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Theses/Dissertations

Doctoral Dissertations

2017

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Full-Text Articles in Life Sciences

Systematics And Biogeography Of The Cortinarius Violaceus Group And Sequestrate Evolution In Cortinarius (Agaricales), Emma Harrower Dec 2017

Systematics And Biogeography Of The Cortinarius Violaceus Group And Sequestrate Evolution In Cortinarius (Agaricales), Emma Harrower

Doctoral Dissertations

Phylogenetics is a powerful tool used for illuminating the diversity of life on Earth, their evolution and their ecology. I created a multi-gene phylogenetic tree of Cortinarius section Cortinarius and uncovered five previously overlooked species, increasing the number of species in the section from seven to twelve. All members of the clade possess both cheilocystidia and pleurocystidia and possess a pigment known as (R)-39,49-dihydroxybphenylalanine. Ancestral state reconstruction estimated that the ancestral host was most likely an angiosperm, switching hosts when encountering novel host species in new lands, and only C. violaceus associating with the Pinaceae in North America ...


Design And Synthesis Of Analogs Of Myo-Inositol, Serine, And Cysteine To Enable Chemical Biology Studies, Tanei J. Ricks Dec 2017

Design And Synthesis Of Analogs Of Myo-Inositol, Serine, And Cysteine To Enable Chemical Biology Studies, Tanei J. Ricks

Doctoral Dissertations

Phosphorylated myo-inositol compounds including inositol phosphates (InsPs) as well as the phosphatidylinositol polyphosphate lipids (PIPns) are critical biomolecules that regulate many of the most important biological processes and pathways. They are aberrant in many disease states due to their regulatory function. The same is true of the phospholipid phosphatidylserine (PS) which can serve as a marker to begin apoptosis. However, the full scope of activities of these structures is not clear, particularly since techniques that enable global detection and analysis of the production of these compounds spatially and temporally are lacking. With all of these obstacles in ...


Forest Resources For Bioenergy, Gerry Solano Avila Dec 2017

Forest Resources For Bioenergy, Gerry Solano Avila

Doctoral Dissertations

The overall objective of this dissertation is to evaluate forest resources biomass availability for the production of bioenergy. Chapter II provides measures of the impact that the road sustainability criteria have on the supply of feedstock for forest products and bioenergy. A linear cost minimization programming is used in estimating forest biomass supply curves. Chapter III provides estimates on the changes in US timberland acreages overtime and the ability of timberland to meet conventional timber products and woody biomass demand within the conterminous United States. Chapter IV utilizes the Biofuels Facility Location Analysis Modeling Endeavor (BioFLAME), a Geographic Information System ...


Mediation And Moderation Of Intergenerational Epigenetic Effects Of Trauma, Stefanie Renee Pilkay Dec 2017

Mediation And Moderation Of Intergenerational Epigenetic Effects Of Trauma, Stefanie Renee Pilkay

Doctoral Dissertations

Trauma and early-life stress have been linked to poor mental and physical health outcomes. In fact, research has identified trauma and stress can influence epigenetic marks on genes that can alter gene activity. It is suspected that epigenetically altered gene activity is involved in behavior and mental health. This may help explain why some individuals don’t experience great benefit from treatment for the effects of stress, and severe mental health symptoms can be chronic for decades or a lifetime. Moreover, some trauma-related mental health symptoms have shown generational patterns that appear linked to epigenetic marks. Therefore, this study sought ...


Morphological And Gene Expression Plasticity In Neotropical Cichlid Fishes, Sharon Fern Clemmensen Dec 2017

Morphological And Gene Expression Plasticity In Neotropical Cichlid Fishes, Sharon Fern Clemmensen

Doctoral Dissertations

Trophic divergence in cichlid fish is linked to morphological shifts in the pharyngeal jaw apparatus. For instance, in the Heroine cichlids of Central America, the ability to crush hard-shelled mollusks is a convergent phenotype with multiple evolutionary origins. These durophagous species often have very similar pharyngeal jaw morphologies associated with the pharyngeal jaw apparatus and some of these similarities could be due to phenotypically plastic responses to mechanical stress. I examined the durophagous cichlid Vieja maculicauda for differences in pharyngeal osteology, dentition, and soft tissues when exposed to different diet regimes. Here I discuss the effect on the morphology and ...


Investigating The Regulation Of Indole-3-Acetic Acid Production By The Plant Associated Microbe Pantoea Sp. Yr343, Kasey Noel Estenson Dec 2017

Investigating The Regulation Of Indole-3-Acetic Acid Production By The Plant Associated Microbe Pantoea Sp. Yr343, Kasey Noel Estenson

Doctoral Dissertations

The auxin indole-3-acetic acid (IAA) plays a central role in plant growth and development and many plant-associated microbes produce IAA. Several IAA biosynthetic pathways have been identified in microbes which use the precursor tryptophan. Pantoea sp. YR343, which was isolated from the Populus deltoides rhizosphere, is a robust plant root colonizer that produces IAA. Using genomic and metabolomics analyses, we predicted that the indole-3-pyruvate (IPA) pathway is the major pathway in Pantoea sp. YR343 for IAA production. To better understand IAA biosynthesis and the effects of IAA exposure on cell physiology, we performed proteomics on Pantoea sp. YR343 grown in ...


Non-Canonical Signaling From The Etr1 And Etr2 Ethylene Receptors In Arabidopsis Thaliana, Arkadipta Bakshi Dec 2017

Non-Canonical Signaling From The Etr1 And Etr2 Ethylene Receptors In Arabidopsis Thaliana, Arkadipta Bakshi

Doctoral Dissertations

The gaseous phytohormone ethylene regulates several physiological and developmental processes in higher plants. There are five ethylene receptor isoforms that mediate the responses to ethylene in the model plant Arabidopsis thaliana. Prior research has shown that these five ethylene receptor isoforms in Arabidopsis have both overlapping and non-overlapping roles in regulating diverse responses such as growth in air, growth recovery after removal of ethylene, and ethylene stimulated nutational bending. Functional divergence of ETR1 has been determined in controlling some of these traits and in some of these cases, ETR1 subfunctionalization requires the receiver domain. Using homology modeling and sequence alignment ...


Bioinformatic And Experimental Approaches For Deeper Metaproteomic Characterization Of Complex Environmental Samples, Ramsunder Mahadevan Iyer Dec 2017

Bioinformatic And Experimental Approaches For Deeper Metaproteomic Characterization Of Complex Environmental Samples, Ramsunder Mahadevan Iyer

Doctoral Dissertations

The coupling of high performance multi-dimensional liquid chromatography and tandem mass spectrometry for characterization of microbial proteins from complex environmental samples has paved the way for a new era in scientific discovery. The field of metaproteomics, which is the study of protein suite of all the organisms in a biological system, has taken a tremendous leap with the introduction of high-throughput proteomics. However, with corresponding increase in sample complexity, novel challenges have been raised with respect to efficient peptide separation via chromatography and bioinformatic analysis of the resulting high throughput data. In this dissertation, various aspects of metaproteomic characterization, including ...


Dissecting Molecular Pathways That Ensure Proper Chromosome Segregation And Cell Division, Anna Ye Nov 2017

Dissecting Molecular Pathways That Ensure Proper Chromosome Segregation And Cell Division, Anna Ye

Doctoral Dissertations

Equal segregation of the genome is a prerequisite for cell survival. During cell division the duplicated DNA is compacted into chromosomes and a multi-protein macrostructure, known as the kinetochore (Kt), is assembled on each copy of compacted DNA. Simultaneously, the mitotic spindle, which is made up of microtubules (MTs), is built to facilitate the equal distribution of the chromosomes between the resulting daughter cells. Kinetochores mediate the interaction between the MTs and the chromosomes, properly positioning them for segregation. To ensure that the DNA is equally divided in every cell division, cells have built a surveillance system to detect any ...


Golfer Exposure To Pesticides, Jeffery Doherty Nov 2017

Golfer Exposure To Pesticides, Jeffery Doherty

Doctoral Dissertations

There is concern among industry regulators, turfgrass managers, and the general public over human exposure to pesticides following their application to turfgrass. The residential aspect of turfgrass applications raises concerns about exposure to infants and children, as well as adults. To accurately determine the exposure of golfers to turfgrass pesticides, it is necessary to understand their transfer and absorption dynamics, as well as the routes of golfer exposure. Previous research has demonstrated that exposure to some turfgrass pesticides is at a level that would concern USEPA risk assessors using the USEPA Hazard Quotient (HQ) criteria (1). This current research used ...


Understanding Food Literacy And Its Use In A Technology-Driven Nutrition Education Program For Adolescents., Catherine A. Wickham Nov 2017

Understanding Food Literacy And Its Use In A Technology-Driven Nutrition Education Program For Adolescents., Catherine A. Wickham

Doctoral Dissertations

One in three adolescents in the U.S. is overweight or obese. The dietary habits of this population are concerning as few meet current dietary recommendations for consuming fruits and vegetables. Equally troubling among this group is the consumption of sugar-sweetened beverages and the lack of physical activity. Studies that investigate the link between nutrition knowledge, attitudes, and behaviors have shown mixed results and new methods to investigate this relationship are needed. Food literacy is a new term that has risen out of the health and nutrition literacy fields. Food literacy seeks to examine the complex relationship between knowledge, attitudes ...


Imidacloprid, A Neonicotinoid Insecticide, Impairs Lipid And Glucose Metabolism, Quancai Sun Nov 2017

Imidacloprid, A Neonicotinoid Insecticide, Impairs Lipid And Glucose Metabolism, Quancai Sun

Doctoral Dissertations

Emerging evidence suggests that environmental pollutants, including insecticides, are linked to excessive weight gain and altered glucose homeostasis. However, there is currently limited knowledge regarding the biochemical mechanisms by which exposure to insecticides contribute to obesity and its associated pathologies. We first studied the influence of fipronil (a phenylpyrazole family insecticide) on 3T3-L1 adipogenesis. Recently imidacloprid, a neonicotinoid insecticide, was reported to potentiate adipogenesis and insulin resistance in vitro. The current studies also determined the effects of imidacloprid exposure and its interaction with dietary fat in the development of adiposity and insulin resistance using both male and female C57BL/6J ...


Microbial Competition In Bioelectrochemical Systems, Varun Srinivasan Nov 2017

Microbial Competition In Bioelectrochemical Systems, Varun Srinivasan

Doctoral Dissertations

Bioelectrochemical systems(BESs)/ microbial fuel fells (MFCs) are a well-studied potential technology for bioremediation and decentralized wastewater treatment. However, progress has been somewhat stalled at the bench-scale. In well controlled experiments electron recovery is high. In natural environments, wastewaters are complex and anode-respiring bacteria can be outcompeted in the presence of competing microorganisms, leading to a loss in electron-recovery and power production. Furthermore, the cathode of the MFC plays a vital role in providing flexibility for treatment options but is an understudied part of MFCs.

Modelling Intracellular Competition in a Denitrifying Biocathode:

One potential MFC configuration uses an organic-oxidizing anode ...


Specialization And Trade-Offs In Plant-Feeding Insects, Daniel Peterson Nov 2017

Specialization And Trade-Offs In Plant-Feeding Insects, Daniel Peterson

Doctoral Dissertations

The immense diversity of life on Earth has been attributed to the partitioning of available resources into ecological niches, but it is not obvious what determines the niche size of each species. For example, most plant-feeding insects consume only one or a few closely-related host-plant species despite the advantages of having a broader diet. Many researchers have therefore suggested that the evolution of broad diets in plant-feeding insects must be constrained by genetic trade-offs between adaptations to alternative host-plants. Despite its intuitive feel, however, little empirical evidence in support of the trade-off hypothesis has emerged from decades of experimental studies ...


Protein-Nanoparticle Co-Engineering: Self-Assembly, Intracellular Protein Delivery, And Crispr/Cas9-Based Gene Editing, Rubul Mout Nov 2017

Protein-Nanoparticle Co-Engineering: Self-Assembly, Intracellular Protein Delivery, And Crispr/Cas9-Based Gene Editing, Rubul Mout

Doctoral Dissertations

Direct cytoplasmic delivery of gene editing nucleases such CRISPR/Cas9 systems and therapeutic proteins provides enormous opportunities in curing human genetic diseases, and assist research in basic cell biology. One approach to attain such a goal is through engineering nanotechnological tools to mimic naturally existing intra- and extracellular protein delivery/transport systems. Nature builds transport systems for proteins and other biomolecules through evolution-derived sophisticated molecular engineering. Inspired by such natural assemblies, I employed molecular engineering approaches to fabricate self-assembled nanostructures to use as intracellular protein delivery tools. Briefly, proteins and gold nanoparticles were co-engineered to carry complementary electrostatic recognition elements ...


Regulated Proteolysis Of Dnaa Coordinates Cell Growth With Stress Signals In Caulobacter Crescentus, Jing Liu Nov 2017

Regulated Proteolysis Of Dnaa Coordinates Cell Growth With Stress Signals In Caulobacter Crescentus, Jing Liu

Doctoral Dissertations

DNA replication is an essential process in all domains of life. Replication must be precisely regulated, especially at the step of initiation. In bacteria, the replication initiator DnaA is regulated by multiple post-translational regulations to ensure timely replication. Caulobacter crescentus has the most strict replication regulation that DNA only replicates once per cell cycle, and proteolysis of DnaA identified in this species is the only irreversible way to inhibit DnaA, suggesting it might be pivotal to restricting DNA replication. However, the responsible protease(s) and mechanism for its degradation remain unclear since its first discovery in 2005. In this thesis ...


Sex Differences In Estradiol Signaling In The Zebra Finch (Taeniopygia Gutatta) Auditory Cortex, Amanda Krentzel Nov 2017

Sex Differences In Estradiol Signaling In The Zebra Finch (Taeniopygia Gutatta) Auditory Cortex, Amanda Krentzel

Doctoral Dissertations

Although several sex differences have been described in brain structure, function, and development, sex as a biological factor is underrepresented in neuroscience studies. In the mammalian brain, there are sex differences in the mechanism of rapid estradiol actions on neuronal physiology. In the songbird, the brain is a major source of estradiol production, and estradiol rapidly modulates auditory responsiveness through dynamic changes and an unknown receptor mechanism. I set out to determine if there are sex differences in rapid estradiol modulation of auditory cortical activity, as has been shown in other systems. I tested this hypothesis through three aims: 1 ...


Physical Activity And Age-Related Mechanical Risk Factors For Knee Osteoarthritis, Jocelyn F. Hafer Nov 2017

Physical Activity And Age-Related Mechanical Risk Factors For Knee Osteoarthritis, Jocelyn F. Hafer

Doctoral Dissertations

Knee osteoarthritis is an age-related disease which will affect nearly 50% of individuals in their lifetime. Because there are currently no treatments to substantially slow the progression of this disease, it is important to identify mechanisms to reduce the risk of osteoarthritis initiation. Osteoarthritis is a disease which is at least partially mediated by mechanical factors which may result from age-related changes in gait. The extent to which habitual physical activity can modify the impact of age on gait, knee mechanics, and thus cartilage loading is unknown. The aim of this dissertation was to examine the effects of age and ...


A Comprehensive Validation Of Activity Trackers For Estimating Physical Activity And Sedentary Behavior In Free-Living Settings, Albert R. Mendoza Nov 2017

A Comprehensive Validation Of Activity Trackers For Estimating Physical Activity And Sedentary Behavior In Free-Living Settings, Albert R. Mendoza

Doctoral Dissertations

The aim of study one of this dissertation was to compare consumer activity trackers (ATs) with the research-grade ActiGraph™ GT3X-BT accelerometer (AG) in estimating energy expenditure (EE) and steps during orbital shaking at different frequencies. To address this aim, we utilized an electronic orbital shaking protocol (twenty-four, 3-minute trials; 2-hour trials). For all comparisons, the AG served as the reference measure. In the 3-min protocol, we showed that on average, the NL-1000 pedometer (NL) produced the lowest error (-9 steps/3-min) at 0.9 Hz (corresponding to moderate intensity). The magnitude of the error for the NL was 14 steps ...


Pore Forming Protein Assembly And The Use In Nanopore Sensing: A Study On E. Coli Proteins Clya And Ompg, Monifa Fahie Nov 2017

Pore Forming Protein Assembly And The Use In Nanopore Sensing: A Study On E. Coli Proteins Clya And Ompg, Monifa Fahie

Doctoral Dissertations

Pore forming proteins are typically the proteins that form channels in membranes. They have several roles ranging from molecule transport to triggering the death of a cell. This work focuses on two E. coli pore forming proteins that have vastly differing roles in nature. Outer membrane protein G (OmpG) is an innocuous β-barrel porin while Cytolysin A (ClyA) is an α-helical pore forming toxin. For OmpG we probed its potential to be a nanopore sensor for protein detection and quantification. A small high affinity ligand, biotin, was covalently attached to loop 6 of OmpG and used to capture biotin-binding proteins ...


Quantifying Gait Adaptability: Fractality, Complexity, And Stability During Asymmetric Walking, Scott W. Ducharme Nov 2017

Quantifying Gait Adaptability: Fractality, Complexity, And Stability During Asymmetric Walking, Scott W. Ducharme

Doctoral Dissertations

Successful walking necessitates modifying locomotor patterns when encountering organism, task, or environmental constraints. The structure of stride-to-stride variance (fractal dynamics) may represent the adaptive capacity of the locomotor system. To date, however, fractal dynamics have been assessed during unperturbed walking. Quantifying gait adaptability requires tasks that compel locomotor patterns to adapt. The purpose of this dissertation was to determine the potential relationship between fractal dynamics and gait adaptability. The studies presented herein represent a necessary endeavor to incorporate both an analysis of gait fractal dynamics and a task requiring adaptation of locomotor patterns. The adaptation task involved walking asymmetrically on ...


Microbial Dynamics And Design Considerations For Decentralized Microbial Fuel Cell Applications, Cynthia Castro Nov 2017

Microbial Dynamics And Design Considerations For Decentralized Microbial Fuel Cell Applications, Cynthia Castro

Doctoral Dissertations

The purpose of this dissertation was to assess the practicality of using microbial fuel cells (MFCs) as alternative sanitation systems for wastewater treatment and energy recovery, focusing on identifying key design considerations for treating high strength wastewater and managing alternative metabolic pathways.

We evaluated the energetic outputs of a lab-based pilot MFC designed to treat complex organics present in both synthetic feces and municipal wastewater. The pilot MFC produced two energetic products, methane and electricity, when treating two types of complex wastewaters. The energetic products associated with anode respiration and methanogenesis were simultaneously observed and yielded a combined energy ouput ...


Robust Biosensors For Healthcare Applications: From High-Content Screening To Point-Of-Care Testing, Ngoc D. B. Le Nov 2017

Robust Biosensors For Healthcare Applications: From High-Content Screening To Point-Of-Care Testing, Ngoc D. B. Le

Doctoral Dissertations

Efficient detection of proteins, mammalian cells, microorganisms and other biological systems in complex mixture is essential in disease diagnosis and environmental health. Therefore, technological platforms that provide sensors of high sensitivity, selectivity and stability are greatly desired. Recently, the ‘chemical-nose’ sensing approach has proved to be an effective strategy for profiling bio-relevant targets in complex mixtures. Detecting analytes in complex mixture is a challenge that conventional specificity-based sensors are still trying to solve due to the requirement of prior knowledge of the analyte, which is unknown in many cases. This thesis focuses on how to develop simple and robust ...


The Role Of Sleep On Inhibitory Control In Young Children With Attention-Deficit/Hyperactivity Disorder (Adhd), Amanda Cremone Nov 2017

The Role Of Sleep On Inhibitory Control In Young Children With Attention-Deficit/Hyperactivity Disorder (Adhd), Amanda Cremone

Doctoral Dissertations

Alongside the hallmark symptoms of hyperactivity and inattention, children with attention-deficit/hyperactivity disorder (ADHD) often report having sleep problems. Although sleep deficits are consistently found when evaluated subjectively, impairments in sleep physiology are inconsistent. Compared to typically developing (TD) children, children with ADHD have greater spectral power in the delta (0.5 to 4 Hz) and theta frequency bands (4 to 7 Hz). Moreover, activity in these bands is differentially related to cognitive outcomes in ADHD and TD populations. As such, this dissertation sought to examine relations between sleep physiology and inhibitory control, a primary deficit of ADHD, in young ...


Using The Suprachiasmatic Nucleus As A Model System To Assess Tolerance And Withdrawal To Alcohol, Jonathan Houghton Lindsay Aug 2017

Using The Suprachiasmatic Nucleus As A Model System To Assess Tolerance And Withdrawal To Alcohol, Jonathan Houghton Lindsay

Doctoral Dissertations

Alcohol abuse induces many disorders including depression, metabolic syndrome, and sleep disturbances. The strong link between alcohol abuse and sleep problems, along with the close connection between sleep and circadian rhythms, led us to investigate ethanol’s effects on the circadian clock. Previous work has shown that acute ethanol blocks photic phase shifts in vivo and glutamatergic phase shifts in vitro. However, neural systems become tolerant to ethanol across different timeframes. Despite both ethanol tolerance and ethanol withdrawal syndrome being listed as criteria for developing alcohol use disorders, little is known about how ethanol tolerance and withdrawal induced hyperexcitability develop ...


Effect Modification Of The Association Between Aerobic Physical Activity And Diabetes-Related Mortality By Race-Ethnicity: A Population-Based Prospective Study Using Nhanes Iii And 1999-2006 Nhanes, William Robert Boyer Ii Aug 2017

Effect Modification Of The Association Between Aerobic Physical Activity And Diabetes-Related Mortality By Race-Ethnicity: A Population-Based Prospective Study Using Nhanes Iii And 1999-2006 Nhanes, William Robert Boyer Ii

Doctoral Dissertations

Purpose: To examine potential effect modification by race-ethnicity of the relationship between physical activity (PA) and diabetes-related mortality risk using a sample of U.S. adults from the Third National Health and Nutrition Examination Survey (NHANES III). Methods: The sample (n=10,717) included adults (≥20 years) who attended the Mobile Examination Center (MEC). An age-standardized PA score (PAS) was calculated from the self-reported frequency and intensity of 12 leisure-time aerobic activities. The PA scores were then grouped into three categories: inactive (PAS = 0), insufficiently active (PAS >0 - Results: There was no interaction between PA and race-ethnicity (p=0.83 ...


Assessment Of Genetic And Education Recovery Plan Objectives For The Bog Turtle (Glyptemys Muhlenbergii), Cassie Marie Dresser Aug 2017

Assessment Of Genetic And Education Recovery Plan Objectives For The Bog Turtle (Glyptemys Muhlenbergii), Cassie Marie Dresser

Doctoral Dissertations

Unprecedented declines in biodiversity are threatening the natural world as we know it. Without human intervention, two thousand species listed under the US Endangered Species Act are likely to disappear. Fortunately, these species receive federal protection and increased research effort is needed to create and satisfy the objectives outlined in the mandated Species Recovery Plan. In this dissertation, I address three conservation objectives outlined in the Recovery Plan for North America’s smallest and rarest turtle, the Bog Turtle (Glyptemys muhlenbergii): (1) investigate the potential genetic differentiation in southern portions of the species’ range, (2) investigate the genetic impacts of ...


A Transcriptomic Analysis Of Salmonella Enterica Newport In Planta And After Postharvest Sanitization, Laurel Lynn Dunn Aug 2017

A Transcriptomic Analysis Of Salmonella Enterica Newport In Planta And After Postharvest Sanitization, Laurel Lynn Dunn

Doctoral Dissertations

The consumption of fresh produce is increasingly linked to incidence of foodborne illness. Pathogens including Salmonella enterica, Shiga toxigenic Escherichia coli, and Listeria monocytogenes are exposed to produce crops through direct human or animal contact, contaminated agricultural water, bioaerosols, run-off, and improperly treated compost. S. enterica has demonstrated exceptional tolerance to the stresses encountered in the environment, on plant tissue, and from postharvest antimicrobial mitigation strategies. Understanding the transcriptomic mechanisms S. enterica employs to survive these hazards is integral for the development of more effective preventive controls and post-harvest steps to prevent the pathogen from infecting consumers.


Genetic Analysis Of Field Populations Of The Plant Pathogens Cercospora Sojina, Corynespora Cassiicola And Phytophthora Colocasiae, Sandesh Kumar Shrestha Aug 2017

Genetic Analysis Of Field Populations Of The Plant Pathogens Cercospora Sojina, Corynespora Cassiicola And Phytophthora Colocasiae, Sandesh Kumar Shrestha

Doctoral Dissertations

Genetic markers and whole genome sequencing (WGS) were used to study the disease dynamics and population structure for three important plant pathogens; Cercospora sojina (frogeye leaf spot of soybean), Corynespora cassiicola (target spot of soybean, cotton and many other crops) and Phytophthora colocasiae (taro leaf blight). For each pathogen, genome sequencing was used to guide the development of single nucleotide polymorphism (SNP) markers and both were used to investigate diversity in field populations. Investigation of C. sojina in Tennessee included comparisons of extant populations and historical isolates, revealing a dominant, potentially long-lived, clonal lineage. Characterization of QoI fungicide resistance indicates ...


Transmission Pattern Of Major Clonal Lineages Of Toxoplasma Gondii, Pooja Saraf Aug 2017

Transmission Pattern Of Major Clonal Lineages Of Toxoplasma Gondii, Pooja Saraf

Doctoral Dissertations

Toxoplasma gondii is the most successful zoonotic pathogen known today. One-third of people are chronically infected worldwide. Different strains of T. gondii shows variability in mouse virulence which may potentially correlate with disease manifestation in humans. As a result, mouse is used as the model organism to study the virulence of T. gondii strains. To study the virulence on a global scale, it is necessary to establish a standardized approach for mice virulence assays. Thus, we review the methodologies used in different labs and put forth standardized approaches to study the T. gondii virulence in mice (Chapter 1). Recent advances ...