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Theses/Dissertations

Food Science

Doctoral Dissertations

2007

Articles 1 - 5 of 5

Full-Text Articles in Life Sciences

The Stability Of Carotenogenic Food Colorants And Strategies To Prolong The Shelf Life In Process Cheese Spread, Ann Marie Doneski Craig Dec 2007

The Stability Of Carotenogenic Food Colorants And Strategies To Prolong The Shelf Life In Process Cheese Spread, Ann Marie Doneski Craig

Doctoral Dissertations

Food quality is often times measured by the way one perceives the food, particularly with respect to color and texture. Color quality often pre-determines expectation making it an important parameter to understand. The same can be said for textural properties of food. Maintaining color and texture are just two ways in which the shelf-life of food can be measured. One particular product that has experienced problems in this area is processed cheese spread in which the US military uses as part of the Meal, Ready-to-Eat (MRE) rations. The cheese spread is one of the most highly accepted products in the ...


Gene Expression Analysis Of Escherichia Coli O157:H7 At 10 And 37°C And Under Acidic Conditions Using High Density Oligonucleotide Microarrays, Kristina K. Carter Dec 2007

Gene Expression Analysis Of Escherichia Coli O157:H7 At 10 And 37°C And Under Acidic Conditions Using High Density Oligonucleotide Microarrays, Kristina K. Carter

Doctoral Dissertations

This purpose of this investigation was to use DNA microarrays to study gene expression in E. coli O157:H7 under refrigerated and acidic conditions. Total RNA from E. coli O157:H7 grown to 7 log CFU/mL under control (37 °C, pH 7), refrigerated (10°C, pH 7), acid adapted (37°C, pH 5.5, then lowered to 3.5), and acid shocked (37°C, pH 7, then lowered to 3.5) conditions was extracted using an optimized Qiagen RNeasy procedure. Total RNA was converted to cDNA, labeled, and hybridized onto an Affymetrix GeneChip® E. coli Genome 2.0 Array ...


Sonochemical And Hydrophobic Modification Of Chitin And Chitosan, Tao Wu Dec 2007

Sonochemical And Hydrophobic Modification Of Chitin And Chitosan, Tao Wu

Doctoral Dissertations

Chitin and chitosan are linear polysaccharides consisting of acetyl-glucosamine and glucosamine units with many potential applications in biomedicine, agriculture and food industry. In the food industry, chitosan can be used as antimicrobial agent and to create active packaging to improve food quality and extend shelf life. Commercial applications of chitosan are closely associated with its functional properties and biological activity, which are primarily governed by two structural properties: degree of acetylation (DA) and molecular weight (MW). The overall goal of this study was to develop methods for tailored modification of DA, MW and functionalities of chitosan.

The research was conducted ...


Application Of Solid Phase Micro-Extraction With Gas Chromatography-Mass Spectrometry For The Determination Of Geosmin And 2-Methylisoborneol In Processed Navy Beans (Phaseolus Vulgaris), Coesha Ancoinette Fairley Aug 2007

Application Of Solid Phase Micro-Extraction With Gas Chromatography-Mass Spectrometry For The Determination Of Geosmin And 2-Methylisoborneol In Processed Navy Beans (Phaseolus Vulgaris), Coesha Ancoinette Fairley

Doctoral Dissertations

This investigation was conducted to address the issue of musty bean products. The main objectives of this study were to: (1) identify the source of the musty, off-flavor in processed navy beans, (2) determine and identify the associated off-flavor compounds (geosmin and 2-methylisoborneol (2-MIB)), and (3) isolate the microorganism(s) responsible for production of the compounds.

To confirm the presence of geosmin and 2-MIB in processed baked beans, an automated gas chromatographic method employing solid phase micro-extraction (SPME-GC-MS) was used. Artificially musty baked beans, known musty beans, and raw ingredients (bacon, navy beans, and water) were analyzed. Results of this ...


Development Of A Real-Time Pcr Assay For Detection And Quantification Of Escherichia Coli O157:H7 In Apple Juice, Nancy Rabalais Detrana May 2007

Development Of A Real-Time Pcr Assay For Detection And Quantification Of Escherichia Coli O157:H7 In Apple Juice, Nancy Rabalais Detrana

Doctoral Dissertations

Apple cider/juice contaminated with Escherichia coli O157:H7 has been implicated in several foodborne illness outbreaks, but due to the presence of reaction inhibitors, detection by polymerase chain reaction (PCR) is often difficult. The studies presented in this dissertation were conducted to evaluate techniques to improve detection of E. coli O157:H7 in apple juice using a fluorogenic probe-based real-time PCR assay without prior enrichment. Two commercial DNA extraction and purification procedures, GenEluteTM Bacterial Genomic DNA Kit and PrepMan® Ultra Sample Preparation Reagent, were combined with two real-time PCR chemistries, SYBR® Green I dye and TaqMan® probes for potential ...