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Theses/Dissertations

California Polytechnic State University, San Luis Obispo

2015

E. coli

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

Demographics And Transfer Of Escherichia Coli Within Bos Taurus Populations, Joshua Ryan Dillard Sep 2015

Demographics And Transfer Of Escherichia Coli Within Bos Taurus Populations, Joshua Ryan Dillard

Master's Theses and Project Reports

In the United States, symptoms caused by pathogenic strains of Escherichia coli are on the rise. A major source of these pathogenic strains is the E. coli in the digestive tract of cattle. The purpose of this project was to determine if E. coli are transferred between individuals of the same species and if interspecies transmission is possible. Proximity of cattle was also studied as a contributing factor to the transfer of E. coli. To accomplish this goal, E. coli isolates from cattle and cohabitating ground squirrels were compared through a new method of bacterial strain typing called pyroprinting. Bulls ...


A Preliminary Report On Escherichia Coli Strain Diversity In Cows, Alison T. Stivers Mar 2015

A Preliminary Report On Escherichia Coli Strain Diversity In Cows, Alison T. Stivers

Biological Sciences

Pyroprinting is a strain typing method that relies on the simultaneous pyrosequencing of the multi-copy rRNA intergenic transcribed spacer regions of E. coli (Black, et al., 2014). These pyroprints can be used to identify the source of E. coli in the environment. Currently, Cal Poly’s Center for Applications in Biotechnology (CAB) is augmenting the existing E. coli pyroprint library. By pyroprinting the intestinal E. coli of cows, we can quantify the strain diversity present, evaluate persistence, and determine the minimum sample size required for a complete overview of the cow intestinal E. coli population. These pyroprints can then be ...