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Full-Text Articles in Life Sciences

Generation, Isolation And Assay Methods For Human Lymphocyte Mitogenic Factor, Thomas E. Seay Dec 1982

Generation, Isolation And Assay Methods For Human Lymphocyte Mitogenic Factor, Thomas E. Seay

Electronic Theses and Dissertations

Activated lymphocytes secrete many products including the lymphokine human lymphocyte mitogenic factor (HLMF). In preliminary experiments lymphocytes from peripheral blood and palatine tonsils were evaluated as possible sources of HLMF by evaluating their level of activation through screening their spontaneous and concanavalin A (con A)-induced blastogenic responses. Tonsil lymphocytes (TL) were found to have high spontaneous proliferation as compared to peripheral blood lymphocytes (PBL). Cells from both sources responded to con A by undergoing a typical blastogenic response. Because TL must be obtained septically, they are frequently cultured in the presence of the antimycotic agent, Amphotericin B (Am B ...


Tonsil Cell Products Which Modify In Vitro Proliferation Of Blood Lymphocytes, Thomas W. Hodge May 1982

Tonsil Cell Products Which Modify In Vitro Proliferation Of Blood Lymphocytes, Thomas W. Hodge

Electronic Theses and Dissertations

Human palatine tonsil lymphocytes, when compared to peripheral blood lymphocytes (PBL), were in an activated state even though there was no in vitro stimulation. When these tonsil lymphocytes were cultured in the absence of serum and polyclonal mitogens or antigens, the supernatant fluid often inhibited the proliferative response of target PBL to con A. The extent of this suppression ranged from 22% to 84%, and target cell viability was 90% or greater. There was no evidence for the presence of immunoglobulins or (alpha)2-macroglobulin in whole supernatant fluids. The suppressor was partially denatured at 80(DEGREES)C and was rendered ...


Recombinants Of The Major Histocompatibility Complex In The Chicken , Douglas Keith Palmer Jan 1982

Recombinants Of The Major Histocompatibility Complex In The Chicken , Douglas Keith Palmer

Retrospective Theses and Dissertations

The primary purpose of this research was to produce and analyze recombinants of the major histocompatibility complex of the chicken in order to provide useful information concerning its structure and function. Recombinant research provides data relating to the linear order of genes and aids in elucidating gene functions. On a practical level, such knowledge bears on the health and well-being of a species. From the point of view of evolutionary origin, this information complements that already known of the mammalian major histocompatibility complex;From matings of B complex heterozygotes (B('1)B('19)) to homozygotes (B('2)B('2)), 5 ...