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Theses/Dissertations

California Polytechnic State University, San Luis Obispo

Animal Sciences

Embryo

Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

Metabolic Regulation And Cryotolerance Of In Vitro-Produced Holstein Embryos, Melissa Ann Roberts Dec 2016

Metabolic Regulation And Cryotolerance Of In Vitro-Produced Holstein Embryos, Melissa Ann Roberts

Master's Theses and Project Reports

In vitro production and transfer of embryos has become a common practice within the dairy industry to efficiently breed superior animals and meet the consumption demand of the growing population. Cyropreservation is necessary for the application of commercialized embryo transfer, however, in vitro-produced embryos show morphological and physiological defects which negatively impact their ability to withstand cryopreservation in comparison to their in vivo counterparts. These artifacts result from culture conditions that cause stress to the embryo during development, leading to an accumulation of intracellular lipids, mitochondrial dysfunction, and ultimately poor ability to withstand freezing and thawing. The objective of ...


The Effects Of Different Cryopreservation Techniques On The Survival Rate Of In Vitro Produced (Ivp) And Biopsied Bovine Blastocysts, Diana J. Gumber Jun 2014

The Effects Of Different Cryopreservation Techniques On The Survival Rate Of In Vitro Produced (Ivp) And Biopsied Bovine Blastocysts, Diana J. Gumber

Animal Science

This study’s objectives were to determine if the biopsy of expanded blastocysts prior to cryopreservation affected the survival rates and determine if there is a difference between slow-freezing in 10% glycerol, slow freezing in ethylene glycol, and vitrification in terms of survival rate of the embryos. Blastocoeles were reduced prior to cryopreservation by means of biopsy. Embryos for slow freezing were exposed to ethylene glycol (SynGRO Ethylene Glycol with Sucrose, BIONICHE, Pullman, WA, USA) or 10% glycerol (SynGRO 10% Glycerol, BIONICHE, Pullman, WA, USA) as cryoprotectants. Vitrified embryos were exposed to 10% and 40% ethylene glycol. Survival rate of ...


Efficiency Of Two Cryopreservation Methods Using Direct In-Straw Rehydration After Repeated Vitrification Of Mouse Embryos, Ashley Morgan Payne Jun 2012

Efficiency Of Two Cryopreservation Methods Using Direct In-Straw Rehydration After Repeated Vitrification Of Mouse Embryos, Ashley Morgan Payne

Animal Science

Experiments were conducted to determine which of two direct in-straw rehydration methods was optimal for obtaining high survival of mouse embryos after repeated vitrification. The first vitrification method compared was a one-step design, designated “House method,” where embryos were equilibrated in 3.5 M ethylene glycol for 3 min before rapid plunge vitrification in 7 M ethylene glycol/0.5 M glucose/18% w/v Ficoll 70. The second method was the commercially available BoviPRO Embryo Vitrification Kit TM(MiniTube of America, Verona, WI), which first exposed embryos to 1.4 M glycerol for 5 min, then 1.4 M ...