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Full-Text Articles in Life Sciences

Understanding Lipid Oxidation In Low-Moisture Food, Leann M. Barden Nov 2014

Understanding Lipid Oxidation In Low-Moisture Food, Leann M. Barden

Doctoral Dissertations

Low-moisture snacks account for much of the saturated fat in the diet, making them a key target for improving consumers’ health. However, it is not currently feasible to maintain the same shelf life when replacing saturated fats with unsaturated fats in these products.

The first study characterized the microstructure of a low-moisture cracker model system and determined the impact of iron, chelators, and free fatty acids (FFA) on lipid oxidation kinetics. Confocal microscopy showed that lipids form a continuous layer surrounding the small and/or minimally gelatinized starch granules. Lipid-starch, lipid-air, and starch-lipid-protein interfaces all existed. Oxidation studies showed a ...


Sequence Analysis Of Maize Yellow Stripe3 Candidate Genes, Dennis B. Depaolo Nov 2014

Sequence Analysis Of Maize Yellow Stripe3 Candidate Genes, Dennis B. Depaolo

Masters Theses

The work presented here focuses on the molecular mechanism of phytosiderophore secretion in graminaceous plants. In maize, yellow stripe3 (ys3) is a mutant that is deficient in its ability to secrete iron-chelating compounds of the mugineic acid family known as phytosiderophores. Phytosiderophores are specific to grasses and are used for the acquisition of iron. Genetic linkage mapping of the ys3 locus lead to a region of interest on chromosome 3 defined by marker UMC1773. The sequence of eleven candidate genes (GRMZM2G390345, GRMZM2G390374, GRMZM2G342821, GRMZM5G800764, GRMZM2G502560, GRMZM5G849435, GRMZM2G105766, GRMZM5G876835, GRMZM2G036976, GRMZM2G502563, miR167g) revealed several small deletions and point mutations within the ...