Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 30 of 236

Full-Text Articles in Life Sciences

Cytochrome P450 Monooxygenase-Mediated Lipid Metabolism In Obesity And Colon Tumorigenesis, Weicang Wang Oct 2019

Cytochrome P450 Monooxygenase-Mediated Lipid Metabolism In Obesity And Colon Tumorigenesis, Weicang Wang

Doctoral Dissertations

Colon cancer is a major public health issue: it is expected to have 140,250 new cases and 50,630 deaths during 2018, making colon cancer the third most common type of cancer and the second leading cause of cancer death in the United States. Obesity is associated with enhanced colonic inflammation, which is a major risk factor of colorectal cancer. Currently, more than 35% of adults and nearly 17% of children are obese. Considering the obesity and colon cancer epidemic in the United States, there is an urgent need to identify novel therapeutic targets for obesity and colon cancer ...


Protein Detection And Structural Characterization By Mass Spectrometry Using Supramolecular Assemblies And Small Molecules, Bo Zhao Oct 2019

Protein Detection And Structural Characterization By Mass Spectrometry Using Supramolecular Assemblies And Small Molecules, Bo Zhao

Doctoral Dissertations

Mass spectrometry (MS) has played an increasingly prominent role in proteomics and structure biology because it shows superior capabilities in identification, quantification and structural characterization of proteins. To realize its full potential in protein analysis, significant progress has been made in developing innovative techniques and reagents that can couple to MS detection. This dissertation demonstrates the use of polymeric supramolecular assemblies for enhanced protein detection in complex biological mixtures by MS. An amphiphilic random co-polymer scaffold is developed to form functional supramolecular assemblies for protein/ peptide enrichment. The influences of charge density and functional group pKa on host-guest interactions ...


Factors Influencing Spartina Alterniflora Productivity In Relationship To Estuary Inlet Opening Ellisville Marsh, Plymouth, Ma, Ellen K. Russell Oct 2019

Factors Influencing Spartina Alterniflora Productivity In Relationship To Estuary Inlet Opening Ellisville Marsh, Plymouth, Ma, Ellen K. Russell

Doctoral Dissertations

A scientific basis for understanding the health of salt marsh vegetation, primarily Spartina alterniflora, in apposition to dredging of the tidal inlet to Ellisville Marsh in Plymouth, Massachusetts was determined. A three-hectare S. alterniflora loss, and coincident coastal bank erosion occurred; ostensibly due to inlet blockage from sand deposition, prolonged inundation, and meandering of inlet channel into coastal bank. This warranted dredging the tidal inlet, whereby tidal flushing would restore growth or discontinue die-off, and deter coastal bank erosion. A pre- and post dredge study was conducted assessing environmental factors related to plant productivity. Following dredging, an increase in tidal ...


Silver Mirror Substrate And Rolling Method For Improved Surface-Enhanced Raman Spesctroscopic Analysis In Food, Yanqi Qu Oct 2019

Silver Mirror Substrate And Rolling Method For Improved Surface-Enhanced Raman Spesctroscopic Analysis In Food, Yanqi Qu

Doctoral Dissertations

Surface-enhanced Raman spectroscopy, short for SERS, is an emerging technology with great potential in food analysis due to rapid detection, high sensitivity, portable instrumentation, and simple sample preparation. However, it is always a bottleneck to obtain reproducible SERS measurements in real analytical cases due to the complicity of food systems and inhomogeneous aggregation of colloidal nanoparticles. To improve its performance for practical applications in food analysis, efforts have been made in improving the reproducibility, enhancing the selectivity and reducing the matrix interference to the analyte. Herein, a self-assembly silver nanoparticles mirror substrate was fabricated to improve the and the quantitative ...


Drivers And Consequences Of Carbon Use Efficiency - And Its Measurement In Soil, Grace Pold Oct 2019

Drivers And Consequences Of Carbon Use Efficiency - And Its Measurement In Soil, Grace Pold

Doctoral Dissertations

Soils serve as massive carbon sinks, but their ability to continue this ecological service is contingent on how the resident soil microbial community will respond to the ongoing climate crisis. One key dimension of the microbial response to warming is its carbon use efficiency (CUE), or the fraction of carbon taken up by an organism which is allocated to growth rather than respiration. However, the scientific community is still in the early stages of understanding the drivers, consequences - and even accurate measurements of - CUE. In this dissertation, I first quantified the variability of CUE and its responsiveness to temperature and ...


Effects Of Fertilization And Drying Conditions On The Quality Of Selected Chinese Medicinal Plants, Zoe Gardner Oct 2019

Effects Of Fertilization And Drying Conditions On The Quality Of Selected Chinese Medicinal Plants, Zoe Gardner

Doctoral Dissertations

Demand for Chinese medicinal herbs in the U.S. is increasing and American acupuncturists and farmers are interested in domestic production of these plants. Little is known about the feasibility of production of these species outside of China. Four species of herbs were selected for cultivation trials. The purposes of this research were to evaluate the feasibility of cultivation of these species in the northeastern United States, to develop basic agronomic data for each species, and to determine the effects of nitrogen fertilizer on plant growth, yield, and secondary metabolites. Agastache rugosa, Schizonepeta tenuifolia, Leonurus japonicus, and Leonurus sibiricus were ...


Function And Dissipation In Finite State Automata - From Computing To Intelligence And Back, Natesh Ganesh Oct 2019

Function And Dissipation In Finite State Automata - From Computing To Intelligence And Back, Natesh Ganesh

Doctoral Dissertations

Society has benefited from the technological revolution and the tremendous growth in computing powered by Moore's law. However, we are fast approaching the ultimate physical limits in terms of both device sizes and the associated energy dissipation. It is important to characterize these limits in a physically grounded and implementation-agnostic manner, in order to capture the fundamental energy dissipation costs associated with performing computing operations with classical information in nano-scale quantum systems. It is also necessary to identify and understand the effect of quantum in-distinguishability, noise, and device variability on these dissipation limits. Identifying these parameters is crucial to ...


Improving Chinese Mothers’ Health Literacy: A Wechat Intervention, Qiong Chen Oct 2019

Improving Chinese Mothers’ Health Literacy: A Wechat Intervention, Qiong Chen

Doctoral Dissertations

The health literacy and eHealth literacy of women during the reproductive age is crucial, as it can affect their health and the health of their children. Promoting health literacy is essential to achieve mothers’ empowerment by improving access to and capacity of using health information effectively. However, functional, interactive, and critical health literacy and eHealth literacy have never been assessed among Chinese women.

The first study during this dissertation assessed functional, interactive, and critical health literacy and eHealth literacy among 421 of Chinese mothers with children under 3 years old. The results revealed overall less than optimal level of health ...


Multi-Level Barriers To Art Adherence Among Hiv-Infected Women In Rural Eswatini: A Mixed Methods Approach, Nozipho Becker Oct 2019

Multi-Level Barriers To Art Adherence Among Hiv-Infected Women In Rural Eswatini: A Mixed Methods Approach, Nozipho Becker

Doctoral Dissertations

Eswatini has the highest global prevalence of HIV despite universal access to free treatment. Lack of compliance continues to be a significant challenge for HIV care and management programs throughout the country. Studies investigating barriers to antiretroviral therapy (ART) adherence, particularly in women, who are the most vulnerable to HIV infections, are limited. The disproportionate impact of HIV on women can be attributed to multiple risk factors at the individual, household, and community/structural levels. Women living in rural settings are particularly at risk as a result of socio-cultural and environmental vulnerabilities such as oppressive traditional practices, intimate partner violence ...


Ecology Of The Elusive: Genome-Informed Investigation Of Soil Microbial Diversity, Lauren Alteio Oct 2019

Ecology Of The Elusive: Genome-Informed Investigation Of Soil Microbial Diversity, Lauren Alteio

Doctoral Dissertations

Soil is considered one of the most diverse ecosystems on Earth, harboring diversity of organisms across the three domains of life. It is spatially and chemically heterogeneous: properties that intertwine in a complex matrix to support organismal diversity and function across different scales. Soil microorganisms both respond to and drive changes in ecosystems through metabolic activities. A single gram of soil is teeming with millions of cells comprised of thousands of species. Much of this diversity remains uncharacterized due to technical and methodological challenges faced by soil ecologists. Due to the complex physicochemical properties of soil and cross-feeding interactions between ...


Determining Kinetic Parameters Of Thiamine Degradation In Three Nasa Spaceflight Foods In Thermal Processing And Long-Term Storage And Methods For Analyzing Microstructure And Precipitant Development In Real And Model Wines, Timothy R. Goulette Jul 2019

Determining Kinetic Parameters Of Thiamine Degradation In Three Nasa Spaceflight Foods In Thermal Processing And Long-Term Storage And Methods For Analyzing Microstructure And Precipitant Development In Real And Model Wines, Timothy R. Goulette

Doctoral Dissertations

The current NASA spaceflight food program encompasses a wide variety of commercial and custom-made edible products. These foods are designed to ensure each crewmember receives the Recommended Dietary Allowance (RDA) of vitamins and minerals to maintain adequate health during the entire spaceflight mission. With the desire for long-duration and exploration-class missions, such as the impending mission to Mars, the retention of important nutrients such as labile vitamins in spaceflight foods is critically important. For this reason, multiple studies were conducted to determine the vitamin degradation behavior of thiamine (vitamin B1) within three spaceflight foods which demonstrated both significant presence ...


Physical And Biological Drivers Of Juvenile Fish Distributions In Unstructured Shallow Tropical Nearshore Habitats, Christopher Haak Jul 2019

Physical And Biological Drivers Of Juvenile Fish Distributions In Unstructured Shallow Tropical Nearshore Habitats, Christopher Haak

Doctoral Dissertations

The structural complexity of aquatic habitats can influence the ecological processes that occur within them, as fine-scale topographic features act as refugia for small fishes, buffering the effects of environmental stressors. Accordingly, the habitat requirements of juvenile demersal fishes in shallow littoral zones are often defined by their associations with distinct benthic microhabitats, such as densely vegetated substrates. However, an array of ecologically-important juvenile fishes also associate with topographically-homogeneous, sparsely-vegetated substrata. Absent the benefits offered by structural refugia, such fishes may be more affected by environmental variability and may have evolved distinct strategies for coping with stressors. I examined this ...


Walking For Object Transport: An Examination Of The Coordinative Adaptations To Locomotor, Perceptual, And Manual Task Constraints, Avelino Amado Jul 2019

Walking For Object Transport: An Examination Of The Coordinative Adaptations To Locomotor, Perceptual, And Manual Task Constraints, Avelino Amado

Doctoral Dissertations

The goal of this dissertation was to understand how the intrinsic dynamics of gait adapt to support the performance of an ecologically relevant object transport task. A common object transport task is walking with a cup of water. Because the water can move relatively independent of the cup, the cup and water system is classified as a complex object. To model this task participants carried a cup with a wooden lid placed on top. On the lid there was a circular region with the same circumference as the cup and a ball. The object of the task was to keep ...


Metabolic Cost And Stability Of Locomotion In People With Lower Limb Amputation, Ryan Wedge Jul 2019

Metabolic Cost And Stability Of Locomotion In People With Lower Limb Amputation, Ryan Wedge

Doctoral Dissertations

It is generally accepted that metabolic energy expenditure and gait stability are key factors that influence the selection of able-bodied locomotor patterns. It is unclear how energy expenditure and gait stability are prioritized during walking in people with lower limb amputation. People with lower limb amputation generally have greater metabolic energy expenditure during walking and increased incidence of falls. People with unilateral lower limb amputation spend more time on the intact limb compared with the prosthetic limb, while able-bodied individuals generally walk with symmetrical timing between limbs. Restoring symmetry is often a goal of rehabilitation and assistive devices, yet the ...


Multiscale Simulations Of Intrinsically Disordered Proteins, Xiaorong Liu Jul 2019

Multiscale Simulations Of Intrinsically Disordered Proteins, Xiaorong Liu

Doctoral Dissertations

Intrinsically disordered proteins (IDPs) lack stable secondary and/or tertiary structures under physiological conditions. The have now been recognized to play important roles in numerous biological processes, particularly cellular signaling and regulation. Mutation of IDPs are frequently associated with human diseases, such as cancers and neuron degenerative diseases. Therefore, it is important to understand the structure, dynamics, and interactions of IDPs, so as to establish the mechanistic basis of how intrinsic disorder mediates versatile functions and how such mechanisms may fail in human diseases. However, the heterogeneous structural ensembles of IDPs are not amenable to high resolution characterization solely through ...


Acute Estrogen Synthesis And Action In The Auditory Cortex Of Developing Male Zebra Finches (Taeniopygia Guttata), Daniel Vahaba Jul 2019

Acute Estrogen Synthesis And Action In The Auditory Cortex Of Developing Male Zebra Finches (Taeniopygia Guttata), Daniel Vahaba

Doctoral Dissertations

Birdsong, as with human speech, is learned during an age- and experience-dependent sensitive period early in life. Songbirds must first memorize their parents’ song during a sensory phase, then refine their own burgeoning vocalizations to match the auditory memory of their parents’ song during a sensorimotor phase. While the error-correction aspect of the sensorimotor phase of song learning is comparatively well understood, it is largely unknown how auditory memories are formed and how auditory processing may change across development to facilitate song memorization. The songbird caudomedial nidopallium (NCM) is a brain region that encodes complex communication signals like song and ...


Feasibility And Efficacy Of A Recess-Based Combined Fitness Intervention On Cognition And Academic Performance In Elementary School Children, Christine St Laurent Jul 2019

Feasibility And Efficacy Of A Recess-Based Combined Fitness Intervention On Cognition And Academic Performance In Elementary School Children, Christine St Laurent

Doctoral Dissertations

Youth physical activity and fitness have been reported to influence cognition and academic related outcomes. Despite the potential benefits of muscular fitness, few intervention studies have examined the impact of an intervention that has incorporated both cardiorespiratory and muscular fitness training on cognition and academic performance in children. Previous studies have mainly been implemented either during the school day or immediately after-school. Although recess may be an ideal time to promote physical activity because it does not compete with other academic demands, it has been an understudied setting. Therefore, the purpose of this dissertation project was to examine the feasibility ...


The Spatial Organization Of Mycobacterial Membrane, Julia Puffal Jul 2019

The Spatial Organization Of Mycobacterial Membrane, Julia Puffal

Doctoral Dissertations

Mycobacteria comprises a large group of organisms including the pathogenic species Mycobacterium tuberculosis, the causative agent of tuberculosis. A fast- growing saprophytic member of this genus, however, Mycobacterium smegmatis, is oftentimes used as a model organism for the pathogenic species. With a unique cell envelope architecture and unconventional polar growth, spatial coordination of cell envelope biosynthesis is vital for proper assembly of this complex structure. Here, we provide a comprehensive overview of known lateral heterogeneities in mycobacterial plasma membrane, with a particular focus on the intracellular membrane domain (IMD), a spatially distinct region of the plasma membrane with diverse functions ...


Hybridized Polymeric Nano-Assemblies: Key Insights Into Addressing Mdr Infections, Ryan Landis Mar 2019

Hybridized Polymeric Nano-Assemblies: Key Insights Into Addressing Mdr Infections, Ryan Landis

Doctoral Dissertations

Multidrug-resistant (MDR) bacteria contribute to more than 700,000 annual deaths world-wide. Millions more suffer from limb amputations or face high healthcare treatment costs where prolonged and costly therapeutic regimens are used to counter MDR infections. While there is an international push to develop novel and more powerful antimicrobials to address the impending threat, one particularly interesting approach that has re-emerged are essential oils, phytochemical extracts derived from plant sources. While their antimicrobial activity demonstrates a promising avenue, their stability in aqueous media, limits their practical use in or on mammals. Inspired by the versatility of polymer nanotechnology and the ...


Population Viability And Connectivity Of The Federally Threatened Eastern Indigo Snake In Central Peninsular Florida, Javan Bauder Mar 2019

Population Viability And Connectivity Of The Federally Threatened Eastern Indigo Snake In Central Peninsular Florida, Javan Bauder

Doctoral Dissertations

Understanding the factors influencing the likelihood of persistence of real-world populations requires both an accurate understanding of the traits and behaviors of individuals within those populations (e.g., movement, habitat selection, survival, fecundity, dispersal) but also an understanding of how those traits and behaviors are influenced by landscape features. The federally threatened eastern indigo snake (EIS, Drymarchon couperi) has declined throughout its range primarily due to anthropogenically-induced habitat loss and fragmentation making spatially-explicit assessments of population viability and connectivity essential for understanding its current status and directing future conservation efforts.

The primary goal of my dissertation was to understand how ...


Small Fruit Phenolics: Phenolic Variations And Related Health Relevance, Susan Cheplick Mar 2019

Small Fruit Phenolics: Phenolic Variations And Related Health Relevance, Susan Cheplick

Doctoral Dissertations

Berries, specifically, strawberries, raspberries, blackberries, and blueberries, are considered an integral part of a healthy plant-based diet. Berries contain high levels of phenolic compounds, which contribute to the health benefits of berry consumption. Many phenolic compounds exhibit high levels of antioxidant activity and other health related benefits, including anti- microbial, anti-inflammatory and cardio-vascular related functional capabilities.

Strawberry and raspberry fruit were screened and evaluated for potential in dietary management of type 2 diabetes and related hypertension using in vitro assay models. There were differences between cultivars within each crop related to antioxidant activity. Inhibition of α- amylase, α-glucosidase, and ACE-1 ...


The Effects Of Social And Physical Interactions On Lizard Morphology, Behavior, And Ecology, Casey Gilman Mar 2019

The Effects Of Social And Physical Interactions On Lizard Morphology, Behavior, And Ecology, Casey Gilman

Doctoral Dissertations

Interactions with the physical and social aspects of an animal’s surroundings direct the trajectory of local adaptation and can lead to tremendous diversity within and across taxa. In my dissertation, I explored how interactions between lizards and their environment lead to morphological, behavioral, and ecological diversity. First, I examined how a common, but unexplored habitat characteristic, perch flexibility, affects jumping performance of an arboreal lizard. I found that in the lab, green anole lizards (Anolis carolinensis) did not take advantage of the natural recoil of the flexible perches, and suffered decreased jump distance and takeoff speed as a consequence ...


Nascent Dna Proteomics Analysis Uncovers Dna Replication Dynamics In The Human Pathogen Trypanosoma Brucei, Maria Rocha Granados Mar 2019

Nascent Dna Proteomics Analysis Uncovers Dna Replication Dynamics In The Human Pathogen Trypanosoma Brucei, Maria Rocha Granados

Doctoral Dissertations

DNA is the substrate of many cellular processes including DNA replication, transcription and chromatin remodeling. These processes are coordinated to maintain genome integrity and ensure accurate duplication of genetic and epigenetic information. Genome-wide studies have provided evidence of the relationship between transcription and DNA replication timing. A global analysis of DNA replication initiation in T. brucei showed that TbORC1 (subunit of the origin recognition complex, ORC) binding sites are located at the boundaries of transcription units. Although recent studies in T. brucei indicate functional links among DNA replication and transcription, the underlying mechanisms remain unknown. In this study, we adapted ...


Protein Degradation Regulates Phospholipid Biosynthetic Gene Expression In Saccharomyces Cerevisiae, Bryan Salas-Santiago Mar 2019

Protein Degradation Regulates Phospholipid Biosynthetic Gene Expression In Saccharomyces Cerevisiae, Bryan Salas-Santiago

Doctoral Dissertations

Transcriptional regulation of most phospholipid biosynthetic genes in Saccharomyces cerevisiae is coordinated by inositol and choline. Inositol affects phosphatidic acid (PA) intracellular levels. Opi1p interacts physically with PA and is the main repressor of the phospholipid biosynthetic genes. It is localized in the endoplasmic reticulum (ER) bound to the ER membrane protein Scs2p. When PA levels drop, Opi1p is translocated into the nucleus repressing most phospholipid biosynthetic genes. The OPI1 locus was identified in a screen looking for overproduction and excretion of inositol (Opi-). Opi- mutants are generally associated with a defect in repression of the ...


Chemoreceptor Dynamics And Signaling: Nmr Measurements Within Functional Complexes, Maryam Kashefi Mar 2019

Chemoreceptor Dynamics And Signaling: Nmr Measurements Within Functional Complexes, Maryam Kashefi

Doctoral Dissertations

Bacteria employ remarkable membrane-bound nanoarrays to sense their environment and direct their swimming. Arrays consist of chemotaxis receptor trimers of dimers that are bridged at their membrane-distal tips by rings of two cytoplasmic proteins, a kinase CheA and a coupling protein CheW. It is widely accepted that ligand binding to the receptor causes a 2 Å piston motion of a helix that extends through the periplasmic and transmembrane domains. However, it is not clear how the signal propagates 200 Å further to control activity of the kinase bound at the tip of the receptor. Dynamic changes within the cytoplasmic domain ...


Micronutrient Status And Telomere Length In Adult Men And Women., Christy S. Maxwell Nov 2018

Micronutrient Status And Telomere Length In Adult Men And Women., Christy S. Maxwell

Doctoral Dissertations

Background: Telomeres are the noncoding nucleotide sequences at the end of eukaryotic chromosomes, serving to protect DNA during mitotic division. Given their guanine-rich structure, telomeres are highly susceptible to oxidative damage often mediated by inflammation. Recently, telomere attrition and dysfunction have been associated with age-related diseases, including cardiovascular disease, type 2 diabetes, Alzheimer’s disease, and certain cancers. Several modifiable risk factors have also been associated with shortened telomere length, including physical inactivity, obesity, stress, smoking, and poor diet.

Objective: We examined the relations between vitamin B12 status, folate status, and iron status, relative to telomere length in eligible ...


Studies Of Dynein Anchoring Protein In Budding Yeast Saccharomyces Cerevisiae, Safia Omer Nov 2018

Studies Of Dynein Anchoring Protein In Budding Yeast Saccharomyces Cerevisiae, Safia Omer

Doctoral Dissertations

Inheritance of equal genomic content is vital for cellular growth and survival. During cell division, the mitotic spindle is built to ensure equal chromosome segregation between dividing cells. Fidelity of segregation is achieved by the precise positioning of the mitotic spindle by force generators located at the cell cortex where they exert pulling on microtubule (MT). Cortical dynein, a minus end directed motor, generates pulling forces via microtubule end-on capture-shrinkage and lateral MT sliding mechanisms. In Saccharomyces cerevisiae, the dynein attachment molecule Num1 interacts with plasma membrane (PM), endoplasmic reticulum (ER), and mitochondria to facilitate spindle positioning across the mother-bud ...


Cardiovascular Disease Risk And Menopause: Effects Of Cardiorespiratory Fitness, Exercise, And Follicle Stimulating Hormone, Corinna Serviente Nov 2018

Cardiovascular Disease Risk And Menopause: Effects Of Cardiorespiratory Fitness, Exercise, And Follicle Stimulating Hormone, Corinna Serviente

Doctoral Dissertations

Menopause is associated with adverse changes in cardiovascular disease risk factors. A reduction in estrogens is most commonly associated with changing cardiovascular disease risk; however, recent observations suggest that the increase in follicle stimulating hormone that accompanies menopause may also influence risk, potentially through its influence on lipid levels. The changes in cardiovascular disease risk factors may adversely affect endothelial cell function, a pre-clinical marker for cardiovascular disease. Whether cardiorespiratory fitness is protective of endothelial health in this population, thereby mitigating the changes in risk that accompany menopause, is unclear. This dissertation evaluated differences in endothelial health and endothelial responses ...


Epigenetic Regulation Of Genomic Imprinting During Early Mammalian Embryonic Development, Chelsea Marcho Nov 2018

Epigenetic Regulation Of Genomic Imprinting During Early Mammalian Embryonic Development, Chelsea Marcho

Doctoral Dissertations

Mammalian development involves remarkable changes, starting from a single-cell, totipotent zygote and ending with a developed organism comprised of diverse cells types with distinct morphologies, structures, and functions. Within three days of murine development, the two parental genomes merge into a single nucleus, begin zygotic gene expression, undergo epigenetic remodeling, and make the first lineage decisions. Diversity in cell-types is possible even though cells share the same genome. This diversity is achieved by the tight regulation of differential transcriptional programs. There are many ways these transcriptional programs can be initiated. Epigenetic alterations to the genome can drive transcriptional changes.

Epigenetic ...


Biotransformation Of Resveratrol And Its Implications In Biological Activities In The Colon, Fang Li Nov 2018

Biotransformation Of Resveratrol And Its Implications In Biological Activities In The Colon, Fang Li

Doctoral Dissertations

Resveratrol (RES) is a natural polyphenol compound with a wide range of health-promoting activities, including protective effects against colon cancer and renal disease. However, the premise of these benefits has been dampened since RES shows a poor oral bioavailability due to its rapid and extensive biotransformation after oral consumption. The paradox (low bioavailability but high bioactivity) warrants further investigations to determine the contribution of RES metabolites to the health benefits associated with RES. We identified 11 metabolites of RES in mice with high-resolution HPLC-MS/MS, then quantified two major metabolites - dihydro-resveratrol (DHR) and lunularin (LUN). To further understand the chemopreventative ...