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Full-Text Articles in Life Sciences

The Efficacy Of Nanoemulsion-Based Delivery Systems To Improve Vitamin D3 Bioaccessibility And Bioavailability, Alagu Selvi Kadappan Jul 2019

The Efficacy Of Nanoemulsion-Based Delivery Systems To Improve Vitamin D3 Bioaccessibility And Bioavailability, Alagu Selvi Kadappan

Masters Theses

Vitamin D deficiency is an epidemic issue in all age groups in Western countries and that affects both skeletal and non-skeletal functions. Even with the wide application of food fortification, vitamin D deficiency tends to increase continuously. Being hydrophobic in nature, vitamin D has poor solubility; thereby it negatively affects its absorption and bioavailability when compared to other hydrophilic dietary compounds. The need to develop a novel strategy is of greater importance to enhance its bioavailability and thereby improving vitamin D level in the body.

In this study, lipid-based delivery of oil-in-water nanoemulsion (diameter < 200nm) was utilized to improve the bioaccessibility and oral bioavailability of vitamin D3. First, we examined the in ...


Use Of Alamarblue As An Indicator Of Microbial Growth In Turbid Solutions For Antimicrobial Evaluation, Precious Henshaw Oct 2018

Use Of Alamarblue As An Indicator Of Microbial Growth In Turbid Solutions For Antimicrobial Evaluation, Precious Henshaw

Masters Theses

The use of antibiotics in animal feed is a large cause of concern due to bacterial resistance, which occurs when bacteria change after exposure to antibiotics and become less affected by the drug. Consequently, the desire to find a replacement for these antibiotics has garnered interest in both farmers and consumers. The ideal substance would display antimicrobial activity without promoting bacterial resistance and would still function as a growth promoter in animals. One antimicrobial that fits this criterion is carvacrol, a compound found in oregano extract. Carvacrol exhibits antimicrobial activity in a number of bacteria, including E.coli O157:H7 ...


Use Of Different Ripening Inhibitors To Enhance Antimicrobial Activity Of Essential Oil Nanoemulsion, Victor Ryu Oct 2017

Use Of Different Ripening Inhibitors To Enhance Antimicrobial Activity Of Essential Oil Nanoemulsion, Victor Ryu

Masters Theses

The objective of this research was to study the impact of ripening inhibitor level and type on the formation, stability, and activity of antimicrobial thyme oil nanoemulsions formed by spontaneous emulsification. Oil-in-water antimicrobial nanoemulsions (10 wt%) were formed by titrating a mixture of essential oil, ripening inhibitor, and surfactant (Tween 80) into 5mM sodium citrate buffer (pH 3.5). Stable nanoemulsions containing small droplets (d < 70 nm) were formed. The antimicrobial activity of the nanoemulsions decreased with increasing ripening inhibitor concentration, which was attributed to a reduction in the amount of hydrophobic antimicrobial constituents transferred to the separated hydrophobic domain, mimicking bacterial cell membranes, by using dialysis and chromatography. The antimicrobial activity of the nanoemulsions also depended on the nature of the ripening inhibitor used: palm ≈ corn > canola > coconut which also depended on their ability to transfer hydrophobic antimicrobial constituents to the separated hydrophobic domain.


Novel Advancements For Improving Sprout Safety, Kyle S. Landry Jul 2016

Novel Advancements For Improving Sprout Safety, Kyle S. Landry

Doctoral Dissertations

All varieties of bean sprouts (mung bean, alfalfa, broccoli, and radish) are classified as a “super-food” and are common staples for health conscious consumers. Along with the proposed health benefits, there is also an inherent risk of foodborne illness. When sprouts are cooked, there is little risk of illness. The purpose of this dissertation was to explore novel techniques to minimize or prevent the incidence of foodborne illness associated with the consumption of sprouts. Three areas were investigated: 1) the use of a biocontrol organism, 2) the use of a novel spontaneous carvacrol nanoemulsion, and 3) the influence of the ...


Fish Oil Nanoemulsions: Optimization Of Physical And Chemical Stability For Food System Applications, Rebecca M. Walker Feb 2016

Fish Oil Nanoemulsions: Optimization Of Physical And Chemical Stability For Food System Applications, Rebecca M. Walker

Masters Theses

Emulsion-based delivery systems offer many potential benefits for incorporating omega-3 oils into foods and beverages. Nanoemulsions are emulsion-based delivery systems that are gaining popularity because of their ease of preparation, small particle size, relatively high stability, high bioavailability, and production of optically transparent emulsions. Fish oil (FO) nanoemulsions are potentially more susceptible to lipid oxidation because of their high degree of lipid unsaturation, high surface area of exposed lipids, and greater light penetration. In the first study, spontaneous emulsification, a low-energy method, was used to fabricate FO nanoemulsions. The influence of surfactant-to-oil-ratio (SOR) on particle size, turbidity, and physical stability ...


Development Of Nanoemulsion-Based Delivery Systems For Evaluation Of Triglycerides Bioactivity In Caernohabditis Elegans, Jose D. Colmenares Nov 2015

Development Of Nanoemulsion-Based Delivery Systems For Evaluation Of Triglycerides Bioactivity In Caernohabditis Elegans, Jose D. Colmenares

Masters Theses

Digestion and absorption of bioactive free fatty acids have been studied using the nematode Caernohabditis elegans (C. elegans). However, fatty acids mostly occur in foods in the form of triglycerides, which are highly hydrophobic molecules with low water-solubility, thereby making it difficult to study the fate of ingested fatty acids in C. elegans. The purpose of this research was to develop a method to deliver hydrophobic bioactives, including triglycerides, into C. elegans. Nanoemulsions containing triglyceride nanoparticles were prepared by sonication, and nanoparticle ingestion was confirmed by optical and confocal microscopy, and quantified by spectrometry. Changes in fatty acid composition were ...


Fabrication, Characterization And Biological Fate Of Phytochemical Delivery System, Jingjing Chen Nov 2015

Fabrication, Characterization And Biological Fate Of Phytochemical Delivery System, Jingjing Chen

Doctoral Dissertations

Polymethoxyflvones (PMFs) are a group compounds with promising cancer preventing activities and many other health benefits. There's a growing interest in fabricating delivery systems for PMFs as well as other phytochemicals due to their low water solubility. Firstly, we use nanoemulsion delivery system to encapsulate β-carotene. Sonication assisted method was developed to dissolve β-carotene to ensure minimum degradation. Powdered nanoemulsion was obtained after spray dry and freeze dry. Sample obtained after freeze dry showed better physiochemical characteristics. Then we use protein nanoparticle delivery system to encapsulate PMFS. The nanoparticle delivery system was fabricated by mixing the aqueous phase ...