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Full-Text Articles in Life Sciences

Genomic Characterization Of Dairy Associated Leuconostoc Species And Diversity Of Leuconostocs In Undefined Mixed Mesophilic Starter Cultures, Cyril A. Frantzen, Witold Kot, Thomas B. Pedersen, Ylva M. Ardö, Jeff R. Broadbent, Horst Neve, Lars H. Hansen, Fabio Dal Bello, Hilde M. Østlie, Hans P. Kleppen, Finn K. Vogensen, Helge Holo Feb 2017

Genomic Characterization Of Dairy Associated Leuconostoc Species And Diversity Of Leuconostocs In Undefined Mixed Mesophilic Starter Cultures, Cyril A. Frantzen, Witold Kot, Thomas B. Pedersen, Ylva M. Ardö, Jeff R. Broadbent, Horst Neve, Lars H. Hansen, Fabio Dal Bello, Hilde M. Østlie, Hans P. Kleppen, Finn K. Vogensen, Helge Holo

Nutrition, Dietetics, and Food Sciences Faculty Publications

Undefined mesophilic mixed (DL-type) starter cultures are composed of predominantly Lactococcus lactis subspecies and 1–10% Leuconostoc spp. The composition of the Leuconostoc population in the starter culture ultimately affects the characteristics and the quality of the final product. The scientific basis for the taxonomy of dairy relevant leuconostocs can be traced back 50 years, and no documentation on the genomic diversity of leuconostocs in starter cultures exists. We present data on the Leuconostoc population in five DL-type starter cultures commonly used by the dairy industry. The analyses were performed using traditional cultivation methods, and further augmented by next-generation DNA ...


Development Of A Rapid Detection And Quantification Method For Yeasts And Molds In Dairy Products, Brandon Nguyen Jan 2017

Development Of A Rapid Detection And Quantification Method For Yeasts And Molds In Dairy Products, Brandon Nguyen

Dissertations, Theses, & Student Research in Food Science and Technology

A rapid quantitative PCR (qPCR) method was developed for the detection and quantification of fungi that are potentially present in dairy commodities. Genes of interest that were considered and used in the method development were the following: 18S rRNA, actin, beta-tubulin, and elongation factor 1-alpha. The following organisms were screened in this method development: Galactomyces candidus, Debaryomyces hansenii, Yarrowia lipolytica, Penicillium roqueforti, Penicillium verrocosum and Cladosporium cladosporioides. The developed method has a standard curve based on the organism, Galactomyces candidus, and the primers based on the elongation factor 1-alpha gene. Using this yeast and primers, this method can detect fungal ...


Development And Implementation Of Methods To Study Crystallization In Cheese, Gil Fils Tansman Jan 2017

Development And Implementation Of Methods To Study Crystallization In Cheese, Gil Fils Tansman

Graduate College Dissertations and Theses

Dissolved compounds and ions, including mineral elements and products of microbial metabolism, are present in many cheeses in relatively high concentrations. These dissolved substances may precipitate from the aqueous phase of cheese to form sparingly soluble crystals that can impart a crunchy, gritty, or sandy texture on the cheese. In the present work, optical and diffractometric methods were optimized for use with cheese samples to identify crystal phases in several cheese varieties. These techniques, which included powder X-ray diffractometry (PXRD), single crystal X-ray diffractometry (SCXRD), and petrographic microscopy (PM) have traditionally been used on geological specimens that are quite different ...