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Theses/Dissertations

1999

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Full-Text Articles in Life Sciences

The Effect Of Shelterwood-Cut Oak Forestry Practices On Breeding Bird Success, Patrick Lee Collins Dec 1999

The Effect Of Shelterwood-Cut Oak Forestry Practices On Breeding Bird Success, Patrick Lee Collins

Theses, Dissertations & Honors Papers

Populations of breeding birds were studied on two shelterwood-cut oak stands in Buckingham County, Virginia. These two tracts, Harris East and Harris West, were cut in 1996 and a Breeding Bird Census has been conducted on these tracts since 1997. This study is part of an ongoing effort to collect pre-bum data, and document the diversity of avian species utilizing this habitat. The changes in avian species present on the Harris East and Harris West plots is important to know so that the effects of shelterwood-cut forestry practices can be understood better. The data collected showed that, by changing the ...


Analysis Of Cell Polarity Signaling In C. Elegans: A Dissertation, Christian Ernest Rocheleau Dec 1999

Analysis Of Cell Polarity Signaling In C. Elegans: A Dissertation, Christian Ernest Rocheleau

GSBS Dissertations and Theses

During embryonic development of the nematode Caenorhabditis elegans, cell fates are specified by asymmetric segregation of cell fate determinants and via cell-cell signaling events. Specification of the eight-cell stage blastomere E, the endoderm progenitor cell, requires both cell signaling and asymmetric cell division. At the four-cell stage, a polarity-inducing signal from the P2 cell is required for the EMS cell to divide asymmetrically to produce an anterior daughter MS, and posterior daughter E. In the absence of signal, the EMS cell divides symmetrically to produce two daughters that adopt the MS fate. This thesis describes the identification and analyses ...


Dna Fingerprinting: Identification Of Organisms Using The Polymerase Chain Reaction And Various Primers, Vera Santoleri Dec 1999

Dna Fingerprinting: Identification Of Organisms Using The Polymerase Chain Reaction And Various Primers, Vera Santoleri

Biology Master’s Theses

The study undertaken involved small scale DNA isolation from eight different fruits using a modified technique written for leaf material. Genetic analysis of this extracted DNA was performed by PCR. Four primers known to target specific DNA sequences were utilized: Analu, Bactoribo, HHFl, and Mitocox. PCR with the Analu, HHFl, and Mitocox primers resulted in a unique pattern of bands that enabled each fruit to be differentiated. Since one major band was observed with the Bactoribo primers and the size of that amplified DNA fragment was either the same or very similar for each fruit, they could not be distinguished ...


Survey Of Amphibians And Reptiles In Powhatan County, Virginia With Selected Natural History Notes, Jason Daniel Gibson Dec 1999

Survey Of Amphibians And Reptiles In Powhatan County, Virginia With Selected Natural History Notes, Jason Daniel Gibson

Theses, Dissertations & Honors Papers

This thesis establishes baseline data on the herpetofauna that exist in Powhatan county. No systematic study of the amphibians and reptiles in Powhatan county has ever been conducted. The methods used in this survey include hand capture, egg mass observations, road kill collections, and frog call surveys. Documentation of each species collected included measurements, photographs, range maps, and tape recordings.

This survey produced 8 species of salamanders, 11 anurans, 6 turtles, 4 lizards, and 14 snakes. Based on records in the Atlas of Amphibians and Reptiles of Virginia (Mitchell & Reay, 1999), 11 new county records were discovered. This brings county ...


Effect Of Calendaring On Flexographic Print Ability Of Linerboard, William P. Woods Dec 1999

Effect Of Calendaring On Flexographic Print Ability Of Linerboard, William P. Woods

Master's Theses

Today's corrugated board end user demands a higher quality than ever. Retailing techniques are moving toward so-called ''value-added packaging," which consists of high quality printed packaging that works to sell the product from the shelf. To preserve'its dominance in the corrugated market, the flexographic printing industry must find new ways to cost effectively satisfy the new demands.

One possibility is the use of an additional calendering step to give the linerboard better printability. This study evaluated two different types of calenders: the shoe (extended nip) calender and the hot/soft nip calender (Appendix A). The test material was ...


Reciprocal Transplantation Of The Desert Soil Crust: Can It Be Done?, Christina D. Cole Dec 1999

Reciprocal Transplantation Of The Desert Soil Crust: Can It Be Done?, Christina D. Cole

UNLV Theses, Dissertations, Professional Papers, and Capstones

A 100 meter X 100 meter study site was chosen at White Rock Springs in Red Rock Canyon National Conservation Area, Nevada. To examine Syntrichia caninervis, a desert crustal moss, reciprocal transplantations (20 mm and 40 mm diameter cores) were performed between shaded to shaded, exposed to exposed, and shaded to exposed microsites to determine if it was possible to transplant the study organism with reasonable survivorship. Transplants were inspected following rain events for percent hydration, number of dead stems, and change in percent cover. Data indicated that there was a low mortality rate of S. caninervis stems, and few ...


Fundamental Study Of The Coating And Basesheet Influences On Gloss In Relationship To Coated Inkjet Papers, Raja Ramakrishnan Dec 1999

Fundamental Study Of The Coating And Basesheet Influences On Gloss In Relationship To Coated Inkjet Papers, Raja Ramakrishnan

Master's Theses

The use of fumed metallic oxides for glossy ink jet media offers alternatives to expensive cast coated and extruded film methods. Fumed metallic oxide pigments provide a high internal porosity and packing porosity that enable the rapid diffusion of liquid inks into the coating layer. Their small particle size (0.1-0.3μm), enable the development of gloss. However, calendering is needed to develop gloss values in the range of cast coated and extruded film grades.

Calendering of coated sheet reduces production speed, adds additional handling and rewinding cost (if performed off-line) and reduces the bulk and porosity of the coating ...


Assessing The Potential Of Prtoll As A Bioremedial Agent Using Non-Isotopic, 16s-Rrna Targeted Oligonucleotide Probes, Jason Stricker Oct 1999

Assessing The Potential Of Prtoll As A Bioremedial Agent Using Non-Isotopic, 16s-Rrna Targeted Oligonucleotide Probes, Jason Stricker

Environmental Science and Ecology Theses

This investigation focuses on the bioremediation of toluene in freshwater, anaerobic conditions by the recently isolated bacterial strain PRTOL1, and the development of a rapid and efficient quantitative method using 16S-rRNA targeted, fluorescently labelled oligonucleotide probes to directly detect and monitor this strain in situ. This investigation also focuses on the application of this method to determine the effects of amending the soil with various carbon sources on the population of PRTOL1.


Development Of A Novel Method For Phenol Detection Using High Performance Thin Layer Chromatography, Danette Young Carlton Aug 1999

Development Of A Novel Method For Phenol Detection Using High Performance Thin Layer Chromatography, Danette Young Carlton

Theses, Dissertations & Honors Papers

The purpose of this study was to develop a more efficient method to test for phenol in both wastewater and non-wastewater. Phenol is a caustic and toxic contaminant that may be present in our water supplies. Phenol is used in various resins, pharmaceuticals, plastics and disinfectants. Environmental Protection Agency methods are available to test for phenol using gas chromatography along with mass spectrometry or flame ionization detection. Each of these methods can be a long, involved process consisting of extraction, many sample injections, and interference problems. This method developed an easier and less complicated approach of testing for phenol in ...


Taxa Determination By The Polymerase Chain Reaction: A Survey, Jeffrey S. Kiggins Aug 1999

Taxa Determination By The Polymerase Chain Reaction: A Survey, Jeffrey S. Kiggins

Biology Master’s Theses

The objective of this research was to survey a number of DNA samples from various organisms, using different primers, to determine what differences exist between organisms relative to each primer. This survey would help to contribute information for the following:

1. Educational purposes. A student receiving an unknown DNA should be able to identify the organism to particular taxa.

2. Determine the presence of PCR products from DNA and primers that would not typically be used together. That is, a survey of unconventional combinations.

3. Determine what differences exist between the snails, L. saxatalis and L. compressa using this particular ...


Role Of Mitogen-Activated Kinases In Cd40-Mediated T Cell Activation Of Monocyte/Macrophage And Vascular Smooth Muscle Cell Cytokine/Chemokine Production, Denise M. Milhorn Aug 1999

Role Of Mitogen-Activated Kinases In Cd40-Mediated T Cell Activation Of Monocyte/Macrophage And Vascular Smooth Muscle Cell Cytokine/Chemokine Production, Denise M. Milhorn

Electronic Theses and Dissertations

This dissertation represents efforts to determine the functional consequences acquired by vascular smooth muscle cells (SMC) in response to CD40 ligation by activated CD154+ T cells, and to elucidate components of the signaling pathway(s) activated in response to CD40 signaling in both monocytes and SMC. To study the consequences of CD40 stimulation, primary human monocytes and aortic SMC were treated with plasma membranes purified from CD154 + , CD4+ T cells. The results presented in this dissertation demonstrate that SMC, like monocytes/macrophages, are capable of interacting with T cells in a manner that results in reciprocal activation events. SMC were ...


Maternal Employment And Dietary Quality Of Children Aged 42-60 Months, Lynn A. Samson Aug 1999

Maternal Employment And Dietary Quality Of Children Aged 42-60 Months, Lynn A. Samson

Masters Theses

(From the Summary): Abbreviated Maternal employment is becoming more the usual than an exception. Employment can be beneficial for mothers, providing satisfaction and increasing self-esteem. On the other hand, it can be stressful juggling between the roles of employee and mother. Other family members might help ease some of the burden, but mothers still bear most of the responsibility of child care and household responsibilities. Working mothers have less time to spend on food and nutrition. In most cases, the diets of children whose mothers are employed do not differ from children of mothers who are not employed, but this ...


Capture And On-Site Release Of Nuisance Black Bears And Survival Of Orphaned Black Bears Released In The Great Smoky Mountains, Jay Edwin Clark Aug 1999

Capture And On-Site Release Of Nuisance Black Bears And Survival Of Orphaned Black Bears Released In The Great Smoky Mountains, Jay Edwin Clark

Masters Theses

Since the establishment of Great Smoky Mountains National Park (GSMNP) in 1934, interactions between black bears (Ursus americanus) and visitors have been a regular occurrence. Prior to 1990, capture and relocation was the primary management alternative for nuisance bears in GSMNP. Since 1990, wildlife biologists in GSMNP have used capture and on-site release as an aversive conditioning technique for nuisance black bears. This technique involves capturing and immobilizing bears that frequent developed areas, collecting biological data, and releasing the bears back into the same area. The premise of this technique is to reinforce the natural fear of humans and thereby ...


Signaling Mechanisms For Muscarinic Receptor-Mediated Coronary Vasoconstriction In Isolated Rat Hearts, Yi Zhang Aug 1999

Signaling Mechanisms For Muscarinic Receptor-Mediated Coronary Vasoconstriction In Isolated Rat Hearts, Yi Zhang

Electronic Theses and Dissertations

The signaling mechanisms for muscarinic receptor-mediated vasoconstriction in coronary resistance arteries were studied in KCl-arrested isolated rat hearts perfused at a constant flow rate. The cholinergic agonists acetylcholine and bethanechol were given by bolus injection or constant infusion. The coronary vascular resistance was monitored by measuring the changes in perfusion pressure. The selective muscarinic agonist bethanechol caused a similar vasoconstrictor response as ACh, but with less potency and efficacy. Bolus injection of bethanechol evoked a phasic vasoconstriction in a dose-dependent manner, while infusion of bethanechol evoked a tonic vasoconstriction without producing tachyphylaxis. Coronary vascular responses to bethanechol were further examined ...


Universal Design And Nature Trails: Balancing Accessibility, Site Integrity, And The Recreation Experience, Steve Kirkindall Aug 1999

Universal Design And Nature Trails: Balancing Accessibility, Site Integrity, And The Recreation Experience, Steve Kirkindall

Electronic Theses and Dissertations

This study addressed the application of Americans With Disabilities Act (ADA) guidelines in outdoor recreation settings. The purpose was three-fold: to develop site- and user-friendly design principles for non-urban, accessible nature trails; to design and construct a trail based on those principles; and to evaluate both principles and trail by surveying trail users. The Delphi Method was employed with a national panel to assess current expert opinion regarding accessibility and trails. Fifty-nine principles were developed from combined results of panel deliberations and applied experience gained constructing a prototype trail utilizing the universal design concept. Trail users rated the importance of ...


Effect Of Various Starch Sources On The Silicone Holdout Of Release Paper, Chandrasekaran Duraiswamy Aug 1999

Effect Of Various Starch Sources On The Silicone Holdout Of Release Paper, Chandrasekaran Duraiswamy

Master's Theses

Siliconized papers are widely being used from food and packaging industry to pressure sensitive label industry and their use has increased considerably over the last decade. Surface and structural properties of the sheet influence the silicone hold out and release properties.

Surface sizing of the sheet is done prior to silicone coating. The objective of this research is to find their film forming capabilities, fluid hold out of various starches, and the effect of the various calendering process on the release properties was also studied.

No significant difference between the starch sources were observed, except for the modified com starch ...


Reactive Oxygen Species Generated By Depletion Of Cellular Glutathione Mediate Cadmium Cytotoxicity, Jun Xu Aug 1999

Reactive Oxygen Species Generated By Depletion Of Cellular Glutathione Mediate Cadmium Cytotoxicity, Jun Xu

Master's Theses

Studies were put forth to test whether or not reactive oxygen species, which are influenced by the cellular level of reduced glutathione, mediate the toxicity of cadmium. Cadmium has been a focal point in the health care community in the past few years because it has been shown to induce the development of tumors, interfere with cellular signaling, and cause damage to organs, tissues, and biomolecules. Although various studies and theories have been proposed, little direct evidence, especially from studies utilizing primary cells in culture, has accumulated to show the mechanism by which the cadmium induced toxicity occurs.

Our studies ...


Transcriptional Mapping Within A 1.7 Megabase Region Of Human Chromosome 17q21, Alisa C. Plummer Jul 1999

Transcriptional Mapping Within A 1.7 Megabase Region Of Human Chromosome 17q21, Alisa C. Plummer

ETD Collection for AUC Robert W. Woodruff Library

Exon amplification is a method used to identify regions of DNA that contain transcribed sequences. The large cloning capacity of yeast artificial chromosome (YAC) systems and the inability to isolate large intact DNA from YACs introduce limitations to the exon amplification technique. The feasibility of exon amplification from a mega-YAC clone 2001C6 (CEPH) has been analyzed as a means to isolate transcribed sequences and has been used to produce 10 putative exon sequences from human chromosome 17q21. Six of the sequences showed homology to sequences that were previously published in the GenBANK database. Three of the sequences showed no homology ...


The Effects Of Habitat Fragmentation On Amorpha Canescens, A Prairie Forb, And Its Associated Herbivores, Cassandra Kasun Lewis Jul 1999

The Effects Of Habitat Fragmentation On Amorpha Canescens, A Prairie Forb, And Its Associated Herbivores, Cassandra Kasun Lewis

Theses and Dissertations

No abstract provided.


Standardization Of An Immunoassay For The Detection Of Antibodies To B2 Glycoprotein-I, Eric N. Erickson Jr. Jul 1999

Standardization Of An Immunoassay For The Detection Of Antibodies To B2 Glycoprotein-I, Eric N. Erickson Jr.

Seton Hall University Dissertations and Theses (ETDs)

Antiphospholipid Syndrome (APS) is an evolving autoimmune disease with numerous clinical manifestations. APS occurs in two forms: Primary Antiphospholipid Syndrome (PAPS) and secondarily in association with other autoimmune disorders such as Systemic Lupus Erythematosis (SLE). In the brief period since its discovery as a cofactor for anticardiolipin antibodies, p1-glycoprotein I (�GPI; apolipoprotein H) has been recognized as the autoantigen in the absence of anionic phospholipid when appropriately presented to human autoantibodies. Recent studies suggest that this presentation requires the surface-dependent unmasking of a cryptic epitope. Immunoassays of autoantibodies directed against p1GPI, developed through the application of suitable polymeric matrices, may ...


Nitrate Reductase Activity, Nitrate Uptake And Iodine Speciation In The Marine Environment, Chin-Chang Hung Jul 1999

Nitrate Reductase Activity, Nitrate Uptake And Iodine Speciation In The Marine Environment, Chin-Chang Hung

OEAS Theses and Dissertations

The present method for the determination of new production (NP) by measuring the uptake of added 15NO3 suffers from a number of limitations. In an attempt to improve this situation, this research examined the possibility of estimating 15NO3– uptake by measuring the activity of nitrate reductase (NRA). In addition, because it has long been suspected that the biological reduction of IO3 to I may be mediated by nitrate reductase (NR), this research investigated the ability of NR to catalyze the reduction of iodate to iodide.

An improved method for the determination of NRA was developed ...


Tissue-Specific Expression And Steroid Hormone Regulation Of Human Gonadotropin -Releasing Hormone (Hgnrh) Gene In Placental Cells (Jeg -3 Cells), Zhengguang Chen Jul 1999

Tissue-Specific Expression And Steroid Hormone Regulation Of Human Gonadotropin -Releasing Hormone (Hgnrh) Gene In Placental Cells (Jeg -3 Cells), Zhengguang Chen

Theses and Dissertations in Biomedical Sciences

Using JEG-3 cells as an in vitro model, I investigated the mechanisms behind the tissue-specific expression and steroid hormone regulation of the hGnRH gene in the human placenta. The hGnRH upstream promoter was found to be functionally active in JEG-3 cells. The DNA sequence responsible for functioning of the upstream promoter in JEG-3 cells is narrowed to a region between –1048 bp and –730 bp. This DNA fragment contains four elements, which can bind with nuclear extract from JEG-3 cells (but not from GT1-7 cells).

Estradiol (E2) represses the hGnRH upstream promoter activity in JEG-3 cells. This inhibition is receptor-mediated ...


Exploring Participation Processes For Technology Development: Case Studies Of Biotechnology Research And Development Projects In Thailand, Omjai Yuktavetya Jul 1999

Exploring Participation Processes For Technology Development: Case Studies Of Biotechnology Research And Development Projects In Thailand, Omjai Yuktavetya

Engineering Management & Systems Engineering Theses & Dissertations

There is increased awareness in the organizational and management literature that participation can make contributions in technology development. One of the problems in developing biotechnology in Thailand is that only a small portion of publicly funded research and development projects directly result in commercial success. Among the reasons cited (TDRI 1992a) is a lack of collaboration between various stakeholders; therefore, an effective technology policy to support their participation is needed. This research explores current participation processes in biotechnology research and development projects in Thailand in which there were different perspectives among various stakeholders. The quantitative and qualitative methodologies developed here ...


Identification And Characterization Of Determinants Of Head And Neck Tumor Cell Invasion, Yangguan Wu Jul 1999

Identification And Characterization Of Determinants Of Head And Neck Tumor Cell Invasion, Yangguan Wu

Theses and Dissertations in Biomedical Sciences

Head and neck squamous cell carcinoma (HNSCC) is a common malignant disease with poor prognosis. The majority of patients die from local invasion or lymphatic metastasis. The mechanism(s) underlining the invasiveness of HNSCC are poorly understood. Utilizing a panel of HNSCC cell lines previously established in our laboratory, we tested the application and relevance of the three-step hypothesis of tumor invasion to HNSCC and investigated the mechanism(s) pertaining to the regulation of each step in the invasive process. Data presented in this thesis demonstrated that tumor cell invasion in HNSCC is a complex process involving three repeated sequential ...


Use Of Boron Compounds To Reduce Phototyellowing Of Mechanical Pulps, Qilin Zhou Jun 1999

Use Of Boron Compounds To Reduce Phototyellowing Of Mechanical Pulps, Qilin Zhou

Master's Theses

Bleached chemithermomechanical pulps (BCTMP) are replacing chemical pulps such as bleached kraft pulp (BKP) in many paper grades. However, the loss of brightness that arises from exposure to light remains a significant impediment to the wider use of these pulps. Efforts have been made in reducing photoyellowing, but so far no universal, economical method has been found which converts high-yield pulp into a product with brightness stability comparable to that of a bleached chemical pulp.

This study evaluates the ability of sodium borate and/or boric acid to inhibit the yellowing process of paper that was made from BCTMP, with ...


The Influence Of Fumed Metallic Oxides On Coating Structure And Their Relationships To Print Quality, Hyun-Kook Lee Jun 1999

The Influence Of Fumed Metallic Oxides On Coating Structure And Their Relationships To Print Quality, Hyun-Kook Lee

Master's Theses

Aqueous-based coating for paper or paperboard applications use traditional coating pigments such as clay, calcium carbonate, and titanium dioxide. But, for matte coated ink jet papers, amorphous and gelled silicas are used due to their unique morphological properties, which are sponge-like in nature. The unique structure of these pigments provides an internal porosity and packing porosity which enables the rapid diffusion of liquid inks into the coating layer. The rapid uptake of the ink immobilizes the anionic dyes at the surface of the coating, allowing high optical print densities to be achieved.

The particle size of precipitated and gelled silicas ...


Borax As A Strength Additive In Recycling, Siva Krishnagopal Devisetti Jun 1999

Borax As A Strength Additive In Recycling, Siva Krishnagopal Devisetti

Master's Theses

The effect of multiple recycling on strength properties and fiber properties like strength of fiber, weighted average fiber length, fines percentage and water retention values were investigated in this study. Borax was used as a strength aid and swelling agent to improve the flexibility of fiber and strength of recycled paper. Bleached softwood kraft pulp was used to make the hand sheets and recycled repeatedly for up to four cycles. The main objectives were to evaluate the chemically treated pulp and untreated pulp at each stage of recycling, to study the effect of multiple recycling on strength properties of paper ...


Characterization Of Nih-3t3 Mouse Fibroblast Cells Transfected With The Somatostatin Receptor Subtype 2 And The Protein Tyrosine Phosphatase Shp-1, Scott Bradford Cross Jun 1999

Characterization Of Nih-3t3 Mouse Fibroblast Cells Transfected With The Somatostatin Receptor Subtype 2 And The Protein Tyrosine Phosphatase Shp-1, Scott Bradford Cross

Master's Theses

The hormone Somatostatin (SMS) and its analogs have been shown to mediate a variety of cellular actions including inhibition of cellular growth and differentiation of endocrine tumors such as insulinomas and glucagonomas. The mechanisms by which SMS exerts its antitumorigenic effects are poorly understood but are thought to include one of the five Somatostatin receptors (SSTR1-5), and the protein tyrosine phosphatase SHP-1. To determine if the antiproliferative effects of SMS were due to the presence of the SSTR2 receptor or the stimulation of SHP-1, we introduced cDNA's of the SMS receptor SSTR2, SHP-1, and a dominant negative mutant of ...


Transplanting Broodstock Oysters, Crassostrea Virginica, Onto Reconstructed Oyster Reefs To Increase Spat Recruitment In The Piankatank River, Dawn Chentil Sherwood May 1999

Transplanting Broodstock Oysters, Crassostrea Virginica, Onto Reconstructed Oyster Reefs To Increase Spat Recruitment In The Piankatank River, Dawn Chentil Sherwood

Master's Theses

Four oyster reefs were recreated from oyster shells to historical proportions in the Piankatank River from 1993-1995. In December 1997, two of the reconstructed reefs were supplemented with large broodstock oysters from Tangier and Pocomoke Sounds. Since total quantity as well as density ofbroodstock were believed to be limiting factors for recruitment in this river, adding stock was expected to raise spat recruitment. Methods included dive surveys on the reefs, and dredge and patent tong surveys on the natural oyster bars. The recruitment of spat to both reefs and bars was significantly higher in 1998 (p<0.001) than in the previous four years and a positive interaction (p<0.005) was seen between the reefs and the year 1998. Based on these dat~ stock enhancement in the Piankatank River successfully improved recruitment and suggest oyster restoration may be facilitated in other areas of the Chesapeake Bay by strategic enhancement of spawning stocks.


Effects Of Relocating Wild Northern Bobwhites Into Managed Quail Habitat In Middle Tennessee, Jeffrey G. Jones May 1999

Effects Of Relocating Wild Northern Bobwhites Into Managed Quail Habitat In Middle Tennessee, Jeffrey G. Jones

Masters Theses

The effects and feasibility of relocating wild northern bobwhite (Colinus virginianus) quail into managed quail habitat in middle Tennessee were studied on the Maddox farm located in the southeastern portion of Houston County, Tennessee. Data were collected during portions of 2 years beginning in January 1994, and ending in March 1996. The major objectives of the study were to evaluate the effects relocated wild bobwhites might have on an existing resident quail population, and to determine the feasibility of relocating quail wild quail as a potential management tool. Study objectives were accomplished by obtaining data necessary to compare resident and ...