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Full-Text Articles in Life Sciences

Development Of A Prolyl Endopeptidase Expression System In Lactobacillus Reuteri To Reduce The Clinical Manifestation Of Celiac Disease, Kara Lynn Jew Jul 2019

Development Of A Prolyl Endopeptidase Expression System In Lactobacillus Reuteri To Reduce The Clinical Manifestation Of Celiac Disease, Kara Lynn Jew

Master's Theses and Project Reports

Celiac Disease (CD) is an autoimmune disorder that emerges due to the ingestion of gluten, a protein found in a variety of common grains such as wheat, rye, and barley. Approximately 1 in 100 individuals in the US suffer from CD, making it the most commonly diagnosed gastrointestinal disorder (Ciclitira et. al., 2005). These proline-rich gluten peptides are resistant to proteolysis and accumulate in the duodenum of the small intestine. Once in the duodenum, these peptides illicit an autoimmune response resulting in villous atrophy. Current treatment for CD requires a rigorous adherence to a gluten-free diet. Nevertheless, gluten-containing grains are ...


Comparative Analysis Of Body Composition And Reference Values Of Visceral Adipose Tissues In Various American Collegiate Sports, Eshan M. Dandekar Sep 2018

Comparative Analysis Of Body Composition And Reference Values Of Visceral Adipose Tissues In Various American Collegiate Sports, Eshan M. Dandekar

Master's Theses and Project Reports

Background: Currently, body composition (BC) assessment is usually performed to diagnose disease states and accurately estimate certain types of tissues. In athletes, performing BC assessments helps gauge training and nutrition programs to see if they are adequately meeting the athlete’s needs to improve performance. Annual Dual Energy X-ray Absorptiometry (DXA) scans before the start of an athlete’s season can help identify an athlete’s preparedness or health before training begins.

Objective: To assess the preseason BC of four collegiate sports: Men’s basketball, men’s baseball, women’s volleyball, and women’s soccer, for Fat Mass (FM), bone ...


Detecting A Probiotic Product Within The Gut Of Broiler Chickens, Anneka Pisula Aug 2018

Detecting A Probiotic Product Within The Gut Of Broiler Chickens, Anneka Pisula

Master's Theses and Project Reports

As of January 2017, the U.S. poultry industry banned the use of antibiotics and now relies on alternatives such as probiotics to help protect animal health. Although probiotic use is not a new concept in the poultry industry, identifying the best combination of bacterial strains to generate an effective probiotic formula requires further investigation. This study aimed to detect a probiotic product of four bacterial strains (Pedioccoccus acidilactici, Pediococcus pentosaceus, Lactobacillus plantarum, and Bacillus subtilis) in a feeding trial with broiler chickens. Birds given the probiotic were predicted to show an improved growth performance with the probiotics colonizing the ...


Evaluation Of Endothelial Cell Responses To Elevated Glucose, Gabriella Sugerman Aug 2018

Evaluation Of Endothelial Cell Responses To Elevated Glucose, Gabriella Sugerman

Master's Theses and Project Reports

Developing a tissue-engineered Blood Vessel Mimic (BVM) to represent diabetic macrovascular disease could expedite design of new vascular devices specifically tailored to diabetic patients. In contribution toward this model, this thesis assessed Human Umbilical Vein Endothelial Cell (HUVEC) responses to high glucose conditions. Interleukin 6 (IL-6) and Cluster of Differentiation 36 (CD36) were selected to signify oxidative stress activity, a hallmark of diabetic macrovascular disease. Next, activity of potential reference genes B2M, HPRT1, and ACTB was assessed. All genes were found to exceed acceptable variability, so the E-ΔC T method of data analysis was selected. Next, cellular responses to high ...


Pink And Dude Chefs: Effectiveness Of An After-School Nutrition Knowledge And Culinary Skills Program For Middle School Students To Increase Fruit And Vegetable Consumption, Alyssa S. Vaziri Jun 2018

Pink And Dude Chefs: Effectiveness Of An After-School Nutrition Knowledge And Culinary Skills Program For Middle School Students To Increase Fruit And Vegetable Consumption, Alyssa S. Vaziri

Master's Theses and Project Reports

The rate of overweight and obesity among adolescents aged 12-19 years has more than tripled since 1980, and disproportionately impacts low-income and marginalized populations. Reduction in adolescent obesity rates may result in decreased health risks, decrease healthcare costs, and increased quality of life. Effective intervention methods for adolescent participants have incorporated nutrition knowledge and culinary skill building into afterschool programs. This study examines whether building knowledge, skills, and confidence through a culinary intervention can improve adolescent participants’ choices of healthful foods through increased fruit and vegetable intake.

Pink and Dude Chefs (PDC) is an afterschool nutrition education and culinary skills ...


Assessing The Effect Of Nutritional Interventions On Body Composition Changes During Pregnancy In Moderately Malnourished Malawian Women, Megan K. Kitagawa Sep 2017

Assessing The Effect Of Nutritional Interventions On Body Composition Changes During Pregnancy In Moderately Malnourished Malawian Women, Megan K. Kitagawa

Master's Theses and Project Reports

Background: Malnutrition can affect the body composition changes that occur in healthy pregnancy, resulting in adverse effects to both the mother and infant. Supplementary foods with high quality protein may result in more favorable changes in lean mass and fat mass in pregnant malnourished women.

Objective: To retrospectively assess changes in body composition in moderately malnourished pregnant Malawian women in response to one of three nutritional interventions.

Methods: Height, weight, MUAC, and TSF were measured in pregnant women with MUAC >20.6 cm and19) were compared using ANOVA. If differences were detected, differences between groups were tested using the Tukey ...


The Association Between Vitamin A Status Or Supplementation And Hiv/Aids, Mikaela Kathleen Curtis Mar 2016

The Association Between Vitamin A Status Or Supplementation And Hiv/Aids, Mikaela Kathleen Curtis

Food Science and Nutrition

The human immunodeficiency virus (HIV) has infected millions of people since the start of the epidemic in the early 1980s. HIV is the etiologic agent of the acquired immunodeficiency syndrome (AIDS), which has taken millions of lives. It is extremely difficult for particular populations to receive medication to treat HIV infection and prevent the progression of HIV to AIDS. Multiple studies have examined the effect of vitamin A on HIV infection, due to its immune stimulating effects. It is unclear whether an association exists between vitamin A and different aspects of HIV. This review examined the effect of vitamin A ...


Studies To Characterize Heavy Metal Content And Migration From Recycled Polyethyleneterephthalate, Michael John-Ross Whitt Dec 2014

Studies To Characterize Heavy Metal Content And Migration From Recycled Polyethyleneterephthalate, Michael John-Ross Whitt

Master's Theses and Project Reports

Packaging Materials account for 31% of the world’s municipal solid waste. Agencies like the Environmental Protection Agency (EPA) and the Agency for Toxic Substances and Disease Registry (ATSDR) are pushing for the increased use of recycled thermoplastic materials. Polyethylene terephthalate (PET) is a commonly recycled thermoplastic which is used to package ready-to-eat fruits and vegetables. Most recycled polyethylene terephthalate (RPET) packaging materials contain heavy metal catalysts, the most common being antimony. The recent increased use of recycled plastic materials has been suspected as the source of increased human heavy metal exposure. In this study, cadmium, chromium, nickel, lead and ...


A Comparison Of Fatigue During Cardiocerebral Resuscitation With Different Compression Rates Among Layperson And Professional Rescuers, Christopher Cassidy Aug 2014

A Comparison Of Fatigue During Cardiocerebral Resuscitation With Different Compression Rates Among Layperson And Professional Rescuers, Christopher Cassidy

Master's Theses and Project Reports

Quality chest compressions during Cardiopulmonary Resuscitation (CPR) are vital to maintaining adequate perfusion of oxygenated blood to the organs of the body to sustain life. Over the years, the compression rate recommended in Basic Life Support (BLS)/ Advanced Cardiac Life Support (ACLS) protocols for the best possible outcome has risen, and with that increase there are questions regarding rescuer fatigue and the effectiveness of compressions. Layperson and professional rescuers, answering to an emergency, both maintain continuous chest compressions until advanced life support arrives. Depending on the location, this arrival time would most likely be longer than the two minute standard ...


Incorporation Of Equine Assisted Therapy In University Curriculum, Shelby Y. Sabati Jun 2014

Incorporation Of Equine Assisted Therapy In University Curriculum, Shelby Y. Sabati

Animal Science

Equine-assisted activity and therapy incorporates physical, mental and language treatments through equine movement to remediate medical conditions or impairments in clients. For all the opportunities that equine-assisted therapy provides there is little education on the practice. The focus of this project is to present the process, benefits and limitations of adopting an equine-assisted therapy course at California Polytechnic State University so it may be used as a reference for future course proposal.

Objective: The aim of this proposal is to offer insight into Equine-assisted therapy, understanding how to integrate the Professional Association of Therapeutic Horsemanship (PATH) International curriculum into the ...


Characterization Of Corynebacterium Pseudotuberculosis Biovar Equi Isolates Obtained From Cal Poly Horses And Review Of Application Of Transposons To Better Prevent Pigeon Fever In Horses, Irene Hasen Jun 2014

Characterization Of Corynebacterium Pseudotuberculosis Biovar Equi Isolates Obtained From Cal Poly Horses And Review Of Application Of Transposons To Better Prevent Pigeon Fever In Horses, Irene Hasen

Animal Science

No abstract provided.


Developent Of A Phospholipid Encapsulation Process For Quantum Dots To Be Used In Biologic Applications, Logan Grimes Jun 2014

Developent Of A Phospholipid Encapsulation Process For Quantum Dots To Be Used In Biologic Applications, Logan Grimes

Master's Theses and Project Reports

The American Cancer Society predicts that 1,665,540 people will be diagnosed with cancer, and 585,720 people will die from cancer in 2014. One of the most common types of cancer in the United States is skin cancer. Melanoma alone is predicted to account for 10,000 of the cancer related deaths in 2014. As a highly mobile and aggressive form of cancer, melanoma is difficult to fight once it has metastasized through the body. Early detection in such varieties of cancer is critical in improving survival rates in afflicted patients. Present methods of detection rely on visual ...


Using Progesterone As An Indicator Of Ovarian Response To Stimulation In Cattle, Kayla Mariah Kauffman Mar 2014

Using Progesterone As An Indicator Of Ovarian Response To Stimulation In Cattle, Kayla Mariah Kauffman

Animal Science

No abstract provided.


The Effects Of 8 Weeks Of Low Dose Supplementation Of Creatine And Sodium Bicarbonate On Exercise Performance, Amanda Jessica Morris Dec 2013

The Effects Of 8 Weeks Of Low Dose Supplementation Of Creatine And Sodium Bicarbonate On Exercise Performance, Amanda Jessica Morris

Master's Theses and Project Reports

Short-term (3-7 days), high doses of creatine (20g/d) and/or sodium bicarbonate (0.5g/kg body weight) supplementation increase exercise performance during short term high intensity activities; however, it remains unclear whether long-term, low doses of these supplements have a positive impact on exercise performance. The purpose of this study was to determine the effects of long-term (8 weeks), low dose creatine supplementation on exercise performance, and whether combining creatine and sodium bicarbonate supplementation has an additive effect. Sixty-three healthy, habitually active, adults (28 M, 35 W; 22+2 years; 23+ 3 BMI) were randomly assigned by sex to ...


Urban Agriculture Stormwater Management In California Cities, Rachel L. Cohen Jun 2013

Urban Agriculture Stormwater Management In California Cities, Rachel L. Cohen

Master's Theses and Project Reports

Cities within California are beginning to incorporate urban agriculture into their land use designations. Prompted by residents and local organizations, cities are hoping to capture the benefits that urban agriculture provides. Research has shown that urban agriculture renews and beautifies neighborhoods, provides healthy food choices, increases public health, has the potential to help with stormwater runoff, creates jobs, and fosters community. In the last few years, several California cities have made headlines as they have adopted new zoning codes that include urban agriculture.

In reviewing these new zoning codes and exploring the topic of urban agriculture, it became evident that ...


Associations Between Alcohol Consumption And Fasting Blood Glucose In Young Adults, Julie Ann Lucca Jun 2013

Associations Between Alcohol Consumption And Fasting Blood Glucose In Young Adults, Julie Ann Lucca

Master's Theses and Project Reports

Current research shows moderate alcohol consumption is associated with decreased risk of diabetes and excessive consumption or binge drinking can cause insulin resistance and diabetes. In 2010, diabetes was the seventh leading cause of death in the United Statesand was responsible for significant health complications: blindness, kidney failure, and limb amputations, and is a large national economic burden. Fasting blood glucose (FBG) is a tool used to help diagnose diabetes. Abnormally high FBG, ≥100 mg/dl, is indicative of diabetes and pre-diabetes. Few studies have observed diabetic prevalence among young adults or college students. Studying young adults can help provide ...


Determinants Of Food Insecurity Among Vulnerable White And Latino Households: Contextualizing The Impact Of Sociodemographic And Household-Level Factors, Alexandra Lund May 2013

Determinants Of Food Insecurity Among Vulnerable White And Latino Households: Contextualizing The Impact Of Sociodemographic And Household-Level Factors, Alexandra Lund

Master's Theses and Project Reports

Household-level characteristics have been shown to be associated with food insecurity but studies among vulnerable populations are sparse. A food security assessment was developed to determine food security and collect sociodemographic and household level data across San Luis Obispo County. The assessments were administered to vulnerable groups through interviews at multiple sites across the County. Three household characteristics (marital status, number of children in the household and number of workers in the household) were examined in this analysis. A total of 808 surveys were collected, 69% in English and 31% in Spanish. Through ethnicity-stratified sequentially adjusted logistic regression models, the ...


Hypoxia-Sensitive Gene Expression In The Gastrocnemius Muscle Following Chronic Hind Limb Ischemia, Andrew Tilton Jul 2012

Hypoxia-Sensitive Gene Expression In The Gastrocnemius Muscle Following Chronic Hind Limb Ischemia, Andrew Tilton

Biological Sciences

Chronic ischemia, caused by the formation atherosclerotic plaque occlusions in major conduit arteries, is the leading cause of morbidity and mortality in western societies. Vascular remodeling can help compensate for the adverse effects of atherosclerotic plaque formation. Vascular remodeling relies heavily on vascular endothelial growth factor (VEGF), a critical protein that contributes to all forms of vascular formation and remodeling including angiogenesis, arteriogenesisand vasculogenesis. VEGF itself is up-regulated by the transcription factor, hypoxia inducible factor 1 alpha (HIF-1α), which becomes activated in low oxygen environments.

Through the use of animal chronic hindlimb ischemia models, these genes can be evaluated as ...


Development Of A Protocol To Measure Gene Expression In The Mouse Tibia, Daniel Hoover Jun 2012

Development Of A Protocol To Measure Gene Expression In The Mouse Tibia, Daniel Hoover

Biomedical Engineering

Numerous molecular factors active in bone tissue direct fracture repair and remodeling which can be altered by disease conditions such as Peripheral Arterial Disease (PAD) and Osteoporosis. Methods of molecular biology are commonly applied to investigate the expression and role of these molecular factors. This project presents a robust three-step protocol for examining gene expression in the mouse tibia. The protocol begins with isolating RNA from a flash frozen tibia sample. The isolated RNA is reverse transcribed into cDNA. Finally, PCR is performed to indentify expressed genes. Establishing this protocol will allow further research into the mechanisms of bone remodeling ...


Determining A Method For Rendering Low Cost Cdse(Zns) Core(Shell) Quantum Dots Aqueous Soluble Via Amphiphilic Polymer Wrapping, Patrick Mcbride Jun 2011

Determining A Method For Rendering Low Cost Cdse(Zns) Core(Shell) Quantum Dots Aqueous Soluble Via Amphiphilic Polymer Wrapping, Patrick Mcbride

Materials Engineering

Herein is described the procedure of two amphiphilic polymer wrapping techniques that may be employed for obtaining aqueous soluble quantum dots (QDs) for use in biological fluorescent imaging applications. The advent of QDs has led to new nanoscale fluorescent materials that exhibit unparalleled quantum yields (QYs), high resistance to photobleaching, tunable emissions, and
absorption over a large optical range. However, the QD synthesis employed here at Cal Poly to obtain bright, photostable CdSe(ZnS) core(shell) QDs involves the use of organic solvents and surfactants, leading to hydrophobic QDs. Since all of biology relies on aqueous solubility, this hydrophobicity creates ...


Ischemia Impairs Vasodilation In Skeletal Muscle Resistance Artery, Kyle Remington Struthers Jun 2011

Ischemia Impairs Vasodilation In Skeletal Muscle Resistance Artery, Kyle Remington Struthers

Master's Theses and Project Reports

Functional vasodilation in arterioles is impaired with chronic ischemia. We sought to examine the impact of chronic ischemia and age on skeletal muscle resistance artery function. To examine the impact of chronic ischemia, the femoral artery was resected from young (2-3mo) and adult (6-7mo) mice and the profunda femoris artery diameter was measured at rest and following gracilis muscle contraction 14 days later using intravital microscopy. Functional vasodilation was significantly impaired in ischemic mice (14.4±4.6% vs. 137.8±14.3%, p<0.0001 n=8) and non-ischemic adult mice (103.0±9.4% vs. 137.8±14.3%, p=0.05 n=10). In order to analyze the cellular mechanisms of the impairment, a protocol was developed to apply pharmacological agents to the experimental preparation while maintaining tissue homeostasis. Endothelial and smooth muscle dependent vasodilation were impaired with ischemia, 39.6 ± 13.6% vs. 80.5 ± 11.4% and 43.0 ± 11.7% vs. 85.1 ± 10.5%, respectively. From this data, it can be supported that smooth muscle dysfunction is the reason for the observed impairment in arterial vasodilation.


The Effects Of Carbohydrate, Caffeine, And Protein On Endurance Performance, Tony Zapata Mar 2011

The Effects Of Carbohydrate, Caffeine, And Protein On Endurance Performance, Tony Zapata

Kinesiology and Public Health

No abstract provided.


Angiostatin Like Peptides In Milk: Potential Development For Dairy Products Capable Of Cancer Prevention, Erin Stefanutti Mar 2011

Angiostatin Like Peptides In Milk: Potential Development For Dairy Products Capable Of Cancer Prevention, Erin Stefanutti

Master's Theses and Project Reports

For the past 40 years, antiangiogenic approaches have been of major interest in the development of methods to cure and prevent cancer. Angiogenesis, the development of blood vessels from pre-existing vascularization, is essential for cancer growth and spread of metastasis through the delivery of nutrients and oxygen essential to sustain the metabolic activity of these malignant cells. Blocking access to blood will cause cancerous cells to assume a dormant state creating inactive micro-tumors innocuous to the host. Angiostatin, the internal fragment of the fibrinolytic zymogen plasminogen, has shown great potential in reducing cancer size and number of metastatic colonies in ...


Cystic Ovaries In Dairy Cattle, Lacey Morgan Rosenberg Dec 2010

Cystic Ovaries In Dairy Cattle, Lacey Morgan Rosenberg

Dairy Science

The objective of this literature review was to determine the potential causes and factors affecting the incidence of ovarian cysts in dairy cattle as well as to examine functionality of various preventative methods and treatments of cysts. The end product was to be a document outlining all the information available on the three different types of cysts that can develop on the bovine ovary, follicular cysts, luteal cysts, and cystic corpora lutea. The literature that was reviewed, most in the forms of research papers, articles, and reviews, came primarily from scientific journals and published books. The literature reviewed was only ...


Development And Validation Of A Light Weight, Energy Dense, Ready To Eat (Rte) Bar, Jacob Wilhelm-Maria Heick Dec 2010

Development And Validation Of A Light Weight, Energy Dense, Ready To Eat (Rte) Bar, Jacob Wilhelm-Maria Heick

Master's Theses and Project Reports

DEVELOPMENT AND VALIDATION OF A LIGHT WEIGHT, ENERGY DENSE, READY TO EAT (RTE) BAR

Jacob Wilhelm-Maria Heick

Providing additional calories in the form of an RTE bar to endurance athletes will increase performance and muscle re-synthesis, reduce muscle breakdown, and shorten recovery time. An RTE bar containing a blend of dairy proteins and carbohydrates will create a product with superior functionality, including bioactive and immunity enhancing properties from dairy derived ingredients. The protein will provide benefits in the form of easily digestible calories, essential amino acids and physical satiate.

A formulation was developed and optimized, resulting in a final product ...


Trial And Budget Analysis Of Two Synchronized Artificial Insemination Breeding Programs For Beef First Calf Heifers, John Nino Jun 2010

Trial And Budget Analysis Of Two Synchronized Artificial Insemination Breeding Programs For Beef First Calf Heifers, John Nino

Agribusiness

This study was designed to compare the benefits and costs of two different synchronized artificial insemination programs on two groups of beef first calf heifers. A financial budget was developed that would include cost of synchronized insemination per animal and the added value of a bred heifer compared to an open one.

The two synchronized programs were called controlled internal drug release (CIDR) and Ovsynch. They used a specific timeline and drugs to complete the synchronization process. A single low birth weight high calving ease bull was used for both groups to enforce conformity between the groups. Costs of all ...


Group Feeding For The Lactating Cow, Anthony Joseph Martin Jan 2009

Group Feeding For The Lactating Cow, Anthony Joseph Martin

Dairy Science

Feeding individualized rations for lactating dairy cattle in the commercial herd based on dietary needs is a popular issue. This has been a debating topic since evolution of the Total Mixed Ration (TMR). Since dairymen were able to mix grain in a feed wagon and mix a specific dietary ration to meet the demand for an organized group, there has been an issue in which protocol cow pen grouping should take. Dairymen have always feed cattle based on if lactating or late in their gestation, nonlactating. With the evolution of the TMR dairymen are then allowed to go into more ...