Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

PDF

Theses/Dissertations

California Polytechnic State University, San Luis Obispo

2015

Biological Sciences

Strain

Articles 1 - 1 of 1

Full-Text Articles in Life Sciences

A Preliminary Report On Escherichia Coli Strain Diversity In Cows, Alison T. Stivers Mar 2015

A Preliminary Report On Escherichia Coli Strain Diversity In Cows, Alison T. Stivers

Biological Sciences

Pyroprinting is a strain typing method that relies on the simultaneous pyrosequencing of the multi-copy rRNA intergenic transcribed spacer regions of E. coli (Black, et al., 2014). These pyroprints can be used to identify the source of E. coli in the environment. Currently, Cal Poly’s Center for Applications in Biotechnology (CAB) is augmenting the existing E. coli pyroprint library. By pyroprinting the intestinal E. coli of cows, we can quantify the strain diversity present, evaluate persistence, and determine the minimum sample size required for a complete overview of the cow intestinal E. coli population. These pyroprints can then be ...