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Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

Analyzing Cellular Internalization Of Nanoparticles And Bacteria By Multi-Spectral Imaging Flow Cytometry, Yashdeep Phanse, Amanda E. Ramer-Tait, Sherree L. Friend, Brenda Carrillo-Conde, Paul Lueth, Carrie J. Oster, Gregory J. Phillips, Balaji Narasimhan, Michael J. Wannemuehler, Bryan H. Bellaire Jun 2012

Analyzing Cellular Internalization Of Nanoparticles And Bacteria By Multi-Spectral Imaging Flow Cytometry, Yashdeep Phanse, Amanda E. Ramer-Tait, Sherree L. Friend, Brenda Carrillo-Conde, Paul Lueth, Carrie J. Oster, Gregory J. Phillips, Balaji Narasimhan, Michael J. Wannemuehler, Bryan H. Bellaire

Faculty Publications in Food Science and Technology

Nanoparticulate systems have emerged as valuable tools in vaccine delivery through their ability to efficiently deliver cargo, including proteins, to antigen presenting cells1-5. Internalization of nanoparticles (NP) by antigen presenting cells is a critical step in generating an effective immune response to the encapsulated antigen. To determine how changes in nanoparticle formulation impact function, we sought to develop a high throughput, quantitative experimental protocol that was compatible with detecting internalized nanoparticles as well as bacteria. To date, two independent techniques, microscopy and flow cytometry, have been the methods used to study the phagocytosis of nanoparticles. The high throughput nature ...


Harvesting Murine Alveolar Macrophages And Evaluating Cellular Activation Induced By Polyanhydride Nanoparticles, Ana V. Chavez-Santoscoy, Lucas M. Huntimer, Amanda Ramer-Tait, Michael Wannemueler, Balaji Narasimhan Jun 2012

Harvesting Murine Alveolar Macrophages And Evaluating Cellular Activation Induced By Polyanhydride Nanoparticles, Ana V. Chavez-Santoscoy, Lucas M. Huntimer, Amanda Ramer-Tait, Michael Wannemueler, Balaji Narasimhan

Faculty Publications in Food Science and Technology

Biodegradable nanoparticles have emerged as a versatile platform for the design and implementation of new intranasal vaccines against respiratory infectious diseases. Specifically, polyanhydride nanoparticles composed of the aliphatic sebacic acid (SA), the aromatic 1,6-bis(p -carboxyphenoxy)hexane (CPH), or the amphiphilic 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) display unique bulk and surface erosion kinetics1,2 and can be exploited to slowly release functional biomolecules (e.g., protein antigens, immunoglobulins, etc.) in vivo3,4,5. These nanoparticles also possess intrinsic adjuvant activity, making them an excellent choice for a vaccine delivery platform6,7,8.

In order ...


A Longitudinal Study Of The Establishment And Proliferation Of Enterococcus On A Dairy Farm, Ginger M. Shipp, James S. Dickson May 2012

A Longitudinal Study Of The Establishment And Proliferation Of Enterococcus On A Dairy Farm, Ginger M. Shipp, James S. Dickson

Animal Science Publications

Enterococci are Gram-positive, facultative anaerobic cocci. They are found in many environments (including milk and dairy products, vegetables, plants, cereals, and meats). Enterococci are considered commensal organisms, but can also be opportunistic pathogens associated with morbidity and mortality of humans and animals. A longitudinal study of antibiotic resistance of Enterococcus to ampicillin, erythromycin, and tetracycline was conducted on an academic teaching farm. Environmental samples were collected by drag swabs at select locations prior to and after the introduction of livestock. All samples were initially processed and screened with specialized media, and then replica plated on tryptic soy agar containing a ...