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Full-Text Articles in Life Sciences

Engineering Surface Functionality Of Nanoparticles For Biological Applications, Yi-Cheun Yeh Nov 2014

Engineering Surface Functionality Of Nanoparticles For Biological Applications, Yi-Cheun Yeh

Doctoral Dissertations

Engineering the surface functionality of nanomaterials is the key to investigate the interactions between nanomaterials and biomolecules for potent biological applications such as therapy, imaging and diagnostics. My research has been orientted to engineer both of the surface monolayers and core materials to fabricate surface-functionalized nanomaterials through the synergistic multidisciplinary approach that combine organic chemistry, materials science and biology. This thesis illustrates the design and synthesis of the surface-funcitonalized quantum dots (QDs) and gold nanoparticles (AuNPs) for the fundamental studies and practical applications. For QDs, A new class of cationic QDs with quaternary ammonium derivatives was synthesized to provide permanent ...


Understanding The Transcriptional Regulation Of Secondary Cell Wall Biosynthesis In The Model Grass Brachypodium Distachyon, Pubudu Handakumbura Nov 2014

Understanding The Transcriptional Regulation Of Secondary Cell Wall Biosynthesis In The Model Grass Brachypodium Distachyon, Pubudu Handakumbura

Doctoral Dissertations

Secondary cell wall synthesis occurs in specialized cell types following completion of cell enlargement. By virtue of mechanical strength provided by a wall thickened with cellulose, hemicelluloses, and lignin, these cells can function as water-conducting vessels and provide structural support. Several transcription factor families regulate genes encoding wall synthesis enzymes. Certain NAC and MYB proteins directly bind upstream of structural genes and other transcription factors. The most detailed model of this regulatory network is established predominantly for a eudicot, Arabidopsis thaliana. In grasses, both the patterning and the composition of secondary cell walls are distinct from that of eudicots. These ...


Engineering Camelina Sativa For Biofuel Production Via Increasing Oil Yield And Tolerance To Abiotic Stresses, Kenny Ablordeppey Nov 2014

Engineering Camelina Sativa For Biofuel Production Via Increasing Oil Yield And Tolerance To Abiotic Stresses, Kenny Ablordeppey

Masters Theses

In an effort to engineer Camelina sativa for enhanced oil yield and tolerance to abiotic stresses, we have cloned and overexpressed Camelina γ-Glutamyl Cyclotransferase (GGCT2;1); a gene involved in oxidative stress tolerance via glutathione homeostasis and Wrinkled 1; a transcription factor that regulates genes involved in fatty acid biosynthesis to increase triacylglycerol (TAG) accumulation in seeds. The GGCT gene family in Camelina consists of three genes-GGCT1, GGCT2;1 and GGCT2;2. Camelina GGCT genes showed differential expression under oxidative stress caused as a result of exposure to various abiotic stresses. The GGCT2;1 gene, which showed strong up-regulation ...


Cd4 Silencing In Thymocytes Is Opposed By The Enforced Association Of P300 Hat, Hdac1 Or Suv39h1 With Runx Transcription Factors, Christyne Ayne Kane Apr 2014

Cd4 Silencing In Thymocytes Is Opposed By The Enforced Association Of P300 Hat, Hdac1 Or Suv39h1 With Runx Transcription Factors, Christyne Ayne Kane

Doctoral Dissertations

The transcription factors Runx1 and Runx3 are required for permanent silencing of CD4 in maturing CD8+ thymocytes. Runx binding to consensus sites within the CD4 silencer region is required for CD4 silencing post-positive selection. The Runx nuclear matrix targeting sequence (NMTS) is required for CD4 silencing and is implicated in binding to the histone acetyltransferase (HAT) p300, histone deacetylases (HDAC), and histone methyltransferase (HMT) SUV39H1 proteins. Epigenetic modifications of chromatin or post-translational modifications of Runx itself as a result of Runx association with these enzymes may be important for establishment of long-term CD4 silencing. In this study, we show that ...