study, we examined two methylated derivatives of a citric acid cycle intermediate, fumarate ...


Foraging Ecology, Sexual Selection, And Divergence In Sunfish (Lepomis Spp.), Scott F. Colborne Dec 2013

Foraging Ecology, Sexual Selection, And Divergence In Sunfish (Lepomis Spp.), Scott F. Colborne

Electronic Thesis and Dissertation Repository

The origins of novel traits and their contribution to biodiversity have long been of interest to biologists. My research focused on the links between foraging ecology and both natural and sexual selection, and how these mechanisms interact to shape the phenotypic diversification of natural populations. Using bluegill (Lepomis macrochirus) and pumpkinseed sunfish (Lepomis gibbosus), I examined three major questions: 1) how are diet and morphological variation related to alternative reproductive tactics in bluegill; 2) are sexual selection and disruptive selection driving divergence between foraging ecomorphs in pumpkinseed; and 3) how are human-induced changes to prey communities affecting foraging ecology of ...


Evolution And Functional Morphology Of The Cephalic Lobes In Batoids, Samantha Lynn Mulvany Dec 2013

Evolution And Functional Morphology Of The Cephalic Lobes In Batoids, Samantha Lynn Mulvany

Graduate Theses and Dissertations

Cephalic lobes are unique structures derived from the anterior pectoral fins, found in select myliobatid stingrays. Many benthic batoids utilize undulatory locomotion and use their pectoral fins for both locomotion and prey capture. Pelagic myliobatids that possess cephalic lobes utilize oscillatory locomotion, using their pectoral fins to locomote and their cephalic lobes for prey capture. Despite differences in habitat usage and locomotor modes, these batoids feed on very similar benthic organisms. The purpose of this study was to 1.) compare the morphology of the cephalic lobes and anterior pectoral fins in lobed and lobeless species, looking at skeletal elements, musculature ...


The Evolution Of Floral Symmetry Across The Plant Order Lamiales, Jinshun Zhong Dec 2013

The Evolution Of Floral Symmetry Across The Plant Order Lamiales, Jinshun Zhong

Dissertations

Bilaterally symmetrical corollas have evolved independently numerous times from radially symmetrical ancestors and are thought to represent adaptation to specific pollinators. However, evolutionary losses of bilateral symmetry have occurred sporadically in different lineages. CYC2-like and RAD-like are genes needed for the normal development of bilateral symmetry in snapdragon corollas. However, exactly how changes in the floral symmetry patterning genes correlate with the origin and loss of floral bilateral remains poorly known. To address this question, a densely sampled phylogeny of CYC2-like genes across the order Lamiales was inferred and calibrated. The expression patterns of these genes in early diverging and ...


The Effect Of Testosterone On The Spring Migratory Phenotype Of A North American Songbird (Zonotrichia Albicollis), Caitlin L. Vandermeer Dec 2013

The Effect Of Testosterone On The Spring Migratory Phenotype Of A North American Songbird (Zonotrichia Albicollis), Caitlin L. Vandermeer

Electronic Thesis and Dissertation Repository

In passerines, the endocrine modulators responsible for seasonal changes in migratory behaviour and physiology are unclear. Spring photoperiods alter androgen levels, influencing muscle mass and fat deposition rates to power migration, as well as enhance nocturnal migratory restlessness activity (Zugunruhe). My study compared physiological indicators and migratory restlessness in castrated and intact white-throated sparrows (Zonotrichia albicollis) following photoperiod and hormone manipulation. Nocturnal restlessness activity was highest in migratory intact males or migratory castrated males that received testosterone replacement. Liver mass decreased in all photostimulated migratory groups regardless of testosterone treatment. Likewise, oxidative enzyme activity was unrelated to testosterone, but was ...


Elucidating The Factors That Modulate The Distribution Of Avian Haemosporida Parasites Across A Community Of Hosts, Matthew Christopher Ikaika Medeiros Dec 2013

Elucidating The Factors That Modulate The Distribution Of Avian Haemosporida Parasites Across A Community Of Hosts, Matthew Christopher Ikaika Medeiros

Dissertations

Parasites are heterogeneously distributed across host species, host populations, and host individuals within populations. A primary aim of infectious disease ecology seeks to uncover the factors that drive this heterogeneity. At a fundamental level, host infection is determined by exposure and susceptibility to a pathogen. My dissertation explores how evolutionary and ecological forces associated with these fundamental determinates of infection shape variation in parasite host breadth and host infection status. Here, I focus on a community of vector-borne avian Haemosporida parasites among suburban birds of Chicago, IL. These parasites exhibit strong variation in their distribution among available hosts, and provide ...


Exploring The Local And Regional Effects Of Plant Diversity On Plant Herbivore Interactions, Diego Salazar Dec 2013

Exploring The Local And Regional Effects Of Plant Diversity On Plant Herbivore Interactions, Diego Salazar

Dissertations

The study of biological diversity and its effects on ecosystem functioning and species interactions has always been a fundamental part of biology. The accelerating loss of species in conjunction with an increasing change in the natural environment has underlined the importance of the role that biodiversity has on the evolutionary and ecological dynamics of natural systems. In this dissertation I explore the effect of local and regional patterns of plant diversity in plant-herbivore interactions. Furthermore, this work goes beyond the classical concepts of taxonomical diversity and investigates the role of phylogenetic and chemical diversity on plant-herbivore interactions. In chapter one ...


The Response Of Naive Channel Catfish To Chemical Cues Associated With Predation, Marinda Coulter Dec 2013

The Response Of Naive Channel Catfish To Chemical Cues Associated With Predation, Marinda Coulter

Theses and Dissertations

The chemosensory abilities of fishes, are important in order to understand how prey can perceive and avoid predators. Predator-naïve Channel catfish were exposed to four extracts over eight sessions (naïve bass water (NBW), bass that were fed catfish water (BFCW), catfish skin extract (CS), and naïve bass water paired with catfish skin extract (CO)) to determine whether they have an innate predator response to potential chemical cues indicating possible predation risk. Movement was quantified as grid squares crossed, directional changes, and tail beats. Response increased during the first minute following stimulus injection and decreased during the second minute. Channel catfish ...


Who's Your Daddy? A Study Of Extra-Pair Copulation And Mating Behaviors Of Protonotaria Citrea, Morton Catherine Heidrich Dec 2013

Who's Your Daddy? A Study Of Extra-Pair Copulation And Mating Behaviors Of Protonotaria Citrea, Morton Catherine Heidrich

Theses and Dissertations

Mating behavior has a profound impact on reproductive success and the resulting genetic structure of offspring. Extra-pair copulation is a widely observed behavior within avian species. This study explored the genetic effects of mating behaviors of Prothonotary warblers, Protonotaria citrea (Parulidae), using co-dominant microsatellite markers. Prothonotary warblers are migratory songbirds that build nests in cavities, commonly found in wetland habitats. A set of artificial nest boxes were initiated by Dr Robert Reilly in 2002 in Dutch Gap, Chesterfield County Virginia, USA, a tidal tributary off the James River. From this population, 28 nest boxes were surveyed yielding 47 adults and ...