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Theses/Dissertations

Food Science

Doctoral Dissertations

Electrospinning

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Biopolymer Electrospun Nanofiber Mats To Inactivate And Remove Bacteria, Katrina Ann Rieger Nov 2016

Biopolymer Electrospun Nanofiber Mats To Inactivate And Remove Bacteria, Katrina Ann Rieger

Doctoral Dissertations

The persistence of antibiotic resistance in bacterial pathogens remains a primary concern for immunocompromised and critically-ill hospital patients. Hospital associated infections can be deadly and reduce the successes of medical advancements, such as, cancer therapies and medical implants. Thus, it is imperative to develop materials that can (i) deliver new antibiotics with accuracy, as well as (ii) uptake pathogenic microbes. In this work, we will demonstrate that electrospun nanofiber mats offer a promising platform for both of these objectives because of their high surface-to-volume ratio, interconnected high porosity, gas permeability, and ability to contour to virtually any surface. To provide ...


Functionalized Electrospun Nanofibers For Food Science Applications, Minhui Dai Jul 2016

Functionalized Electrospun Nanofibers For Food Science Applications, Minhui Dai

Doctoral Dissertations

This work is separated into two individual parts. The first part demonstrates the ability to electrospin reagents into water-soluble nanofibers resulting in a stable on-chip enzyme and a microphage storage format.

1a) Polyvinylpyrrolidone (PVP) nanofibers were spun incorporating the enzyme horseradish peroxidase (HRP). Scanning electron microscopy of the spun nanofibers was used to confirm the non-woven structure with an average diameter of 155±34 nm. The HRP containing fibers were tested for a change in activity following electrospinning and during storage. A colorimetric assay was used to characterize the activity of HRP reacting with the nanofiber mats in a microtiter ...


Enzyme Stabilization In Hierarchical Biocatalytic Food Packaging And Processing Materials, Dana Erin Wong Jul 2016

Enzyme Stabilization In Hierarchical Biocatalytic Food Packaging And Processing Materials, Dana Erin Wong

Doctoral Dissertations

The partnership of biocatalysts and solid support materials provides many opportunities for bioactive packaging and bioprocessing aids beneficial to the agricultural and food industries. Biocatalysis, or reactions modulated by enzymes, allows bioactive materials to assist in bringing a substrate to product. Enzymes are proteins which catalyze reactions by lowering the activation energy required to drive the production of a desired product. Enzymes are commonly utilized in food processing as catalysts with specificity in order to enhance product quality through the production of beneficial food components, and to break down undesirable components that may be harmful or may decrease product quality ...