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Full-Text Articles in Life Sciences

Investigating The Role Of Lbh During Early Embryonic Development In Xenopus Laevis, Emma Weir Oct 2019

Investigating The Role Of Lbh During Early Embryonic Development In Xenopus Laevis, Emma Weir

Masters Theses

LBH is a highly conserved protein whose role during vertebrate development is relatively under-studied. In collaboration with the Albertson lab, our lab has previously shown that it is necessary for cranial neural crest cell migration in the zebrafish and in Xenopus laevis. The molecular mechanisms through which it acts are not well understood.

In Xenopus, LBH is a maternally deposited protein. As such, studying its role in early development has not been feasible through the morpholino-mediated knockdown techniques that prevent translation of target genes. Recently, a technique for degrading endogenous proteins was developed, called Trim-Away. This was developed in mammalian ...


Patterns Of Morphological Plasticity In Metriaclima Zebra And Danio Rerio Suggest Differently Canalized Phenotypes Due To Form-Function Relationships, Dylan Jockel Oct 2019

Patterns Of Morphological Plasticity In Metriaclima Zebra And Danio Rerio Suggest Differently Canalized Phenotypes Due To Form-Function Relationships, Dylan Jockel

Masters Theses

In order to ascertain the degree of compatibility in developmental restructuring and behavioral plasticity between two fish species frequently made subject of laboratory research (Metriaclima zebra & Danio rerio), alternative trophic niche exposure experiments utilizing novel three-prong feeding treatments were conducted to obtain morphometric data, which demonstrated both species do bear some degree of plasticity. The results are somewhat complicated by differences in locality of detectable restructuring, which may be due to disparity in the form-function relationship for each species’ lineage. Each is notable in the manner of respective species’ jaw protrusion, as it is driven by anterior kinethmoid rotation in ...


Studies Of Dynein Anchoring Protein In Budding Yeast Saccharomyces Cerevisiae, Safia Omer Nov 2018

Studies Of Dynein Anchoring Protein In Budding Yeast Saccharomyces Cerevisiae, Safia Omer

Doctoral Dissertations

Inheritance of equal genomic content is vital for cellular growth and survival. During cell division, the mitotic spindle is built to ensure equal chromosome segregation between dividing cells. Fidelity of segregation is achieved by the precise positioning of the mitotic spindle by force generators located at the cell cortex where they exert pulling on microtubule (MT). Cortical dynein, a minus end directed motor, generates pulling forces via microtubule end-on capture-shrinkage and lateral MT sliding mechanisms. In Saccharomyces cerevisiae, the dynein attachment molecule Num1 interacts with plasma membrane (PM), endoplasmic reticulum (ER), and mitochondria to facilitate spindle positioning across the mother-bud ...


Epigenetic Regulation Of Genomic Imprinting During Early Mammalian Embryonic Development, Chelsea Marcho Nov 2018

Epigenetic Regulation Of Genomic Imprinting During Early Mammalian Embryonic Development, Chelsea Marcho

Doctoral Dissertations

Mammalian development involves remarkable changes, starting from a single-cell, totipotent zygote and ending with a developed organism comprised of diverse cells types with distinct morphologies, structures, and functions. Within three days of murine development, the two parental genomes merge into a single nucleus, begin zygotic gene expression, undergo epigenetic remodeling, and make the first lineage decisions. Diversity in cell-types is possible even though cells share the same genome. This diversity is achieved by the tight regulation of differential transcriptional programs. There are many ways these transcriptional programs can be initiated. Epigenetic alterations to the genome can drive transcriptional changes.

Epigenetic ...


Identifying Functional Components Of The Endoplasmic Reticulum Quality Control And Degradation Factor Edem1, Lydia Lamriben Nov 2018

Identifying Functional Components Of The Endoplasmic Reticulum Quality Control And Degradation Factor Edem1, Lydia Lamriben

Doctoral Dissertations

The ER Degradation-Enhancing Mannosidase-Like protein 1 (EDEM1) is a critical endoplasmic reticulum (ER) quality control factor involved in identifying and directing non-native proteins to the ER-associated protein degradation (ERAD) pathway. However, its recognition and binding properties have remained enigmatic since its discovery. Here we provide evidence for an additional redox-sensitive interaction between EDEM1 and Z/NHK that requires the presence of the single Cys on the α-1 antitrypsin ERAD clients. Moreover, this Cys-dependent interaction is necessary when the proteins are isolated under stringent detergent conditions, ones in which only strong covalent interactions can be sustained. This interaction is inherent to ...


Combination Regimens Using Dietary Components For The Chemoprevention Of Colorectal Cancer And Inflammation, Christina Dimarco-Crook Nov 2018

Combination Regimens Using Dietary Components For The Chemoprevention Of Colorectal Cancer And Inflammation, Christina Dimarco-Crook

Doctoral Dissertations

ABSTRACT

COMBINATION REGIMENS USING DIETARY COMPONENTS FOR THE

CHEMOPREVENTION OF COLORECTAL CANCER

AND INFLAMMATION

September 2018

CHRISTINA DIMARCO-CROOK, B.S., IMMACULATA UNIVERSITY

M.S., DREXEL UNIVERSITY

Ph.D., UNIVERSITY OF MASSACHUSETTS AMHERST

Directed by: Professor Hang Xiao

Dietary components have been found to effectively modulate multiple deregulated signaling pathways associated with the initiation and progression of carcinogenesis and inflammation in cellular and animal models. However, clinical studies have shown mixed results when examining the efficacy of individual dietary components, perhaps suggestive of the synergism that exists between multiple components within a particular food and the diet as a whole. Additional ...


The Interplay Between Polarity Regulators, Calcium, And The Actin Cytoskeleton During Tip Growth, Carlisle Bascom Jr Oct 2018

The Interplay Between Polarity Regulators, Calcium, And The Actin Cytoskeleton During Tip Growth, Carlisle Bascom Jr

Doctoral Dissertations

Plant cell growth is a meticulously regulated process whereby the cell wall is selectively loosened to allow for turgor-pressure driven expansion. The rate of expansion must equal delivery of new material, or the cell will lyse. In many plant cells, this process happens diffusely around the cell. However, a number of plant cells have anisotropic shapes that require exquisite spatial control of secretion. One simple example of anisotropic patterning is tip growth; highly polarized cell expansion utilized by pollen tubes, root hairs, and moss protonemata. Investigating the role various molecules have in tip growth sheds light on how plant cells ...


Influences Of Antroquinonol And 4-Acetylantroquinonol B On Inflammatory Tumorigenesis In The Mcf-7 Breast Cancer Cell Line With Or Without Tnf-Α Stimulation, Ting-Chun Lin Oct 2018

Influences Of Antroquinonol And 4-Acetylantroquinonol B On Inflammatory Tumorigenesis In The Mcf-7 Breast Cancer Cell Line With Or Without Tnf-Α Stimulation, Ting-Chun Lin

Masters Theses

Breast cancer (BC) is one of the most common cancers among women worldwide that ~25% of new cancer cases diagnosed every year would be BC; moreover, ~15% of cancer deaths per year caused by BC makes it the leading cause of cancer death among women worldwide. To date, though the cause of a large proportion of BC are still unclear, recent studies have revealed that a supportive breast tissue microenvironment is critical for the development and progression of BC, especially the communication with immune cells within breast tissue. Therefore, breast inflammatory microenvironment is currently received a substantial attention in the ...


Motor Protein Regulation In Mammalian Mitosis, Barbara Mann Jul 2018

Motor Protein Regulation In Mammalian Mitosis, Barbara Mann

Doctoral Dissertations

Developing and maintaining a multicellular organism relies on the fundamental biological process of cell division, which ensures that genetic material is equally segregated between daughter cells. During mitosis, cells completely rearrange their cytoskeleton into a bipolar spindle through the concerted efforts of microtubules, motor proteins, and microtubule-associated proteins, which cells must regulate spatially and temporally to prevent errors such as chromosomal missegregation: a major cause of cancer. Although the mitotic spindle is a validated target for chemotherapy drug resistance and redundant pathways have highlighted the need for new targets. It is therefore important to understand how proteins that help build ...


New Approaches In Engineering Somatic Embryogenesis In Loblolly Pine Suspension Cultures, Elizabeth Morgan Cummings Bende Jul 2018

New Approaches In Engineering Somatic Embryogenesis In Loblolly Pine Suspension Cultures, Elizabeth Morgan Cummings Bende

Doctoral Dissertations

Many industries including agriculture and healthcare require efficient methods for replication of plants with optimal traits. The loblolly pine (Pinus taeda) is a valuable crop in the timber industry, occupying 30 million acres of U.S. land, and breeding efforts aim to produce a crop with ideal phenotypic traits, including superior growth and wood quality. One method to large-scale clonal crop propagation is somatic embryogenesis (SE), the process through which asexual (somatic) plant cells undergo differentiation in vitro, resulting in germination-competent embryos. There are three main stages of growth and development that lead to the production of embryos: 1) aggregated ...


Identification Of The Mesoderm/Endoderm Interactions Involved In Murine Liver And Pancreas Development, Jesse Angelo Jul 2018

Identification Of The Mesoderm/Endoderm Interactions Involved In Murine Liver And Pancreas Development, Jesse Angelo

Doctoral Dissertations

The definitive endoderm, one of the three primary germ layers, arises during gastrulation as a simple epithelial sheet that will produce the entire gut tube and associated organs including the thyroid, lung, liver and pancreas. DiI fate mapping and whole embryo culture were used to determine the endodermal origin of the 9.5 days post coitum (dpc) dorsal and ventral pancreas buds. Our results demonstrate that the progenitors of each bud occupy distinct endodermal territories. Similarly, previous data has shown that the neighboring liver is formed from two separate progenitor populations. Numerous studies have demonstrated that signals secreted from adjacent ...


Clpxp Functions In Caulobacter As A Universal And Species-Specific Protease, Robert Vass Mar 2018

Clpxp Functions In Caulobacter As A Universal And Species-Specific Protease, Robert Vass

Doctoral Dissertations

Proteolysis shapes many aspects of cellular survival, including protein quality control and cellular signaling. Powered proteolysis couples ATP hydrolysis with a degradation force that actively probes and interrogates the protein population. ClpXP, exemplifies a conserved two-part protease system charged with powered proteolysis. This protease exists as a regulatory element (ClpX), and a compartmentalized, self-contained peptidase element (ClpP). In Caulobacter crescentus, ClpXP degradation plays a crucial role maintaining proteins that exhibit proper activity, and also triggers the start of cellular differentiation. Substrate elimination requires shared aspects of the protease from both quality control and precision protein destruction functions. Here, the regulatory ...


Mechanism Of Regulation Of Kinesins Eg5 And Kif15 By Tpx2, Sai Keshavan Balchand Mar 2018

Mechanism Of Regulation Of Kinesins Eg5 And Kif15 By Tpx2, Sai Keshavan Balchand

Doctoral Dissertations

Cell division is the fundamental process by which the replicated genetic material is faithfully segregated to form two identical daughter cells. The mitotic spindle is the macromolecular cytoskeletal structure that is built during every round of cell division to successfully separate the duplicated genome equally into the daughter cells. Errors in spindle formation can thus causegenetic aberrations and can potentially lead to cancer. Understanding the mechanisms that govern proper spindle assembly and function is thus important. Eg5 and Kif15 are two important kinesins which play a major role in establishing and maintaining bipolarity of the mitotic spindle. Both Eg5 and ...


Characterization Of Calcium Homeostasis Parameters In Trpv3 And Cav3.2 Double Null Mice, Aujan Mehregan Dec 2017

Characterization Of Calcium Homeostasis Parameters In Trpv3 And Cav3.2 Double Null Mice, Aujan Mehregan

Masters Theses

In mammals, calcium influx is required for oocyte maturation and egg activation, as it supports the persistent calcium oscillations induced by fertilization. These oscillations are required for the initiation of embryo development. The molecular identities of the plasma membrane calcium-permeant channels that underlie calcium influx are not established. Among these channels, Transient Receptor Potential Vanilloid, member 3 (TRPV3) allows divalent cations, namely strontium (Sr2+) and calcium (Ca2+) with high permeability, into cells, and its expression pattern seems to predict an essential role in the initiation of development. Another channel that was identified to be expressed in oocytes/eggs ...


Dissecting Molecular Pathways That Ensure Proper Chromosome Segregation And Cell Division, Anna Ye Nov 2017

Dissecting Molecular Pathways That Ensure Proper Chromosome Segregation And Cell Division, Anna Ye

Doctoral Dissertations

Equal segregation of the genome is a prerequisite for cell survival. During cell division the duplicated DNA is compacted into chromosomes and a multi-protein macrostructure, known as the kinetochore (Kt), is assembled on each copy of compacted DNA. Simultaneously, the mitotic spindle, which is made up of microtubules (MTs), is built to facilitate the equal distribution of the chromosomes between the resulting daughter cells. Kinetochores mediate the interaction between the MTs and the chromosomes, properly positioning them for segregation. To ensure that the DNA is equally divided in every cell division, cells have built a surveillance system to detect any ...


Protein-Nanoparticle Co-Engineering: Self-Assembly, Intracellular Protein Delivery, And Crispr/Cas9-Based Gene Editing, Rubul Mout Nov 2017

Protein-Nanoparticle Co-Engineering: Self-Assembly, Intracellular Protein Delivery, And Crispr/Cas9-Based Gene Editing, Rubul Mout

Doctoral Dissertations

Direct cytoplasmic delivery of gene editing nucleases such CRISPR/Cas9 systems and therapeutic proteins provides enormous opportunities in curing human genetic diseases, and assist research in basic cell biology. One approach to attain such a goal is through engineering nanotechnological tools to mimic naturally existing intra- and extracellular protein delivery/transport systems. Nature builds transport systems for proteins and other biomolecules through evolution-derived sophisticated molecular engineering. Inspired by such natural assemblies, I employed molecular engineering approaches to fabricate self-assembled nanostructures to use as intracellular protein delivery tools. Briefly, proteins and gold nanoparticles were co-engineered to carry complementary electrostatic recognition elements ...


Regulated Proteolysis Of Dnaa Coordinates Cell Growth With Stress Signals In Caulobacter Crescentus, Jing Liu Nov 2017

Regulated Proteolysis Of Dnaa Coordinates Cell Growth With Stress Signals In Caulobacter Crescentus, Jing Liu

Doctoral Dissertations

DNA replication is an essential process in all domains of life. Replication must be precisely regulated, especially at the step of initiation. In bacteria, the replication initiator DnaA is regulated by multiple post-translational regulations to ensure timely replication. Caulobacter crescentus has the most strict replication regulation that DNA only replicates once per cell cycle, and proteolysis of DnaA identified in this species is the only irreversible way to inhibit DnaA, suggesting it might be pivotal to restricting DNA replication. However, the responsible protease(s) and mechanism for its degradation remain unclear since its first discovery in 2005. In this thesis ...


Partial Craniofacial Cartilage Rescue In Ace/Fgf8 Mutants From Compensatory Signaling From The Ventricle Of Danio Rerio, Douglas A. Calenda Ii Oct 2017

Partial Craniofacial Cartilage Rescue In Ace/Fgf8 Mutants From Compensatory Signaling From The Ventricle Of Danio Rerio, Douglas A. Calenda Ii

Masters Theses

Examples of asymmetric organs are found throughout the animal kingdom. Whether it is superficial like the fiddler crab’s claw or within an organism like our visceral organs, asymmetries have repeatedly evolved in nature. However, the genetic and developmental origins for asymmetric organ development remain unclear, especially for superficially paired structures. Within zebrafish, a striking example of asymmetry occurs within the ace/fgf8 mutant. The pharyngeal cartilages of these mutants develop asymmetrically 35% of the time, with more cartilages developing on the left or right side of the head, but the origins of this asymmetry are unknown. A significant proportion ...


Methylglyoxal Influences Development Of Caenorhabditis Elegans Via Heterochronic Pathway, Jiaying Wang Jul 2017

Methylglyoxal Influences Development Of Caenorhabditis Elegans Via Heterochronic Pathway, Jiaying Wang

Masters Theses

Methylglyoxal is a highly reactive dicarbonyl compound, which is widely distributed in food products and beverages, and is particularly high in Manuka honey. In addition to its antibacterial effects, methylglyoxal is also known as a major precursor of advanced glycation end products (AGEs), that produces altered macromolecules (such as proteins and DNA), leading to abnormal physiological changes. However, the effects of methylglyoxal on development is unclear. Thus, this study aimed to determine the role of methylglyoxal in this aspect using Caenorhabditis elegans (C. elegans). Treatment of methylglyoxal at 0.1 mM and 1 mM for 48 h significantly inhibited development ...


The Key Question In Symbiotic Nitrogen Fixation: How Does Host Maintain A Bacterial Symbiont?, Onur Oztas Jul 2017

The Key Question In Symbiotic Nitrogen Fixation: How Does Host Maintain A Bacterial Symbiont?, Onur Oztas

Doctoral Dissertations

The fact that plants cannot use nitrogen in the gaseous form makes them dependent on the levels of usable nitrogen forms in the soil. Legumes overcome nitrogen limitation by entering a symbiotic association with rhizobia, soil bacteria that convert atmospheric nitrogen into usable ammonia. In root nodules, bacteria are internalized by host plant cells inside an intracellular compartment called the symbiosome where they morphologically differentiate into nitrogen-fixing forms by symbiosome-secreted host proteins.

In this project, I explained the host proteins required to maintain bacterial symbionts and described their delivery to the symbiosome. I showed that the SYNTAXIN 132 (SYP132) gene ...


An Adult Zebrafish Brain Atlas To Investigate Shh Mediated Cell-Cell Signaling In Neurogenic Zones, Alyssa P. Lutservitz Mar 2017

An Adult Zebrafish Brain Atlas To Investigate Shh Mediated Cell-Cell Signaling In Neurogenic Zones, Alyssa P. Lutservitz

Masters Theses

Adult neurogenesis occurs in proliferative zones of the brain that contain neural progenitor cell populations capable of differentiating into specific cell types. However, we remain limited in our understanding of the signals that regulate neural progenitor cell proliferation and differentiation in adults. Recently zebrafish (Danio rerio) have emerged as an excellent model for studying the molecular mechanisms behind adult neurogenesis, because sixteen proliferative zones remain active in the adult brains. Thousands of fluorescent transgenic reporter lines have been generated in zebrafish that reveal gene expression patterns of cell-cell signaling systems, some of which may regulate neurogenesis in these brain regions ...


The Far C-Terminus Of Tpx2 Contributes To Spindle Morphogenesis, Brett Estes Mar 2017

The Far C-Terminus Of Tpx2 Contributes To Spindle Morphogenesis, Brett Estes

Masters Theses

A cell must build a bipolar mitotic spindle in order to faithfully segregate replicated DNA. To do so, multiple microtubule nucleation pathways are utilized to generate the robust spindle apparatus. TPX2, a microtubule binding protein, holds crucial roles in both the Ran-dependent and Augmin-dependent pathways where microtubules are nucleated near the chromosomes and from pre-existing microtubules. However, the exact role TPX2 plays in branching microtubules is less understood. Here, we explored the effect of truncating the essential TPX2 C-terminal 37 amino acids on Augmin localization and branching microtubule activity. First, we depleted LLC-Pk1 cells of the Augmin subunit HAUS6 and ...


Thiol-Ene Chemistry As An Enabler Of New Polymer Structures And Architectures, Joel M. Sarapas Mar 2017

Thiol-Ene Chemistry As An Enabler Of New Polymer Structures And Architectures, Joel M. Sarapas

Doctoral Dissertations

This dissertation focuses on two distinct projects: the synthesis and design of novel cell penetrating peptides mimics (CPPMs), and the implementation of the thiol-ene click reaction to generate new polymer architectures and chemistries. Guanidinium-rich CPPMs were generated through both ROMP and RAFT polymerizations, allowing for a comparison to be made across polymer backbone chemistries with respect to both siRNA and protein cellular internalization. A particularly effective methacrylate derived block copolymer was able to deliver siRNA to nearly an entire Jurkat T cell population.

The thiol-ene reaction was implemented initially within the context of improving material design for solid polymer electrolytes ...


The Cellular Context Of Estradiol Regulation In The Zebra Finch Auditory Forebrain, Maaya Ikeda Nov 2016

The Cellular Context Of Estradiol Regulation In The Zebra Finch Auditory Forebrain, Maaya Ikeda

Doctoral Dissertations

Estradiol, traditionally known as a hormone that communicates with distant cells in the body, is also synthesized locally in the brain to act as a neuromodulator. Neuromodulators differ from neurotransmitters in that they simultaneously affect a population of neurons and their actions are not limited to the synapse. One of the many effects of estradiol signaling is rapid modulation of auditory processing in response to external stimuli. The enzyme required for estradiol synthesis, aromatase, is highly expressed in the regions that are involved in higher-order processing of sounds in humans and songbirds. Since zebra finches, a type of songbird, are ...


A Genetic Analysis Of Cichlid Scale Morphology, Kenta C. Kawasaki Nov 2016

A Genetic Analysis Of Cichlid Scale Morphology, Kenta C. Kawasaki

Masters Theses

Epidermal appendages are found on every vertebrate this world has to offer. In fish, these are commonly represented by scales. While we have a solid grasp of how scales develop, little is known about the underlying genetic mechanisms behind these phenotypic changes. Using two species of African cichlids (Labeotropheus fuelleborni and Tropheops “red cheek”) with varying scale phenotypes, we sought to examine their F2 hybrid offspring and statistically link the responsible genetic elements to their respective parental phenotypes through Quantitative Loci Trait (QTL) analysis.

Scales were removed from six different locations across the midline of each individual. Then, numerous traits ...


Inhibition Of Dna Methylation In Acquired Tamoxifen-Resistant Breast Cancer: Cell Line Model And Clinical Implications, Stephanie Zimmers Jul 2016

Inhibition Of Dna Methylation In Acquired Tamoxifen-Resistant Breast Cancer: Cell Line Model And Clinical Implications, Stephanie Zimmers

Doctoral Dissertations

One out of every eight American women will develop invasive breast cancer throughout their lifetime. Approximately 70% of breast cancers are estrogen receptor alpha (ER)-positive and can therefore be treated with an anti-estrogen such as tamoxifen. Although tamoxifen treatment has been successful at reducing breast cancer death rates, nearly one-third of women treated with tamoxifen for 5 years will have disease recurrence. Therefore, it is imperative that researchers investigate the mechanisms involved in developing acquired tamoxifen resistance and identify biomarkers that are predictive of acquired resistance.

DNA methylation is known to play a role in the development of breast ...


Alternative Methods For The Treatment Of Chemo-Resistant Cancers, Kaitlyn Wong Jul 2016

Alternative Methods For The Treatment Of Chemo-Resistant Cancers, Kaitlyn Wong

Doctoral Dissertations

Great strides have been made in cancer therapy in the past century, yet it remains one of the leading causes of death in the United States today. This work aimed to shed light on novel methods to treat a variety of aggressive and often chemo-resistant cancers both in vitro and in vivo.

The first aim of this work was to evaluate the therapeutic efficacy of poly(methacryloyloxyethyl phosphorylcholine) (polyMPC) prodrugs compared to standard chemotherapeutic agents. Conjugation of polyMPC to drugs such as doxorubicin (Dox) can result in its improved solubility, prolonged half-life and therapeutic efficacy. PolyMPC and polyMPC-Dox (at a ...


The Role Of The Formin Protein Family In Membrane Dynamics, Petrus Adrianus Cornelis Van Gisbergen Jul 2016

The Role Of The Formin Protein Family In Membrane Dynamics, Petrus Adrianus Cornelis Van Gisbergen

Doctoral Dissertations

Using molecular genetics, and high end imaging techniques, I assessed the function of the formin protein family in the moss Physcomitrella patens. Formins are proteins that can nucleate and elongate actin filaments. P. patens has 9 formins, divided over three classes. I found that a class II formin (For2A) is essential for polarized growth and specifically binds to the phosphoinositide PI(3,5)P2. Additionally, I show that this formin polymerizes actin filaments in vivo. I demonstrated that binding PI(3,5)P2 is essential for formin function.

My work also shows that one of the class I ...


Bioengineered Platforms To Study Carcinoma Cell Response To Drug Treatment, Thuy V. Nguyen Jul 2016

Bioengineered Platforms To Study Carcinoma Cell Response To Drug Treatment, Thuy V. Nguyen

Doctoral Dissertations

The tumor extracellular matrix (ECM) plays an important role in facilitating tumor growth and mediating tumor cells' resistance to drugs. However, during drug development, potential chemotherapeutics are screened in plastic plates, which lack relevant ECM physicochemical cues. In order to improve drug development process, this dissertation includes the development of relevant 2D and 3D biomaterial systems that can be used to study carcinoma cell response to drug treatment.

A novel poly(ethylene glycol)-phosphorylcholine (PEG-PC) high-throughput biomaterial platform was developed to study how the ECM mechanochemical properties affect cancer cells' response to drug. The PEG-PC biomaterial is optically transparent, has ...


An In Vivo Study Of Cortical Dynein Dynamics And Its Contribution To Microtubule Sliding In The Midzone, Heather M. Jordan Jul 2016

An In Vivo Study Of Cortical Dynein Dynamics And Its Contribution To Microtubule Sliding In The Midzone, Heather M. Jordan

Masters Theses

In LLC-Pk1 cells, and most cultured mammalian cells, cell division is highly regulated to achieve equal sized daughter cells. During this process, duplicated centrosomes separate and establish a bipolar array called the mitotic spindle. The mitotic spindle is responsible for aligning the chromosomes at the metaphase plate, and separating sister chromatids during anaphase. Spindle positioning and elongation are thought to be driven by the interaction between dynamic astral microtubules and cortical dynein. Extensive research has revealed that dynein is anchored to the cortex via the highly conserved NuMA/LGN/Gαi ternary complex in metaphase and the additional PIP/PIP2/NuMA ...