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Full-Text Articles in Life Sciences

Development Of An Electrowetting Valve In Capillary-Driven Microfluidic Biosensor For Nucleic Acid Detection, Fei He, Tiphaine Mérian, Julie M. Goddard, Sam R. Nugen May 2012

Development Of An Electrowetting Valve In Capillary-Driven Microfluidic Biosensor For Nucleic Acid Detection, Fei He, Tiphaine Mérian, Julie M. Goddard, Sam R. Nugen

UMass Center for Clinical and Translational Science Research Retreat

This article presents the development of microfluidic valves to be used in capillary flow microfluidic device as a platform for nucleic acid detection. The valve used the principle of electrowetting and was able to be actuated at low voltage. The valve consisted of two silver electrodes which were encountered in series within a microfluidic channel. The second electrode was modified with a hydrophobic monolayer resulting in a cessation of capillary flow. A potential of 4V resulted in a 70° reduction in water contact angle within ten seconds which allowed capillary flow to continue. The final device represented a microfluidic valve ...


Development Of Lateral Flow Fluorescence Assay For The Detection Of Trypanosoma, Yuhong Wang, Sam Nugen May 2012

Development Of Lateral Flow Fluorescence Assay For The Detection Of Trypanosoma, Yuhong Wang, Sam Nugen

UMass Center for Clinical and Translational Science Research Retreat

Trypanosoma such as Trypanosoma brucei and Trypanosoma cruzi, the causative agents for African sleeping sickness and Chagas disease, respectively, have important influence on human health. The methods such as microscopic examination, immunological methods, and molecular methods are used for the identification and detection of Trypanosoma, but none of these methods are ideal to mass screening of samples such as onset of outbreak, epidemiological surveys, and blood unit screening. Therefore, there is a need for an assay which can rapidly, sensitively and specifically detect Trypanosoma.

In this study, Ru(bpy)32+-doped silica nanoparticles (RuSNP) were used to target nucleic ...


Development Of Markets For Local Food Crop To Enhance Incomes And Improve Food Security For Smallholder Farmers In East Africa, Betty Bugusu Apr 2012

Development Of Markets For Local Food Crop To Enhance Incomes And Improve Food Security For Smallholder Farmers In East Africa, Betty Bugusu

Changing World Conference

No abstract provided.


Analysis Of Zinc And Copper In Foods, Merlly Zavala Apr 2012

Analysis Of Zinc And Copper In Foods, Merlly Zavala

Undergraduate Research Conference

Deficiencies in vitamins and calcium have always been known and a remedy for it has always been around. The deficiency of copper (Cu) and zinc (Zn) are not very popular and some people do not understand how important the two metals are for one’s health. If one tends to encounter such deficiency the goal is to give foods that contain these metals in order to add to the diet. In this study, various foods were analyzed for Cu or Zn concentration levels using absorptions spectrophotometer Shimadzu AA-6800 flame. The samples were crushed, digested with nitric acid, and then analyzed ...