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Full-Text Articles in Life Sciences

The Effects Of Acidosis On Calcium Dependent Binding Of A Single Crossbridge, Matthew Unger Oct 2019

The Effects Of Acidosis On Calcium Dependent Binding Of A Single Crossbridge, Matthew Unger

Masters Theses

Intracellular acidosis is a putative agent of skeletal muscle fatigue, in part, because acidosis depresses the calcium (Ca2+) sensitivity and force production of muscle (18, 50). However, the molecular mechanisms behind this depression in Ca2+ sensitivity and force production are unknown. This gap in knowledge poses a significant challenge in generating a complete understanding of the fatigue process. To close this gap, the ability of myosin to bind to a single actin filament was measured under acidic conditions, in a laser trap assay, with and without regulatory proteins. Decreasing pH from 7.4 to 6.5 reduced the ...


Analysis Of Connectivity In Emg Signals To Examine Neural Correlations In Muscular Activation Of Lower Leg Muscles For Postural Stability, Diana Mccrumb Oct 2019

Analysis Of Connectivity In Emg Signals To Examine Neural Correlations In Muscular Activation Of Lower Leg Muscles For Postural Stability, Diana Mccrumb

Masters Theses

In quiet standing the central nervous systems implements a pre-programmed ankle strategy of postural control to maintain upright balance and stability. This strategy is comprised of a synchronized common neural drive being delivered to synergistically grouped muscles. In this study connectivity between EMG signals of unilateral and bilateral homologous muscle pairs, of the lower legs, during various standing balance conditions was evaluated using magnitude squared coherence (MSC) and mutual information (MI). The leg muscles of interest were the tibialis anterior (TA), medial gastrocnemius (MG), and the soleus (S) of both legs. MSC is a linear measure of the phase relation ...


Tropomyosin-Based Effects Of Acidosis On Thin-Filament Regulation During Muscle Fatigue, Brent Scott Jul 2019

Tropomyosin-Based Effects Of Acidosis On Thin-Filament Regulation During Muscle Fatigue, Brent Scott

Masters Theses

Skeletal muscle fatigue is defined as a loss in the force/velocity generating capacity of a muscle. A portion of the loss in function is attributable to effects of acidosis (i.e. low pH) on the regulatory proteins, troponin and tropomyosin (Tm), which regulate the binding of myosin and actin in a calcium (Ca++) dependent manner. However, the relative role of troponin and Tm on myosin-actin function during acidosis is not clear, nor are the mechanisms underlying these effects. PURPOSE: To determine the role of Tm in the acidosis-induced depression of muscle function using isolated muscle proteins in an in ...


Development And Cross-Validation Of A Cadence-Based Metabolic Equation For Walking, Christopher C. Moore Jul 2019

Mechanisms And Mitigation Of Skeletal Muscle Fatigue In Single Fibers From Older Adults, Aurora Foster Jul 2019

Mechanisms And Mitigation Of Skeletal Muscle Fatigue In Single Fibers From Older Adults, Aurora Foster

Masters Theses

Skeletal muscle fatigue is the contraction-induced decline in whole muscle force or power, and can be greater in older versus young adults. Fatigue primarily results from increased metabolism elevating phosphate (Pi) and hydrogen (H+), which alters myosin-actin interactions; however, which steps of the myosin-actin cross-bridge cycle are changed and their reversibility are unclear. PURPOSE: This study sought to: 1) Examine the effects of elevated Pi and H+ on molecular and cellular function, and 2) Test the ability of deoxyadenosine triphosphate (dATP), an alternative energy to adenosine triphosphate (ATP), to reverse the contractile changes induced with high Pi ...


Waist Circumference And The Relation To Aerobic Exercise And Perception Of Illness In Adults With Type 2 Diabetes Mellitus, Bailey J. Huebner Jan 2019

Waist Circumference And The Relation To Aerobic Exercise And Perception Of Illness In Adults With Type 2 Diabetes Mellitus, Bailey J. Huebner

Masters Theses

The purpose of this study was to observe the relationship between waist circumference (WC), amount of aerobic exercise performed weekly and overall Health Belief Model (HBM) score in adults with diagnosed type 2 diabetes mellitus (T2DM). Members from a local Midwest adult fitness program (n = 5) who had been diagnosed T2DM participated in this study. Participants ranged in age from 70 to 80 years with an average age of 74 years. Four of the participants were female and one participant was male. The questions included in this study were 1 .) in those with diagnosed T2DM, was a low overall HBM ...